Pathogenicity of plant and soil isolates of <Emphasis Type="Italic">Phytophthora parasitica</Emphasis> on tomato and pepper     

Amalia Boix-Ruiz  José Ignacio Marín-Guirao  Miguel de Cara-García  Francisco Camacho-Ferre  Julio César Tello-Marquina 《European journal of plant pathology / European Foundation for Plant Pathology》2017,148(3):607-615

Crown and root rot of tomato and sweet pepper can be caused by Phytophthora parasitica. In this work, 23 P. parasitica isolates from diseased pepper or tomato plants as well as 54 isolates from 23 monocrop tomato soils (from Spain and Chile) and one from a pepper soil were studied for their host–pathogen response. Results show significant host specificity for the isolates from tomato plants and tomato soils (63 of 64 isolates were unable to cause disease in pepper). None of the pepper plant/soil isolates showed pathogenicity on tomato, and only four of 14 reproduced their pathogenicity on pepper. Only one tomato isolate was pathogenic to both Solanaceae species. Two different inoculation protocols were evaluated (substrate irrigation and stem cutting). All isolates which expressed pathogenicity when stem inoculated also did it when root inoculated, but not vice-versa. Therefore, the recommended test protocol for tomato and pepper breeding programmes is that based on root inoculation by irrigation.  相似文献   

Behaviour of bentazon as influenced by water and tillage management in rice‐growing conditions          下载免费PDF全文

Antonio López‐Piñeiro  David Peña  Ángel Albarrán  Javier Sánchez‐Llerena  José Manuel Rato‐Nunes  María Ángeles Rozas 《Pest management science》2017,73(6):1067-1075

Bentazon is a widely used herbicide in rice agroecosystems that has commonly been found in water resources. To assess how tillage and water regimes affect sorption/desorption, dissipation and leaching of bentazon in Mediterranean rice‐growing conditions, field experiments were carried out using tillage and flooding (TF), tillage and sprinkler irrigation (TS), no‐tillage and sprinkler irrigation (NTS) and long‐term no‐tillage and sprinkler irrigation (NTS7). After 3 years, the K_d values in TS were 2.3, 1.6 and 1.7 times lower than the values in NTS7, NTS and TF respectively. Greater sorption of bentazon was related to higher contents in total organic carbon and, although to a lesser extent, in humic acids and dissolved organic carbon. The persistence of bentazon was significantly greater under anaerobic (half‐life DT₅₀ = 94.1–135 days) than under aerobic (DT₅₀ = 42.4–91.3 days) incubation conditions for all management regimes. Leaching losses of bentazon were reduced from 78 and 74% in TS and TF to 61 and 62% in NTS7 and NTS respectively. The mid‐ and long‐term implementation of sprinkler irrigation in combination with no‐tillage could be considered a management system that is effective at reducing water contamination by bentazon in Mediterranean rice‐growing agroecosystems. © 2017 Society of Chemical Industry  相似文献   

Pulse pressure variation as a guide for volume expansion in dogs undergoing orthopedic surgery     

Denise T. Fantoni  Keila K. Ida  André M. Gimenes  Matheus M. Mantovani  Jacqueline R. Castro  Geni C.F. Patrício  Aline M. Ambrósio  Denise A. Otsuki 《Veterinary anaesthesia and analgesia》2017,44(4):710-718

Objective

To investigate whether pulse pressure variation (PPV) can predict fluid responsiveness in healthy dogs during clinical surgery.

Study design

Prospective clinical study.

Animals

Thirty-three isoflurane-anesthetized dogs with arterial hypotension during orthopedic surgery.

Methods

Fluid challenge with lactated Ringer's solution (15 mL kg^?1 in 15 minutes) was administered in mechanically ventilated dogs (tidal volume 10 mL kg^?1) with hypotension [mean arterial pressure (MAP) < 65 mmHg]. The volume expansion was considered effective if cardiac output (CO; transesophageal Doppler) increased by ≥ 15%. Cardiopulmonary data were analyzed using two-way ANOVA, receiver operating characteristics (ROC) curves and Spearman coefficient; p < 0.05 was considered significant.

