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71.
1. Germ-free and conventional Japanese quail reared on a diet in which the nitrogen was supplied solely in the form of free amino acids grew well, but growth was significantly better in both environments when some of the amino acids were replaced by an equivalent quantity of isolated soya protein. 2. Although the small intestine was shorter in the birds given the soya protein supplement, its weight per unit of length was greater; histological examination suggested that its circumference and wall thickness were both increased. 3. Results of a small experiment with conventional quail given the diets marked with 14C-polyethylene glycol indicated that the free amino acid diet passed through the gut more rapidly than the supplemented diet. 4. It was concluded that the growth-promoting effect of the isolated soya protein was unrelated to the activities of the gut microflora but might be due to changes in the digestive and absorptive capacities of the small intestine induced by the different physical nature of the supplemented diet. 相似文献
72.
Sequential study of lesion development in experimental haemophilus pleuropneumonia 总被引:18,自引:0,他引:18
Recently weaned pigs were infected aerogenically with Haemophilus (Actinobacillus) pleuropneumoniae, serotype 5. At three, six, 12, and 18 hours and one, two, four and seven days after exposure to haemophili a pair of animals were killed and necropsied. Pulmonary oedema with multifocal petechial haemorrhages and a diffuse neutrophilic bronchiolitis and alveolitis were observed at three and six hours after infection. Focal areas of coagulative necrosis developed in areas of intense suppuration at 12 and 18 hours after infection. At one and two days after infection, necrotic areas were surrounded by dense bands of degenerating leucocytes and contained unidentifiable round and elongated cells characteristic of this disease. In subacute lesions a thick layer of granulation tissue formed around the outer margin of developing abscesses. Most of the round and elongated cells in alveolar exudates could not be identified by enzyme histochemistry or electron microscopic examination. Neutrophils in various stages of degeneration and deterioration provided strong evidence that some of the cells were of this type. These findings suggest that neutrophils may play an early and significant role in development of lesions. 相似文献
73.
74.
Ovarian and gonadotropin responses to insulin and energy restriction were investigated in a 2 X 2 factorial experiment using 2-yr-old Brangus heifers. Thirty heifers were paired by weight and body condition, then assigned to treatment groups receiving 75 (LE) or 180% (HE) of NRC recommendations for dietary energy for maintenance. Diets were adjusted weekly to maintain daily .25 to .5 kg weight loss or 0 to .25 kg weight gain, respectively. On d 10 of the first estrous cycle subsequent to the initial 45 d of feeding, heifers within each dietary group were allocated to receive twice daily infusions of either 40 U insulin (I) or saline (C). Infusions began at 5 and 10 h postprandial and were given in six boluses, 20 min apart. Infusions continued daily until d 20 or estrus, whichever occurred first. On d 11, blood samples were collected at 15-min intervals for 12 h to determine luteinizing hormone (LH) and insulin concentrations. On d 16 to 20, twice daily im injections of 1 mg follicle stimulating hormone (FSH) were administered. Heifers were ovariectomized on d 11 after estrus. Number of corpora lutea (CL) in LE-I heifers was greater (P less than .05) in LE-C, HE-C or HE-I. Total CL weight (g) per heifer was greater (P less than .05) in HE-C and LE-I heifers than in LE-C. Individual CL wt was heavier in HE than in LE heifers (P less than .05).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
75.
RAP Harrison 《Reproduction in domestic animals》2003,38(2):102-110
Cyclic AMP is known to play a major role in intracellular signalling during mammalian sperm capacitation. However, despite much research, many of the molecular details of cyclic AMP's involvement remain obscure. In this review, I discuss the following aspects, presenting some original data as illustration where relevant. With respect to cyclic AMP synthesis, uncertainties exist as to the number of forms of adenylyl cyclase that are present in the spermatozoon, whether they are cytosolic or bound to subcellular structures, and which physiological effectors they respond to (e.g. bicarbonate, Ca2+, or receptor‐coupled G‐proteins). While net intracellular levels of cyclic AMP in spermatozoa depend upon the relative activities of adenylyl cyclase and phosphodiesterase, there are wide between‐sample variations within species, both in basal levels and in levels attained after activation of the cyclase (e.g. after sperm treatment with bicarbonate). Moreover, minor changes in bulk cyclic AMP levels can result in large changes in cyclic AMP‐dependent functions. Finally, while cyclic AMP levels respond very rapidly to sperm treatment by effectors such as bicarbonate and Ca2+ (key components of capacitating media), there are big discrepancies between the rates of functional response. For example, enhancement of motility and collapse of phospholipid asymmetry take place within a few minutes, whereas more than 1 h of exposure to capacitating conditions is needed for cyclic AMP‐dependent protein tyrosine phosphorylation to become detectable or for the sperm population to attain a capacitated state. 相似文献
76.
