全文获取类型
收费全文 | 466篇 |
免费 | 24篇 |
专业分类
林业 | 37篇 |
农学 | 4篇 |
基础科学 | 7篇 |
94篇 | |
综合类 | 73篇 |
农作物 | 5篇 |
水产渔业 | 41篇 |
畜牧兽医 | 180篇 |
园艺 | 24篇 |
植物保护 | 25篇 |
出版年
2024年 | 3篇 |
2021年 | 7篇 |
2020年 | 6篇 |
2019年 | 15篇 |
2018年 | 7篇 |
2017年 | 9篇 |
2016年 | 7篇 |
2015年 | 8篇 |
2014年 | 6篇 |
2013年 | 24篇 |
2012年 | 25篇 |
2011年 | 38篇 |
2010年 | 16篇 |
2009年 | 14篇 |
2008年 | 27篇 |
2007年 | 28篇 |
2006年 | 24篇 |
2005年 | 35篇 |
2004年 | 30篇 |
2003年 | 21篇 |
2002年 | 22篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1998年 | 5篇 |
1997年 | 5篇 |
1995年 | 7篇 |
1994年 | 4篇 |
1993年 | 6篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1989年 | 2篇 |
1987年 | 3篇 |
1985年 | 2篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1973年 | 2篇 |
1972年 | 3篇 |
1970年 | 2篇 |
1966年 | 3篇 |
1965年 | 2篇 |
1964年 | 3篇 |
1954年 | 2篇 |
1907年 | 2篇 |
1896年 | 2篇 |
1893年 | 2篇 |
排序方式: 共有490条查询结果,搜索用时 31 毫秒
31.
Muscadine grapes have unique aroma and flavor characteristics. Although a few studies reported high polyphenols content of muscadine grapes, little research has been conducted to evaluate the phenolic compounds bioactivities in any muscadine grape cultivar. The objective of this study was to evaluate the effect of phenolic compounds in muscadine grapes on cancer cell viability and apoptosis. Four cultivars of muscadine (Carlos, Ison, Noble, and Supreme) were assessed in this study. Phenolic compounds were extracted from muscadine skins and further separated into phenolic acids, tannins, flavonols, and anthocyanins using HLB cartridge and LH20 column. Some individual phenolic acids and flavonoids were identified by HPLC. Anthocyanin fractions were more than 90% pure. The effect of different fractions on the viability and apoptosis of two colon cancer cell lines (HT-29 and Caco-2) was evaluated. A 50% inhibition of cancer cell population growth for the two cell lines was observed at concentrations of 1-7 mg/mL for crude extracts. The phenolic acid fractions showed a 50% inhibition at the level of 0.5-3 mg/mL. The greatest inhibitory activity was found in the anthocyanin fraction, with a 50% inhibition at concentrations of approximately 200 microg/mL in HT-29 and 100-300 microg/mL in Caco-2. Anthocyanin fractions also resulted in 2-4 times increase in DNA fragmentation, indicating the induction of apoptosis. These findings suggest that polyphenols from muscadine grapes may have anticancer properties. 相似文献
32.
An important consequence of acidification is the mobilization of Al from the edaphic to the aquatic environment. Elevated Al levels in acidic waters may be toxic to fish. Eggs, larvae, and postlarvae of white suckers (Catostomus commersoni) and brook trout (Salvelinus fontinalis) were exposed in laboratory bioassays to pH levels 4.2 to 5.6 and inorganic Al concentrations of 0 to 0.5 mg l?1. Aluminum toxicity varied with both pH and life history stage. At low pH levels (4.2 to 4.8), the presence of Al (up 0.2 mg l?1 for white suckers; 0.5 mg l?1 for brook trout) was beneficial to egg survival through the eyed stage. In contrast, Al concentrations of 0.1 mg l?1 (for white suckers) or 0.2 mg l?1 (for brook trout) and greater resulted in measurable reductions in survival and growth of larvae and postlarvae at all pH levels (4.2 to 5.6). Aluminum was most toxic in over-saturated solutions at pH levels 5.2 to 5.4. The simultaneous increase in Al concentration with elevated acidity must be considered to accurately assess the potential effect of acidification of surface waters on survival of fish populations. 相似文献
33.
