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An agrarian sensorial system based on temperature, moisture, and all solid-state ion-selective potentiometric sensors was developed with the objective of monitoring the behavior of H+ and Ca2+ ions in soil and in real conditions, contributing with a new tool that tries to complement the current precision agriculture technology. The evaluation of the sensorial system to pH monitoring presented a good correlation between the results obtained by the system and the standard methodology, allowing us to notice the soil buffer capacity at different soil depths. With regard to calcium, the sensor system also presented an agreement between its results and those obtained by flame atomic absorption spectrometry, using a calibration model based on multiple linear regressions that allows the correct determination of Ca2+ concentrations in soil depths where the relative moisture is different. In this way, using well-known potentiometric sensors in a complex, discontinued, and heterogeneous matrix, such as soil, the sensorial system proved to be a useful task for agrochemical field applications.  相似文献   
495.
Molecular diagnostic methods using the polymerase chain reaction (PCR) are the gold standard in Helicobacter diagnostics. Most rely on the amplification of parts of the 16S rRNA gene sequence. Therefore, the validity and accuracy of results depends heavily on the PCR design and the time of its publication because new sequences are continually being submitted to databases. Here we report the presence of helicobacter in commercially bred mice supposedly free of this infection. Furthermore, three out of six different commercial laboratories performing helicobacter testing on the same spiked faecal samples failed to detect and identify H. hepaticus. We designed a simple generic PCR assay that amplifies a 261bp amplicon spanning two of the seven variable regions in the 16S rRNA of helicobacter. Using this assay together with an established generic assay designed by Bohr [Bohr, U.R., Primus, A., Zagoura, A., Glasbrenner, B., Wex, T., Malfertheiner, P., 2002. A group-specific PCR assay for the detection of Helicobacteraceae in human gut. Helicobacter 7, 378-383] and then cloning and sequencing their products, we detected the H. hepaticus used in the study that three commercial laboratories failed to detect. We think these assays together could detect all the currently known species of helicobacter and hopefully the new ones as well. In addition, we have been able to identify different species of helicobacter and their relative proportions infecting a single animal. This information has also shown that some helicobacters may have a much broader host range than originally reported.  相似文献   
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小麦品种抗吸浆虫鉴定   总被引:10,自引:2,他引:10  
1991~1994年利用一套较完整的田间抗性鉴定方法,对443个生产品种、后备品种及抗源进行了抗吸浆虫鉴定。结果表明,参试品种(系)对吸浆虫的抗性差异显著。高抗、中抗和低抗的品种(系)分别占参试材料的13.1%、12.9%和28.1%,其中冀麦23、24和25号、遗4179、4154、4188、郑太育一号等18个品种(系)对吸浆虫连续几年表现出稳定的抗性。鉴定结果为生产和育种部门利用抗虫品种提供了科学依据。  相似文献   
498.
BACKGROUND: The spread of the western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), resulted in the worldwide destabilization of established integrated pest management programs for many crops. Efforts to control the pest and the thrips‐vectored tospoviruses with calendar applications of broad‐spectrum insecticides have been unsuccessful. The result has been a classic ‘3‐R’ situation: resistance to numerous insecticides; resurgence of the western flower thrips populations as a result of natural predators and native competitor thrips being eliminated; replacement by various other pests. This paper reports on integrated pest management programs for fruiting vegetables that are effective, economical, ecologically sound and sustainable. RESULTS: The components include the following: define pest status (economic thresholds); increase biotic resistance (natural enemies and competition); integrate preventive and therapeutic tactics (scouting, ultraviolet‐reflective technologies, biological control, compatible insecticides, companion plants and fertility); vertically integrate the programs with other pests; continually communicate latest science‐based management tactics with end‐users. CONCLUSION: These programs have been widely implemented in Florida and have significantly improved the management of western flower thrips and thrips‐transmitted viruses. Copyright © 2012 Society of Chemical Industry  相似文献   
499.
2009年冬季在于都县进行了降香黄檀的育苗、栽植试验,结果表明,苗圃地及造林地苗木均遭受了不同程度的冻害,通过进行不同海拔和坡向的栽植试验,认为该树种目前不宜作造林树种进行大面积造林。  相似文献   
500.

Background

Ketoprofen is a non-steroidal anti-inflammatory drug which has been widely used for domestic animals. Orally administered racemic ketoprofen has been reported to be absorbed well in pigs, and bioavailability was almost complete. The objectives of this study were to analyze R- and S-ketoprofen concentrations in plasma after oral (PO) and intra muscular (IM) routes of administration, and to assess the relative bioavailability of racemic ketoprofen for both enantiomers between those routes of administration in growing pigs.

Methods

Eleven pigs received racemic ketoprofen at dose rates of 4 mg/kg PO and 3 mg/kg IM in a randomized, crossover design with a 6-day washout period. Enantiomers were separated on a chiral column and their concentrations were determined by liquid chromatography-tandem mass spectrometry. Pharmacokinetic parameters were calculated and relative bioavailability (Frel) was determined for S and R –ketoprofen.

Results

S-ketoprofen was the predominant enantiomer in pig plasma after administration of the racemic mixture via both routes. The mean (± SD) maximum S-ketoprofen concentration in plasma (7.42 mg/L ± 2.35 in PO and 7.32 mg/L ± 0.75 in IM) was more than twice as high as that of R-ketoprofen (2.55 mg/L ± 0.99 in PO and 3.23 mg/L ± 0.70 in IM), and the terminal half-life was three times longer for S-ketoprofen (3.40 h ± 0.91 in PO and 2.89 h ± 0.85 in IM) than R-ketoprofen (1.1 h ± 0.90 in PO and 0.75 h ± 0.48 in IM). The mean (± SD) relative bioavailability (PO compared to IM) was 83 ± 20% and 63 ± 23% for S-ketoprofen and R-ketoprofen, respectively.

Conclusions

Although some minor differences were detected in the ketoprofen enantiomer concentrations in plasma after PO and IM administration, they are probably not relevant in clinical use. Thus, the pharmacological effects of racemic ketoprofen should be comparable after intramuscular and oral routes of administration in growing pigs.  相似文献   
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