Adherence of Actinobacillus pleuropneumoniae serotype 1 to swine buccal epithelial cells involves fibronectin          下载免费PDF全文

Roberto Hamer-Barrera  Delfino Godínez  V. Idalia Enríquez  Sergio Vaca-Pacheco  Rodrigo Martínez-Zú?iga  Patricia Talamás-Rohana  Francisco Suárez-Güemez    Mireya de la Garza 《Canadian journal of veterinary research》2004,68(1):33-41

The swine pathogen Actinobacillus pleuropneumoniae serotype 1 was investigated for its ability to adhere to swine, rat, and human buccal epithelial cells (BEC). The highest number of bacteria adhered was to swine BEC. This binding ability was affected by heating, extreme pH, treatment with sodium dodecyl sulfate, ethylenediamine tetraacetate, or periodate, and proteolysis, suggesting that cell-surface glycoproteins participate in adherence and that adherence is based mostly on ionic interactions. Mannose and swine fibronectin may play a direct role in this interaction. Convalescent-phase serum from naturally infected pigs inhibited the adhesion. There was a correlation between bacterial pathogenicity as well as host specificity and the capacity for adherence to swine BEC. Adhesion to swine BEC provides a convenient method to study in vitro the adherence of A. pleuropneumoniae and other pathogens of the pig respiratory tract.  相似文献   

Identification and Characterisation of Bacteria Causing Soft-rot in Agave tequilana     

I. Jiménez-Hidalgo  G. Virgen-Calleros  O. Martínez-de la Vega  G. Vandemark  V. Olalde-Portugal 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(3):317-331

Agave tequilana is the raw material for the production of the alcoholic beverage tequila. A bacterial disease has affected the A. tequilana crop in recent years. Previous reports based on colony and cell morphology, Gram stain and potato rot indicated that Erwinia sp. is the main pathogen. We isolated a several bacterial isolates capable of producing soft-rot symptoms in greenhouse pathogenicity assays. An extensive characterisation involving pathogenicity tests, fatty acid profile, metabolic and physiological properties, ribosomal DNA sequence and intergenic transcribed spacer amplification (ITS-PCR) and restriction banding pattern (ITS-RFLP) was made of each isolate. Three different species: Erwinia cacticida, Pantoea agglomerans and Pseudomonas sp. were identified. Fatty acid and metabolic profiles gave low similarity values of identification but 16S rDNA sequence, ITS-PCR and ITS-RFLP confirmed the identification of E. cacticida. In the phylogenetic tree, E. cacticida from blue agave was grouped neither with E. cacticida type strains nor with Erwinia carotovora. This is the first report that associates E. cacticida with A. tequilana soft-rot symptoms.  相似文献   

Evaluation of Olive Cultivars for Resistance to Verticillium dahliae     

F.J. López-Escudero  C. del Río  J.M. Caballero  M.A. Blanco-López 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(1):79-85

Resistance of 23 important olive cultivars to Verticillium dahliae has been evaluated in four experiments under controlled conditions. Nine-month-old nursery olive plants were inoculated with a cotton non-defoliating (ND) (V4) or a cotton defoliating (D) (V117) isolate of V. dahliae. Resistance was evaluated by assessing symptom severity using a 0–4 rating scale and estimating the area under disease progress curves. The percentage of plants killed and of those which recovered from the disease were used as additional parameters for including a particular cultivar into a defined category. Most of the evaluated cultivars were susceptible, although at different levels, to both isolates of V. dahliae. All cultivars were more susceptible to the D pathotype than to the ND one. A group of 11 cultivars, including several important Spanish cultivars, were susceptible or extremely susceptible to both pathotypes of V. dahliae. A second group showed differences of resistance depending on the pathotype used. They were susceptible or extremely susceptible to the D pathotype but resistant or moderately susceptible to the ND one. Finally, 'Frantoio', 'Oblonga' and 'Empeltre' were moderately susceptible to the D isolate of V. dahliae and resistant to the ND one. The resistance of 'Empeltre' was evident by the plant ability to recover from infection with either isolates. 'Empeltre' is considered to be a valuable cultivar for inclusion in breeding programmes for resistance to Verticillium wilt.  相似文献   

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Eva Patricia López‐Damián  José Alfredo Jiménez‐Medina  Miguel Angel Lammoglia  Jaime Arturo Pimentel  Lourdes Teresa Agredano‐Moreno  Chris Wood  Carlos Salvador Galina  Tatiana Fiordelisio 《Anatomia, histologia, embryologia》2018,47(5):i-i

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Fracture of the medial intercondylar eminence of the tibia in horses treated by arthroscopic fragment removal (21 horses)          下载免费PDF全文

L. M. Rubio‐Martínez  W. R. Redding  B. Bladon  H. Wilderjans  R. J. Payne  C. Tessier  O. Geffroy  R. Parker  C. Bell  F. A. Collingwood 《Equine veterinary journal》2018,50(1):60-64

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Surgical repair of propagating condylar fractures of the third metacarpal/metatarsal bones with cortical screws placed in lag fashion in 26 racehorses (2007–2015)          下载免费PDF全文

N. Moulin  I. François  N. Coté  C. Alford  O. Cleary  M. R. Desjardins 《Equine veterinary journal》2018,50(5):629-635

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Effect of ionization,bedding, and feeding on air quality in a horse stable          下载免费PDF全文

Esther Willemijn Siegers  Milou Anthonisse  Frank J. C. M. van Eerdenburg  Jan van den Broek  Inge M. Wouters  Cornélie Martine Westermann 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):1234-1240

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Serologic and fecal markers to predict response to induction therapy in dogs with idiopathic inflammatory bowel disease          下载免费PDF全文

Cristiane C. Otoni  Romy M. Heilmann  Mercedes García‐Sancho  Angel Sainz  Mark R. Ackermann  Jan S. Suchodolski  Jörg M. Steiner  Albert E. Jergens 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):999-1008

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Congenital duodenocolic fistula in a dog          下载免费PDF全文

A. Lecoindre  D. Saade  P. Barthez  J. L. Cadoré  P. Lecoindre 《The Journal of small animal practice》2018,59(5):311-314

A one‐year‐old female cocker spaniel presented with a 6‐month history of persistent diarrhoea. Abdominal ultrasonographic examination revealed mild diffuse thickening of the intestinal wall coupled with mesenteric lymphadenopathy. A connection between the duodenum and the colon was observed during an endoscopic procedure and confirmed by computed tomography. Surgical resection of the communication allowed remission of the diarrhoea. Histology showed a normal duodenal epithelium and muscular layer. A duodenocolic fistula is an abnormal connection within the digestive tract, which in humans is usually considered a complication of a local pathological condition. Due to the absence of a predisposing cause and, in view of the dog's age and histological results, a congenital origin was suspected.  相似文献   

Use of two doses of cloprostenol in different intervals for estrus synchronization in hair sheep under tropical conditions     

de Carvalho Menezes de Almeida  Sheylla Foligno  Souza-Fabjan  Joanna Maria Gonçalves  Balaro  Mario Felipe Alvarez  Bragança  Gláucia Mota  Pinto  Pedro Henrique Nicolau  de Almeida  José Gabriel  Moura  Ana Beatriz Bossois  da Fonseca  Jeferson Ferreira  Brandão  Felipe Zandonadi 《Tropical animal health and production》2018,50(2):427-432

Tropical Animal Health and Production - This study evaluated the effect of two doses of prostaglandin at different intervals on reproductive parameters of crossbred ewes. In Experiment 1, 30 ewes...  相似文献