首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   8208篇
  免费   419篇
  国内免费   9篇
林业   588篇
农学   275篇
基础科学   62篇
  1387篇
综合类   1234篇
农作物   540篇
水产渔业   647篇
畜牧兽医   3026篇
园艺   215篇
植物保护   662篇
  2023年   46篇
  2022年   69篇
  2021年   138篇
  2020年   151篇
  2019年   174篇
  2018年   154篇
  2017年   148篇
  2016年   173篇
  2015年   130篇
  2014年   184篇
  2013年   345篇
  2012年   409篇
  2011年   562篇
  2010年   320篇
  2009年   299篇
  2008年   548篇
  2007年   502篇
  2006年   512篇
  2005年   539篇
  2004年   442篇
  2003年   480篇
  2002年   421篇
  2001年   92篇
  2000年   87篇
  1999年   102篇
  1998年   86篇
  1997年   79篇
  1996年   67篇
  1995年   89篇
  1994年   86篇
  1993年   73篇
  1992年   56篇
  1991年   54篇
  1990年   63篇
  1989年   45篇
  1988年   41篇
  1987年   35篇
  1986年   48篇
  1985年   50篇
  1984年   49篇
  1983年   46篇
  1982年   32篇
  1981年   25篇
  1980年   34篇
  1979年   29篇
  1978年   37篇
  1977年   37篇
  1976年   35篇
  1975年   38篇
  1974年   30篇
排序方式: 共有8636条查询结果,搜索用时 15 毫秒
101.
Netminder®, a water‐based silicone barrier release coating, was highly effective in reducing biofouling of lantern nets during grow‐out/overwintering of bay scallops, Argopecten irradians irradians, in a large‐scale restoration programme in New York, USA. Compared with untreated nets, scallops held in Netminder®‐coated nets experienced reduced survival after 8 months, probably due to higher loads of epibionts on their shells. However, this was countered by higher reproductive and overall condition of scallops in treated vs. untreated nets; there were no consistent differences in shell growth in different net treatments. Although further testing is recommended under a wider range of conditions, we conclude that for the purposes of our scallop restoration work, coating nets with Netminder® would eliminate the need for a gear change in spring, thus reducing labour costs; the coating process should be refined so that Velcro closures of nets do not become sealed shut.  相似文献   
102.
I summarize the total allowable catch (TAC) fishery management system, which forms part of the European Common Fisheries Policy (CFP) as complicated, inaccurate and ineffective. Total allowable effort (TAE), i.e. limits on fishing capacity and days‐at‐sea, provides a simpler, readily enforceable control to protect all species affected by a fishery. Fishing capacity of vessels in each region‐gear fleet could be capped through limits on engine power, gear, crew, and fittings, and/or reducing allowances of days‐at‐sea for large, powerful vessels. Two basic, fishery‐dependent indicators are put forward to assess sustainability of a fishery dependably even in the absence of fishery‐independent data. One is socio‐economic, the other is ecological. Fishery working groups within each marine region would recommend a TAE to sustain their fishery while also protecting the most critically affected species or processes. Regional working groups would supervise a consistent approach across the different fisheries in their region. A pan‐European group would set policy, implement external agreements, set rules for trading TAE if necessary and harmonize fishing opportunities across regions. To ease negotiations on TAEs with the fishing industry when an ecosystem needs to recover, I propose compensation payments funded by re‐direction of existing CFP subsidies that promote fishing.  相似文献   
103.
Three practical-type diets utilizing fishmeal and casein as the protein sources and containing fish oil (FO), safflower oil (SO) or linseed oil (LO) were fed to duplicate groups of juvenile turbot (Scophthalmus maximus) of initial weight 1.2 g for a period of 12 weeks. No differences in final weight, mortality or development of pathological lesions were evident either between duplicate tanks or between dietary treatments over this period. Fish fed diets containing SO and LO contained significantly greater amounts of liver triacylglycerol compared to fish fed FO. The major C18 polyunsaturated fatty acids (PUFA) in SO and LO diets, 18:2(n-6) and 18:3(n-3) respectively, were readily incorporated into both total lipid and individual phospholipids of turbot tissues. There was no accumulation of the Δ6-desaturation products of these fatty acids, namely 18:3(n-6) and 18:4(n-3), in any of the tissues examined. The products of elongation of 18:2(n-6) and and 18:3(n-3), 20:2(n-6) and 20:3(n-3) respectively, accumulated in both total lipid and phospholipids with the highest levels of 20:2(n-6) in liver PC and 20:3(n-3) in liver PE. Eicosapentaenoic acid [EPA, 20:5(n-3)] levels exceeded those of arachidonic acid [AA, 20:4(n-6)] in phosphatidylinositol (PI) from liver and gill of fish fed LO. EPA levels in liver PI from fish fed LO were 3-fold and 2-fold greater than SO-fed and FO-fed fish, respectively. Fish fed diets containing SO and LO had significantly reduced levels of AA in liver and muscle total lipid and lower AA in individual phospholipid classes of liver and gill compared to FO-fed fish. The concentration of thromboxane B2 was significantly reduced in plasma and isolated gill cells stimulated with calcium ionophore A23187 of fish fed SO and LO compared to those fed FO. Prostaglandin E produced by isolated gill cells stimulated with A23187 was significantly reduced in fish fed both SO and LO compared to fish fed FO.  相似文献   
104.
