Socio-spatial organization reveals paternity and low kinship in the Honduran white bat (Ectophylla alba) in Costa Rica     

Edgar G. GUTIÉRREZ  Isabela VIVAS-TORO  Daniela CARMONA-RUÍZ  David VILLALOBOS-CHAVES  Bernal RODRÍGUEZ-HERRERA  Melina Del REAL-MONROY  Gloria LEÓN-AVILA  Jorge ORTEGA 《Integrative zoology》2021,16(5):646-658

Ectophylla alba is a tent-making bat that roosts in mixed-sex clusters comprising adults and offspring. Our goal was to determine the genetic identity of individuals belonging to different roosting groups. We tested the hypothesis of kin selection as a major force structuring group composition. We used 9 microsatellites designed for E. alba to determine the genetic identity and probability of parentage of individuals. We analyzed parentage and kinship using the software ML-Relate, GenAIEx, and Cervus. The obtained relationship probabilities (0.5) revealed a clear maternal relationship between female adults and offspring with allele compatibility, and at least 5 relationships between male adults and pups. We found a low degree of relatedness within roosting groups. Between roosting groups at different sites, the mean probability of a half-sibling relationship ranged from 0.214 to 0.244 and, for full-sibling relationship, from 0.383 to 0.553. Genetically, adult individuals were poorly related within clusters, and kinship as an evolutionary force could not explain group membership.  相似文献   

Microdialysis measurements of equine lamellar perfusion and energy metabolism in response to physical and pharmacological manipulations of blood flow          下载免费PDF全文

C. E. Medina‐Torres  C. Underwood  C. C. Pollitt  E. M. Castro‐Olivera  M. P. Hodson  D. W. Richardson  A. W. van Eps 《Equine veterinary journal》2016,48(6):756-764

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Predation performance and survival of susceptible and pyrethroid-resistant Eriopis connexa Germar (Coleoptera: Coccinellidae) to insecticides used in brassica crops     

Rogério Lira  Deividy Vicente do Nascimento  Paulo Roberto Ramos Barbosa  Alvin M. Simmons  Jorge Braz Torres 《Pest management science》2023,79(8):2704-2712

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Detection of Ecballium elaterium in hedgerow olive orchards using a low-cost uncrewed aerial vehicle and open-source algorithms     

Jorge Torres-Sánchez  Francisco Javier Mesas-Carrascosa  Fernando Pérez-Porras  Francisca López-Granados 《Pest management science》2023,79(2):645-654

BACKGROUND

Ecballium elaterium (common name: squirting cucumber) is an emerging weed problem in hedgerow or superintensive olive groves under no tillage. It colonizes the inter-row area infesting the natural or sown cover crops, and is considered a hard-to-control weed. Research in other woody crops has shown E. elaterium has a patchy distribution, which makes this weed susceptible to design a site-specific control strategy only addressed to E. elaterium patches. Therefore, the aim of this work was to develop a methodology based on the analysis of imagery acquired with an uncrewed aerial vehicle (UAV) to detect and map E. elaterium infestations in hedgerow olive orchards.

RESULTS

The study was conducted in two superintensive olive orchards, and the images were taken using a UAV equipped with an RGB sensor. Flights were conducted on two dates: in May, when there were various weeds infesting the orchard, and in September, when E. elaterium was the only infesting weed. UAV-orthomosaics in the first scenario were classified using random forest models, and the orthomosaics from September with E. elaterium as the only weed, were analyzed using an unsupervised algorithm. In both cases, the overall accuracies were over 0.85, and the producer's accuracies for E. elaterium ranged between 0.74 and 1.00.

CONCLUSION

These results allow the design of a site-specific and efficient herbicide control protocol which would represent a step forward in sustainable weed management. The development of these algorithms in free and open-source software fosters their application in small and medium farms. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.  相似文献   

Effect of sirolimus on insulin dynamics in horses     

Demia J. de Tonnerre  Carlos E. Medina Torres  Darko Stefanovski  Mary A. Robinson  Kate L. Kemp  François-René Bertin  Andrew W. van Eps 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):703-712

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Comparison of two postoperative complication grading systems after treatment of stifle and shoulder instability in 68 dogs     

Faolain M. Barrett BVM&S  Jason A. Bleedorn DVM MS  DACVS-SA  Kyle D. Hutcheson DVM  DACVS-SA  Bryan T. Torres DVM PhD  DACVS-SA  DACVSMR  Derek B. Fox DVM  PhD  DACVS 《Veterinary surgery : VS》2023,52(1):98-105

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Effects of Broomrape Parasitism on Sunflower Plants: Growth,Development, and Mineral Nutrition     

Esteban Alcántara  Marién Morales-García  Jorge Díaz-Sánchez 《Journal of plant nutrition》2013,36(7):1199-1206

