OsHemA gene,encoding glutamyl-tRNA reductase(GluTR) is essential for chlorophyll biosynthesis in rice(Oryza sativa)          下载免费PDF全文

ZENG Zhao-qiong  LIN Tian-zi  ZHAO Jie-yu  ZHENG Tian-hui  XU Le-feng  WANG Yi-hua  LIU Ling-long  JIANG Ling  CHEN Sai-hua  WAN Jian-min 《农业科学学报》2020,19(3):612-623

Chlorophyll(Chl) biosynthesis is essential for photosynthesis and plant growth. Glutamyl-tRNA reductase(GluTR) catalyzes glutamyl-tRNA into glutamate-1-semialdehyde(GSA) and initiates the chlorophyll biosynthesis. Even though the main role of GluTR has been established, the effects caused by natural variations in its corresponding gene remain largely unknown. Here, we characterized a spontaneous mutant in paddy field with Chl biosynthesis deficiency, designated as cbd1. With intact thylakoid lamellar structure, the cbd1 plant showed light green leaves and reduced Chl and carotenoids(Cars) content significantly compared to the wild type. By map-based gene cloning, the mutation was restricted within a 57-kb region on chromosome 10, in which an mPingA miniature inverted-repeat transposable element(MITE) inserted in the promoter region of OsHemA gene. Both leaf color and the pigment contents in cbd1 were recovered in a complementation test, confirming OsHemA was responsible for the mutant phenotype. OsHemA was uniquely predicted to encode GluTR and its expression level was dramatically repressed in cbd1. Transient transformation in protoplasts demonstrated that GluTR localized in chloroplasts and a signal peptide exists in its N-terminus. A majority of Chl biosynthesis genes, except for POR and CHLG, were down-regulated synchronously by the repression of OsHemA, suggesting that an attenuation occurred in the Chl biosynthesis pathway. Interestingly, we found major agronomic traits involved in rice yield were statistically unaffected, except for the number of full grains per panicle was increased in cbd1. Collectively, OsHemA plays an essential role in Chl biosynthesis in rice and its weak allele can adjust leaf color and Chls content without compromise to rice yield.  相似文献   

Effects of Dietary Supplementation of Barodon on Growth Performance,Innate Immunity and Disease Resistance of Juvenile Olive Flounder,Paralichthys olivaceus,Against Streptococcus iniae     

Chang‐Hoon Shin  Hien Thi Dieu Bui  Samad Rahimnejad  Ji‐Hoon Cha  Byung‐Woo Yoo  Bo‐Kyeun Lee  Hyung‐Jin Ahn  Soo‐Il Choi  Yun‐Jeong Choi  Yong‐Ho Park  Jeong‐Dae Kim  Kyeong‐Jun Lee 《Journal of the World Aquaculture Society》2014,45(3):258-268

This study was conducted to evaluate the effects of dietary supplementation of Barodon, an anionic alkali mineral complex, on growth, feed utilization, humoral innate immunity and disease resistance of olive flounder. A basal experimental diet was used as a control and supplemented with 0.1, 0.2, 0.3, 0.4, or 0.5% Barodon. Triplicate groups of fish (26.4 ± 0.2 g) were fed one of the diets to apparent satiation twice daily for 10 wk. The growth performance was enhanced (P < 0.05) linearly and quadratically in fish fed diets containing Barodon compared with that in fish fed the control. Feed utilization was significantly improved by Barodon supplementation. Serum lysozyme and antiprotease activities were increased quadratically in Barodon fed groups. Also, significantly higher superoxide dismutase activity was found in Barodon‐fed fish. Dietary supplementation of 0.1–0.3% Barodon resulted in significant enhancement of fish disease resistance against Streptococcus iniae. The findings in this study indicate that dietary supplementation of Barodon can enhance growth, feed utilization, innate immunity, and disease resistance of olive flounder and that the optimum level seems to be 0.1% in diets.  相似文献   

屠宰生猪肺脏眼观病变的统计与分析     

林凌 《福建畜牧兽医》2014,(4):11-12

目前关于猪疾病的相关研究国内外已不少,其中猪内脏病变的研究也很深入,但是关于屠宰生猪肺脏眼观病变的研究还相对较少。鉴于屠宰生猪肺脏检疫在生猪屠宰检疫工作中的重要性,通过对福州地区某生猪屠宰场大量屠宰生猪肺脏眼观病变的观察,然后进行分类统计,从而了解该地区屠宰生猪肺脏大致病变类型与数量,为该地区疫病的分析起辅助作用,也为屠宰场的脏器检疫工作提供技术支持。  相似文献   

