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101.
Jackson HH 《Science (New York, N.Y.)》1914,39(1006):534-535
102.
Posttranslational modifications of the histone octamer play important roles in regulating responses to DNA damage. Here, we reveal that Saccharomyces cerevisiae Rtt109p promotes genome stability and resistance to DNA-damaging agents, and that it does this by functionally cooperating with the histone chaperone Asf1p to maintain normal chromatin structure. Furthermore, we show that, as for Asf1p, Rtt109p is required for histone H3 acetylation on lysine 56 (K56) in vivo. Moreover, we show that Rtt109p directly catalyzes this modification in vitro in a manner that is stimulated by Asf1p. These data establish Rtt109p as a member of a new class of histone acetyltransferases and show that its actions are critical for cell survival in the presence of DNA damage during S phase. 相似文献
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Effects of basal debris on glacier flow 总被引：1，自引：0，他引：1
Iverson NR Cohen D Hooyer TS Fischer UH Jackson M Moore PL Lappegard G Kohler J 《Science (New York, N.Y.)》2003,301(5629):81-84
Glacier movement is resisted partially by debris, either within glaciers or under glaciers in water-saturated layers. In experiments beneath a thick, sliding glacier, ice containing 2 to 11% debris exerted shear traction of 60 to 200 kilopascals on a smooth rock bed, comparable to the total shear traction beneath glaciers and contrary to the usual assumption that debris-bed friction is negligible. Imposed pore-water pressure that was 60 to 100% of the normal stress in a subglacial debris layer reduced shear traction on the debris sufficiently to halt its deformation and cause slip of ice over the debris. Slip resistance was thus less than debris shearing resistance. 相似文献
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The gradual closure of the Panamanian seaway and the resulting environmental change stimulated an increase in Caribbean molluscan diversity rather than the mass extinction hypothesized previously on the basis of inadequate data. Upheaval of molluscan faunas did occur suddenly throughout tropical America at the end of the Pliocene as a result of more subtle, unknown causes. There is no necessary correlation between the magnitude of regional shifts in abiotic conditions and the subsequent biological response. 相似文献
110.
Fluorogenic substrate detection of viable intracellular and extracellular pathogenic protozoa 总被引：24，自引：0，他引：24
Viable Leishmania promastigotes and amastigotes were detected by epifluorescence microscopy with fluorescein diacetate being used to mark living parasites and the nucleic acid-binding compound ethidium bromide to stain dead cells. This procedure is superior to other assays because it is faster and detects viable intracellular as well as extracellular Leishmania. Furthermore, destruction of intracellular pathogens by macrophages is more accurately determined with fluorescein diacetate than with other stains. The procedure may have applications in programs to develop drugs and vaccines against protozoa responsible for human and animal disease. 相似文献