34.
Romero MP Tovar MJ Girona J Motilva MJ 《Journal of agricultural and food chemistry》2002,50(19):5349-5354
The HPLC phenolic profile of virgin olive oils obtained from young olive trees (Arbequina cv.) grown under different deficit irrigation strategies was studied. Deficit irrigation (RDI) did not affect all the phenolic compounds in the same way. Lignans, vanillic acid, vanillin, and the unknown phenolic compound named P24 increased in the oils from the most irrigated treatments. The secoiridoid derivatives and the unknown phenolic compound named P19 increased in the oils from the most stressed irrigation treatments. The period of growth where a water stress significantly affects the phenolic profile of oils was between pit hardening and the first stages of fruit growth and oil accumulation, independently of the water applied during the previous period to harvest. The phenolic profile and those parameters related to phenol content, oxidative stability, and the bitter index were significantly affected only in the most severe RDI strategies. Other strategies produced important savings in irrigation requirements and an increase in the water use efficiency without noticeably affecting the phenolic profile. 相似文献
35.
Matti J. Salmela Stephen Cavers Joan E. Cottrell 《Forest Ecology and Management》2011,262(6):1020-1029
Environment-driven genetic differentiation among populations is a common feature among forest trees, and an understanding of how populations have adapted to their home site conditions is essential for management and conservation practices. In Scotland, 84 native Scots pine (Pinus sylvestris L.) woodlands are recognised by the Forestry Commission and they occupy highly diverse environments from the maritime west coast to continental sites in eastern Scotland. However, it is not known whether adaptations to local environments along sharp temperature and rainfall gradients have occurred in different populations and as a result, the seed transfer guidelines of the species are based only on data from isozymes and monoterpenes. In this study of an outdoor common-garden trial, we used chlorophyll fluorescence to examine whether seedlings from 32 open-pollinated families and eight populations from sites experiencing contrasting annual temperature regimes differed in their response to variation in natural outdoor temperatures between September 2009 and May 2010. In addition, growth initiation in spring was recorded. Photochemical capacity at photosystem II Fv/Fm showed a distinct seasonal trend and remained at relatively high levels (∼0.7) until November. Following a period of over 2 weeks with temperatures below or close to 0 °C, Fv/Fm started decreasing towards its minimum values recorded in early March when population means varied between 0.35 and 0.45. By early May and along with rising temperatures, photochemical capacity had recovered to the same level as observed in early November. Populations were found to respond differently to the cold period starting in December. The largest drop in photochemical capacity was observed in seedlings from a low-altitude population located in the maritime western Scotland, while in seedlings from higher-altitude locations in the cooler eastern Scotland, the response was smaller. In March, the recovery of photochemical capacity was slowest in seedlings from the mildest and coolest sites. Evidence of adaptive genetic differentiation was also found in spring phenology. Initiation of shoot elongation and needle flush were earlier in families from higher altitudes (cooler areas), but population differences were not significant at the α = 0.05 level. These results suggest that adaptation to the spatially heterogeneous environment in Scotland has taken place in Scots pine and that in order to minimise the risk of planting maladapted seed stock, the patterns of environmental and adaptive genetic variation should be taken into account in the management of genetic resources in this species. 相似文献
36.
Mariangela Hungria Joan M. Ellis Ralph W. F. Hardy Allan R. J. Eaglesham 《Biology and Fertility of Soils》1993,15(3):208-214
Summary Bradyrhizobial strain BTAi 1 nodulates both stems and roots of Aeschynomene spp. Previous work has shown that it contains bacteriochlorophyll a and forms photosynthetic reaction centers, and has provided indirect evidence of photosynthesis by bacteroids within stem nodules. Here we report physiological and biochemical characteristics of BTAi 1 ex planta, which also suggest the presence of photosynthetic activity. Light-stimulated uptake of 14CO2 by BTAi 1 was detected at all stages of growth. Inhibitors of photosynthesis, 1,10-orthophenanthroline and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), and the uncoupler NH4Cl, immediately suppressed light-driven 14CO2 uptake and increased O2 uptake. BTAi 1 is strictly aerobic and was unable to grow without organic C even in the light; also, it was unable to grow chemoautotrophically in an atmosphere enriched with H2 and CO2. In micro-aerobic conditions, strain BTAi 1 expressed acetylene reducing activity ex planta in an N-free medium. The highest rates of light-stimulated 14CO2 uptake and acetylene-reducing activity occurred during the exponential and early stationary phases of growth. Acetylene-reducing rates at a low glucose concentration were increased following a light-dark cycle in comparison with continuous dark conditions. 相似文献
37.
