Canopy transpiration in two Japanese cypress forests with contrasting structures     

Kenji Tsuruta  Hikaru Komatsu  Tomonori Kume  Yoshinori Shinohara  Kyoichi Otsuki 《Journal of Forest Research》2015,20(5):464-474

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Genetic Variation of Glutelin Acidic Subunit Polypeptides in Bangladesh Rice Genetic Resources     

Md. Sarwar?Jahan  Yuji?Uemura  Toshihiro?Kumamaru  Abdul?Hamid  Hikaru?SatohEmail author 《Genetic Resources and Crop Evolution》2005,52(8):977-987

Bangladesh rice genetic resources collected from six distinct regions of the country were examined to obtain the diversity in glutelin acidic subunit polypeptides. Seed glutelins from 576 Bangladesh rice cultivars representing seven ecotypes were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing (IEF) electrophoresis and two-dimensional electrophoresis (2-DE: SDS-PAGE/IEF) analyses. Glutelin acidic subunit was separated into four bands, α-1, α-2, α-3 and α-4, and the variation in each of bands was detected by SDS-PAGE analysis. A higher molecular size component of α-1(α-1′) was identified in the cultivars tested. In case of α-2, α-2H with high molecular mass, α-2L with low molecular mass and α-2H/L were detected. α-3 band variation showed α-3H, α-3L, and α-3H/L, while for α-4, α-4H, α-4L bands were identified. In IEF analysis, a total of 16 bands with independent pI ranging from pI 6.30 to 7.52 were identified for the glutelin acidic subunit among the cultivars. The maximum and minimum numbers of IEF bands found were 13 and 9, respectively. The α-2L less cultivars were also lacking in pI 6.80 polypeptide in IEF. Result of 2-DE showed that pI 6.80 polypeptide was the main component of α-2L band. Transplanted Aman ecotype was the most diverse with respect to glutelin variation. Geographical distribution of glutelin variation in the Transplanted Aman ecotype differed according to the regions. The above results indicate that Bangladesh rice cultivars possess great genetic diversity in glutelin acidic subunit polypeptides. This study of indigenous rice cultivars from Bangladesh provides useful information regarding their breeding potential.  相似文献   

Diversity of glutelin acidic subunit polypeptides in rice cultivars collected from Northern Vietnam     

Tran T. T. Hoai  Tran D. Suu  Hikaru Satoh  Toshihiro Kumamaru 《Plant Breeding》2014,133(3):341-347

The diversity of glutelin acidic polypeptides in rice cultivars collected from Northern Vietnam was characterized via sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS‐PAGE) and isoelectric focusing (IEF) electrophoresis. Glutelin acidic subunits were separated into four bands by molecular mass, as α‐1 (39 kDa), α‐2 (38 kDa), α‐3 (37.5 or 37 kDa) and α‐4 (34 or 33 kDa). One hundred and eighty‐five Vietnamese rice cultivars were divided into three types, based on differences in staining intensity and the molecular size of the α‐3 and α‐4 polypeptides derived from SDS‐PAGE analysis. Wide variation was also observed in the isoelectric point (pI) staining intensity, in addition to the absence/presence of pI bands detected via IEF analysis. A total of 16 pI bands, ranging from pI 6.30 to pI 7.52, were identified in the various local rice cultivars. The maximum and minimum of IEF bands detected were 14 and 10, respectively. The genetic variability index (H′) ranged from 0.280 to 0.820, which confirms that local rice cultivars from Northern Vietnam have diverse glutelin seed storage units.  相似文献   

Depletion of naturally recruited wild Japanese eels in Okayama,Japan, revealed by otolith stable isotope ratios and abundance indices     

Kenzo Kaifu  Kazuki Yokouchi  Tomihiko Higuchi  Hikaru Itakura 《Fisheries Science》2018,84(5):757-763

To investigate the population dynamics of naturally recruited wild Japanese eels, fisheries data of wild individuals in Okayama Prefecture were investigated as a case study. Wild and stocked eels were discriminated using a recently developed method based on otolith stable isotopes. Of the 161 eels captured in freshwater areas where eels had been stocked, 98.1% were discriminated as stocked. In contrast, 82.8% of 128 eels captured in coastal areas where eels are not stocked were discriminated as wild. There was a significant decrease in longline and set-net catch per unit effort between 2003 and 2016 in the coastal areas where most eels were discriminated as wild, indicating ongoing depletion of wild Japanese eels in these waters.  相似文献   

Development of acid soil conditioning agent from lignin by ozone treatment I   总被引：2，自引：2，他引：0  

Hikaru Aimi  Satoshi Ohmura  Tamao Kato  Tomoko Nakahara  Kazumasa Shimizu 《Journal of Wood Science》2008,54(3):214-219

A purified softwood kraft lignin was modified by ozone treatment and its activity as an acid soil conditioning agent, mainly focusing on elimination of aluminum toxicity, was assayed by planting experiments. The growth of radish root was examined in nutrient solution containing CaCl₂ and AlCl₃ at pH 4.8 with and without modified kraft lignins. The modified kraft lignins that absorbed 1.8 and 3.9 moles of ozone per C6-C3 unit (M _w 180) showed two effects: the elimination of aluminum toxicity and the acceleration of root growth. The effect on the elimination of aluminum toxicity was observed even with modified kraft lignin that absorbed 1.0 mole of ozone per C6-C3 unit. The high molecular weight part of the modified kraft lignin that absorbed 3.9 moles of ozone per C6-C3 unit also proved to be effective not only in elimination of aluminum toxicity but also in acceleration of root growth. The acceleration effect of ozone-treated lignins on root growth was also observed under the absence of aluminum in planting experiments. This report was presented in part at the 56th Annual Meeting of the Japan Wood Research Society, Akita, Japan, August 2006  相似文献   

