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101.
The study was conducted under the “Uttar Pradesh Sodic Lands Reclamation Project” to examine changes that occurred in the reclaimed sodic land in two districts of Uttar Pradesh, India. The study focuses on long‐term seasonal changes in the floral diversity and soil characteristics of the reclaimed sodic land over a period of 10 y. The changes in the floristic composition, plant density, and soil characteristics (microbial biomass carbon [MBC], pH, exchangeable‐sodium percentage (ESP), and electrical conductivity) were compared among the different study plots after different years of sodic‐land reclamation. The study plots comprised reclaimed land with rice–wheat cultivation; semireclaimed land under rice cultivation only and nonreclaimed barren sodic land. There was a significant variation in the floristic composition of the three study plots. Dominance in the floristic composition was shifted from monocotyledonous weeds in the nonreclaimed sodic land to dicotyledonous weeds in the reclaimed land after 10 y of reclamation. Among the soil characteristics, the most remarkable changes were observed in soil MBC and ESP during the course of sodic‐land reclamation. Soil MBC increased up to 480% and ESP values decreased up to 79% in the reclaimed plots with reference to the nonreclaimed plots. The soil amelioration was more pronounced in the upper layer (0–30 cm) as compared to the lower layer (below 30 cm depth). A positive significant correlation was revealed between soil MBC and floristic composition of the reclaimed plots. These changes in floristic composition and soil characteristics could be used as good indicators of the eco‐restoration of the sodic lands. The present study provides useful insights in understanding the temporal progress of eco‐restoration in the reclaimed sodic lands.  相似文献   
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Effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on in vitro survival, growth, steroidogenesis, and apoptosis of buffalo preantral follicles (PFs) was investigated. PFs (200~250 µm) were isolated by micro-dissection and cultured in 0 (control), 10-3, 10-5, 10-7, and 10-9 M SNP. To examine the reversible effect of SNP, PFs were cultured with 10-5 M SNP + 1 mM Nω-nitro-L-arginine methyl ester (L-NAME) or 1.0 µg hemoglobin (Hb). The results showed that greater concentrations of SNP (10-3, 10-5, 10-7 M) inhibited (p < 0.05) FSH-induced survival, growth, antrum formation, estradiol production, and oocyte apoptosis in a dose-dependent manner. However, a lower dose of SNP (10-9 M) significantly stimulated (p < 0.05) the survival, growth, antrum formation, follicular oocyte maturation, and stimulated progesterone secretion compared to the control. A combination of SNP + L-NAME promoted the inhibitor effect of SNP while a SNP + Hb combination reversed this effect. Nitrate and nitrite concentrations in the culture medium increased (p < 0.05) in a dose-dependent manner according to SNP concentration in the culture medium. At higher concentrations, SNP had a cytotoxic effect leading to follicular oocyte apoptosis whereas lower concentrations have stimulatory effects. In conclusion, NO exerts a dual effect on its development of buffalo PFs depending on the concentration in the culture medium.  相似文献   
104.
Although experiments have previously been carried out to determine the optimum sowing time of sesame, very few attempts have been made to determine the effects of macro and micro climatic variation on yield. Thus a field experiment was conducted at the University Research Farm, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, India on an Entisol (alluvial, sandy loam) in the summer seasons of 1996 and 1997 to assess the effects of macro and micro climatic variations on yield of sesame for various dates of sowing. The number of seeds per capsule of sesame was highest in the crop sown on 19 February, although up to the 18 April sowing there was no significant variation. There was no appreciable variation in thousand-seed weight with date of sowing. Crops sown on 19 February and 1 March produced statistically similar yields. The average reduction in yield of sesame was 78.5 and 213 %, respectively, for crops sown on 10 February and 28 April, compared with the crop sown on 19 February. The cultivar Rama produced higher yields than B-67 and Kanke-1. Ambient temperature above 30 °C up to 100 % flowering had a direct positive effect on sesame yield. Crop exposure to 60–70, 70–80 and  80 % relative humidity up to 100 % flowering had a direct negative effect. Temperature profile at 50 days after emergence (DAE), relative humidity at 60 DAE, and photosynthetically active radiation (PAR) at 30 and 40 DAE within the crop canopy had direct positive effects on yield. Regression models suggested that temperature profile and PAR within the crop canopy produced 72 and 35 % variation in yield, respectively.  相似文献   
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Genetic Resources and Crop Evolution - The present paper deals with the report on the potential of Vigna angularis var. nipponensis (Ohwi) Ohwi and H. Ohashi assembled from Northeastern hill region...  相似文献   
108.
The ITS1-5.8S-ITS2 region of the 18S-28S nrDNA was sequenced in 49 Vicia species and subspecies selected from nine sections of the two subgenera to resolve taxonomic contradictions. Phylogenies derived from the internal transcribed spacer (ITS) sequences strongly support the view that both Vicia faba and Vicia bithynica are distant from the Narbonensis species complex (NSC) in section Faba. V. faba is more closely aligned with sections Peregrinae and Hypechusa, whereas V. bithynica is more closely aligned with section Vicia. Six synapomorphic substitutions unique to the NSC support a separate section for the Narbonensis complex. Vicia johannis subsp. johannis was the most diverged species within the NSC with high genetic distance between V. johannis subsp. johannis and remaining species and subspecies of the NSC. These results suggest that Vicia johannis subsp. johannis should be given species rank. The neighbor-joining and parsimony based trees supported the sections Vicia (after the exclusion of Vicia lathyroides) and Cracca (after the exclusion of Vicia vicioides and Vicia hirsuta). The section Hypechusa is polyphyletic and paraphyletic based on the present ITS sequence cladistic analysis. The x = 7 as the ancestral number for the genus is supported by the present study.  相似文献   
109.
Corynebacterium pseudotuberculosis, a Gram-positive bacterium is the causative agent of caseous lymphadenitis (CLA), a chronic disease of sheep, goats and other warm blooded animals. In the present study, a total of 1,080 sheep reared under semi-intensive system on organized farms situated in the semi arid tropical region of Rajasthan, India, was clinically examined. Pus samples from superficial lymph nodes of 25 (2.31 %) adult sheep showing clinical lesions similar to CLA were collected for laboratory analyses. On the basis of morphological, cultural and biochemical characteristics 12 (48 %) bacterial isolates from pus identified it as C. pseudotuberculosis. A polymerase chain reaction (PCR) assay targeting Putative oligopeptide/dipeptide ABC transporter, nicotinamide adenine dinucleotide phosphate (NADP) oxidoreductase coenzyme F420-dependent and proline iminopeptidase (PIP) genes of C. pseudotuberculosis was developed that showed 14 pus samples as positive. All C. pseudotuberculosis isolates were also found positive for these genes in the PCR. The specificity of the PCR products was confirmed by sequencing of the amplified products that showed 98–100 % homology with published sequences available in the NCBI database. The present study shows the incidence of CLA as 2.31 %, 1.1 % and 1.29 % based on clinical, bacterial culture and direct pus PCR assay, respectively. The PCR assay was rapid, specific and as significant as bacterial culture in detecting bacteria directly in the clinical pus samples. The PCR assay developed in the study can be applied for the diagnosis and control of CLA. Furthermore, the assay can also be applied to detect C. pseudotuberculosis in various clinical samples.  相似文献   
110.
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