Rogers AE Cappallo RJ Hinteregger HF Levine JI Nesman EF Webber JC Whitney AR Clark TA Ma C Ryan J Corey BE Counselman CC Herring TA Shapiro II Knight CA Shaffer DB Vandenberg NR Lacasse R Mauzy R Rayhrer B Schupler BR Pigg JC 《Science (New York, N.Y.)》1983,219(4580):51-54
The Mark III very-long-baseline interferometry (VLBI) system allows recording and later processing of up to 112 megabits per second from each radio telescope of an interferometer array. For astrometric and geodetic measurements, signals from two radio-frequency bands (2.2 to 2.3 and 8.2 to 8.6 gigahertz) are sampled and recorded simultaneously at all antenna sites. From these dual-band recordings the relative group delays of signals arriving at each pair of sites can be corrected for the contributions due to the ionosphere. For many radio sources for which the signals are sufficiently intense, these group delays can be determined with uncertainties under 50 picoseconds. Relative positions of widely separated antennas and celestial coordinates of radio sources have been determined from such measurements with 1 standard deviation uncertainties of about 5 centimeters and 3 milliseconds of arc, respectively. Sample results are given for the lengths of baselines between three antennas in the United States and three in Europe as well as for the arc lengths between the positions of six extragalactic radio sources. There is no significant evidence of change in any of these quantities. For mapping the brightness distribution of such compact radio sources, signals of a given polarization, or of pairs of orthogonal polarizations, can be recorded in up to 28 contiguous bands each nearly 2 megahertz wide. The ability to record large bandwidths and to link together many large radio telescopes allows detection and study of compact sources with flux densities under 1 millijansky. 相似文献
38.
Stone EC Cummings AC McDonald FB Heikkila BC Lal N Webber WR 《Science (New York, N.Y.)》2005,309(5743):2017-2020
Voyager 1 crossed the termination shock of the supersonic flow of the solar wind on 16 December 2004 at a distance of 94.01 astronomical units from the Sun, becoming the first spacecraft to begin exploring the heliosheath, the outermost layer of the heliosphere. The shock is a steady source of low-energy protons with an energy spectrum approximately E(-1.41 +/- 0.15) from 0.5 to approximately 3.5 megaelectron volts, consistent with a weak termination shock having a solar wind velocity jump ratio r=2.6(-0.2)(+0.4). However, in contradiction to many predictions, the intensity of anomalous cosmic ray (ACR) helium did not peak at the shock, indicating that the ACR source is not in the shock region local to Voyager 1. The intensities of approximately 10-megaelectron volt electrons, ACRs, and galactic cosmic rays have steadily increased since late 2004 as the effects of solar modulation have decreased. 相似文献
39.
40.
Mao JH Kim IJ Wu D Climent J Kang HC DelRosario R Balmain A 《Science (New York, N.Y.)》2008,321(5895):1499-1502
The enzyme mTOR (mammalian target of rapamycin) is a major target for therapeutic intervention to treat many human diseases, including cancer, but very little is known about the processes that control levels of mTOR protein. Here, we show that mTOR is targeted for ubiquitination and consequent degradation by binding to the tumor suppressor protein FBXW7. Human breast cancer cell lines and primary tumors showed a reciprocal relation between loss of FBXW7 and deletion or mutation of PTEN (phosphatase and tensin homolog), which also activates mTOR. Tumor cell lines harboring deletions or mutations in FBXW7 are particularly sensitive to rapamycin treatment, which suggests that loss of FBXW7 may be a biomarker for human cancers susceptible to treatment with inhibitors of the mTOR pathway. 相似文献