The estuarine‐dependent brown shrimp, Farfantepenaeus aztecus, is a significant commercial fishery and important species in the Gulf of Mexico (GOM) ecosystem as well as being a key component in energy transfer between benthic and pelagic food web systems. Because of the economical and ecological importance of brown shrimp, we developed a spatial population model to identify places of high shrimp density under a set of spatial, environmental and temporal variables in the Northern Gulf of Mexico (NGOM). We used fisheries‐independent data collected by the Southeast Area Monitoring and Assessment Program (SEAMAP) from 1992 to 2007 (summer and fall seasons). The relationship between the predictor variables and shrimp density was modeled using Boosted Regression Trees (BRT). Within the environmental variables included in the model, bottom type and depth of the water column were the most important predictors of shrimp density in the NGOM. Spatial predictions performed using the trained BRT model for summer and fall seasons showed a spatial segregation of shrimp density. During the summer, higher densities were predicted near the Texas and Louisiana coast and during the fall, higher densities were predicted further offshore. The model performed well and allowed successful prediction of brown shrimp hot spots in the NGOM. Model results allow fisheries managers to evaluate the potential impact from fisheries on the resource and to develop future fisheries management strategies, understand the biology of brown shrimp as well as assess the potential impacts of oil spills or climate change.  相似文献   
105.
Diatoms Thalassiosira pseudonana (Hustedt) Hasle and Heimdal, Chaetoceros calcitrans (Paulsen) Takano and Chaetoceros sp. (Aquaculture Research Corporation), and phytoflagellates Isochrysis galbana Parke, Isochrysis aff. galbana Green (T-Iso) and Tetraselmis suecica (Kylin) Butcher were harvested at exponential and stationary phases of growth and biochemical compositions determined. Moisture content declined in all species at the stationary phase of growth. The siliceous nature of the diatom reduced inherent organic content relative to that of the flagellate. Total pigments were more concentrated in the stationary phases of all species except Tetraselmis. Inclusion of cellular lipid and protein was highest in species of Isochrysis with the carbohydrate constituents of the phytoflagellates presenting a complex mixture of glucose, galactose, mannose, xylose, arabinose, ribose, fucose, and rhamnose. Content of carbohydrates was generally higher in diatoms than phytoflagellates, with glucose the dominant sugar originating from a reserve glucan considered digestible by bivalves. Caloric content of lipid, carbohydrate and protein in phytoplankton allowed for ranking as sources of energy in the following decreasing order: Isochrysis aff. galbana (T-Iso), Isochrysis galbana, Chaetoceros calcitrans, Tetraselmis suecica, Thalassiosira pseudonana, and Chaetoceros sp. (ARC), with all but Thalassiosira exhibiting higher energy levels in the stationary cells.  相似文献   
106.
107.
Methods of assessing extinction risk in marine fishes   总被引:1,自引:0,他引:1  
The decline and disappearance of species from large parts of their former geographical range has become an important issue in fisheries ecology. There is a need to identify which species are at risk of extinction. The available approaches have been subject to considerable debate – particularly when applied to commercially exploited species. Here we have compiled methods that have been used or may be used for assessing threat status of marine organisms. We organize the methods according to the availability of data on the natural history, ecology and population biology of species. There are three general approaches to inferring or assessing extinction risk: (i) correlative approaches based on knowledge of life histories and ecology; (ii) time‐series approaches that examine changes in abundance; and (iii) demographic approaches based on age‐ or stage‐based schedules of vital rates and fisheries reference points. Many methods are well suited to species that are highly catchable and/or have relatively low productivity, but theory is less well developed for assessing extinction risk in species exhibiting narrow geographical distributions or ecological specialization. There is considerable variation in both definitions of extinction risk and the precision and defensibility of the available risk assessment methods, so we suggest a two‐tiered approach for defining and assessing extinction risk. First, simple methods requiring a few easily estimated parameters are used to triage or rapidly assess large numbers of populations and species to identify potentially vulnerable populations or species. Second, the populations and species identified as vulnerable by this process can then be subject to more detailed and rigorous population analysis explicitly considering sources of error and uncertainty.  相似文献   
108.
