Changes in volatile compounds of living Pacific oyster Crassostrea gigas during air-exposed storage     

Kawabe  Shinya  Murakami  Haruna  Usui  Masakatsu  Miyasaki  Taiko 《Fisheries Science》2019,85(4):747-755

Fisheries Science - The changes in volatile compounds of living oysters Crassostrea gigas with shells under air-exposed storage for 7 days at 5 and 20 °C were investigated...  相似文献   

Expressed sequence tag analysis of phyllosomas and hemocytes of Japanese spiny lobster Panulirus japonicus     

Duangjai Pisuttharachai  Motoshige Yasuike  Hideaki Aono  Keisuke Murakami  Hidehiro Kondo  Takashi Aoki  Ikuo Hirono 《Fisheries Science》2009,75(1):195-206

Two complementary DNA (cDNA) libraries were constructed from phyllosomas and hemocytes of adult Japanese spiny lobster Panulirus japonicus and a total of 2,673 expressed sequence tags (ESTs) were obtained. After assembly and clustering, 450 and 458 unique sequences were found from the phyllosoma and hemocyte cDNA libraries, respectively. Of these, 114 and 220 ESTs showed significant homologies with known genes in the National Centre for Biotechnology Information (NCBI) database. The remaining sequences were of unknown function. Immune-related genes found in this study include lectin, proteinase inhibitor, prophenoloxidase, heat-shock protein, antimicrobial peptide, and a few putative defense-related proteins.  相似文献   

Determination of the spawning history of Pacific saury <Emphasis Type="Italic">Cololabis saira</Emphasis> from rearing experiments: identification of post-spawning fish from histological observations of ovarian arterioles     

Satoshi Suyama  Akio Shimizu  Sayoko Isu  Hitomi Ozawa  Taizo Morioka  Mitsuhiro Nakaya  Toru Nakagawa  Naoto Murakami  Takashi Ichikawa  Yasuhiro Ueno 《Fisheries Science》2016,82(3):445-457

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Microperforated leaf blotting on polyvinylidene difluoride and nylon membranes to analyze spatial distribution of endogenous and viral gene expression in plant leaves     

Taiki Murakami  Ryou Tayama  Kenji S. Nakahara 《Journal of General Plant Pathology》2016,82(5):254-260

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Rhizoctonia blight of turnip green caused by Rhizoctonia solani AG-4 HG-III     

Hiroyuki Sekiguchi  Takeshi Toda  Erika Sato  Keisuke Tomioka  Hiroharu Murakami  Ryota Amemiya  Naoyuki Hirako  Masato Nakata  Mitsuro Hyakumachi  Seiya Tsushima 《Journal of General Plant Pathology》2015,81(6):454-456

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A study on activated carbon nanofibrous adsorbents prepared by technology for electrospun composite yarn     

Jiunn-Yih Lee  Ching-Hsiang Hsu  Ching-Iuan Su  Ri-Ichi Murakami  Chi-Wei Lin  Ching-Hsiang Lu 《Fibers and Polymers》2015,16(11):2437-2444

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Expression of vascular endothelial growth factor, basic fibroblast growth factor, and their receptors (flt-1, flk-1, and flg-1) in canine vascular tumors     

Yonemaru K  Sakai H  Murakami M  Yanai T  Masegi T 《Veterinary pathology》2006,43(6):971-980

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Autoclaved spent substrate of hatakeshimeji mushroom (Lyophyllum decastes Sing.) and its water extract protect cucumber from anthracnose     

R.Y. ParadaS. Murakami  N. ShimomuraM. Egusa  H. Otani 《Crop Protection》2011,30(4):443-450

The protective effect of fresh spent mushroom substrate (SMS) of hatakeshimeji (Lyophyllum decastes Sing.), a popular culinary-medicinal mushroom, and its water extract against anthracnose of cucumber was investigated. Plants were treated with water extract from SMS or autoclaved water extract by spraying the whole plant or by dipping the first true leaf, and inoculated with Colletotrichum orbiculare seven days later. Plants treated with either of the extracts showed a significant reduction of necrotic lesions. On the other hand, when plants were grown in a mixture (1:2, v/v) of SMS or autoclaved SMS and soil, a disease reduction of over 70% was observed in autoclaved SMS. The water extract showed no antifungal activity against spore germination and mycelial growth of the pathogen. Real-time PCR analyses of chitinase and β-1,3-glucanase genes revealed a significant increase of expressions after 24 h of pathogen inoculation in water extract-treated plants compared with the control plants. These results suggest that water-soluble and heat-stable compounds in SMS enhance the state of systemic acquired resistance and protect cucumbers from anthracnose. Thus, the use of SMS for disease control may offer a new technology for the recycling and management of waste from mushroom cultivation.  相似文献   

Effects of β‐carotene‐enriched dry carrots on β‐carotene status and colostral immunoglobulin in β‐carotene‐deficient Japanese Black cows          下载免费PDF全文

Yoshitaka Nishijima  Saya Taniguchi  Shuntaro Ikeda  Kenichi Yoshitani  Takashi Hamano  Hiroshi Tani  Masahiko Fujita  Kenji Murakami  Keisuke Kogusa  Kenji Sato  Miki Sugimoto  Shinichi Kume 《Animal Science Journal》2017,88(4):653-658

Data from 18 β‐carotene‐deficient Japanese Black cows were collected to clarify the effects of feeding β‐carotene‐enriched dry carrots on β‐carotene status and colostral immunoglobulin (Ig) in cows. Cows were assigned to control or carrot groups from 3 weeks before the expected calving date to parturition, and supplemental β‐carotene from dry carrots was 138 mg/day in the carrot group. Plasma β‐carotene concentrations in the control and carrot groups at parturition were 95 and 120 μg/dL, and feeding dry carrots slightly improved plasma β‐carotene at parturition. Feeding dry carrots increased colostral IgA concentrations in cows and tended to increase colostral IgG₁, but colostral IgM, IgG₂, β‐carotene and vitamin A were not affected by the treatment. Feeding dry carrots had no effects on plasma IgG₁, IgA and IgM concentrations in cows, but plasma IgG₁ concentrations decreased rapidly from 3 weeks before the expected calving date to parturition. These results indicate that feeding β‐carotene‐enriched dry carrots is effective to enhance colostral IgA and IgG₁ concentrations in β‐carotene‐deficient cows.  相似文献   

Effect of high molecular weight carbohydrates on bud cell formation by <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> in potato dextrose broth     

Kenji Yokota  Takuma Teraoka  Yoshimasa Tsujii  Hirofumi Suzuki  Keiichi Murakami  Eitaro Miwa  Kyoko Higuchi 《Journal of General Plant Pathology》2010,76(3):219-224

Potato dextrose broth (PDB), one of the most popular culture media for fungi, can be made in the laboratory from potato extract and glucose (handmade PDB) and also bought as powder (commercial PDB). We compared growth of Fusarium oxysporum in handmade and commercial PDBs. For seven strains tested, bud cell formation and fungal biomass were higher in handmade PDB than in commercial PDB. Gel filtration analyses revealed that handmade PDB contained carbohydrates with molecular weights >20 MDa, while most carbohydrates in commercial PDB were <45 kDa. Freshly prepared, raw potato starch had a molecular weight >20 MDa and enhanced bud cell formation by five of seven strains when added to commercial PDB. The addition of glucose to commercial PDB promoted biomass production but not bud cell formation. Furthermore, in a semisynthetic medium containing raw potato starch, bud cell formation was enhanced for all strains tested. These results indicate that the high molecular carbohydrates present in raw potatoes enhanced bud cell formation by Fusarium oxysporum.  相似文献