Results

Effective volume expansion, mean ± standard deviation 42 ± 4% increase in CO (p < 0.0001) was observed in 76% of the dogs, resulting in a decrease in PPV (p < 0.0001) and increase in MAP (p < 0.0001), central venous pressure (CVP; p = 0.02) and ejection fraction (p < 0.0001) compared with before the fluid challenge. None of these changes occurred when volume expansion resulted in a nonsignificant CO increase of 4 ± 5%. No significant differences were observed in blood gas analysis between responsive and nonresponsive dogs. The increase in CO was correlated with the decrease in PPV (r = ?0.65; p < 0.0001) but absolute values of CO and PPV were not correlated. The PPV performance (ROC curve area: 0.89 ± 0.06, p = 0.0011) was better than that of CVP (ROC curve area: 0.54 ± 0.12) and MAP (ROC curve area: 0.59 ± 0.13) to predict fluid responsiveness. The best cut-off for PPV to distinguish responders and nonresponders was 15% (50% sensitivity and 96% specificity).

Conclusions and clinical relevance

In mechanically ventilated, healthy, isoflurane-anesthetized dogs, PPV predicted fluid responsiveness to volume expansion, and MAP and CVP did not show such applicability.  相似文献   

Cardiopulmonary effects of reverse Trendelenburg position at 5° and 10° in sevoflurane-anesthetized steers     

Marcelo A. Araújo  Maurício Deschk  Juliana T. Wagatsuma  Beatriz P. Floriano  Carlos E. Siqueira  Valéria NLS. Oliva  Paulo SP. Santos 《Veterinary anaesthesia and analgesia》2017,44(4):854-864

Objective

To assess the cardiopulmonary effects caused by reverse Trendelenburg position (RTP) at 5° and 10° in sevoflurane-anesthetized yearling steers.

Study design

Prospective, experimental study.

Animals

Eight Holstein steers aged (mean ± standard deviation) 12 ± 2 months and weighing 145 ± 26 kg.

Methods

In the first phase of the study, the individual minimum alveolar concentration (MAC) of sevoflurane was determined using electrical stimulation. In the second phase, the effects of RTP were assessed. The animals were anesthetized on three separate events separated by ≥7 days in an incomplete crossover design: control treatment using a table without tilt (RTP0); treatment with the table at 5° RTP (RTP5) and table tilted 10° RTP (RTP10). Subjects were physically restrained in dorsal recumbency on the table, which was already tilted according to each treatment. Anesthesia was induced with sevoflurane at 8% in 5 L minute^–1 oxygen via face mask followed by maintenance with sevoflurane at 1.3 MAC and spontaneous breathing. Cardiopulmonary variables were obtained immediately after instrumentation (T₀) and then after 30, 60, 120 and 180 minutes (T₃₀, T₆₀, T₁₂₀ and T₁₈₀, respectively).

Results

The mean sevoflurane MAC for the eight steers was 2.12 ± 0.31%. Cardiac output was lower at all time points and the systemic vascular resistance index was higher at T₁₂₀ and T₁₈₀ in RTP10 compared with RTP0. Oxygen consumption was lower at T₀ and at T₁₈₀ in RTP10 compared with RTP0 and at all time points except T₃₀ compared with RTP5. Oxygen extraction was lower at T₀ in RTP10 compared with RTP0 and RTP5, and at T₆₀ and T₁₈₀ compared with RTP5.

Conclusions and clinical relevance

RTP 5° and 10° did not improve ventilatory and oxygenation variables in sevoflurane-anesthetized steers when compared with no tilt, however the cardiovascular variables were adversely affected in RTP10.  相似文献   

Hyaluronidase administered with xylazine–tiletamine–zolazepam into adipose tissue shortens recovery from anesthesia in pigs     

Shayne P. Bisetto  Adriano B. Carregaro  André E.S. Nicolai  Thais F. Bressan  William P. Leal  Nathalia V. Xavier  Diego F. Leal  André F.C. Andrade 《Veterinary anaesthesia and analgesia》2017,44(3):594-599

Objective

To evaluate the effect of hyaluronidase on uptake, duration and speed of elimination of xylazine–tiletamine–zolazepam administered in the subcutaneous fat over the dorsal lumbar region of swine.