The direct effects of osmotic pressure (salinity) on growth performance and lipid composition were investigated in fish cells in culture. Cell lines from a relatively stenohaline marine species, turbot (Scophthalmus maximus) (TF) and an anadromous species, Atlantic salmon (AS) were cultured in media supplemented with NaCl to produce osmotic pressures varying from 300 to 500 mOsm kg−1. The growth rates of the two cell lines were affected in a similar manner by the salinity of the media with the rank order for both peak cell numbers and growth rates up to the day of peak cell number being 300 > 350 > 400 > 450 > 500 mOsm kg−1. Cell death occurred in both cell lines in older cultures at all salinities with the greatest loss of viable cells in media of 300 and 350 kg−1. However, there were quantitative and qualitative differences between the cell lines in their lipid metabolism in response to the salinity of the media. The lipid content expressed per cell showed a positive correlation between lipid per cell and salinity in TF cells, but this was less apparent in AS cells. The percentage of total polar lipid classes increased with increasing salinity in TF cells due mainly to graded increases in the percentages of choline phospholipids. In contrast, there were no significant differences in the proportions of polar and neutral lipid classes with salinity in AS cells. The only significant effect of salinity in AS cells was a decreased proportion of dimethylacetals in total lipid at the highest salinity. The same significant effect of salinity on dimethylacetal content of total lipid was observed in TF cells. However, in addition there was a graded decrease in the percentage of 18:2n-9 in TF cell total lipid with increasing salinity. This was accompanied by increased percentages of total n-3 and n-6 PUFA with higher proportions of both groups of PUFA at 450 and 500 compared with 300 mOsm kg−1. The results show that environmental salinity, in the absence of hormonal or other physiological stimuli, has direct effects on the growth and lipid metabolism of fish cells and that these effects differ in cells from different fish species.  相似文献   
109.
A biochemically based model was developed to simulate the growth, development and metamorphosis of larvae of the Pacific oyster, Crassostrea gigas. The model is unique in that it (1) defines larvae in terms of their protein, neutral lipid, polar lipid, carbohydrate and ash content; (2) tracks weight separately from length to follow larval condition index and (3) includes genetic variation in growth efficiency and egg quality to better simulate cohort population dynamics. The model includes parameterizations for larval filtration, ingestion and respiration that determine growth rate and processes controlling larval mortality and metamorphosis. Changes in tissue composition occur as the larva grows and in response to the biochemical composition of the food.

The simulations show that genetically determined variations in growth efficiency produce significant changes in larval survival and success at metamorphosis. Larvae with low growth efficiency are successful under a much narrower range of culture conditions than larvae with high growth efficiency. The impact of low growth efficiency is primarily controlled by the ability of larvae to store lipid for metamorphosis. Culture conditions that provide increased dietary lipid counterweigh low growth efficiency. Changes in food quantity and quality had little effect on size at metamorphosis. On the other hand, larval life span and success rate at metamorphosis varied over a wide range depending upon the conditions of the simulation. Food quality and food availability both influence larval life span and, hence, larval survival. As ingestion rate decreases, larval life span increases and cohort survival declines. Increased lipid or decreased protein in the diet improves cohort survival. Changes in carbohydrate content are less influential. If cohort success is significantly affected by mortality during larval life rather than success at metamorphosis, the influence of food quality becomes more complex. The range of food compositions yielding high survival is restricted by a balance between improved success at metamorphosis obtained by increased lipid storage and the shortening of larval life span as a result of more rapid growth, a function of protein availability. These simulations illustrate the strength and utility of numerical models for evaluating and designing hatchery protocols for optimizing yield of C. gigas larvae.  相似文献   

110.
A procedure is described for the isolation of intact vitellogenin (c-VTG) from the carp, Cyprinus carpio. VTG was induced in juvenile females using oestradiol-17β and purified from the plasma using a combination of gel-filtration chromatography on Sepharose 6B and ion exchange chromatography on DEAE-cellulose. Purification procedures were conducted at low temperatures (below 9°C) in the presence of the proteolytic enzyme inhibitor aprotinin to prevent degradation. Intact c-VTG had an apparent molecular mass of 390,000 Daltons, but when extracted from plasma in the absence of aprotinin it underwent proteolysis into at least 2 protein fragments (apparent molecular masses of 230,000 and 96,000 Daltons), showing an instability of the native dimer. An amino acid analysis of c-VTG showed that its composition was almost identical to goldfish VTG, a species closely allied to the true carps and also similar to other oviparous vertebrate VTGs. Collectively, these data indicate that using these purification procedures VTG from carp, and probably other teleost species, can be isolated in an intact, highly purified form.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号