广西主要动物源性产品中磺胺二甲嘧啶残留调查与分析     

马玲  陈凤莲  凌丹  李志源  骆永泉  李丹  张怡轩  吴健敏 《中国畜牧兽医》2018,45(11):3286-3295

为了解广西动物源性产品中磺胺二甲嘧啶（SM₂）残留情况，本研究采用磺胺二甲嘧啶化学发光酶免疫分析法（SM₂-CLEIA）对2013-2017年采集的4 637份动物源性产品进行检测，共检出超标样品122份，涉及鲜乳、猪肉、水产品和猪尿，其中超标猪尿92份，SM₂含量100.51~860.44 μg/kg，猪尿中抗生素残留会污染水体、土壤，再通过动植物的富集作用进入食物链，危害人类健康，应引起重视；而本次受检的水牛奶、鸡肝脏、鸭肝脏、鸭肉均合格。所有受检样品超标率逐年下降，同时规模化养殖场样品合格率高于农村散养户、超市高于农贸市场。不同年份超标样品含量以2014年最高。采用食品安全指数（IFS）对广西动物源性食品中SM₂残留风险进行评估，结果均远小于1，说明在广西地区，现有的SM₂残留对人体的潜在危害较低。  相似文献   

Carbon storage and carbon density of forest vegetation in Luoyang,Western Henan Province     

WANG Yan-Fang  LIU Ling  LI Zhi-Chao  SHANGGUAN Zhou-Ping 《草业学报》2015,24(10):1

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Ultrastructural impairment of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice     

ZHANG Xu  LIU Ming-ming  CHENG Ming  XU Jin  WANG Shu-he  WANG Hui  REN Ya-li  XIU Rui-juan  WANG Su-xia 《园艺学报》2015,31(8):1520

AIM: To investigate the ultrastructural changes of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice. METHODS: BALB/c mice were randomly divided into diabetic group and control group. The expression of insulin and platelet-endothelial cell adhesion molecule-1 (CD31) in islet microvessels was detected by immunohistochemical staining. The ultrastructural changes of islet β cells and islet microvessels were observed under transmission electron microscope. RESULTS: Compared with control group, the number of islet β cells, ratio of β cells/α cells, average number of secretory granules in β cells and insulin expression area per islet in diabetic group were significantly decreased (P<0.01). Besides, diabetic group had fewer microvessels with lower expression of CD31 (P<0.01). Mitochondria in islet microvascular endothelial cells and pericytes in diabetic group were swelling. The basement membrane of islet microvessels became thicker in diabetic group (P<0.01). CONCLUSION: Islet microvascular endothelial cells were impaired in type 1 diabetic mice.  相似文献   

改良索氏提取装置提取艾叶中的挥发性成分     

孔维松  许永  李晶  刘欣  王晋  黄海涛  杨叶昆  李雪梅  杨光宇 《贵州农业科学》2020,48(9):27-32

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汉中市耐寒桉树引种繁殖试验   总被引：2，自引：0，他引：2  

刘彦伟  马玲  衡保清  秦珊 《陕西林业科技》2014,(4)

桉树是目前世界上发展最快的短轮伐期工业原料林树种,但多数不耐寒,适宜在热带和亚热带地区发展。为了扩大其北移引种,从四川省林科院引种耐寒品种开展抗寒、抗逆性研究,初步能在汉中安全越冬。本文以邓恩桉全光喷雾嫩枝扦插试验为例,介绍了耐寒桉树繁殖技术。试验采用4种基质、吲哚丁酸3个处理、1个对照、3个重复,结果表明:扦插不同基质处理以珍珠岩∶腐质土(4∶6)最好,吲哚丁酸不同浓度处理间差异不显著,以浓度1 000mg·L-1最好,平均生根率最高达70.83%。  相似文献   

云南省昆明市执业兽医考试现状及管理措施     

金卫华  张定华  尹红斌 《中国动物检疫》2020,37(1):32-35

昆明市通过加强考务工作,积极宣传动员,建立长效机制等举措,近年来执业兽医考试工作成效显著,2010—2018年昆明市执业兽医累计报考4 819人,其中(助理)执业兽医师共考取通过1 222人。通过对2010—2018年的考试情况分析发现:昆明市报考人数逐年上升,学历水平不断提升;报考人员职业以动物诊疗机构、学生、非兽医从业人员为主;报考人员通过率以科研教育人员、诊疗机构、学生等居前列。上述情况提示,今后应进一步优化执业兽医注册备案管理,强化行业对执业兽医的重视与认可,完善执业兽医考试和继续教育制度。  相似文献   

基于区块链技术的饲料企业物流体系构建研究     

翟玲 《中国饲料》2021,1(7):143-146

近些年来，饲料行业的发展处于上升趋势，其产量和销售额不断扩大，对企业的物流体系有了更高的要求。物流作为饲料企业运营的重要组成部分，影响着企业的效益和市场竞争力。区块链技术是信息发展的前沿技术，能够优化资源配置，提升饲料企业物流效率，从而增强核心竞争力。本文深入分析了目前我国饲料企业物流体系存在的一些问题，阐述了区块链技术在物流中的应用优势，提出了基于区块链技术的饲料企业物流体系的构建策略，为提升企业市场竞争力和实现长期稳步发展提供参考。 [关键词] 区块链技术| 饲料企业| 物流体系  相似文献