VASA (DDX4) is a Putative Marker for Spermatogonia,Spermatocytes and Round Spermatids in Stallions          下载免费PDF全文

JY Kim  HJ Jung  MJ Yoon 《Reproduction in domestic animals》2015,50(6):1032-1038

Expression of the protein DDX4/MVH, or VASA, has been reported in germ cells of several species. The main objectives of this study were to (i) investigate VASA expression patterns in testicular cells of stallions at two different reproductive stages (pre‐pubertal and post‐pubertal) and (ii) evaluate the use of VASA antibody as a molecular marker for single germ cells from stallions. Testicular tissues were obtained from stallions and categorized as pre‐pubertal and post‐pubertal based on the formation of lumen and status of spermatogenesis on the cross section of seminiferous tubules. The results of Western blot showed a VASA protein band located at 76 kDa, indicating that the rabbit antibody has a cross‐reactivity with horse testicular tissues. The result of immunolabelling showed that VASA was expressed in the cytoplasm of spermatogonia at both reproductive stages and in spermatocytes and round spermatids at the post‐pubertal stage. GATA4‐positive Sertoli cells and Leydig cells located in the interstitial space were not immunolabelled with VASA. These results suggest that VASA can be utilized as a molecular marker for germ cells of stallions at pre‐pubertal and post‐pubertal stages. Interestingly, immunolabelling intensity was significantly higher in pachytene spermatocytes compared to spermatogonia and round spermatid. VASA antibody was also effective for staining of single germ cell preparations. In conclusion, VASA protein expression can be used as a marker for identification of spermatogonia, spermatocytes and round spermatids in testicular tissues of stallions.  相似文献   

Canine osteosarcoma cell lines from patients with differing serum alkaline phosphatase concentrations display no behavioural differences in vitro          下载免费PDF全文

K. E. Holmes  V. Thompson  C. M. Piskun  R. A. Kohnken  M. K. Huelsmeyer  T. M. Fan  T. J. Stein 《Veterinary and comparative oncology》2015,13(3):166-175

Osteosarcoma is an aggressive malignancy and represents the most frequent primary bone malignancy of dogs and humans. Prognostic factors reported for osteosarcoma include tumour size, presence of metastatic disease and serum alkaline phosphatase (ALP) concentration at the time of diagnosis. To date, there have been no studies to determine whether the behaviour of osteosarcoma cells differ based on serum ALP concentration. Here, we report on the generation of six canine osteosarcoma cell lines from osteosarcoma‐bearing dogs with differences in serum ALP concentration. To determine whether in vitro behaviour differs between primary osteosarcoma cell lines generated from patients with normal or increased serum ALP, assays were performed to evaluate proliferation, migration, invasion and chemosensitivity. There were no significant differences in cell proliferation, migration, invasion or chemosensitivity between cell lines associated with normal or increased serum ALP concentration.  相似文献   

THE NINETEENTH INTERNATIONAL CONGRESS OF AMERICANISTS     

Holmes WA  Hrdliccaronka A 《Science (New York, N.Y.)》1915,42(1073):116

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不同消毒剂及清洗温度对番茄货架期腐烂率的影响   总被引：2，自引：0，他引：2  

王金玉  张学杰  郭科  孙雅君  Robert Holmes  Simone Kreidl 《中国蔬菜》2009,1(12):7-10

针对番茄采后腐烂率较高的问题,研究了不同清洗温度〔果温（28±5）℃〕下消毒剂（100  mg·kg^-1 NaClO和5 mg·kg^-1 ClO₂）和自来水清洗处理对番茄货架期（PE包装,室温28～30 ℃,7 d）腐 烂率的影响。结果表明,与不清洗对照相比,清洗后番茄果实腐烂率由47 %下降到9 %～26 %;清洗温度 对自来水和ClO₂处理的番茄果实腐烂率影响不大,但高于果温（28 ℃）5 ℃的NaClO处理的番茄果实腐 烂率较低于果温5 ℃的NaClO处理显著降低,其货架7 d番茄果实腐烂率仅为9 %,是各清洗处理中效果最 好的。  相似文献   

Soil and landform features of mountainous terrain in South Thailand     

K.J. Virgo  D.A. Holmes 《Geoderma》1977,18(3):207-225

The results of reconnaissance surveys over 2,000 Km² are used to described mountainous terrain on granite, gneiss and pelite. Detailed morphological and laboratory analytical data are presented for soils derived from each of these rock types. The soils, which are classed as Paleudults, are acid, kaolinite-rich and deficient in nutrients. Soils formed on granite and gneiss have high proportions of coarse quartz particles (2.0–50.0 mm); the dilution effect of this inert material further reduces their effective nutrient status. The degree of weathering, as indicated by the silt:silt+clay ratio, increases with profile depth.Mean gradients recorded on the granite and pelite hillslopes are 27° and 29°, respectively. A greater uniformity of slopes was observed on pelites than on granites; the ‘missing angle’ technique was employed to identify ‘natural’ slope classes. Gradients tend to increase downlopw, indicating a rapid removal of erosion products at the footslope and a rejuvenation of the valleys. A significant association was recorded between soil depth and slope gradient on pelite but not on granite or gneiss landforms.  相似文献   

