Expression of porcine FSHbeta subunit promoter-driven herpes simplex virus thymidine kinase gene in transgenic rats     

Cai LY  Kato T  Ito K  Nakayama M  Susa T  Aikawa S  Maeda K  Tsukamura H  Ohta A  Izumi S  Kato Y 《The Journal of reproduction and development》2007,53(2):201-209

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Molecular cloning and expression analysis of the BPI/LBP cDNA and its gene from ayu <Emphasis Type="Italic">Plecoglossus altivelis altivelis</Emphasis>     

Kyuma Suzuki  Shotaro Izumi  Hideki Tanaka  Takayuki Katagiri 《Fisheries Science》2009,75(3):673-681

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Isolation and characterization of an additional crustacean hyperglycemic hormone from the greasyback shrimp <Emphasis Type="Italic">Metapenaeus ensis</Emphasis>     

Tsuyoshi Ohira  Katsuyoshi Suitoh  Fumihiro Yamane  Chiaki Nagai  Michio Suzuki  Naoaki Tsutsui  Hiromichi Nagasawa  Susumu Izumi 《Fisheries Science》2010,76(4):605-611

Crustacean hyperglycemic hormone (CHH) is released from the X-organ/sinus gland complex located in the eyestalks. In this study, the most abundant CHH in the sinus gland of the greasyback shrimp Metapenaeus ensis was purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. Although two CHH molecules (Mee-CHH-A and Mee-CHH-B) have already been identified from M. ensis by cDNA cloning, this study revealed the presence of an additional CHH peptide based on differences in the N-terminal amino acid sequences of the CHH-A and CHH-B. Therefore, this novel CHH was designated as Mee-CHH-C. A cDNA encoding the Mee-CHH-C precursor was cloned by RT-PCR coupled with 5′- and 3′-RACE, and it was found that the mature Mee-CHH-C consisted of 72 amino acid residues containing 6 conserved cysteine residues and possessed an amidated C terminus. Mee-CHH-C had 62 and 68% identities with Mee-CHH-A and Mee-CHH-B, respectively, and was highly homologous to CHHs characterized from other penaeid shrimp species. The hyperglycemic activity of Mee-CHH-C was examined by an in vivo bioassay using the kuruma prawn Marsupenaeus japonicus. Injection of Mee-CHH-C increased hemolymph glucose levels significantly and dose-dependently. These results indicate that Mee-CHH-C is possibly one of the major molecules in M. ensis that regulate glucose levels in the hemolymph.  相似文献   

Relationship between abundance and localization of maternal messenger RNA and egg quality in artificially matured Japanese eel Anguilla japonica     

Horiuchi  Moemi  Izumi  Hikari  Lokman  P. Mark  Ijiri  Shigeho  Adachi  Shinji 《Fisheries Science》2020,86(1):43-56

Fisheries Science - Artificial maturation and ovulation of Japanese eel do not always result in the production of good-quality eggs. The molecular basis for compromised egg quality in artificially...  相似文献   

Molecular typing by pulsed-field gel electrophoresis of Flavobacterium psychrophilum isolates derived from Japanese fish     

Arai H  Morita Y  Izumi S  Katagiri T  Kimura H 《Journal of fish diseases》2007,30(6):345-355

Sixty-four isolates of Flavobacterium psychrophilum from ayu, Plecoglossus altivelis altivelis (Temminck & Schlegel), and other fish (n=16) in Japan and the type strain (NCIMB 1947(T)) were typed using pulsed-field gel electrophoresis (PFGE) with endonuclease BlnI and XhoI. These isolates were classified into 20 clusters and 42 genotypes by PFGE analysis. The most predominant cluster of isolates from ayu was cluster XII (n=20), followed by clusters XVII, XVI, XX, XI, IX, X, XIII and XV; the remaining 17 isolates from other fish were divided into clusters I, II, III, IV, V, VI, VII, VIII, XIV, XVIII and XIX. The PFGE genotype of isolates from ayu clearly differed from those of other fish. The isolates from ayu in Gunma Prefecture belonged to clusters XII, XVI, XVII and XX, and the strains of three of these clusters (XII, XVII and XX) were isolated from ayu in 15 of 19 prefectures. PFGE typing enabled more accurate classification of isolates into clusters than previously achieved by analysing the restriction fragment length polymorphism of PCR products. These results suggest that F. psychrophilum isolated from ayu and other fish are genetically different and strains with several PFGE types have spread within Japan.  相似文献   

Ultrafast viscous permeation of organic solvents through diamond-like carbon nanosheets     

Karan S  Samitsu S  Peng X  Kurashima K  Ichinose I 《Science (New York, N.Y.)》2012,335(6067):444-447

Chemical, petrochemical, energy, and environment-related industries strongly require high-performance nanofiltration membranes applicable to organic solvents. To achieve high solvent permeability, filtration membranes must be as thin as possible, while retaining mechanical strength and solvent resistance. Here, we report on the preparation of ultrathin free-standing amorphous carbon membranes with Young's moduli of 90 to 170 gigapascals. The membranes can separate organic dyes at a rate three orders of magnitude greater than that of commercially available membranes. Permeation experiments revealed that the hard carbon layer has hydrophobic pores of ~1 nanometer, which allow the ultrafast viscous permeation of organic solvents through the membrane.  相似文献   

Iron derivatives from casein hydrolysates as a potential source in the treatment of iron deficiency   总被引：1，自引：0，他引：1  

Chaud MV  Izumi C  Nahaal Z  Shuhama T  Bianchi Mde L  de Freitas O 《Journal of agricultural and food chemistry》2002,50(4):871-877

