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61.
Effects of β‐carotene‐enriched dry carrots on β‐carotene status and colostral immunoglobulin in β‐carotene‐deficient Japanese Black cows 下载免费PDF全文
Yoshitaka Nishijima Saya Taniguchi Shuntaro Ikeda Kenichi Yoshitani Takashi Hamano Hiroshi Tani Masahiko Fujita Kenji Murakami Keisuke Kogusa Kenji Sato Miki Sugimoto Shinichi Kume 《Animal Science Journal》2017,88(4):653-658
Data from 18 β‐carotene‐deficient Japanese Black cows were collected to clarify the effects of feeding β‐carotene‐enriched dry carrots on β‐carotene status and colostral immunoglobulin (Ig) in cows. Cows were assigned to control or carrot groups from 3 weeks before the expected calving date to parturition, and supplemental β‐carotene from dry carrots was 138 mg/day in the carrot group. Plasma β‐carotene concentrations in the control and carrot groups at parturition were 95 and 120 μg/dL, and feeding dry carrots slightly improved plasma β‐carotene at parturition. Feeding dry carrots increased colostral IgA concentrations in cows and tended to increase colostral IgG1, but colostral IgM, IgG2, β‐carotene and vitamin A were not affected by the treatment. Feeding dry carrots had no effects on plasma IgG1, IgA and IgM concentrations in cows, but plasma IgG1 concentrations decreased rapidly from 3 weeks before the expected calving date to parturition. These results indicate that feeding β‐carotene‐enriched dry carrots is effective to enhance colostral IgA and IgG1 concentrations in β‐carotene‐deficient cows. 相似文献
62.
Kentaro Ikeda 《Journal of General Plant Pathology》2010,76(4):241-246
Pepper (Piper nigrum L.) root rot caused by Fusarium solani f. sp. piperis (FSP; teleomorph: Nectria haematococca f. sp. piperis) includes two symptom types called root rot (RR) type and stem rot (SR) type. In this study, the temporal and spatial associations
between perithecial formation of FSP and development of SR were investigated in naturally infested fields to verify the hypothesis
that ascospores from the perithecia are the major inoculum source of the SR type on vines in the field. In surveys of all
vines in two neighboring pepper fields every month from December 2005 to November 2006, I mapped the locations of all vines
with perithecia and all vines the SR type. The frequency of vines with perithecia increased during April and May, the late
rainy season. In June, the early dry season, the number of vines with SR type greatly increased. The vertical range of perithecial
formation on the vines extended to 200 cm in height, but was restricted to 30 cm in the dry season in both fields. The join-count
statistics showed a significant spatial association between vines with perithecia and vines with SR type in one field (P = 0.042), while no significant spatial association was recognized in another field. The results suggested that ascospores
from perithecia of FSP on pepper vines are likely to be one of the main inoculum sources of the SR type of the disease on
adjacent vines, but they may not be the exclusive source. 相似文献
63.
A 15‐year‐old, spayed, female poodle dog was presented for evaluation of a mass of tissue prolapsed from the vulva. The dog had been hysterectomized when it was 5 years old. A vaginal mass had been removed approximately 10 months before presentation. Haematological and serum biochemistry analyses demonstrated mild leucocytosis and glycaemia. A vaginal smear was predominantly made up of parabasal cells and intermediate cells with no neoplastic cells. Thoracal and abdominal radiographic findings were unremarkable. The ovaries could not be identified using abdominal ultrasonography. A midline exploratory laparotomy identified both ovaries that were surgically excised. The vaginal mass was also removed following an episiotomy procedure. Histopathological examination of the mass demonstrated that it was a neurofibroma. Both ovaries had cystic changes. Four months after the surgery, the owner reported that the dog was clinically normal. To the authors’ knowledge, this is the first reported case of a vaginal neurofibroma after an incomplete ovariohysterectomy in the dog. 相似文献
64.
65.
Schoenoplectus juncoides, a noxious weed for paddy rice, is known to become resistant to sulfonylurea (SU) herbicides by a target-site mutation in either of the two acetolactate synthase (ALS) genes (ALS1 and ALS2). SU-resistant S. juncoides plants having an Asp376Glu mutation in ALS2 were found from a paddy rice field in Japan, but their resistance profile has not been quantitatively investigated. In this study, dose–response of the SU-resistant accession was compared with that of a SU-susceptible accession at in vivo whole-plant level as well as at in vitro enzymatic level. 相似文献
66.
Tohei A Kojima S Ikeda M Hokao R Shinoda M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(7):901-906
It is known that pica, the consumption of non-nutritive substances such as kaolin, can be induced by administration of toxins or emetic agents in rats. In the present study, we examined the effects of intraperitoneal (i.p.) administration of cyclophosphamide on pica behavior and on the concentration of 5-hydroxyindoleacetic acids (5HIAA) in cerebrospinal fluid (CSF) in the following five strains of adult male rats: Sprague Dawley (SD), Wistar, Fischer 344 (F344), Wistar-Imamichi (WI) and Long Evans (LE). Cyclophosphamide (25 mg or 50 mg/kg) was injected (i.p.) into the rats and kaolin and food intake were measured at 24 hr after injection. The animals were anesthetized with urethane (1 g/kg) at 3 hr after injection of cyclophosphamide, and CSF was collected from the cisterna magna. WI and LE rats clearly showed pica behavior as compared with the other strains. In LE rats, the concentration of 5HIAA in CSF also increased in a dose-dependent manner of cyclophosphamide. The pretreatment with ondansetron (5-HT(3) antagonist) restored both changes (kaolin consumption and 5HIAA levels) induced by cyclophosphamide. These results suggest that the LE rat is sensitive to cyclophosphamide, that pica induced by cyclophosphamide mimics many aspects of emesis including the serotonergic response in the central nervous system and that use of the pica model would be a practical method for evaluating the effects of antiemetic drugs in addition to the mechanism of emesis. 相似文献
67.