Sunflower broomrape (Orobanche cumana Wallr.) is a parasitic plant that infects sunflower (Helianthus annuus L.) plants. In this work, sunflower plants were grown under greenhouse conditions in pots with the substrate infested or non-infested with broomrape seeds. At different numbers of days after sowing, plant height, internode lengths, number of leaves, head diameter, mineral composition of leaves, and potassium (K) concentration in stem were measured. The negative effects of broomrape parasitism were assessed from 57 d after sowing, when broomrape started to emerge. Parasitized plants exhibited lower shoot dry weight, height, and head diameter than control plants. The reduction in internode lengths was associated with a decrease in the gradient of K concentration from basal to apical stem. The mineral composition of leaves was also affected in parasitized plants. The concentrations of calcium (Ca), magnesium (Mg), manganese (Mn), and zinc (Zn) in leaves of parasitized plants were lower than those of the control plants, while there were few differences for K, phosphorus (P), iron (Fe), and copper (Cu). The effects of parasitism are discussed in relation to their competition for resources and to perturbations of the host physiology such as hormonal and water balance.  相似文献   

Study of some physicochemical and functional properties of quinoa (chenopodium quinoa willd) protein isolates     

Abugoch LE  Romero N  Tapia CA  Silva J  Rivera M 《Journal of agricultural and food chemistry》2008,56(12):4745-4750

The amino acid composition and the physicochemical and functional properties of quinoa protein isolates were evaluated. Protein isolates were prepared from quinoa seed by alkaline solubilization (at pH 9, called Q9, and at pH 11, called Q11) followed by isoelectric precipitation and spray drying. Q9 and Q11 had high levels of essential amino acids, with high levels of lysine. Both isolates showed similar patterns in native/SDS-PAGE and SEM. The pH effect on fluorescence measurements showed decreasing fluorescence intensity and a shift in the maximum of emission of both isolates. Q9 showed an endotherm with a denaturation temperature of 98.1 degrees C and a denaturation enthalpy of 12.7 J/g, while Q11 showed no endotherm. The protein solubility of Q11 was lower than that of Q9 at pH above 5.0 but similar at the pH range 3.0-4.0. The water holding capacity (WHC) was similar in both isolates and was not affected by pH. The water imbibing capacity (WIC) was double for Q11 (3.5 mL of water/g isolate). Analysis of DSC, fluorescence, and solubility data suggests that there is apparently denaturation due to pH. Some differences were found that could be attributed to the extreme pH treatments in protein isolates and the nature of quinoa proteins. Q9 and Q11 can be used as a valuable source of nutrition for infants and children. Q9 may be used as an ingredient in nutritive beverages, and Q11 may be used as an ingredient in sauces, sausages, and soups.  相似文献   

A Greener UV and Peroxide-Based Chemical Oxygen Demand Test     

Patricia Carbajal-Palacios  Patricia Balderas-Hernández  Gabriela Roa-Morales  Jorge G. Ibanez 《Water, air, and soil pollution》2017,228(8):313

Water quality assessment typically includes the determination of chemical oxygen demand (COD) by oxidation of organic matter with Cr(VI) in an acidic medium followed by digestion. Unfortunately, the required reagents are harmful and the reaction times are rather long. We investigated earlier the use of H₂O₂ as a more environmentally friendly oxidizing agent to replace the hazardous chromates. In the present study, we have furthered this possibility by incorporating the use of H₂O₂ in the presence of UV light. A protocol has been devised and tested with standards and real samples that replaces toxic Cr(VI), halves the amount of silver sulfate required, and greatly reduces the necessary reaction time, thus yielding a faster and more environmentally sound method.  相似文献   

Blood and haemoglobin meal as protein sources in diets for gilthead sea bream (Sparus aurata): effects on growth, nutritive efficiency and fillet sensory differences     

Silvia Martínez-Llorens  Ana Tomás Vidal  rés Vicente Moñino  Jose Gómez Ader  Marcial Pla Torres  & Miguel Jover Cerdá 《Aquaculture Research》2008,39(10):1028-1037

Two parallel experiments were conducted to evaluate the effect of partial substitution of fish meal by two different animal protein sources, blood meal (B) and haemoglobin meal (H) at 0, 50 and 100 g kg⁻¹ of level inclusion in diets for gilthead sea bream, considering a long feeding period for juveniles (Trial 1) and a short feeding period (Trial 2) for on-growing fish. In Trial 1, 33 g juveniles were fed for 242 days and the fish fed with 5% and 10% of haemoglobin dietary inclusion obtained less growth, although feed efficiency, protein efficiency ratio and muscle composition were similar in all diets. In Trial 2, 179 g initial weight fish were fed for 164 days and growth of fish fed H10 showed the lowest growth, although nutrient efficiency and muscle composition were not affected significantly. The results of these experiments demonstrated that blood meal can substitute fish meal (up to 10%) with no effect on performance, but may lead to sensory differences compared with fish fed diet 0, while the inclusion of 5% blood meal had no effect on growth or sensory characteristics. Fish fed 10% haemoglobin inclusion had the poorest growth values.  相似文献