全文获取类型
收费全文 | 179篇 |
免费 | 14篇 |
国内免费 | 1篇 |
专业分类
林业 | 9篇 |
农学 | 4篇 |
34篇 | |
综合类 | 19篇 |
农作物 | 9篇 |
水产渔业 | 7篇 |
畜牧兽医 | 103篇 |
植物保护 | 9篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2020年 | 6篇 |
2019年 | 6篇 |
2018年 | 4篇 |
2017年 | 4篇 |
2016年 | 3篇 |
2015年 | 5篇 |
2014年 | 4篇 |
2013年 | 9篇 |
2012年 | 9篇 |
2011年 | 7篇 |
2010年 | 5篇 |
2009年 | 6篇 |
2008年 | 15篇 |
2007年 | 13篇 |
2006年 | 7篇 |
2004年 | 7篇 |
2003年 | 8篇 |
2002年 | 6篇 |
2001年 | 5篇 |
2000年 | 9篇 |
1999年 | 1篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1994年 | 1篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 3篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1977年 | 2篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1972年 | 3篇 |
1971年 | 3篇 |
1970年 | 4篇 |
1969年 | 4篇 |
1967年 | 2篇 |
1966年 | 2篇 |
排序方式: 共有194条查询结果,搜索用时 15 毫秒
11.
Transplantation: pairing of donor and recipient 总被引:5,自引:0,他引:5
F H Bach 《Science (New York, N.Y.)》1970,168(936):1170-1179
12.
Cell mediated immunity: separation of cells involved in recognitive and destructive phases 总被引:43,自引:0,他引:43
F H Bach M Segall K S Zier P M Sondel B J Alter M L Bach 《Science (New York, N.Y.)》1973,180(84):403-406
The mixed leukocyte culture (MLC) and the cell mediated lympholysis (CML) assays are used as in vitro models of the afferent, or recognitive, and efferent, or destructive, phases of the homograft reaction. Activity in both of these tests has been related to differences at the major histocompatibility complex, HL-A in man and H-2 in mouse. Recent evidence suggests that the presumed cell surface differences which lead to cell proliferation in MLC are different from those which act as a target for CML. Data are presented providing further support for this hypothesis; in addition separate cell populations may respond to the differences which activate cells in MLC and to the differences which serve as targets for CML. There thus appears to be a dichotomy both for genetic control of, and cell populations involved in, the recognitive and destructive phases of cell mediated immunity. 相似文献
13.
Genetic and immunological complexity of major histocompatibility regions 总被引:24,自引:0,他引:24
There are genetic differences within the major histocompatibility complex of the mouse which lead to skin graft rejection but which cannot be detected serologically. When confronted with these differences on allogeneic cells, lymphocytes proliferate in vitro. In other cases, in vitro lymphocyte proliferation but no skin graft rejection is associated with loci that are linked to but genetically separable from the loci controlling the serologically defined antigens. 相似文献
14.
One-way stimulation in mixed leukocyte cultures 总被引:67,自引:0,他引:67
We have developed an improved method for the mixed leukocyte culture test. Control values, as determined by rates of incorporation of thymidine, are very low, allowing evaluation of low levels of stimulation in homologous cell mixtures. One-way stimulation is assayed by treating the cells of one individual with mitomycin C; treated cells cannot respond (incorporate thymidine) but can still stimulate homologous untreated cells to do so. 相似文献
15.
