Comparison of oil and phytosterol levels in germplasm accessions of corn, teosinte, and Job's tears     

Moreau RA  Singh V  Hicks KB 《Journal of agricultural and food chemistry》2001,49(8):3793-3795

Seeds of 49 accessions of corn (Zea mays ssp. mays), 9 accessions of teosinte (Zea species that are thought to be ancestors and probable progenitors to corn), and 3 accessions of Job's tears (Coix lacryma), obtained from a germplasm repository, were ground and extracted with hexane. Whole kernel oil yields and levels of four phytonutrients (free phytosterols, fatty acyl phytosterol esters, ferulate phytosterol esters, and gamma-tocopherol) in the oils were measured. Among the seeds tested, oil yields ranged from 2.19 to 4.83 wt %, the levels of ferulate phytosterol esters in the oil ranged from 0.047 to 0.839 wt %, the levels of free phytosterols in the oil ranged from 0.54 to 1.28 wt %, the levels of phytosterol fatty acyl esters in the oil ranged from 0.76 to 3.09 wt %, the levels of total phytosterols in the oil ranged from 1.40 to 4.38 wt %, and the levels of gamma-tocopherol in the oil ranged from 0.023 to 0.127 wt %. In general, higher levels of all three phytosterol classes were observed in seed oils from accessions of Zea mays ssp. mays than in seed oils from accessions of the other taxonomic groups. The highest levels of gamma-tocopherol were observed in teosinte accessions.  相似文献   

First report of a geminivirus causing leaf curl disease on Trigonella corniculata evidenced by PCR using degenerate primers     

S. K. Raj  S. K. Pandey  G. Chandra  B. P. Singh  R. K. Gupta 《EPPO Bulletin》2001,31(1):115-117

A leaf curl and mottle disease was observed naturally affecting plants of Trigonella corniculata. A geminivirus was detected in infected plants by PCR using degenerate geminivirus primers and by Southern hybridization with a probe specific to tomato leaf curl begomovirus. This is the first report of a member of the Geminiviridae naturally occurring in T. corniculata.  相似文献   

Ovipositional response ofHelicoverpa armigera to different cotton genotypes     

N. S. Butter  Surjit Singh 《Phytoparasitica》1996,24(2):97-102

The ovipositional response ofHelicoverpa armigera (Hübner) to different cotton varieties and species was studied under caged conditions. Maximum oviposition (51.6 eggs/female) was recorded on LH 900, a variety ofGossypium hirsutum, and minimum oviposition (3.0 eggs/female) on G 27, a variety ofG. arboreum. Oviposition, in general, was low (3 to 6.5 eggs/female) onarboreum cottons as compared withhirsutum. Of the number of factors found to affect the oviposition ofH. armigera, the trichome length on the upper surface of the leaf, rather than the density, was positively correlated (r = 0.687*). Among different months in the cropping season, oviposition was maximum (552.7) during April. Oviposition was higher on leaves than on other plant parts.  相似文献   

Purification and Biological Characterization of Host-Specific SV-Toxins from Stemphylium vesicarium Causing Brown Spot of European Pear     

Singh P  Bugiani R  Cavanni P  Nakajima H  Kodama M  Otani H  Kohmoto K 《Phytopathology》1999,89(10):947-953

ABSTRACT Culture filtrates of a pathogenic isolate (IT37) of Stemphylium vesicarium, causing brown spot of European pear, induced veinal necrosis only on pear leaves susceptible to the pathogen. Two host-specific toxins, SV-toxins I and II, were purified from culture filtrates of IT37 by successively using Amberlite XAD-2 resin adsorption, cellulose thin-layer chromatography, and high-performance liquid chromatography under three different sets of conditions. Susceptible cultivars showed veinal necrosis at a SV-toxin I concentration of 0.01 to 0.1 mug/ml, whereas resistant cultivars were insensitive to the toxin at 1,000 mug/ml. SV-toxins I and II caused a dose-dependent increase in electrolyte loss from susceptible leaf tissues. No increase in electrolyte loss was detected in leaf tissues from resistant cultivars. The results of physiological studies indicated that SV-toxins appear to have an early effect on plasma membranes of susceptible leaves. Spores of a nonpathogenic isolate induced necrotic lesions on susceptible leaves in the presence of a small amount of toxin. SV-toxins were detected in intercellular fluids obtained from diseased leaves after inoculation with the pathogen. The results indicate that SV-toxins I and II produced by S. vesicarium can be characterized as host-specific toxins.  相似文献   

Effect of epidermal growth factor on the cumulus expansion, meiotic maturation and development of buffalo oocytes in vitro     

Chauhan MS  Singla SK  Palta P  Manik RS  Madan ML 《The Veterinary record》1999,144(10):266-267

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Cell culture propagated fowl pox vaccine     

M P Singh  K C Saraswat  M P Bansal 《The Veterinary record》1973,92(10):260-261

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A fatal cutaneous granuloma due to Entomophthora coronata in a mare   总被引：2，自引：0，他引：2  

H V Chauhan  G L Sharma  D S Kalra  F C Malhotra  M P Kapur 《The Veterinary record》1973,92(16):425-427

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Experimental reproduction of gangrenous syndrome in buffaloes (Bos bubalus)     

M S Kwatra  A Singh 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1973,20(6):481-489

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The Pattern of Enteroviral Infections in a Herd of Swine          下载免费PDF全文

K. V. Singh  E. H. Bohl 《Canadian journal of veterinary research》1972,36(3):243-248

Twenty-six pigs from four litters in a healthy herd of swine were examined periodically for the fecal excretion of viruses by the use of porcine kidney cell cultures. Viruses were initially isolated from all pigs between 34 to 64 days of age. The pigs within each litter began shedding virus in their feces approximately at the same time, usually within one week, and the type of virus initially recovered was usually the same. Subsequently, waves of infection with different enteroviruses appeared to occur during the observation period of six months. At least six antigenically different viruses were isolated from this herd over a 26-month period. Most, if not all, of these viruses were considered to belong to the enterovirus group. No disease was associated with these enteroviral infections.

The colostrum and milk of sows contained significant amounts of enteroviral antibodies. Prior to nursing, the serum of new-born pig contained no enteroviral antibodies but, shortly after nursing, high titers of such antibodies were present in the serum. Antibodies were detected in the feces of suckling pigs.

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Studies on protein metabolism in adult sheep. I. Endogenous urinary nitrogen determined graphically and by the direct method based on nitrogen free ration     

M Singh  V Mahadevan 《The Indian veterinary journal》1968,45(8):659-665

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