Oxidative stress inducers potentiate 2,3,7,8-tetrachlorodibenzo-p-dioxin-mediated pre-cardiac edema in larval zebrafish     

Katsuki TANAKA  Hikaru ADACHI  Hironobu AKASAKA  Junya TAMAOKI  Yuji FUSE  Makoto KOBAYASHI  Takio KITAZAWA  Hiroki TERAOKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1050

We reported the involvement of oxidative stress and prostaglandins including thromboxane and prostacyclin in pre-cardiac edema (early edema) caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). While the involvement of oxidative stress in TCDD-induced toxicity has been frequently reported, the mechanism of its action is still unclear. In the present study, oxidative stress inducers including paraquat, hydrogen peroxide (H₂O₂) and rotenone augmented early edema (edema) induced by a low concentration of TCDD (0.1 ppb) at 55 hr post fertilization (hpf), while each of them alone did not cause edema. Edema caused by TCDD plus oxidative stress inducers was almost abolished by antioxidants, an antagonist for thromboxane receptor (ICI-192,605) and an agonist for prostacyclin receptor (beraprost), suggesting that the site of action of these inducers was in the regular signaling pathway after activation of aryl hydrocarbon receptor type 2 (AHR2) by TCDD. Oxidative stress inducers also enhanced edema caused by an agonist for the thromboxane receptor (U46619), and the enhancement was also inhibited by antioxidants. Sulforaphane and auranofin, activators of Nrf2 that is a master regulator of anti-oxidative response, did not affect U46619-evoked edema but almost abolished TCDD-induced edema and potentiation by paraquat in both TCDD- and U46619-induced edema. Taken together, the results suggest that oxidative stress augments pre-cardiac edema caused by TCDD via activation of thromboxane receptor-mediated signaling in developing zebrafish. As paraquat and other oxidative stress inducers used also are environmental pollutants, interaction between dioxin-like compounds and exogenous source of oxidative stress should also be considered.  相似文献   

Detailed examination of the degradation of phenol derivatives under oxygen delignification conditions     

Yokoyama T  Matsumoto Y  Meshitsuka G 《Journal of agricultural and food chemistry》2007,55(4):1301-1307

Several phenolic compounds were subjected to oxygen-alkali oxidations under oxygen delignification conditions, and their degradations were examined in detail by applying a novel formula. The formula was established on the basis of the two following considerations. The degradation of the phenolic compounds should be expressed by the sum of two types, each of which is caused by molecular oxygen and by highly reactive active oxygen species (HAOS). The degradation should be described by a mathematical equation to which a rate function, k(t), dependent on reaction time t, is applied instead of a rate constant. By rearrangements, the following formula was obtained: k(t) = A/(t + B) (A, B are constants). This is hyperbola, and the illustration of k(t) visualized the contribution of HAOS to the degradation of the phenolic compounds. HAOS did not contribute so much to the degradation, especially at 70 degrees C, which suggests the low energy supply for the HAOS generation at 70 degrees C. The extrapolation of k(t) to the beginning of the reaction gives its initial value, k(initial), which is the rate constant of the reaction between the phenolic compounds and molecular oxygen. As expected, k(initial) was dependent on the electronic property of their substituents. Quantification of the phenolic compounds degraded by HAOS showed that the contribution of HAOS to the degradation is not great. The maximum contribution was observed in the oxidation of 2,6-dimethylphenol at 85 degrees C. In this case, 5 and 95% of the compound were degraded by HAOS and molecular oxygen, respectively.  相似文献   

Genetic and physical mapping of the partial resistance gene, pi34, to blast in rice   总被引：1，自引：0，他引：1  

Zenbayashi-Sawata K  Fukuoka S  Katagiri S  Fujisawa M  Matsumoto T  Ashizawa T  Koizumi S 《Phytopathology》2007,97(5):598-602

ABSTRACT Partial resistance to rice blast in the Oryza sativa japonica group cv. Chubu 32 is controlled by Pi34, a major quantitative trait locus (QTL) on chromosome 11, and several uncharacterized QTLs. The objectives of the study were (i) high-resolution genetic and physical mapping of Pi34 and (ii) identification of new QTL imparting resistance to rice blast. Chubu 32 was crossed with a susceptible chromosomal segment substitution line (CSSL) of cv. Koshihikari. From 4,012 of segregating individuals, 213 recombinants in the Pi34 region were screened by using polymerase chain reaction-based markers and tested resistance in the field and greenhouse. The Pi34 locus is located in the 54.1-kb region on the genomic sequence of cv. Nipponbare. We constructed a bacterial artificial chromosome (BAC) library of Chubu 32, selected the clone containing Pi34, and sequenced it. The Pi34 locus consequently was located on an interval of 65.3 kb containing 10 predicted open reading frames (ORFs). Two of these ORFs were predicted only in Chubu 32 and encoded transposable elements. The other eight ORFs were found in both Chubu 32 and Nipponbare and one of them, which encoded an unknown protein, showed significantly different amino acid sequences between two cultivars. The new QTL, Piq6(t), was detected on the short arm of chromosome 6 and the genetic distance of flanking markers was 16.9 centimorgans in Nipponbare. Pi34 and Piq6(t) acted additively on resistance to rice blast but the effect of Piq6(t) was relatively small compared with Pi34.  相似文献   

