HlMyb3, a putative regulatory factor in hop (Humulus lupulus L.), shows diverse biological effects in heterologous transgenotes     

Matousek J  Kocábek T  Patzak J  Skopek J  Maloukh L  Heyerick A  Fussy Z  Roldán-Ruiz I  Keukeleire DD 《Journal of agricultural and food chemistry》2007,55(19):7767-7776

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Measurement uncertainty associated with sample processing of oranges and tomatoes for pesticide residue analysis     

Fussell RJ  Hetmanski MT  Macarthur R  Findlay D  Smith F  Ambrus A  Brodesser PJ 《Journal of agricultural and food chemistry》2007,55(4):1062-1070

The homogeneity of analytical samples and the stability of pesticides during the sample processing of oranges and tomatoes were evaluated. The mean concentrations of 14C-labeled chlorpyrifos in analytical portions (subsamples) after processing show that homogeneity is dependent on sample type as well as the processing procedure. The homogeneity of analytical samples of tomatoes processed cryogenically was much better than those processed at ambient temperature. For tomatoes, the minimum analytical portion masses required for between-analytical portion variation of < 0.3 Ho were 110 and 5 g for processing at ambient and cryogenic temperatures, respectively. Results for orange showed that analytical portion sizes of 5 g provided sufficient homogeneity from both sample processing procedures. Assessments of pesticide stability demonstrated that most were relatively stable during processing at either ambient or cryogenic temperatures. However, some pesticides, including dichlofluanid, chlorothalonil, tolylfluanid, and dicloran, appeared to suffer much greater losses (>20%) during processing at ambient temperature. For these analytes, loss is interpreted as chemical degradation.  相似文献   

Food for thought: lower-than-expected crop yield stimulation with rising CO2 concentrations   总被引：2，自引：0，他引：2  

Long SP  Ainsworth EA  Leakey AD  Nösberger J  Ort DR 《Science (New York, N.Y.)》2006,312(5782):1918-1921

Model projections suggest that although increased temperature and decreased soil moisture will act to reduce global crop yields by 2050, the direct fertilization effect of rising carbon dioxide concentration ([CO2]) will offset these losses. The CO2 fertilization factors used in models to project future yields were derived from enclosure studies conducted approximately 20 years ago. Free-air concentration enrichment (FACE) technology has now facilitated large-scale trials of the major grain crops at elevated [CO2] under fully open-air field conditions. In those trials, elevated [CO2] enhanced yield by approximately 50% less than in enclosure studies. This casts serious doubt on projections that rising [CO2] will fully offset losses due to climate change.  相似文献   

Sequence analysis of a "true" chalcone synthase (chs_H1) oligofamily from hop (Humulus lupulus L.) and PAP1 activation of chs_H1 in heterologous systems     

Matousek J  Vrba L  Skopek J  Orctova L  Pesina K  Heyerick A  Baulcombe D  De Keukeleire D 《Journal of agricultural and food chemistry》2006,54(20):7606-7615

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Predicting depth translocation of base cations after forest liming: results from long-term experiments     

Anja Guckland  Bernd Ahrends  Uwe Paar  Inge Dammann  Jan Evers  Karl Josef Meiwes  Egbert Sch?nfelder  Thomas Ullrich  Michael Mindrup  Nils K?nig  Johannes Eichhorn 《European Journal of Forest Research》2012,131(6):1869-1887