Seasonal changes of nitrate concentrations in baseflow headwaters of coniferous forests in Japan: A significant indicator for N saturation     

Zhao Zhang  Takehiko Fukushima  Peijun Shi  Fulu Tao  Yuichi Onda  Takashi Gomi  Shigeru Mizugaki  Yuko Asano  Ken'ichirou Kosugi  Shinya Hiramatsu  Hikaru Kitahara  Koichiro Kuraji  Tomomi Terajima  Kazuo Matsushige 《CATENA》2008

Understanding the seasonal and regional patterns of nitrogen (N) components in the headwaters of forested watersheds is important for forests management. Here, we investigated the NO₃-N concentration and its seasonal variations in the baseflow headwaters in Japanese coniferous forests with different N saturation status in five regions (i.e., Nagano, Tokyo, Aichi, Kochi and Mie). We found that the ratios of NO₃-N to DON were significantly higher in the N-saturated regions including Nagano (14.1 ± 2.2) and Tokyo (16.5 ± 4.1), compared with the regions, which didn't experience N saturation including Aichi (1.4 ± 0.2), Kochi (5.1 ± 1.8), and Mie (2.2 ± 0.5). In comparison with the regions without experiencing N saturation, the relatively higher NO₃-N concentration, as well as its contrastive variation between growing season and dormant season, was also characteristic of the seasonal NO₃-N concentration in the N-saturated regions. Our findings have indicated that NO₃-N concentration, its seasonal variations, and the ratio of NO₃-N to DON, are the better and applicable indicators for evaluating N saturation status in Japanese coniferous forests.  相似文献   

Development of microsatellite markers in cultivated and wild species of sections <Emphasis Type="Italic">Cepa</Emphasis> and <Emphasis Type="Italic">Phyllodolon</Emphasis> in <Emphasis Type="Italic">Allium</Emphasis>     

Naoyuki Araki  Shin-Ichi Masuzaki  Hikaru Tsukazaki  Shigenori Yaguchi  Tadayuki Wako  Yosuke Tashiro  Naoki Yamauchi  Masayoshi Shigyo 《Euphytica》2010,173(3):321-328

The potential of microsatellite markers for use in genetic studies has been evaluated in Allium cultivated species (Allium cepa, A. fistulosum) and its allied species (A. altaicum, A. galanthum, A. roylei, A. vavilovii). A total of 77 polymerase chain reaction (PCR) primer pairs were employed, 76 of which amplified a single product or several products in either of the species. The 29 AMS primer pairs derived from A. cepa and 46 microsatellites primer pairs from A. fistulosum revealed a lot of polymorphic amplicons between seven Allium species. Some of the microsatellite markers were effective not only for identifying an intraspecific F₁ hybrid between shallot and bulb onion but also for applying to segregation analyses in its F₂ population. All of the microsatellite markers can be used for interspecific taxonomic analyses among two cultivated and four wild species of sections Cepa and Phyllodolon in Allium. Generally, our data support the results obtained from recently performed analyses using molecular and morphological markers. However, the phylogeny of A. roylei, a threatened species with several favorable genes, was still ambiguous due to its different positions in each dendrogram generated from the two primer sets originated from A. cepa and A. fistulosum.  相似文献   

Optimal sap flux sensor allocation for stand transpiration estimates: a non-dimensional analysis     

Hikaru Komatsu  Tomonori Kume  Yoshinori Shinohara 《Annals of Forest Science》2017,74(2):38

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Effect of accelerated aging treatment on a surface property and dynamic mechanical properties of commercial wood-based panels     

Sahriyanti Saad  Hikaru Kobori  Yoichi Kojima  Shigehiko Suzuki 《Journal of Wood Science》2017,63(5):496-505

Changes in surface property and mechanical properties in commercial particleboards and medium density fiberboards subjected to repetitive relative humidity treatment (i.e., aging treatment) were investigated. The stylus technique was used to evaluate surface roughness and a non-destructive vibrational test was used to evaluate dynamic bending strength during aging treatment. These methods evaluated the effect of aging treatment effectively. The aging treatment increased surface roughness and loss tangent (tan δ) values, but decreased dynamic modulus of elasticity (E _d) values of the panels compared to the respective initial values. The increment of surface roughness and E _d degradations observed were larger than the tan δ degradations. Surface roughness and tan δ seemed to be relatively dependent on resin type, whereas E _d was relatively independent under the aging treatment. Overall, the medium density fiberboard bonded methylene diphenyl diisocyanate (MDF(MDI)) panel was superior among the types of panels studied under the aging treatment. There was strong correlation between average roughness (R _a) increment and E _d retention.  相似文献   

Antifungal susceptibility of dermatophytes from racehorses in Japan     

Ryousuke Watanabe  Hikaru Huruta  Yuji Ueno  Toshio Nukada  Hidekazu Niwa  Naoki Shinyashiki  Rui Kano 《Veterinary dermatology》2021,32(5):474-e129

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