Isolation and identification of boron-accumulating bacteria from contaminated soils and active sludge     

Hiroki MIWA  Toru FUJIWARA 《Soil Science and Plant Nutrition》2009,55(5):643-646

High levels of boron are toxic to living organisms. The removal of boron from contaminated water is an important way of dealing with this environmental problem. For this purpose, we screened for bacteria that can absorb high levels of toxic boron from the environment. A range of boron-accumulating bacteria was isolated from boron-contaminated soil in Turkey, from uncontaminated soil in Tokyo, Japan, and from active sludge in Tokyo by semi-quantifying the boron accumulation of each single-cultured bacterium. Intracellular boron concentrations ranged from 0.3 to 1.36 nmol g⁻¹ dry weight. Nineteen isolates identified by 16S rRNA gene sequence analysis were most closely related to the genera Bacillus , Variovorax , Pseudomonas , Shewanella , Mycobacterium and Rhodococcus . To our knowledge, this is the first study examining a wide range of bacterial strains in terms of boron accumulation.  相似文献   

Dietary conjugated linoleic acid lowered tumor necrosis factor-alpha content and altered expression of genes related to lipid metabolism and insulin sensitivity in the skeletal muscle of Zucker rats     

Inoue N  Nagao K  Wang YM  Noguchi H  Shirouchi B  Yanagita T 《Journal of agricultural and food chemistry》2006,54(20):7935-7939

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Genomic survey of polymorphisms in pattern recognition receptors and their possible relationship to infections in pigs     

Uenishi H  Shinkai H  Morozumi T  Muneta Y 《Veterinary immunology and immunopathology》2012,148(1-2):69-73

Recent progress in the accumulation of pig genomic information has enabled us to comprehensively explore polymorphisms in pig genes. One of our targets for exploration has been the genes encoding molecules related to pathogen recognition, such as pattern recognition receptors (PRRs). PRRs play a role in the innate immune system, and possess various members such as Toll-like receptors (TLRs), NOD-like receptors (NLRs), RIG-like helicases (RLHs), and C-type lectin-like receptors (CLRs). PRRs are required for the monitoring of pathogens; therefore, polymorphisms in PRRs may influence molecular functions such as ligand recognition. There have been many studies on the relationship between polymorphisms within PRR genes and disease susceptibility in humans and mice. Our studies have revealed that porcine PRR genes possess many nonsynonymous polymorphisms, particularly in regions encoding the ectodomains of TLRs localized on the cell surface. The genes encoding TLRs located on the membrane of intracellular compartments, and cytoplasmic PRRs such as NLRs and RLHs, also possessed nonsynonymous polymorphisms. Several observations indicate that there are relationships between polymorphisms in PRR or related genes and disease susceptibility in livestock animals including pig. Such information may contribute to breeding aimed at disease resistance, and effective vaccine design.  相似文献   

Apple necrotic mosaic virus,a novel ilarvirus from mosaic-diseased apple trees in Japan and China     

Hiroki Noda  Noriko Yamagishi  Hajime Yaegashi  Fei Xing  Jipeng Xie  Shifang Li  Tao Zhou  Tsutae Ito  Nobuyuki Yoshikawa 《Journal of General Plant Pathology》2017,83(2):83-90

The causal agent of apple mosaic disease has been previously thought to be solely caused by apple mosaic virus (ApMV). In this study, we report that a novel ilarvirus is also associated with apple mosaic disease. Next-generation sequencing analysis of an apple tree showing mosaic symptoms revealed that the tree was infected with three apple latent viruses (apple stem pitting virus, apple stem grooving virus, and apple chlorotic leaf spot virus) and a novel ilarvirus (given the name apple necrotic mosaic virus (ApNMV)) that is closely related to Prunus necrotic ringspot virus (PNRSV) and ApMV. The genome of ApNMV consists of RNA1 (3378 nt), RNA2 (2767 nt), and RNA3 (1956 nt). A phylogenetic analysis based on the coat protein amino acid sequences indicated that the novel virus belongs to the same subgroup 3 of the genus Ilarvirus as PNRSV and ApMV. The presence of mosaic leaves, which tend to be unevenly distributed in diseased apple trees, was correlated with the internal distribution of ApNMV. RT-PCR detection of mosaic-diseased apple trees in Japan indicated that ApNMV was detected in apple trees introduced from China, whereas ApMV was detected from cultivated apple trees in domestic orchards. Consistent with these findings, a survey of mosaic-diseased apple trees in major apple-producing provinces in China revealed that the majority of apple trees showing mosaic symptoms in China are infected with ApNMV.  相似文献   

Continuous nondestructive monitoring of larval feeding activity and development of the bamboo powderpost beetle <Emphasis Type="Italic">Dinoderus minutus</Emphasis> using acoustic emission     

Hiroki Watanabe  Yoshiyuki Yanase  Yoshihisa Fujii 《Journal of Wood Science》2018,64(2):138-148

The life history and feeding biology of the bamboo powderpost beetle Dinoderus minutus remain poorly understood because the beetles’ oviposition, development, and feeding take place inside bamboo culms. In this study, acoustic emission (AE) monitoring was applied for continuous nondestructive analysis of larval feeding activity and development from the first instar to adult eclosion. Newly hatched larvae were inoculated individually into pieces of madake (Phyllostachys bambusoides) culms. AE hits were recorded using single AE sensors fixed onto the bamboo pieces. Generation of AE hits indicated that larval feeding activity had begun after inoculation. Based on the time course of the hourly AE hit rate, the larvae were feeding constantly during each instar, and feeding activity only ceased during periods of ecdysis and pupation. Half of the individuals examined underwent seven instars and the other half underwent eight instars. The time course of AE hit rate per 5 min exhibited periodic cycles, where continuous meals were separated by inactive phases of ca. 5 min, with an average dominant period of each instar ranging between 0.76 and 2.19 h. After correcting the AE data based on distance attenuation of AE waves, the tendency that AE amplitude increased as the larvae developed through ecdysis events became more apparent. AE monitoring continued after adult eclosion, and the feeding activity of the newly emerged adults continued almost ceaselessly during the Reifungsfrass period.  相似文献   