桃果皮毛、果肉颜色对果实糖与酸含量的影响及相关性研究 总被引:8,自引:0,他引:8
对山桃和栽培桃品种杂交后代 10 7个个体 (分成 2 2个毛桃和 85个油桃 ,或 75个白肉桃和 3 2个黄肉桃 )的果实糖酸含量遗传规律进行了研究。结果表明 ,桃果实中的可溶性糖和酸主要是蔗糖和苹果酸 ,分别占总可溶性糖的 65%左右和总酸含量的 50 %。油桃各基因型的蔗糖、果糖、山梨糖醇、总糖、可溶性固形物及奎宁酸平均含量均显著高于毛桃 ,而毛桃和油桃基因型之间的苹果酸、柠檬酸、总酸平均含量及糖酸比没有显著差异。所有毛桃和油桃基因型的莽草酸含量都很低 ,为 0 .0 11~ 0 .0 46meq·10 0g- 1FW。果实内葡萄糖和果糖含量呈正相关 (r =0 .63 ) ,蔗糖、葡萄糖、果糖分别与山梨糖醇呈显著或极显著直线正相关 ,且苹果酸与蔗糖、山梨糖醇、总糖和可溶性固形物呈显著正相关。毛桃的平均单果重显著大于油桃 ,且可溶性固形物和总酸含量与果实的大小呈显著直线负相关。此外 ,盛花后果实发育天数影响果实内糖酸含量 相似文献
16.
Gougeon RD Soulard M Miehé-Brendlé J Chézeau JM Le Dred R Jeandet P Marchal R 《Journal of agricultural and food chemistry》2003,51(14):4096-4100
Two natural calcium-rich bentonites used for the removal of wine proteins, originating from Greece and Turkey, were studied by X-ray diffraction, thermal analysis, and solid state NMR, before and after activation by solid Na(2)CO(3). Exchange of Ca(2+) by Na(+) mainly occurs for cations located at the edge of layers and only weakly for interlayer cations. This Na(2)CO(3) activation process leads to an increased efficiency in the adsorption process of bovine serum albumin (BSA) used as a model protein for both bentonites. A direct correlation is observed between the extent of Ca(2+)/Na(+) exchange, the strength of adsorption of BSA, and the extent of unfolding of BSA backbone. 相似文献
17.
W Busch J D Klinsky R Slucka S Bach 《Archiv fuer experimentelle veterinaermedizin》1977,31(6):799-808
Reference is made to the authors' own experience and to evaluated literature in an account of possibilities which so far have been explored for planned control of reproduction processes in industrialised cattle production. Synchronisation of oestrus in heifer has been found to be suitable for reproduction control even under conditions of industrialised production. The method has been in use on several farms. Synchronisation of oestrus in cow is an approach which still is in the process of translation into practice. Obtainable advantages, like those in the context of heifer, will include better job conditions. Systematic puerperal monitoring, too, will yield positive effects in controlling post-partum readiness of cow for reproduction. Results so far achieved include progress towards the introduction of a complex reproduction technology in cattle production. Before such complete system of reproduction control can be used, further studies are required into some fundamental aspects of the problem, including calving control, pregnancy examination, and gravidity hagiene. 相似文献
18.
Argüelles D Carmona JU Pastor J Iborra A Viñals L Martínez P Bach E Prades M 《Research in veterinary science》2006,81(2):237-245
The aim of this study was to evaluate single and double centrifugation tube methods for concentrating equine platelets. Whole blood samples were collected from clinically normal horses and processed by use of single and double centrifugation tube methods to obtain four platelet concentrates (PCs): PC-A, PC-B, PC-C, and PC-D, which were analyzed using a flow cytometry hematology system for hemogram and additional platelet parameters (mean platelet volume, platelet distribution width, mean platelet component concentration, mean platelet component distribution width). Concentrations of transforming growth factor beta 1 (TGF-beta(1)) were determined in all the samples. Platelet concentrations for PC-A, PC-B, PC-C, and PC-D were 45%, 44%, 71%, and 21% higher, respectively, compared to the same values for citrated whole blood samples. TGF-beta(1) concentrations for PC-A, PC-B, PC-C, and PC-D were 38%, 44%, 44%, and 37% higher, respectively, compared to citrated whole blood sample values. In conclusion, the single and double centrifugation tube methods are reliable methods for concentrating equine platelets and for obtaining potentially therapeutic TGF-beta(1) levels. 相似文献
19.