L. J. S. Harrison J. A. Hammond M. M. H. Sewell 《Tropical animal health and production》1996,28(1):23-39
Summary The research work relating to helminths, which has been conducted within the Helminthology Section of the CTVM, often in collaboration with colleagues from the tropics is reviewed and placed into a historical perspective. The research has, in the main, concentrated on the trematodesFasciola hepatica andFasciola gigantica and the cestodesTaenia saginata andTaenia solium, but work on other parasites including gastro-intestinal nematodes is also considered. All of these parasites are of obvious veterinary/economic importance particularly in the tropics and subtropics. While the zoonotic importance ofT. saginata andT. solium has been recognised for many years, it is only more recently that the zoonotic impact ofFasciola spp. has been generally acknowledged.
Estudios Sobre Enfermedades Causadas Por Helmintos En El CTVM
Resumen El artículo revisa y sitúa en una perspectiva histórica la investigación sobre helmintos realizada en la sección de helmintología del CTVM, a menudo en colaboración con otros investigadores de países tropicales. La investigación se ha centrado principalmente en los trematodosFasciola hepatica y Fasciola gigantica y en los cestodosTaenia saginata y Taenia solium, aunque en el artículo se incluye también el trabajo realizado con nematodos gastrointestinales y otros parásitos. Todos los parásitos mencionados tienen una importancia obvia desde el punto de vista veterinario y económico, particularmente en los trópicos y subtrópicos. Mientras que la importancia deT. saginata yT. solium como zoonosis ha sido siempre reconocida, el posible carácter zoonótico deFasciola spp. ha sido descubierto muy recientemente.
Etudes Sur Les Helminthiases A CTVM
Résumé Le travail de recherche portant sur les helminthes, qui a été mené dans le groupe Helminthologie à CTVM, souvent en collaboration avec des collègues des pays tropicaux, est passé en revue et mis dans une perspective historique. La recherche a principalement porté sur les trematodesFasciola hepatica etFasciola gigantica et sur les cestodesTaenia saginata etTaenia solium, cependant d'autres travaux ont également porté sur d'autres parasites et sur des nématodes gastro-intestinaux.Tous ces parasites sont d'une importance économique/vétérinaire évidente et ceci particulièrement dans les pays tropicaux et sub-tropicaux. Tandis que l'importance deT. saginata etT. solium, sur la zoonose, a toujours été reconnue, ce n'est que récemment que l'impact deFasciola spp sur la zoonose a été repertorié.相似文献
77.
Absorption of cysticerci in cattle after treatment of taenia saginata cysticercosis with praziquantel 总被引:1,自引:0,他引:1
Two groups of calves aged 10 to 15 weeks were orally infected with either 6000 or 30,000 Taenia saginata eggs. Three to four months later they were treated with praziquantel, three doses of 50 mg kg-1 on three consecutive days. All the cysticerci were killed by the drug and most were absorbed by between six and nine months after treatment. From then on there was little further absorption and residual calcified cysts were still found in the carcases up to 24 months after drug treatment. These residual calcified cysts were particularly common in the heart muscles and would probably have been detected at meat inspection. 相似文献
78.
79.
The serum antibody levels to Taenia saginata of three groups of cattle were assessed by an enzyme-linked immunosorbent assay (ELISA). The first group of cattle were from four farms which had a confirmed T saginata cysticercosis outbreak, all of which had cattle classed as infected by ELISA. The second group were from four farms where sewage sludge had been applied to pasture subsequently grazed by the cattle. One of these farms had cattle classed as infected by ELISA. The control cattle, which were all classed as uninfected by ELISA, came from five farms whose pasture had not been treated with sewage sludge. In a wider survey, involving sera from 47 additional farms, the majority could not be distinguished from the control farms in the earlier survey. However, samples from three of the farms had a similar number of positives to two of the known infected farms in the initial survey. Since the ELISA assay may indicate infected herds, farms such as these warrant further investigation. 相似文献
80.