THE EFFECT OF NITROGEN APPLICATION TO PASTURE ON BEEF PRODUCTION     

J. C. Holmes  R. W. Lang 《Grass and Forage Science》1974,29(2):121-131

Five grazing experiments each lasting 2 or 3 years were made between 1955 and 1967, all starting in the first year of ryegrass/cocksfoot/clover or ryegrass/clover leys. A high and a low rate of N, 235 and 45 Ib/ac on average (263 and 51 kg/ha) were compared for beef production. High- and low-N treatments gave mean clover contents for the grazing season of 8 and 24 % on a dry-weight basis, respectively. High N consistently gave a smaller liveweight gain/animal than low N, on average 1±92 and 2±08 Ib/day (0±87 and 0±94 kg/day), respectively. Liveweight gain/ac was 20% greater for high N than for low N, and in terms of net energy the production from high- and low-N, respectively, was 18,500 and 15,000 MJ/ac (45,700 and 37,100 MJ/ha). Data from these experiments, together with published results, were used to calculate a regression of liveweight gain response on N rate and an equation was derived from this to express the output in terms of profit. At 1971 prices profit was maximal at λ0±9/ac (λ2/ha) with 112 Ib N/ac (125 kg N/ha); it was considerably greater at 1973 prices when higher rates of N were justified.  相似文献   

THE EFFECT OF NITROGEN AND STOCKING RATE ON THE OUTPUT OF PASTURE GRAZED BY BEEF CATTLE     

I. M. Yiakoumettis  W. Holmes 《Grass and Forage Science》1972,27(3):183-192

An experiment was conducted in two successire years to measure the effect of two levels of fertilizer N, 50 and 300 kg/ha (45 and 270 Ib/ac) on the productivity of pastures grazed by young beef cattle. Two stocking rates were imposed at the lower N level and 4 at the higher level. The responses per kg fertilizer N were approximately 1 kg liveweight gain, 20–24 Meal ME and 8–9 kg DM. Maximal yields of about 1000 kg gain/ha (890 Ib/ac) and 19,000 Meal ME/ha (7700 Mcal ME/acre) were recorded. Animal performance was similar on the low and the high N pastures. There was evidence that the chemical quality of pasture was lower on the low N pasture in the first year, but there was no difference in the second year. The numbers of dung pats per ha and the refusal of herbage due to fouling were reduced by Increasing the stocking rate.  相似文献   

Book review: Aquatic plants in Britain and Ireland,C.D. Preston and J.M. Croft,Harley Books,Colchester, UK, 1997, 368 pp. Price £25.00. ISBN 0 946589 550 (hb only).     

Nigel Holmes 《水产资源保护:海洋与淡水生态系统》1997,7(4):330-330

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Biological and molecular characterization of Potato yellow blotch virus,a new species of the genus Potyvirus     

C. Nisbet  W. A. Monger  S. Ross  R. F. Holmes  Y. Nova  C. Thomson  H. A. Goodfellow  C. Lacomme  C. J. Jeffries 《Plant pathology》2019,68(2):251-260

A new species of the genus Potyvirus infecting potatoes, with the proposed name Potato yellow blotch virus (PYBV), was discovered in a breeding line 99m-022-026 in Scotland. The infected plants show isolated yellow blotches on the leaves. The genome of PYBV contains a large open reading frame encoding a single polyprotein of 3054 amino acids. Sequence analysis shows that PYBV is closely related to Potato virus A (PVA), with an overall 72% identity at the nucleotide level for the whole genome. The least conserved P1 protease gene shares only 50% nucleotide identity with PVA. The host range of PYBV was comparable to PVA on solanaceaous and non-solanaceous indicator plant species with the exception of Solanum demissum A and Y. Different symptoms were also observed for PYBV and PVA in Nicotiana benthamiana, Nicotiana hesperis and Nicotiana occidentalis P1. The susceptibility of potato (Solanum tuberosum) cultivars to PYBV and PVA was similar. In over 5 years of investigation, PYBV has not been found in commercial seed and ware potato crops in Scotland.  相似文献   

Fatty acid composition of lipids of Australian oats     

M.X. Zhou  M. Glennie Holmes  K. Robards  S. Helliwell 《Journal of Cereal Science》1998,28(3):311-319

A method using methanolic sulphuric acid as transmethylating reagent was developed for determining the fatty acid composition of lipids of oats. The method was optimised for reaction conditions and applied to the determination of the fatty acid composition of lipids of a number of varieties of Australian oats grown in several locations. Thirteen fatty acids were detected with oleic, linoleic and palmitic acids comprising more than 95% of the total fatty acids. Total lipid content of the oats was positively related to the proportion of stearic (r=0·32) and oleic (r=0·81) acids and negatively correlated with the proportion of palmitic (r=−0·64), linoleic (r=−0·39) and linolenic (r=−0·65) acids. Significant positive correlations were found between total lipid content and absolute content of the major fatty acids (r=0·670·98), except for linolenic acid (r=0·12). Environment had significant effects on fatty acid composition, but variety was the controlling factor. The broad sense heritability estimated from individual plot ranged from 69 to 73% and that from the average of three replications and eight locations ranged from 94 to 98% for the major fatty acids. It is possible to improve fatty acid composition of oats by breeding procedures.  相似文献