Bach JF Rozanski EA MacGregor J Betkowski JM Rush JE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(5):1132-1135
Pulmonary arterial hypertension (PH) is a pathologic condition in dogs characterized by abnormally high pressures in the pulmonary circulation and has been associated with a poor outcome. Sildenafil is a type V phosphodiesterase inhibitor that produces nitric oxide mediated vasodilatation. Sildenafil treatment decreases pulmonary arterial pressure and pulmonary vascular resistance in people with PH. The purpose of this study was to describe the clinical characteristics and outcome of dogs with PH treated with sildenafil. The cardiology database was searched for dogs with PH treated with sildenafil. PH was defined as systolic pulmonary arterial pressure (PAPs) > or = 25 mmHg at rest. Medical records were reviewed for the following information: signalment, duration and type of clinical signs before treatment, underlying disease, estimated or measured PAPs, dosage and dosing interval of sildenafil, and the effect of treatment on clinical signs and pulmonary arterial pressure and survival time. Thirteen affected dogs were identified. Clinical signs included collapse, syncope, respiratory distress, and cough. Duration of clinical signs before presentation ranged from 3 days to 5 months. An underlying cause was identified in 8 dogs. The median sildenafil dosage was 1.9 mg/kg. Ten dogs received concurrent medications. Median PAPs was 90 mmHg; 8 dogs were reevaluated after therapy, and the median decrease in PAPs was 16.5 mmHg. The median survival time of all dogs was 91 days. Sildenafil appeared to be well tolerated in dogs with PH and was associated with decreased PAPs and amelioration of clinical signs in most. Sildenafil represents a reasonable treatment option for dogs with pulmonary hypertension. 相似文献
20.
Jensen MB Pedersen LJ Theil PK Yde CC Bach Knudsen KE 《Journal of animal science》2012,90(6):1910-1919
The present study investigated the effects of source and level of dietary fiber (DF) and feeding frequency (once vs. twice daily) on feeding motivation and plasma metabolites at 4 different time points post feeding. Sixty pregnant sows (Sus scrofa, 4 blocks of 15 sows) were allocated to 1 of 5 diets within blocks. Four diets were restricted (approximately 35 MJ ME/d): a barley and wheat control diet (171 g DF/kg DM; 12 g DF/MJ ME), and 3 fiber diets formulated to contain 35% DF by including pectin residue (323 g DF/kg DM; 25 g DF/MJ ME), potato pulp (404 g DF/kg DM; 29 g DF/MJ ME), or sugar beet pulp (367 g DF/kg DM; 25 g DF/MJ ME). The fifth diet was a mixture including an equal amount of the 3 fiber diets offered semi ad libitum (ad libitum access to feed during 6 periods of 1 h starting at 0300, 0600, 1100, 1500, 1800, and 2300; 354 g DF/kg DM; 25 g DF/MJ ME). The experimental period included 2 periods of 4 wk each. Restricted-fed sows were fed once daily (0800 h) during the first period and twice daily (0800 and 1500 h) during the second period, or vice versa. Semi ad libitum fed sows had access to feed 6 times a day in both periods. In each period, the feeding motivation was assessed in an operant conditioning test, and samples of peripheral blood were taken in a balanced design, at 0900, 1200, 1900, and 0700 h, corresponding to 1, 4, 11, and 23 h after feeding for restricted sows fed once daily. No differences in the feeding motivation were found between the 4 restricted diets at any of the time points post feeding, but semi ad libitum fed sows had a decreased feeding motivation (P < 0.001). Among the restricted-fed sows, feeding twice daily resulted in decreased feeding motivation at 1900 h (P < 0.001) and at 0700h (P < 0.05) compared with feeding once daily, but not at 0900 and 1200 h, indicating that feeding twice daily reduced feeding motivation during the night compared with feeding once daily. Among restricted-fed sows, plasma concentrations of short-chain fatty acids (SCFA) were greater in sows fed high-fiber diets compared with the control (P = 0.02). Nonesterified fatty acid was least in sows on the control diet and greatest in sows on the potato diet, whereas sows on the pectin and sugar beet diets were intermediate (P < 0.001). Less diurnal variation in glucose (P < 0.001) was seen in sows on high-fiber diets. In spite of the found effects on plasma metabolites, the applied level of fiber in the diet of restrictedly fed sows did not reduce their feeding motivation irrespective of fiber source. 相似文献