Study design

Blinded, randomized, crossover study.

Animals

Six healthy Landrace/Large White pigs weighing 132 ± 24 kg (mean ± standard deviation).

Methods

Animals were administered xylazine (1 mg kg^?1) and tiletamine–zolazepam (8 mg kg^?1) (control treatment, CON), or xylazine–tiletamine–zolazepam at the same doses with hyaluronidase (400 IU) (treatment HYA). The treatments were administered into the dorsal lumbar adipose tissue, 2.5–3.0 cm laterally from the spinous process of the second lumbar vertebra. The latency, anesthesia and recovery periods were measured. Heart rate, noninvasive systolic, diastolic, and mean arterial pressures, respiratory rate, hemoglobin oxygen saturation and rectal temperature were recorded every 10 minutes for up to 50 minutes.

Results

One animal in CON and one animal in HYA were responsive to stimulation and did not allow safe handling. No significant difference was found between treatments for latency (CON 11.3 ± 5.9 minutes, HYA 7.4 ± 5.1 minutes) and anesthesia (CON 53 ± 53 minutes, HYA 49 ± 38 minutes) periods. Recovery period was shorter in HYA (9 ± 6 minutes) than in CON (32 ± 16 minutes) (p < 0.05). Physiological variables were not significantly changed over time and were within accepted normal clinical limits for the species in both treatments.

Conclusion and clinical relevance

Hyaluronidase (400 IU) administered into adipose tissue in pigs did not reduce the latency and duration of dissociative anesthesia, but was associated with faster recovery.  相似文献   

The effect of user experience and inflation technique on endotracheal tube cuff pressure using a feline airway simulator     

Donna M. White  José I. Redondo  Alastair R. Mair  Fernando Martinez-Taboada 《Veterinary anaesthesia and analgesia》2017,44(5):1076-1084

Objective

The effect of user experience and inflation technique on endotracheal tube cuff pressure using a feline airway simulator.

Study design

Prospective, experimental clinical study.

Methods

Participants included veterinary students at the beginning (group S1) and end (group S2) of their 2-week anaesthesia rotation and veterinary anaesthetists (group A). The feline airway simulator was designed to simulate an average size feline trachea, intubated with a 4.5 mm low-pressure, high-volume cuffed endotracheal tube, connected to a Bain breathing system with oxygen flow of 2 L minute^?1. Participants inflated the on-endotracheal tube cuff by pilot balloon palpation and by instilling the minimum occlusive volume (MOV) required for loss of airway leaks during manual ventilation. Intracuff pressures were measured by manometers obscured to participants and ideally were 20–30 cm H₂O. Student t, Fisher exact, and Chi-squared tests were used where appropriate to analyse data (p < 0.05).

Results

Participants were 12 students and eight anaesthetists. Measured intracuff pressures for palpation and MOV, respectively, were 19 ± 12 and 29 ± 19 cm H₂O for group S1, 10 ± 5 and 20 ± 11 cm H₂O for group S2 and 13 ± 6 and 29 ± 18 cm H₂O for group A. All groups performed poorly at achieving intracuff pressures within the ideal range. There was no significant difference in intracuff pressures between techniques. Students administered lower (p = 0.02) intracuff pressures using palpation after their training.

