Cell biology. The stress of finding NEMO     

Bartek J  Lukas J 《Science (New York, N.Y.)》2006,311(5764):1110-1111

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Identification of monoclonal antibodies against 2,4-D herbicide by ELISA and DNA sequencing     

Brichta J  Fránek M 《Journal of agricultural and food chemistry》2003,51(21):6091-6097

Seven hybridoma clones, E2/G2, E2/B5, E4/C2, G5/E10, F6/C10, B5/C3, and B7, produced within one fusion experiment in 1991 and the clone E4/C2 originated from 1995 were characterized by sequencing of genes coding for variable domains of the antibodies against 2,4-D herbicide. Amino acid sequences of selected antibodies, deduced from DNA analysis, were confirmed by mass spectrometry. Surprisingly, nucleotide sequence analysis of the clones E2/G2 and E2/B5, producing the most sensitive antibodies, proved to have sequence homology of their variable domains, although the IC(50) values determined for these antibodies 9 years prior to the DNA analysis were 2.0 and 8.2 ng/mL, respectively. The same findings arose from the comparison of the immunochemical to DNA data established for G5/E10, F6/C10, and B5/C3 clones producing antibodies with IC(50) values in the range of 26.3-43.1 ng /mL. The clone E4/C2, originating from the later fusion experiment, did not share nucleotide homology with any of the examined clones. Data obtained by ELISA, immunosensor, and DNA analysis within a 9 year period are discussed with respect to hybridoma stability, methodic artifacts, measurement reliability, and other possible factors influencing the result interpretation.  相似文献   

麦类赤霉病菌菌丝的伸长速度及其温度反应     

吴基日  刘宪虎 《延边大学农学学报》1997,19(1):7-11

供试赤霉病菌２４９个菌株，探讨菌丝的伸长速度和对温度的反应，结果表明：２５℃条件下，菌丝的伸长速度在菌株之间的差异很大，对高温或低温的耐性程度在菌株之间也有很大的差异，缩小菌株数，扩大温度梯度所做了试验结果表明：菌株之间对高温和低温耐性、温度反应、最适菌丝伸长温度等都有很大的差异。  相似文献   

A first inference of the phylogeography of the worldwide invader Xylosandrus compactus     

Urvois  T.  Perrier  C.  Roques  A.  Sauné  L.  Courtin  C.  Li  Y.  Johnson  A. J.  Hulcr  J.  Auger-Rozenberg  M.-A.  Kerdelhué  C. 《Journal of pest science》2022,95(3):1217-1231

Native to Southeastern Asia, the ambrosia beetle Xylosandrus compactus is invasive worldwide. Its invasion is favoured by its cryptic lifestyle, symbiosis with a fungus that facilitates a broad range of host plants, and predominant sib-mating reproduction. X. compactus invaded Africa more than a century ago and the Americas and Pacific Islands in the middle of the twentieth century. It was not detected in Europe before 2011, when it was first reported in Italy before quickly spreading to France, Greece and Spain. Despite the negative environmental, agricultural and economic consequences of the invasion of X. compactus, its invasion history and main pathways remain poorly documented. We used COI and RAD sequencing to (i) characterise the worldwide genetic structure of the species, (ii) disentangle the origin(s) of the non-native populations on the three invaded continents and (iii) analyse the genetic diversity and pathways within each invaded region. Three mitochondrial lineages were identified in the native range. Populations invading Europe and the American-Pacific region originated from the first lineage and were only slightly genetically differentiated at nuclear SNP markers, suggesting independent introductions from close sources in or near Shanghai, ca. 60 years apart. Populations invading Africa originated from the second lineage, likely from India or Vietnam.

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SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression     

Pavlova B  Volf J  Ondrackova P  Matiasovic J  Stepanova H  Crhanova M  Karasova D  Faldyna M  Rychlik I 《Veterinary research》2011,42(1):16

ABSTRACT: Genes localized at Salmonella pathogenicity island-1 (SPI-1) are involved in Salmonella enterica invasion of host non-professional phagocytes. Interestingly, in macrophages, SPI-1-encoded proteins, in addition to invasion, induce cell death via activation of caspase-1 which also cleaves proIL-1β and proIL-18, precursors of 2 proinflammatory cytokines. In this study we were therefore interested in whether SPI-1-encoded type III secretion system (T3SS) may influence proinflammatory response of macrophages. To test this hypothesis, we infected primary porcine alveolar macrophages with wild-type S. Typhimurium and S. Enteritidis and their isogenic SPI-1 deletion mutants. ΔSPI1 mutants of both serovars invaded approx. 5 times less efficiently than the wild-type strains and despite this, macrophages responded to the infection with ΔSPI1 mutants by increased expression of proinflammatory cytokines IL-1β, IL-8, TNFα, IL-23α and GM-CSF. Identical macrophage responses to that induced by the ΔSPI1 mutants were also observed to the infection with sipB but not the sipA mutant. The hilA mutant exhibited an intermediate phenotype between the ΔSPI1 mutant and the wild-type S. Enteritidis. Our results showed that the SPI-1-encoded T3SS is required not only for cell invasion but in macrophages also for the suppression of early proinflammatory cytokine expression.  相似文献   

Influence of the lipopolysaccharide structure of Salmonella enterica serovar Enteritidis on interactions with pig neutrophils     