Forest liming is a common measure to counteract soil acidification. In forest practice, lime is applied to the forest floor where it changes the chemical properties. However, little is known about the depth impact of liming and the depth translocation of lime components. To investigate the long-term impact of forest liming, several study plots have been established in the 1980s in Germany in stands with different site conditions. We analysed soil chemical data obtained during the last 28?years from 45 of the study plots. We examined the depth impact of liming and predicted the main factors responsible for the increase in Calcium (Ca) and Magnesium (Mg) stocks after liming in the mineral soil using multiple linear regression analyses (MLR). Stocks of Ca and Mg as well as base saturation (BS) showed a strong depth gradient with significant differences between limed and control plots down to 40?cm of the mineral soil. About 65–70?% of applied Ca and Mg were recovered in the forest floor and the upper 40?cm of the mineral soil. BS in 0–40?cm increased by a mean of 11?%. MLR models could explain 48–74?% of the variation in mean changes of Ca and Mg in 0–10, 10–20 and 20–40?cm soil depth when soil and climate variables, amount of applied lime and years after liming are included in the model. After testing the model robustness with a cross-validating procedure, we concluded that these models might be applied to many regions in Central Europe with comparable soil and climate conditions and thus, have widespread application.  相似文献   

Detection and molecular characterization of Suid herpesvirus type 1 in Austrian wild boar and hunting dogs     

Steinrigl A  Revilla-Fernández S  Kolodziejek J  Wodak E  Bagó Z  Nowotny N  Schmoll F  Köfer J 《Veterinary microbiology》2012,157(3-4):276-284

Aujeszky's disease (AD), caused by Suid herpesvirus type 1 (SuHV-1), is an economically important disease in domestic swine. Thus, rigorous control programmes have been implemented and consecutively AD in domestic swine was successfully eradicated in many countries, including Austria. However, SuHV-1 continues to thrive in wild boar populations, as indicated by high seroprevalences in a number of European countries and by occasional cases of AD in hunting dogs. For the first time, SuHV-1 was detected in Austrian wild boar and a molecular characterization of SuHV-1 isolated from wild boar and hunting dogs was performed. Results of preliminary serological analyses suggest a regional SuHV-1 seroprevalence of over 30% in free-living and almost 70% in fenced wild boar from Eastern Austria. Molecular typing of Austrian SuHV-1 isolates of wild boar origin revealed the presence of two genetically distinct variants of SuHV-1, both capable of infecting dogs that have been exposed to infected wild boar during hunting.  相似文献   

Production of giant mouse oocyte nucleoli and assessment of their protein content     

Fulka H  Martinkova S  Kyogoku H  Langerova A  Fulka J 《The Journal of reproduction and development》2012,58(3):371-376

Compared with advanced developmental stage embryos and somatic cells, fully grown mammalian oocytes contain specific nucleolus-like structures (NPB - nucleolus precursor bodies). It is commonly accepted that they serve as a store of material(s) from which typical nucleoli are gradually formed. Whilst nucleoli from somatic cells can be collected relatively easily for further biochemical analyses, a sufficient number of oocyte nucleoli is very difficult to obtain. We have found that isolated oocytes nucleoli fuse very efficiently when contact is established between them. Thus, well visible giant nucleoli can be obtained, relatively easily handled and then used for further biochemical analyses. With the use of colloidal gold staining, we estimated that a single fully grown mouse oocyte nucleolus contains approximately 1.6 ng of protein. We do believe that this approach will accelerate further research aiming at analyzing the composition of oocyte nucleoli in more detail.  相似文献   

Incidence of seizures associated with iopamidol or iomeprol myelography in dogs with intervertebral disk disease: 161 cases (2000–2002)     

Leona Lexmaulova MVDr  Josef Zatloukal MVDr  PhD  Pavel Proks MVDr  Milan Dvorak MVDr  PhD  Robert Srnec MVDr  Petr Rauser MVDr  PhD  Helga Kecova MVDr  PhD  Alois Necas MVDr  PhD  MBA 《Journal of Veterinary Emergency and Critical Care》2009,19(6):611-616

