Latrodectism in New Caledonia: First Report of Presumed Redback Spider (Latrodectus hasselti) Envenomation     

Claude Maillaud  Hervé Jourdan  Ken Winkel  Gaëlle Arnaud  Patrick Lafforgue  Francis Durand 《The Forestry Chronicle》2009,20(4):339-343

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Use of an acoustic telemetry array for fine scale fish behaviour assessment of captive Paiche,Arapaima gigas,breeders          下载免费PDF全文

Jesús Núñez‐Rodríguez  Antonia Vela Díaz  Roger Bazan‐Albitez  Carmela Rebaza Alfaro  Daniel Koua  Lucie Núñez  Baptiste Testi  Jean‐François Renno  Fabrice Duponchelle  Hervé Pella 《Aquaculture Research》2018,49(6):2296-2304

As Arapaima gigas is one of the most valuable species for the growing production of Amazonian aquaculture, knowledge of its reproductive behaviour and its application to increase reproduction success in captivity is of great importance as no hormonal spawning induction technique exists for this species. An acoustic positioning system (LOTEK Inc.) was used to observe the interactions of adult fish to better understand the formation of mating pairs. Fish were placed in a 4,500 m² aquaculture pond over a 6‐month period in the IIAP field station of Pucallpa, Perú. This paper describes the methodological protocols used to set up and test the hydrophone array and presents the methodology used for the analysis of the huge amount of collected data. This methodology is illustrated by the analysis of a 6‐day period for a mating pair that showed a spawning event. The results indicated that male and female occupied mostly one preferential area in one pond edge where the nesting area is located. Different activity patterns were observed during the spawning event, with male and female being closer during the spawning day. The results also showed that male travelled less distance than female during the studied period. Finally these results demonstrated the suitability of such equipment to monitor fish interactions at fine spatial (sub meter) and temporal (5 s) scales in confined environments like aquaculture ponds.  相似文献   

Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells     

Meret Elisabeth Ricklin Gutzwiller  Hervé Rapha?l Moulin  Andreas Zurbriggen  Petra Roosje  Artur Summerfield 《Veterinary research》2010,41(4)

Dendritic cells (DC) represent a heterogeneous cell family of major importance for innate immune responses against pathogens and antigen presentation during infection, cancer, allergy and autoimmunity. The aim of the present study was to characterize canine DC generated in vitro with respect to their phenotype, responsiveness to toll-like receptor (TLR) ligands and T-cell stimulatory capacity. DC were derived from monocytes (MoDC) and from bone marrow hematopoietic cells cultured with either Flt3-ligand (FL-BMDC) or with GM-CSF (GM-BMDC). All three methods generated cells with typical DC morphology that expressed CD1c, CD11c and CD14, similar to macrophages. However, CD40 was only found on DC, CD206 on MΦ and BMDC, but not on monocytes and MoDC. CD1c was not found on monocytes but on all in vitro differentiated cells. FL-BMDC and GM-BMDC were partially positive for CD4 and CD8. CD45RA was expressed on a subset of FL-BMDC but not on MoDC and GM-BMDC. MoDC and FL-DC responded well to TLR ligands including poly-IC (TLR2), Pam3Cys (TLR3), LPS (TLR4) and imiquimod (TLR7) by up-regulating MHC II and CD86. The generated DC and MΦ showed a stimulatory capacity for lymphocytes, which increased upon maturation with LPS. Taken together, our results are the basis for further characterization of canine DC subsets with respect to their role in inflammation and immune responses.  相似文献   

Breeding potential of Belgian landraces of leek ( Allium ampeloprasum L. var. porrum)     

