Isolation of Theileria sergenti piroplasms from infected erythrocytes and development of an enzyme-linked immunosorbent assay for serodiagnosis of T sergenti infections     

S Shimizu  K Suzuki  K Nakamura  K Kadota  K Fujisaki  S Ito  T Minami 《Research in veterinary science》1988,45(2):206-212

A method of obtaining a pure suspension of Theileria sergenti piroplasms from infected bovine erythrocytes was developed and the resulting parasites used as antigen in an enzyme-linked immunosorbent assay (ELISA). Piroplasms were freed from infected erythrocytes using the nitrogen cavitation technique and then separated from unbroken erythrocytes by centrifugation at 670 gmax. The parasites in the supernatant were then sedimented by centrifugation at 2700 gmax and the purified fraction examined by electron microscopy. This examination showed that the isolated piroplasms were morphologically intact and that there were few contaminants. SDS-PAGE and spectrophotometric analysis showed that this fraction contained little erythrocyte component. The piroplasms thus obtained were sonicated and treated with Triton X-100 then used for ELISA antigen. The ELISA values had a high correlation with titres obtained using the indirect fluorescent antibody test on sera from cattle infected with T sergenti.  相似文献   

Enzyme-linked immunosorbent assay using solubilised antigen for detection of antibodies toAnaplasma marginale   总被引：1，自引：0，他引：1  

Yoshio Nakamura  Shinya Shimizu  Tetsuro Minami  Shingo Ito 《Tropical animal health and production》1988,20(4):259-266

An enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies against Anaplasma marginale. A marginale bodies were separated from parasitised erythrocytes by a modified nitrogen decompression method, sonicated, then solubilised with Triton X-100 and used as the ELISA antigen. In this ELISA system the required amount of antigen protein was 16.2 ng for each well. In the course of experimental infections, of calves, significant antibody levels were detected by ELISA and the complement fixation test (CFT) at almost the same time. Antibodies against A. marginale were detectable for longer periods using the ELISA than using the CFT. Sera from calves infected with Babesia bigemina, B. bovis, B. ovata, Theileria sergenti and Eperythrozoon wenyoni gave no reaction; however, antisera against A. centrale did react with the A. marginale ELISA antigen.  相似文献   

An epidemic of multiresistant Salmonella typhimurium with a characteristic plasmid profile among calves in Tokachi district, Japan     

M Tanabe  N Ishiguro  M Shinagawa  G Sato  N Seno  H Ohnuma  A Nakamura 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(5):1025-1034

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Determination of a novel bitter amino acid, pulcherrimine, in the gonad of the green sea urchin Hemicentrotus pulcherrimus     

Yuko Murata  Noriko U Sata  Masahito Yokoyama  Ryuji Kuwahara  Masaki Kaneniwa  Ichiro Oohara 《Fisheries Science》2001,67(2):341-345

SUMMARY: A novel bitter amino acid, pulcherrimine was analyzed by reverse-phase high-performance liquid chromatography using a linear gradient elution consisting of methanol/aqueous/acetic acid and methanol/acetic acid mixture, after being labeled with dimethylaminoazobenzenesulfonyl chloride (Dabs-Cl). Linearity of the calibration curve for pulcherrimine was high ( r = 0.994) in the tested range from 0 to 4 μg/mL. By this method, the pulcherrimine content in the ovary and testis of the green sea urchin Hemicentrotus pulcherrimus , collected in the sea off Iwaki in November 1998, was analyzed. The mean pulcherrimine content in the ovary was 1.37 mg/100 g. In contrast, no pulcherrimine was detected in all testes examined. Correlation between the bitterness and the pulcherrimine content was statistically significant. These results strongly support that pulcherrimine is a bitter principle in the green sea urchin ovaries.  相似文献   

Outbreak of pneumonia caused by Pasteurella haemolytica infection in Shiba goats in Japan     

H Hayashidani  E Honda  T Nakamura  Y Mori  T Sawada  M Ogawa 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(4):960-962

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Vascular architecture of N-methyl nitrosourea-induced rat papillary adenoma demonstrated by scanning electron microscopy of resin casts     

H Ninomiya  T Nakamura 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(5):1065-1073

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Dual infections of Eimeria tenella and Escherichia coli in chickens     

K Nakamura  T Isobe  M Narita 《Research in veterinary science》1990,49(1):125-126

The interactive effects of Eimeria tenella and Escherichia coli infection in chickens were investigated. Specific pathogen free chickens inoculated orally with E tenella and challenged four days later with E coli via the air sac showed more severe acute septicaemic lesions and subacute serositis than chickens given E coli alone. Moreover, caecal lesions induced by E tenella were more severe in chickens given both E tenella and E coli than in those given E tenella alone. In contrast, oral inoculation of E coli did not result in acute septicaemic lesions or subacute serositis and had no effect on the severity of the caecal lesions caused by E tenella.  相似文献   

Theileria sergenti: a simple method for isolation of piroplasms from erythrocytes     

K Fujisaki  T Kamio  Y Nakamura  K Shimura  Y Takahashi  S Kawazu  S Shimizu  T Minami  S Ito 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(2):457-459

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The Capillary Circulation in the Penile Skin of the Dog     

Hiroyoshi  Ninomiya  Tsunenori  Nakamura 《Anatomia, histologia, embryologia》1981,10(4):361-369

The microcirculation of the penile skin of the dog is described. This study is based on the examination of the penile skin vasculature of 14 adult dogs in which injection cast preparations of the capillary systems had been made. The capillary organization was examined with the aid of a scanning electron microscope. The organization of the capillary system and associated microregulatory vessels are presented as a model for further investigation of micro-circulatory anatomy and physiology.  相似文献   

Interferon induction by avian infectious bronchitis virus in chickens     

K Otsuki  T Nakamura  Y Kawaoka  M Tsubokura 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1982,44(3):535-537

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