Rearing of squid hatchlings, Heterololigo bleekeri (Keferstein 1866) up to 2 months in a closed seawater system     

Yuzuru Ikeda  Ikuko Sakurazawa  Kingo Ito  Yasunori Sakurai  & Gen Matsumoto 《Aquaculture Research》2005,36(4):409-412

  相似文献   

Difference Between Tetrodotoxin and Saxitoxins in Accumulation in Puffer Fish Takifugu rubripes Liver Tissue Slices     

T. Matsumoto  Y. Nagashima  K. Takayama  K. Shimakura  K. Shiomi 《Fish physiology and biochemistry》2005,31(1):95-100

In vitro accumulation of tetrodotoxin (TTX) and paralytic shellfish toxin (PST) in tiger puffer fish Takifugu rubripes was investigated using liver tissue slices. When T. rubripes liver slices were incubated with Leibovitz’s L-15 medium containing 0.13 mM TTX at 20 °C in air with saturated humidity, they accumulated 21.5 ± 7.3 μg TTX g^?1 liver after the incubation for 12 h and increased to 55.3 ± 8.2 μg TTX g^?1 liver at 48 h. In the incubation of T. rubripes liver slices with 0.13 mM PST-containing medium, PST was detected 6.3 ± 0.9 μg g^?1 liver at 12 h and reached a plateau thereafter. These results reveal the difference between TTX and PST in accumulation in T. rubripes liver tissue slices. To examine the variation in PST accumulation among fish species, the liver tissue slices from tiger puffer fish T. rubripes, parrot-bass Oplegnathus fasciatus and green ling Hexagrammos otakii were incubated at a concentration of 0.027 mM PST. The toxin contents of 3.0 μg g^?1 liver were observed at 8 h regardless of fish species but were not increased subsequently, showing no variety among these three species as to accumulation patterns of PST. It is noted that the tiger puffer fish T. rubripes liver specifically accumulate TTX in preference to PST.  相似文献   

Restriction fragment length polymorphism for the Yc subunit gene of rat liver glutathione S-transferase     

Y Sasaki  M Hayashi  K Matsumoto  S Namioka 《The Japanese journal of veterinary research》1990,38(2):35-42

An altered expression of the Yc subunit gene of rat glutathione S-transferase (GST) in the liver of the LEC rat, which is a mutant strain with spontaneous hereditary hepatitis associated with severe jaundice, has been reported. To provide further information concerning the structure of the Yc subunit gene, we carried out the Southern blot hybridization analysis of DNA samples from rats of eight different inbred strains including LEC with cDNA complementary to mRNA specific for the Yc subunit of rat liver GST as a probe. The hybridization patterns of the DNA samples from rats belonging to the different inbred strains showed interstrain variation in the length of restriction fragments with four restriction endonucleases. Since the DNA samples prepared from several rats of one inbred strain gave an identical hybridization pattern, the restriction fragment patterns for the Yc gene could be used as markers for genetic monitoring of inbred rat strains. Although the altered expression of Yc-Yc activity of GST has been observed in the liver of the LEC rat, the characteristic changes in the gene structure of the Yc subunit of LEC rat were not detected in the present hybridization analysis.  相似文献   

Genetic mapping of AFLP markers in Japanese bunching onion (<Emphasis Type="Italic">Allium fistulosum</Emphasis>)     

Takayoshi?OharaEmail author  Yeon-Sang?Song  Hikaru?Tsukazaki  Tadayuki?Wako  Tsukasa?Nunome  Akio?Kojima 《Euphytica》2005,144(3):255-263

Summary The first genetic linkage map of Japanese bunching onion (Allium fistulosum) based primarily on AFLP markers was constructed using reciprocally backcrossed progenies. They were 120 plants each of (P₁)BC₁ and (P₂)BC₁ populations derived from a cross between single plants of two inbred lines: D1s-15s-22 (P₁) and J1s-14s-20 (P₂). Based on the (P₂)BC₁ population, a linkage map of P₁ was constructed. It comprises 164 markers – 149 amplified fragment length polymorphisms (AFLPs), 2 cleaved amplified polymorphic sequences (CAPSs), and 12 simple sequence repeats (SSRs) from Japanese bunching onion, and 1 SSR from bulb onion (A. cepa) – on 15 linkage groups covering 947 centiMorgans (cM). The linkage map of P₂ was constructed with the (P₁)BC₁ population and composed of 120 loci – 105 AFLPs, 1 CAPS, and 13 SSRs developed from Japanese bunching onion and 1 SSR from bulb onion – on 14 linkage groups covering 775 cM. Both maps were not saturated but were considered to cover the majority of the genome. Nine linkage groups in P₂ map were connected with their counterparts in P₁ map using co-dominant anchor markers, 13 SSRs and 1 CAPS.  相似文献   