Hervé De Clercq  Joost Baert  Erik Van Bockstaele 《Euphytica》1999,106(2):101-109

Western Europe is the world's greatest leek producing and consuming region. Belgium is situated in the centre of Western Europe and can be considered as a genepool of landraces of leek. Although the commercial varieties nowadays take over the successes of Belgian landraces, this paper deals with the breeding potential of the Belgian landraces. Typing the Belgian landraces was done in an observation trial together with 50 cultivars. Clustering analysis revealed that 4 of the Belgian landraces were autumn types, 2 landraces were of the early winter type and another 12 landraces were ranged with 6 commercial varieties to form the most winterhardy group of leek. For the important characteristics leaf colour and senescence resistance, the Belgian landraces were better than most of the commercial varieties. Although, for economically more important characteristics shaft length and some disease resistance they scored low. Finally, in this trial, the Belgian landraces were high yielding, especially in the late season. But also in the early season (harvest in September and November) some Belgian landraces were in the top 10 for yield. At harvest in February 9 of the 10 best yielding winterleeks were Belgian landraces. This proves that the Belgian landraces were well adapted to the soil and climate of this region. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

The use of continuous light to suppress pre‐harvest sexual maturation in sea‐reared Atlantic salmon (Salmo salar L.) can be reduced to a 4‐month window     

Eric Leclercq  Hervé Migaud  John F Taylor  Dougie Hunter 《Aquaculture Research》2010,41(10):e709-e714

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Effects of river infrastructures on the floodplain sedimentary environment in the Rhône River     

Vauclin  Sophia  Mourier  Brice  Tena  Alvaro  Piégay  Hervé  Winiarski  Thierry 《Journal of Soils and Sediments》2020,20(6):2697-2708

Journal of Soils and Sediments - River infrastructures such as dikes, groynes, and dams are ubiquitous on most large rivers, and although their consequences on the riverbed morphology have often...  相似文献   

Administration of Duddingtonia flagrans chlamydospores to goats to control gastro-intestinal nematodes: dose trials     

Paraud C  Hoste H  Lefrileux Y  Pommaret A  Paolini V  Pors I  Chartier C 《Veterinary research》2005,36(2):157-166

The ability of the nematophagous fungus Duddingtonia flagrans to reduce the number of infective nematode larvae in coproculture was investigated in goats using different doses of chlamydospores (0, 1.25 x 10(5), 2.5 x 10(5), 5 x 10(5) chlamydospores/kg BW/day) given by oral administration or by voluntary consumption in feed during natural or experimental infections with nematodes. The kinetics of excretion of D. flagrans chlamydospores in the faeces was also determined using a dose of 5 x 10(5) chlamydospores/kg BW/day for five days. For all the trials, the faecal nematode egg outputs were determined by a modified McMaster method and standard coprocultures were set up (14 days, 25 degrees C) to determine the number of larvae emerging from culture in fungus treated and control faeces. When chlamydospores were orally administered, the number of larvae were reduced by 50 to 97% when compared to control cultures. No difference in the level of larval emergence from the culture was seen for experimental or natural infections at the different chlamydospore dose rates. In contrast, when chlamydospores were distributed in the feed, a dose-dependent relationship was observed 10 days after the start of administration, the larval development being 2.0%, 14.0% and 86.9% for 5 x 10(5), 2.5 x 10(5) and 0 spores/kg BW/day, respectively. In addition, the kinetic study showed that the larval emergence from coproculture in the fungus group was statistically lower than in the control group from the second day of administration of the chlamydospores and remained lower until the second day after the last administration (p < 0.05). The results indicate that, for goats in farm conditions, a minimum daily dose of 5 x 10(5) chlamydospores/kg BW must be used to ensure a high treatment efficacy and that daily administration is preferable for maintenance of efficacy over time.  相似文献   

Quantification, repeatability, and reproducibility of feline radial and longitudinal left ventricular velocities by tissue Doppler imaging     

Chetboul V  Athanassiadis N  Carlos C  Nicolle AP  Tissier R  Pouchelon JL  Concordet D  Lefebvre HP 《American journal of veterinary research》2004,65(5):566-572

