Defining the typical aroma of sherry vinegar: sensory and chemical approach     

Callejón RM  Morales ML  Ferreira AC  Troncoso AM 《Journal of agricultural and food chemistry》2008,56(17):8086-8095

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Systematic exploration of the equine tarsus by ultrasonography     

Vilar JM  Rivero MA  Arencibia A  Morales I  Pinedo M 《Anatomia, histologia, embryologia》2008,37(5):338-343

The authors propose a standard protocol of ultrasonographic exploration of the tarsal region in transversal and longitudinal planes, with the aim to spread with an accurate location of the anatomical structures. For this study, 10 adult horses of different races and ages (ranged 5-10 years) were used, with the characteristic of not having suffered any kind of tarsic pathology. The equipment was a real time ultrasound machine with a 7.5 mHz lineal transducer. Some different anatomical structures in this region of the hind-limb such as the tendons, ligaments, muscles and articular components were identified and labelled as regards their echoic characteristics.  相似文献   

Molecular identification of Echinococcus granulosus genotypes (G1 and G7) isolated from pigs in Mexico     

Villalobos N  González LM  Morales J  de Aluja AS  Jiménez MI  Blanco MA  Harrison LJ  Parkhouse RM  Gárate T 《Veterinary parasitology》2007,147(1-2):185-189

With the aim of genotyping Echinococcus granulosus cysts found in Mexican livestock, we collected hydatid cysts from the livers and lungs of pigs in slaughterhouses in the state of Morelos, Central Region of Mexico. DNA was extracted from the parasites and examined by polymerase chain reaction (PCR) of rDNA internal transcribed spacer 1 (ITS1-PCR), Eg9-PCR, Eg16-PCR, and PCR-restriction fragment length polymorphism (PCR-RFLP). In addition, fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (CO1) and NADH dehydrogenase 1 (ND1) were sequenced. Two different genotypes of E. granulosus were unequivocally identified, the common sheep genotype, G1, and the common pig genotype, G7. The G1 genotype of E. granulosus has not been previously demonstrated in Mexico. Because of its recognized infectivity in humans, G1 genotype is a direct threat to human health and its presence in Mexico is consequently of immediate public health importance and epidemiological relevance.  相似文献   

Organic matrix composition and ultrastructure of eggshell: a comparative study.   总被引：4，自引：0，他引：4  

M Panheleux  M Bain  M S Fernandez  I Morales  J Gautron  J L Arias  S E Solomon  M Hincke  Y Nys 《British poultry science》1999,40(2):240-252

1. The avian eggshell is a biomineralised composite ceramic consisting of calcium carbonate embedded in an organic matrix. Matrix components are supposed to be involved in the control of mineralisation, crystallographic texture and biomechanical properties of eggshell. 2. The structure and eggshell matrix composition of various domesticated bird species were compared to gain insight into the universality of the eggshell mineralisation process. 3. The SDS-PAGE profiles of soluble eggshell matrix were specific within groups of birds (a: laying hen, breeder hen, quail, pheasant and possibly turkey; b: guinea fowl; c: duck and goose) but some of the protein bands were common to all groups. 4. Analogies between species were confirmed by Western blotting using hen protein antibodies. Ovocleidin-17 (OC-17) and ovalbumin were revealed in all species (except quail for OC-17). Lysozyme was present only in hen eggshell. Another egg white protein: ovotransferrin showed a positive signal in hens, turkey and quail. Osteopontin was observed in laying and breeder hens and quail. 5. Different proteoglycans were localised to discrete regions within the eggshell. Dermatan sulphate was observed within the matrix of the calcified shell of all species except quail which contained chondroitin-6-sulfate. Keratan sulphate was observed in mammillary bodies of breeder and laying hen, quail, pheasant and turkey while chondroitin sulphate was also present in guinea fowl and duck. 6. The general structural organisation of the different avian eggshells was similar but specific differences were observed in the ultrastructure of the mammillary layer. Species of the same taxonomic family could be grouped according to their structural analogies: breeder hen, turkey and pheasant resembled that of the domestic fowl. Guinea fowl was unique. Goose and duck were quite similar with large and confluent mammillary bodies. 7. Some matrix components are therefore common to eggshells of various species but more information is needed to relate differences in matrix composition between taxonomic groups with differences in ultrastructure.  相似文献   

Levels of circulating plasma immune complexes after thiacetarsamide treatment in dogs naturally infected with Dirofilaria immitis.     