Matiasovic J  Stepanova H  Volf J  Kubala L  Ovesna P  Rychlik I  Faldyna M 《Veterinary microbiology》2011,150(1-2):167-172

The key process for immune response development is the recognition of bacteria by the immune system of the host based on the sensing of pathogen-associated molecular patterns (PAMP). One of the most important PAMP is the lipopolysaccharide (LPS) molecule, a complex molecule present in the outer membrane of Gram negative bacteria. In this study we were interested in how different parts of the LPS of Salmonella enterica serovar Enteritidis are recognized by porcine neutrophils. To this aim, we constructed S. Enteritidis mutants with rfaL and rfaC genes disabled in the attachment of the O-antigen and in the synthesis of the inner oligosaccharide core of LPS, respectively. We found that in the absence of serum, both the rfa mutants associated with neutrophils and stimulated them for reactive oxygen species (ROS) production significantly more than the wild-type strain. Addition of polymyxin B, which neutralized lipid A, the endotoxic moiety of LPS, effectively decreased the association of the wild-type strain and the rfaC mutant with neutrophils, but not the rfaL mutant. This indicates that the oligosaccharide core newly exposed on the surface in the rfaL mutant, protected from interaction in the wild-type strain by the O-antigen but completely absent in the rfaC mutant, may represent a new ligand for porcine neutrophils that cannot be neutralized by polymyxin B.  相似文献   

大麦对赤霉病抗性遗传的研究     

吴基日  刘宪虎  许明子  李艳茹 《延边大学农学学报》1996,(2)

对二棱大麦６个品种之间进行完全双列杂交，分析大麦抗赤霉病的遗传方式．结果表明，大麦抗赤霉病是作用相加的很多基因起作用的数量性状遗传，这些基因之间不存在上位性，只存在显隐性关系，抗性强的基因是显性．所计算的广义遗传力为０．６０，狭义遗传力为０．４５．一部分品种中存在母性效应．  相似文献   

驼乳和牛乳混合干酪的研制     

王娟  何俊霞  潘蕾  王朝霞  李建美  贾志玲  吉日木图 《乳业科学与技术》2010,33(5):205-211

研究了驼乳和牛乳混合干酪的最佳配方,及成熟期间理化和微生物指标的变化。最佳工艺配方为：牛乳的添加比例为40%,发酵剂的添加量为0.06g/L,CaC12的添加量为0.08g/L,凝乳酶的添加量为0.24g/L,凝乳温度为35℃,凝乳pH为6.1。混合干酪成熟期间,蛋白质、脂肪、乳糖、水分含量降低;硬度、咀嚼性增强,黏着性和弹性下降;pH4.6-SN、12%TCA-SN和5%PTA-SN的含量均有不同程度的上升;发酵剂乳酸菌数逐渐下降,非发酵剂活菌数逐渐升高。  相似文献   

Alcalase treatment for elimination of stickiness in pikeperch (Sander lucioperca L.) eggs under controlled conditions          下载免费PDF全文

Jiri Kristan  Miroslav Blecha  Tomas Policar 《Aquaculture Research》2016,47(12):3998-4003

Elimination of egg stickiness is an important factor in artificial reproduction of pikeperch (Sander lucioperca L.). This study was conducted to evaluate the effectiveness of Alcalase enzyme to remove the adhesive layer of pikeperch eggs. The eggs were treated with Alcalase at 0.5, 1.0, 1.5, 2.0 and 5.0 mL L^?1 or a milk/talc solution 2 min post insemination. Duration of exposure was 2 min in Alcalase and 60 min in milk/talc. The highest, albeit not significant, hatching rate (85.4%) was found with 1.5 mL L^?1 of Alcalase, but hatching rates were similar in 0.5, 1.0 and 2.0 mL L^?1. Hatching rates were significantly lower groups treated with 5.0 mL L^?1 Alcalase enzyme (56.4%) compared to groups treated with milk powder and talc (61.3%). Nominally complete removal of adhesiveness was observed in 1.5 and 2 mL L^?1. All Alcalase treatments led to significantly lower incubation duration compared with the traditional milk/talc treatment. The application of Alcalase successfully eliminated pikeperch egg stickiness in less time than with traditional milk/clay/talc methods.  相似文献   

Sorption properties of iron impregnated activated carbon web for removal of methylene blue from aqueous media     

Salman Naeem  Vijay Baheti  Jiri Militky  Jakub Wiener  Promoda Behera  Azeem Ashraf 《Fibers and Polymers》2016,17(8):1245-1255

In this study, impregnation of iron chloride was carried out on needle punched web of waste acrylic fibers, which was subsequently carbonized under layer of charcoal by physical activation in high temperature furnace to produce iron impregnated activated carbon (FeAC). For comparison purpose, one more sample of activated carbon (AC) was prepared without impregnation of iron chloride. Both the webs were carbonized at 1200 °C with no holding time, and characterization of BET surface area, SEM morphology, EDX elemental analysis, XRD crystalline structure was performed. The FeAC web was used as adsorbent for the removal of methylene blue from aqueous solution. The dye removal percentage was investigated at different experimental parameters like different dye concentrations, adsorbent dosage, stirring speed and different pH. The obtained results were analyzed using linear and non-linear forms of Langmuir and Freundlich isotherms and adsorption kinetics (i.e. pseudo first order and pseudo second order model).  相似文献