The properties of an Fe(3+)-peptide complex containing 5.6% Fe, obtained by the reaction of ferric chloride with an enzymatic hydrolysate of casein, are described. The major site of iron binding corresponds primarily to the carboxylate groups and to a lesser extent to the peptide bonds. The Fe(3+)-peptide complex is insoluble at acid pH and completely soluble at neutral to alkaline pH. When soluble, the Fe(3+) is tightly bound to the complex peptide mixture but can be displaced and complexed by a low molecular weight ligand such as cysteine. Its efficacy in relation to iron sulfate was compared in rats. Both iron sources were administrated in Milli-Q water by gastric gavage to male Wistar rats (180-200 g) after an 18 h fast with water ad libitum. Fe(3+) from the Fe(3+)-peptide complex was transferred to the blood in a dose-dependent manner (1-8 mg of Fe/kg), and the serum iron levels were significantly higher (p < 0.001) than in a similar group of rats treated with iron sulfate. In the comparative kinetics experiments, the rats received 4 mg of Fe/kg. Both iron sources presented maximum absorption, as indicated by the elevation of serum iron levels, 30 min after administration, and the AUC(0)(-->2h) of the Fe(3+)-peptide complex was significantly higher (p < 0.05) than that observed with iron sulfate. The simultaneous administration of free peptides (0-192 mg) with the Fe(3+)-peptide complex or iron sulfate did not modify the extent of absorption of iron from both sources, suggesting that the absorption is due to the complex formed and probably not to exchange reactions in the gastrointestinal tract. In the hemoglobin repletion experiments carried out on newly weaned rats with anemia induced by a low-iron diet, supplementation of the diet with the the Fe(3+)-peptide complex was as efficient as supplementation with iron sulfate in the conversion from diet to hemoglobin iron. These results, taken together, suggest that the Fe(3+)-peptide complex is a potential compound for use as an iron source in biological situations.  相似文献   

Mating rhythms of Drosophila: rescue of tim⁰¹ mutants by D. ananassae timeless     

Izumi Nishinokubi  Masami Shimoda  Norio Ishida 《Journal of circadian rhythms》2006,4(1):4

Background  

It is reported that the circadian rhythms of female mating activity differ among Drosophila species and are controlled by an endogenous circadian clock. Here, we found that the mating rhythm of D. ananassae differed from that of D. melanogaster. Moreover, to evaluate the effect of clock gene products on mating activities, we examined the mating activity of D. melanogaster timeless (tim ⁰¹) transgenic fly harboring heat-shock promotor driven-D. ananassae timeless (tim) gene (hs-AT tim ⁰¹).  相似文献   

The Semaphorin 4D receptor Plexin-B1 is a GTPase activating protein for R-Ras     

Oinuma I  Ishikawa Y  Katoh H  Negishi M 《Science (New York, N.Y.)》2004,305(5685):862-865

Plexins are cell surface receptors for semaphorin molecules, and their interaction governs cell adhesion and migration in a variety of tissues. We report that the Semaphorin 4D (Sema4D) receptor Plexin-B1 directly stimulates the intrinsic guanosine triphosphatase (GTPase) activity of R-Ras, a member of the Ras superfamily of small GTP-binding proteins that has been implicated in promoting cell adhesion and neurite outgrowth. This activity required the interaction of Plexin-B1 with Rnd1, a small GTP-binding protein of the Rho family. Down-regulation of R-Ras activity by the Plexin-B1-Rnd1 complex was essential for the Sema4D-induced growth cone collapse in hippocampal neurons. Thus, Plexin-B1 mediates Sema4D-induced repulsive axon guidance signaling by acting as a GTPase activating protein for R-Ras.  相似文献   

Formation and Deposition of Ozone in a Red Pine Forest     

Utiyama  Masahiro  Fukuyama  Tsutomu  Yamada Maruo  Yasuko  Ichino  Toshihiro  Izumi  Katsuyuki  Hara  Hiroshi  Takano  Kenichi  Suzuki  Haruko  Aoki  Masatoshi 《Water, air, and soil pollution》2004,151(1-4):53-70

Concentrations of ozone and nitrogen oxides, together with air temperature and solar radiation intensity, were measured at several heights on a tower standing through the canopy of a red pine forest in summer and in autumn. In the summer observation, the diurnal variation patterns of ozone concentration both above and below the canopy were all similar and parallel to the solar radiation intensity. Using the data collected immediately above the canopy, deviation from the Leighton relationship and variations of concentration sums [O₃] + [NO] and [NO₂] + [NO] were examined, and as a result, it was supposedthat ozone was photochemically formed there in the daytime, probably because hydrocarbons emitted from pine trees broke the photostationary state among ozone and nitrogen oxides. The vertical temperature profile exhibited an inversion at the leaf-layer, which must have hindered vertical mixing of the air and made the trunk space more or less isolated from the upper atmosphere. These observations led to an idea that the similarity of the ozone variation pattern at every height was caused by the photochemical formation that proceeded simultaneously above and below the canopy rather than by vertical transport. Such situations of ozone formation were supported by observation of two maximums in the ozone vertical profile, one immediately above the canopy and another in the trunk space. Another feature of the ozone profile was a deep minimum in the leaf layer, which indicated ozone deposition onto leaf surfaces. This study thus revealed concurrence of ozone formation and deposition, and left two potentially important implications worthy of further investigation: (1) a forest is not always a sink but can be a source of ozone in sunlit conditions, and (2) deposition of ozone to trees can take place not only from outside but also from inside of a forest. In the autumn observation, however, the ozone formation was barely recognizable above the canopy and no longer found in the trunk space; in addition, the ozone concentration minimum in the leaf layer disappeared, suggesting that the deposition or removal was dependent on temperature.  相似文献