J M Molina  A Ruiz  O Ferrer  M C Mu?oz  J Orós  M Morales 《The Veterinary record》1999,144(18):506-507

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First identification and characterization of Tenacibaculum dicentrarchi isolated from Chilean red conger eel (Genypterus chilensis,Guichenot 1848)          下载免费PDF全文

R Irgang  R González‐Luna  J Gutiérrez  M Poblete‐Morales  V Rojas  D Tapia‐Cammas  R Avendaño‐Herrera 《Journal of fish diseases》2017,40(12):1915-1920

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Significant improvement of shrimp growth performance by growth hormone‐releasing peptide‐6 immersion treatments          下载免费PDF全文

Amílcar Arenal  Juana María Lugo  Reynold Morales  Leonardo Martín  Ramón Franco Rodríguez  Jannel Acosta  Antonio Morales  Jorge Duconge  Mario Pablo Estrada 《Aquaculture Research》2017,48(9):4632-4645

Growth hormone‐releasing peptide‐6 (GHRP‐6) is one of the earliest developed synthetic peptidyl growth hormone secretagogue receptor agonists. These compounds mimic the effect of the endogenous ligand ghrelin. In vertebrates, ghrelin is a potent circulating orexigenic hormone with functional roles in controlling food intake, energy expenditure, adiposity, growth hormone secretion and immunity. Ghrelin has been studied mainly in vertebrates; thus, little is known about its role in invertebrates, including crustaceans. We first evaluated the effect of GHRP‐6 injection over feed intake in shrimp and its effects on shrimp growth when the peptide was administrated by successive immersion baths. GHRP‐6 increased feed intake, body weight and size, the number of rostral spines and gill branches, protein concentration and haemocyte number in treated shrimps. We also evaluated the peptide uptake and clearance in a pharmacokinetics, using [H³]GHRP‐6 administered to postlarvae. Given a limited exposure and efficient clearance of the peptide‐associated radioactivity from larvae, our findings suggested that GHRP‐6‐treated Litopenaeus vannamei can be consumed safely by humans after aquaculture applications. These results propose that GHRP‐6 could be an additional tool to study growth physiology in crustaceans and also a promising candidate for development into a new biotechnology product for improving shrimp growth and quality.  相似文献   

Evidence for the facultative intracellular behaviour of the fish pathogen Vibrio ordalii     

Ruben Avendao‐Herrera  Eloisa Arias‐Muoz  Vernica Rojas  Alicia E. Toranzo  Matías Poblete‐Morales  Claudio Crdova  Rute Irgang 《Journal of fish diseases》2019,42(10):1447-1455

Vibrio ordalii is an extracellular, Gram‐negative bacterium that produces vibriosis in salmonids. While pathogenesis is not fully understood, this bacterium has numerous likely genes for adhesion, colonization, invasion factors and, as recently suggested, intracellular behaviour. Therefore, this study aimed to clarify possible intracellular behaviour for V. ordalii Vo‐LM‐18 and ATCC 33509^T in the fish‐cell lines SHK‐1 and CHSE‐214. Confocal microscopy revealed Vo‐LM‐18 and ATCC 33509^T inside cytoplasm in both fish‐cell lines at 4 hr post‐inoculation (hpi). At 8 and 16 hpi, the proportion of fish cells invaded by both strains increased. Moreover, intracellular V. ordalii were observed after 8 hpi inside mouse embryonic fibroblasts (MEF), demonstrating that entry was not due to a cellular phagocytosis process. Flow cytometry confirmed immunocytochemistry results, with both V. ordalii evidencing statistically significant differences in the number of infected cells between 8 and 16 hpi. Interestingly, V. ordalii infection did not significantly damage fish cells, as determined by LDH liberation. Viable counts at 8 hpi detected, on average for both lines, 176 ± 47 CFU/ml of culturable intracellular Vo‐LM‐18 and ATCC 33509^T cells. These in vitro findings support the facultative intracellular behaviour of V. ordalii and may be of importance for understanding pathogenicity and survival in aquatic environments.  相似文献   

Efficacy of thiamethoxam and fipronil,applied alone and in combination,to control Limonius californicus and Hypnoidus bicolor (Coleoptera: Elateridae)          下载免费PDF全文

Anuar Morales‐Rodriguez  Kevin W Wanner 《Pest management science》2015,71(4):584-591

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Naegleria fowleri-associated encephalitis in a cow from Costa Rica     

Morales JA  Chaves AJ  Visvesvara GS  Dubey JP 《Veterinary parasitology》2006,139(1-3):221-223

Species of Naegleria, Acanthamoeba, and Balamuthia are soil amoebae that can cause encephalitis in animals and humans. Of these, Naegleria fowleri is the cause of often fatal primary meningoencephalitis in humans. N. fowleri-associated encephalitis was diagnosed in a cow that was suspected to have rabies. Only formalin-fixed brain was available for diagnosis. There was severe meningoencephalitis involving all parts of the brain and numerous amoebic trophozoites were present in lesions. The amoebae reacted with N. fowleri-specific polyclonal antibodies in an indirect immunofluorescent antibody test. This is the first report of amoebic encephalitis in any host from Costa Rica.  相似文献