Objective – To compare the incidence of seizures in dogs with intervertebral disk disease after iopamidol or iomeprol myelography, and to assess whether the incidence of seizures differed between the 2 agents when severity of neurological deficits, location of cord compression, duration of anesthesia, site of myelogram, volume of contrast, and concentration of contrast were evaluated. Design – Retrospective study. Setting – Veterinary teaching hospital. Animals – One hundred and sixty‐one client‐owned dogs with intervertebral disk disease. Interventions – Subarachnoid injection of contrast medium. Measurements and Main Results – One hundred and sixty‐one dogs with intervertebral disk disease were subjected to myelography using iopamidol (n=74) or iomeprol (n=87). Cranial myelography was performed in 31 dogs, caudal myelography in 125 and both cranial and caudal myelography in 5. Seizures occurred in 23 of 161 (14%) dogs. There was no significant difference overall between iopamidol and iomeprol myelography. However, in dogs with thoracolumbar disk extrusion and paraplegia, seizures occurred more frequently after caudal myelography using iopamidol compared with iomeprol. Conclusions – Both iomeprol and iopamidol are suitable for myelography in dogs. Iomeprol is recommended for caudal myelography in paraplegic dogs with thoracolumbar disk extrusion due to the higher incidence of seizures in this group when iopamidol was used.  相似文献   

Organic farming in isolated landscapes does not benefit flower-visiting insects and pollination     

Claire Brittain  Riccardo Bommarco  Josef Settele 《Biological conservation》2010,143(8):1860-167

Organic farming has often been found to provide benefits for biodiversity, but the benefits can depend on the species considered and characteristics of the surrounding landscape. In an intensively farmed area of Northeast Italy we investigated whether isolated organic farms, in a conventionally farmed landscape, provided local benefits for insect pollinators and pollination services. We quantified the relative effects of local management (i.e. the farm system), landscape management (proportion of surrounding uncultivated land) and interactions between them. We compared six organic and six conventional vine fields. The proportion of surrounding uncultivated land was calculated for each site at radii of 200, 500, 1000 and 2000 m. The organic fields did not differ from the conventional in their floral resources or proportion of surrounding uncultivated land. Data were collected on pollinator abundance and species richness, visitation rates to, and pollination of experimental potted plants. None of these factors were significantly affected by the farming system. The abundance of visits to the potted plants in the conventional fields tended to be negatively affected by the proportion of surrounding uncultivated land. The proportion fruit set, weight of seeds per plant and seed weight in conventional and organic fields were all negatively affected by the proportion of surrounding uncultivated land. In vine fields the impact of the surrounding landscape was stronger than the local management. Enhancement of biodiversity through organic farming should not be assumed to be ubiquitous, as potential benefits may be offset by the crop type, organic-management practices and the specific habitat requirements in the surrounding landscape.  相似文献   

An AM fungus and a PGPR intensify the adverse effects of salinity on the stability of rhizosphere soil aggregates of Lactuca sativa     

Josef Kohler  Antonio Roldán 《Soil biology & biochemistry》2010,42(3):429-434

A mesocosm experiment was conducted to examine the effect of an arbuscular mycorrhizal (AM) fungus (Glomus mosseae (Nicol & Gerd.) Gerd. & Trappe) and a plant growth-promoting rhizobacterium (PGPR) (Pseudomonas mendocina Palleroni), alone or in combination, on the structural stability of the rhizosphere soil of Lactuca sativa L. grown under two levels of salinity. The plants inoculated with P. mendocina had significantly greater shoot biomass than the control plants at both salinity levels, whereas the mycorrhizal inoculation was only effective in increasing shoot biomass at the moderate salinity level. The aggregate stability of soils inoculated with the PGPR and/or G. mosseae significantly decreased with increasing saline stress (about 29% lower than those of soils under non-saline conditions). Only the inoculated soils showed higher concentrations of sodium (Na) under severe saline stress. The severe salinity stress decreased the glomalin-related soil protein (GRSP) concentration, but the highest values of GRSP were recorded in the inoculated soils. Our findings suggest that the use of AM fungi and/or a PGPR for alleviating salinity stress in lettuce plants could be limited by their detrimental effect on soil structural stability.  相似文献