Revisiting the mechanism of <Emphasis Type="Italic">β-O</Emphasis>-4 bond cleavage during acidolysis of lignin IV: dependence of acidolysis reaction on the type of acid     

Takaaki Imai  Tomoya Yokoyama  Yuji Matsumoto 《Journal of Wood Science》2011,57(3):219-225

The dependence of the acidolysis reaction of a C₆-C₃ dimeric nonphenolic β-O-4 type lignin model compound, 2-(2-methoxyphenoxy)-1-(3,4-dimethoxyphenyl) propane-1,3-diol (veratrylglycerol-β-guaiacyl ether, VG), on the type of acid applied was examined using three different acids [0.2 mol/l HCl, 0.2 mol/l HBr, and 0.1 mol/l (0.2 N) H₂SO₄ in 82% aqueous 1,4-dioxane at 85°C]. In the HCl system, the major reaction modes of the corresponding benzyl cation-type intermediate (BC), which is produced by protonation of the α-hydroxyl group of VG and successive release of the water molecule, are the abstraction of the β-proton and hydride transfer from the β-to the α-position. The liberation of formaldehyde from the γ-hydroxymethyl group of BC is the predominant reaction mode in the H₂SO₄ system. Apparently, an unknown reaction mode or modes is operative in the early stage of the HBr system that causes rapid disappearance of VG accompanied by the quantitative formation of 2-methoxyphenol without affording the common counterpart of a Hibbert’s ketone, 1-hydroxy-3-(3,4-dimethoxyphenyl) propan-2-one. The reaction mode in the HBr system changes with the progress of the reaction and is the same as that in the HCl system after the early stage.  相似文献   

Effects of Inoculation of Phototrophic Purple Bacteria on Grain Yield of Rice and Nitrogenase Activity of Paddy Soil in a Pot Experiment     

Naoki Harada  Masaya Nishiyama  Shigeto Otsuka  Satoshi Matsumoto 《Soil Science and Plant Nutrition》2005,51(3):361-367

Phototrophic purple bacteria (PPB) are one group of dinitrogen (N₂)-fixing bacteria often found in the anoxic and photic zone of paddy soil. In the present study, the effects of inoculation of PPB on grain yield of rice and changes in their populations and nitrogenase activity were investigated with and without surface application of rice straw in a pot experiment. As an inoculant, Rhodopseudomonas palustris strain KN122 isolated from a paddy soil was selected, and the cell suspension was inoculated into the floodwater once or three times during the cultivation. As a result, the inoculation of PPB increased the grain yield of rice. Compared to the control, the grain yield was 9% higher in the inoculated plot without rice straw application. The treatment was more effective in combination with rice straw application. In the plots where PPB were inoculated with rice straw, grain yields were 21% (single inoculation) and 29% (triple inoculation) higher. Populations of PPB in the plots without rice straw application significantly increased by the inoculation, unlike those in the plots with rice straw application, except for the bulk soil. Phototrophic and heterotrophic nitrogenase activities (acetylene-reducing activity) associated with the soils or residues of the rice straw were not affected by PPB inoculation throughout the experiment. This indicates that the inoculation of PPB into floodwater may not be effective for enhancing N₂ fixation in paddy soils and that the beneficial effect of PPB inoculation on the grain yield of rice may be due to unidentified functions of PPB other than biological N₂ fixation.  相似文献   

The effect of alkaline dust decline on the precipitation chemistry in northern Japan     

I. Noguchi  T. Kato  M. Akiyama  H. Otsuka  Y. Matsumoto 《Water, air, and soil pollution》1995,85(4):2357-2362

The precipitation chemistry in northern Japan, especially Hokkaido, has been investigated since 1982. This area has often been found to have high concentrations of alkaline road dust (asphalt dust) in the air, caused by the use of studded tires during the winter. It is well known that the composition of precipitation in these areas is often dominated by asphalt dust including calcium bicarbonate. However, recently the concentration of asphalt dust in the air has decreased owing to a ban on the use of studded tires. Simultaneously, in precipitation, the lowering of pH values and the increase of hydrogen ion depositions have been occurring owing to the decrease of non-sea-salt calcium ions (nss-Ca²⁺) concentrations and depositions derived from asphalt dust. In addition, we found that a decrease of nss-Ca²⁺ firstly leads to a decrease of bicarbonate ions (HCO₃ ^?), the counter ion to nss-Ca²⁺ in asphalt dust. Therefore, the increase of H⁺ concentrations and depositions was great in comparison with the decrease of nss-Ca²⁺ concentrations and depositions in areas where the HCO₃ ^?concentrations, varied by pH, and depositions had been low. Furthermore, this variation was mainly observed in the ionic composition of snow cover and snowfall at sites along the Japan Sea in northern Japan during winter. In this area, the Acid Shock effect may become a serious problem from the decline of pH values in melting snow. Moreover, we found that ammonium ions and non-sea-salt sulfate ions depositions have also been decreasing in response to a decrease of nss-Ca²⁺ depositions, derived from asphalt dust. It seems that this phenomenon is caused by the decrease of asphalt dust concentrations in the air.  相似文献