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Taste disturbances following consumption of pine nuts, referred to as "pine mouth", have been reported by consumers in the United States and Europe. Nuts of Pinus armandii have been associated with pine mouth, and a diagnostic index (DI) measuring the content of Δ5-unsaturated fatty acids relative to that of their fatty acid precursors has been proposed for identifying nuts from this species. A 100 m SLB-IL 111 GC column was used to improve fatty acid separations, and 45 pine nut samples were analyzed, including pine mouth-associated samples. This study examined the use of a DI for the identification of mixtures of pine nut species and showed the limitation of morphological characteristics for species identification. DI values for many commercial samples did not match those of known reference species, indicating that the majority of pine nuts collected in the U.S. market, including those associated with pine mouth, are mixtures of nuts from different Pinus species.  相似文献   
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Butternut (Juglans cinerea), a tree native to North America and Japanese walnut (J. ailantifolia), native to Japan, form the basis for a small but growing specialty nut crop industry in the eastern United States and Canada. Over the last century, Canadian and American nut tree growers, academic plant breeders and nursery owners have clonally propagated cultivars and freely exchanged scion wood, creating the possibility of misidentification. To detect homonymy, synonymy and hybrid misassignments, we used 12 highly polymorphic microsatellites to genotype most of the butternut and Japanese walnut cultivars grown in the United States and Canada. Bayesian clustering and identity analyses revealed high levels of homonymies (genetically different cultivars with the same name) and synonymies (genetically identical cultivars with different names). Over 41% of the butternut cultivars and 45% of the Japanese walnut cultivars tested had homonymies, and a majority of the cultivars in both species also had synonymies. Further, 43 entries (~15%) identified by the contributors as either J. cinerea or J. ailantifolia were interspecific hybrids. Given our results we recommend that any comparative studies on the cultivars of either species or their hybrids include reference genetic fingerprints as a part of the study.  相似文献   
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ABSTRACT: For the past decade, the Food and Agriculture Organization of the United Nations has been working toward eradicating rinderpest through vaccination and intense surveillance by 2012. Because of the potential severity of a rinderpest epidemic, it is prudent to prepare for an unexpected outbreak in animal populations. There is no immunity to the disease among the livestock or wildlife in the United States (US). If rinderpest were to emerge in the US, the loss in livestock could be devastating. We predict the potential spread of rinderpest using a two-stage model for the spread of a multi-host infectious disease among agricultural animals in the US. The model incorporates large-scale interactions among US counties and the small-scale dynamics of disease spread within a county. The model epidemic was seeded in 16 locations and there was a strong dependence of the overall epidemic size on the starting location. The epidemics were classified according to overall size into small epidemics of 100 to 300 animals (failed epidemics), epidemics infecting 3 000 to 30 000 animals (medium epidemics), and the large epidemics infecting around one million beef cattle. The size of the rinderpest epidemics were directly related to the origin of the disease and whether or not the disease moved into certain key counties in high-livestock-density areas of the US. The epidemic size also depended upon response time and effectiveness of movement controls.  相似文献   
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A 15-day-old American Quarter horse colt was presented for depression and pyrexia. Peritonitis was diagnosed following peritoneal fluid analysis. Exploratory laparotomy revealed an area of focal necrosis over the dorsal wall of the urinary bladder leading to peritonitis and uroperitoneum. The affected area of the urinary bladder was resected and the peritonitis resolved with medical treatment.  相似文献   
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Immunity and mastitis. Some new ideas for an old disease.   总被引:14,自引:0,他引:14  
The biggest challenge for host immune defense against mastitis-causing bacteria in dairy cows is to quickly recruit large enough numbers of opsonizing molecules and mature neutrophils into milk such that intramammary pathogens are cleared before they multiply significantly and the inflammatory response gets out of control. Currently, this challenge is best facilitated when established mastitis control procedures are practiced on the farm, including proper hygiene, milking procedures, and regular administration of approved mastitis vaccines. However, mastitis is still a significant problem. New animal functional genomics research is beginning to allow scientists to solve the puzzle of mastitis susceptibility. Results of this type of research offer the hope of giant leaps toward a clear identification of molecular genetic variation and potential gene targets for therapies and immune manipulations that could significantly reduce the risk of clinical mastitis in traditionally susceptible cows.  相似文献   
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