OBJECTIVE: To measure the radial and longitudinal velocities of several myocardial segments of the left ventricular wall by use of tissue Doppler imaging (TDI) in healthy cats and determine the repeatability and reproducibility of the technique. ANIMALS: 6 healthy cats. PROCEDURE: 72 TDI examinations were performed on 4 days by the same trained observer. Radial parameters included left endocardial and epicardial myocardial velocities. Longitudinal parameters included left basal, middle, and apical myocardial velocities. RESULTS: All velocity profiles had 1 positive systolic wave (S) and 2 negative diastolic waves (E and A). Myocardial velocities were higher in the endocardial than epicardial segments during the entire cardiac cycle (systolic wave S, 4.4 +/- 0.82 and 1.9 +/- 0.55; diastolic wave E, 9.7 +/- 1.70 and 2.2 +/- 0.74; and diastolic wave A, 5.1 +/- 1.56 and 1.4 +/- 0.76, respectively). Velocities were also higher in the basal than in the apical segments (systolic wave S, 4.7 +/- 0.76 and 0.2 +/- 0.11; diastolic wave E, 9.7 +/- 1.36 and 0.5 +/- 0.17; and diastolic wave A, 3.7 +/- 1.51 and 0.2 +/- 0.13, respectively). The lowest within-day and between-day coefficients of variation were observed in endocardial segments (8.2% and 6.5% for systolic wave S and diastolic wave E, respectively) and in the basal segment in protodiastole (5.5%). CONCLUSIONS AND CLINICAL RELEVANCE: Repeatability and reproducibility of TDI were adequate for measurement of longitudinal and radial left ventricular motion in healthy awake cats. Validation of TDI is a prerequisite before this new technique can be recommended for clinical use.  相似文献   

Plant species response to urbanization: comparison of isolated woodland patches in two cities of North-Western France   总被引：1，自引：0，他引：1  

Jeanne Vallet  Hervé Daniel  Véronique Beaujouan  Françoise Rozé 《Landscape Ecology》2008,23(10):1205-1217

The effect of urbanization on species distribution has been extensively documented, but a main challenge in urban ecology is to better understand the factors causing different distributions among species in response to urbanization. Hence, this paper aims to compare the effects of urbanization on woodland plant assemblages in two cities and to describe species responses by using several indicators. The study was carried out in the cities of Angers and Rennes (North-Western France) where 11 isolated woodlands were surveyed along an urban–rural gradient in each city. Abundance data of spontaneous species were collected from 220 quadrats. The effect of land cover (within a 500 m buffer around each woodland) on species assemblages was investigated by Canonical Correspondence Analysis. Buildings and pavement areas were the most significant predictors of species composition, and the effect of location in Angers or Rennes appeared on the second axis. More than 60% of the most frequent plant species were indicator of urban or rural location and their preferences were similar in the two cities. These lists of urban and rural indicator species were compared with Ellenberg’s indicator values and two other indicators specific to forest environment. The species which grow preferentially in urban woodlands are species which are already known to be associated with recent forests rather than ancient forests; with hedgerows rather than woodlands. The opposite pattern was observed concerning rural species. Moreover, urban indicator species have higher optima for soil pH and soil nitrogen content than rural indicator species. Different characteristics and history of forest habitat—continuity of the forest land cover, linearity of the habitat, change in adjacent land cover and land use—could select the same species, and the responses of the latter might involve different preferences concerning soil alkalinity and nutrient status.  相似文献   

Fractional excretion tests: a critical review of methods and applications in domestic animals     

Lefebvre HP  Dossin O  Trumel C  Braun JP 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(1):4-20

The fractional excretion (FE) of a constituent by the kidney is the fraction of the amount filtered by the glomerulus, which is excreted into urine. It is mostly determined for electrolytes, and is expressed as the ratio of the clearance of a given electrolyte to creatinine clearance. The main physiologic factors affecting FE variation are species, age, and the alimentary supply of electrolytes. The value of FE tests in the diagnosis of kidney disease is limited, except in canine Fanconi's syndrome. FEs of many constituents often are increased in chronic kidney disease, but their diagnostic value is no greater than that of plasma creatinine concentration. FEs also are altered in nonrenal diseases such as diabetes mellitus and rhabdomyolysis, and during treatment with xylazine, rehydration fluids, and diuretics. FEs, especially of calcium, phosphates, and magnesium, are useful in clinical nutrition to assess mineral balance. FE is difficult to measure, so its use should be limited to nutritional investigations and nephrology research.  相似文献