Marcondes M Ikeda FA Vieira RF Day MJ Lima VM Rossi CN Perri SH Biondo AW 《Veterinary parasitology》2011,181(2-4):153-159
Canine Visceral Leishmaniasis (CVL) is a widespread zoonotic disease with mandatory euthanasia of infected dogs determined by the Brazilian Ministry of Health. Development of vaccines against CVL may provide a prophylactic barrier, but transitory peak of antibody response detected by standard diagnostic techniques in vaccinated dogs may be interpreted as natural infection. Accordingly, the aim of the present study was to sequentially evaluate total and IgG subclasses response between naturally Leishmania-infected and dogs vaccinated with Leishmune(?). A total of 172 mongrel dogs were divided in four groups: Group 1 (G1) with 45 clinically healthy dogs, Group 2 (G2) and Group 3 (G3) with 45 dogs naturally infected by Leishmania sp. each, symptomatic and asymptomatic respectively, and G4 (G4) with 37 healthy dogs submitted to a complete protocol of a commercially available vaccine against CVL, monitored and evaluated in 5 different chronological moments (M0-M4) up to 180 days after M0. Total IgG, IgG1 and IgG2 were unable to differentiate between infected (G2 and G3) and vaccinated (G4) dogs, demonstrating that polyclonal commercial antibodies do not distinguish these groups apart. Total and IgG subclasses antibodies were not detected until 21 days of the second vaccination dose; however, seroconversion was observed on 21 days and sustained positivity up to 6 months after the vaccination start. A peak of antibodies response was observed on 90 days (M3), when results for total IgG, IgG1, IgG2, IgG3 and IgG4 where highly significant when compared to M0 (P<0.0001). Neither total IgG nor IgG1 effectively differentiated between infected (G2 and G3) and vaccinated (G4) dogs. In conclusion, despite dogs may test serologically negative immediately after vaccination against CVL with Leishmune(?), subsequent seroconversion, antibody peak and positivity up to six months may lead vaccinated dogs to be mistakenly identified as naturally infected dogs during this period. 相似文献
68.
Fujiwara Y Hayashida A Tsurushima K Nagai R Yoshitomi M Daiguji N Sakashita N Takeya M Tsukamoto S Ikeda T 《Journal of agricultural and food chemistry》2011,59(9):4544-4552
Because foam cell formation in macrophages is believed to play an essential role in the progression of early atherosclerotic lesions in vivo, prevention of foam cell formation is considered to be one of the major targets for the treatment of atherosclerosis. The present study examined the inhibitory effect of 50 crude plant extracts on foam cell formation. Among those crude extracts, Zizyphi Fructus (ZF) and Zizyphi Semen (ZS) extracts significantly inhibited the foam cell formation induced by acetylated LDL. Furthermore, triterpenoids such as oleanonic acid, pomolic acid, and pomonic acid were the major active compounds, and triterpenoids containing a carboxylic acid at C-28 play an important role in the inhibitory effect on foam cell formation in human macrophages. These data suggest that triterpenoids in Zizyphus jujuba , the plant source of ZF and ZS, may therefore be useful for the prevention of atherosclerosis. 相似文献
69.
Jun-ichi Ikeda 《Soil Science and Plant Nutrition》2013,59(4):703-710
To characterize the regulation site and manner of the abundant nodulation in the soybean (Glycine max (L.) Merr.) cv. Kitamusume, three grafting eperiments were carried out as follows: reciprocal wedge grafting and inter-cultivar approach grafting between Kitamusume and a normal nodulating cultivar, Toyosuzu, as well as wedge grafting of scions of the supernodulating mutant En6500 onto either Kitamusume or Toyosuzu rootstock. In the reciprocal wedge grafting, the number of nodules per shoot dry weight and average weight per nodule in the grafted plants were consistent with those exhibited by the genotype of their rootstocks. Approach grafting did not affect the number of nodules per shoot dry weight on either side of the inter-cultivar approachgrafted plant. Although grafting of the mutant scion resulted in the loss of the autoregulatory response from the roots of both cultivars, difference in the number of nodules per g shoot dry weight still remained between the two cultivars. These results suggested that the abundant nodulation in Kitamusume is controlled by the root in a non-systemic manner and is independent of the autoregulation mechanism. 相似文献
70.
Ground-dwelling beetle community in beech forests of Sado Island,Japan: comparison with the mainland
Island ecosystems differ from mainland ecosystems in their long-term isolation. On Sado Island, Japan, cattle grazing in the
past gradually changed forests into grasslands, thereby altering the diversity of indigenous insects. We compared ground-dwelling
beetle diversity on Sado Island to that on the nearby mainland. Contrary to previous reports, the island isolation effect
resulted in lower γ and β diversity than on the mainland, because of the low species richness of beetles with low dispersal
abilities. Few flightless beetles could invade Sado Island, even when the beech forests on the island were relatively adjacent
to those on the mainland. Such a history of ecosystem formation could be the general pattern for continental-island mountain
forests that were never connected to those of the mainland. We did not observe low α diversity of ground-dwelling beetles,
suggesting that the cattle grazing history of the island has not changed environmental conditions for ground-dwelling beetles
enough to reduce their diversity. In addition, cattle grazing increases the abundance of merdivorous insects by increasing
the abundance of their food resource.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献