Conclusions and clinical relevance

When using palpation and MOV for cuff inflation operators rarely achieved optimal intracuff pressures. Experience had no effect on this skill and, as such, a cuff manometer is recommended.  相似文献   

Biofilms of Candida spp. from the ocular conjunctiva of horses with reduced azole susceptibility: a complicating factor for the treatment of keratomycosis?          下载免费PDF全文

Raimunda Sâmia Nogueira Brilhante  Paula Vago Bittencourt  Débora de Souza Collares Castelo‐Branco  Glaucia Morgana de Melo Guedes  Jonathas Sales de Oliveira  Lucas Pereira Alencar  Rossana de Aguiar Cordeiro  Mariana Pinheiro  Evilázio Fernandes Nogueira‐Filho  Waldemiro de Aquino Pereira‐Neto  José Júlio Costa Sidrim  Marcos Fábio Gadelha Rocha 《Veterinary ophthalmology》2017,20(6):539-546

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Retrospective evaluation of 155 adult equids and 21 foals with tetanus in Western,Northern, and Central Europe (2000–2014). Part 1: Description of history and clinical evolution          下载免费PDF全文

Gaby van Galen DVM  PhD  DECEIM  DECVECC  Claude Saegerman DVM  PhD  DECVPH  Joke Rijckaert DVM  Helene Amory DVM  PhD  DECEIM  Lara Armengou DVM  PhD  DECEIM  Barbora Bezdekova DVM  PhD  DECEIM  Inge Durie DVM  DECEIM  Rikke Findshøj Delany DVM  Nathalie Fouché DVM  DVM  Laura Haley MVB  Michael Hewetson BVSc  DECEIM  Rene van den Hoven DVM  PhD  DECEIM  Anna Kendall DVM  DECEIM  Fernando Malalana DVM  DECEIM  MRCVS  Jessika Muller Cavalleri DVM  DECEIM  PhD  Tresemiek Picavet DVM  DECEIM  PhD  Katja Roscher DVM  DECEIM  Denis Verwilghen DVM  PhD  DECVS  Meret Wehrli Eser DVM  DECEIM  Cornélie Westermann DVM  PhD  DECEIM  Tim Mair BVSc  PhD  DECEIM 《Journal of Veterinary Emergency and Critical Care》2017,27(6):684-696

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The influence of Ringer's lactate or HES 130/0.4 administration on the integrity of the small intestinal mucosa in a pig hemorrhagic shock model under general anesthesia          下载免费PDF全文

Ana Liza Ortiz DVM  PhD  Helena Vala DVM  PhD  Carlos Venâncio DVM  PhD  João Mesquita DVM  PhD  Aura Silva DVM  PhD  Jose Manuel Gonzalo‐Orden DVM  PhD  David Ferreira DVM  PhD 《Journal of Veterinary Emergency and Critical Care》2017,27(1):96-107

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Effects of Vermicompost and Vermiwash on Plant,Phenolic Content,and Anti-oxidant Activity of Mexican Pepperleaf (Piper auritum Kunth) Cultivated in Phosphate Rock Potting Media     

María Celina Luján-Hidalgo  Deisy Ermeth Gómez-Hernández  Juan José Villalobos-Maldonado  Miguel Abud-Archila  Joaquín Adolfo Montes-Molina  Samuel Enciso-Saenz 《Compost science & utilization》2017,25(2):95-101

The aim of this investigation was to determine the effect of vermicompost, vermiwash, and phosphate rock on plant, total phenols, flavonoids, and anti-oxidant activity in Piper auritum Kunth leaves. P. auritum plants were obtained from cuttings and were planted according to the Box-Behnken experimental design with three repetitions at the central point. The factors and levels were vermicompost (10, 20, and 30 g plant^?1), vermiwash (5, 10, and 15 mL plant^?1), and phosphate rock (1, 2, and 3 g plant^?1). Plant growth parameters (plant height, stem diameter, leaves number) and chlorophyll content were measured 1 month after treatment applications. Total phenols, total flavonoids, and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity was measured after 4 months. Vermicompost, vermiwash, and phosphate rock had no statistically significant effect on plant growth. Plant height, stem diameter, leaves number, chlorophyll, innermost number, fresh weight stem, fresh weight leaves, fresh weight root, dry weight stem, dry weight leaves, and dry weight root were not different among treatments. Total phenolic compounds were statistically affected for both vermiwash and phosphoric rock (p < 0.05) and the anti-oxidant activity decreased by vermicompost addition. The application of 15 mL plant^?1 vermiwash, 1 g phosphate rock, and 20 g vermicompost plant^?1 increased the total phenol content.  相似文献