Bite Wounds Caused by a Wild Boar: A Case Report     

Hiroki Nagasawa  Kazuhiko Omori  Hiroyuki Maeda  Ikuto Takeuchi  Suguru Kato  Takashi Iso  Kei Jitsuiki  Toshihiko Yoshizawa  Kouhei Ishikawa  Hiromichi Ohsaka  Youichi Yanagawa 《The Forestry Chronicle》2017,28(4):313-317

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Hypofractionated radiation therapy in the treatment of canine thymoma: Retrospective study of eight cases          下载免费PDF全文

Sho Goto  Mami Murakami  Mifumi Kawabe  Ryota Iwasaki  Kazuki Heishima  Hiroki Sakai  Takashi Mori 《Veterinary radiology & ultrasound》2017,58(5):613-620

Thymomas are one of the most common tumors of the cranial mediastinum in dogs; however there is limited information available on the use of radiation therapy for treating this neoplasm. Objectives of the current retrospective observational study were to describe outcomes and side effects of a hypofractionated radiation therapy protocol in a group of dogs with confirmed thymoma. A total of eight dogs were included. To generate individualized treatment plans, we designed the planning target volume according to the limits on mean lung dose and the percentage of the total lung volume exceeding 20 Gy (V20). The total administered dose was 48–49 Gy, with one fraction per week for a total of six to seven fractions. After therapy, two dogs achieved complete responses, two achieved partial responses, and the disease remained stable in two. Two dogs died during the radiation therapy protocol and were not classified. The median mean lung dose and V20 were 6.0 Gy (range: 3.1–15.0 Gy) and 12.4% (range: 2.3–27.5%), respectively. The overall response rate was 50.0%, and the median time to response following treatment initiation was 22 days (range: 14–115 days). Acute and late side effects were common in the skin and/or lung and were self‐limiting or asymptomatic. The median survival time was not reached (range: 8–1128 days) and the 1 year survival rate was 75.0%. Hypofractionated radiation therapy was well tolerated in this sample of dogs with thymoma and may be considered when owners decline surgical treatment or the tumor is deemed unresectable.  相似文献   

Effect of dietary kapok oil supplementation on growth performance,carcass traits,meat quality and sensory traits of pork in finishing‐pigs          下载免费PDF全文

Keisuke Maeda  Kimiko Kohira  Hiroki Kubota  Kousuke Yamanaka  Kaoru Saito  Masakazu Irie 《Animal Science Journal》2017,88(8):1066-1074

Kapok seed and oil from the tropical zone are widely used as pig feed to harden porcine fat in Japan. This study evaluated the effect of dietary kapok oil supplementation on pork quality and sensory traits. Five Duroc pigs each were assigned to an experimental group supplemented with kapok oil and a control group. Dietary kapok oil supplementation had no effect on growth performance and intramuscular fat content in the Longissimus dorsi muscle (LM). Supplemental kapok oil increased saturated fatty acid contents in subcutaneous and intramuscular fat and decreased monounsaturated fatty acid levels (P < 0.05). Off‐flavor detection by a trained panel was higher in the experimental than the control group (P < 0.05), but tenderness, juiciness, texture and flavor intensity of LM chops were similar in both groups. The overall palatability of pork as judged by a consumer panel decreased with kapok oil supplementation (P < 0.01). These results indicate that while growth performance, intramuscular fat contents and carcass characteristics were unchanged, while dietary kapok oil supplementation makes firm fat to prevent inferior soft fat in pork, it can lower the palatability of pork due to a decrease in monounsaturated fatty acids.  相似文献   

Bovine Ocular Squamous Cell Carcinoma     

Hiroki TsujitaCaryn E. Plummer  DVM 《Veterinary Clinics of North America: Food Animal Practice》2010

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Plasticity of animal genome architecture unmasked by rapid evolution of a pelagic tunicate     

Denoeud F  Henriet S  Mungpakdee S  Aury JM  Da Silva C  Brinkmann H  Mikhaleva J  Olsen LC  Jubin C  Cañestro C  Bouquet JM  Danks G  Poulain J  Campsteijn C  Adamski M  Cross I  Yadetie F  Muffato M  Louis A  Butcher S  Tsagkogeorga G  Konrad A  Singh S  Jensen MF  Cong EH  Eikeseth-Otteraa H  Noel B  Anthouard V  Porcel BM  Kachouri-Lafond R  Nishino A  Ugolini M  Chourrout P  Nishida H  Aasland R  Huzurbazar S  Westhof E  Delsuc F  Lehrach H  Reinhardt R  Weissenbach J  Roy SW  Artiguenave F  Postlethwait JH  Manak JR  Thompson EM 《Science (New York, N.Y.)》2010,330(6009):1381-1385

Genomes of animals as different as sponges and humans show conservation of global architecture. Here we show that multiple genomic features including transposon diversity, developmental gene repertoire, physical gene order, and intron-exon organization are shattered in the tunicate Oikopleura, belonging to the sister group of vertebrates and retaining chordate morphology. Ancestral architecture of animal genomes can be deeply modified and may therefore be largely nonadaptive. This rapidly evolving animal lineage thus offers unique perspectives on the level of genome plasticity. It also illuminates issues as fundamental as the mechanisms of intron gain.  相似文献