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采用细SephadexLH-20葡聚糖凝胶柱从小麦叶片提取液中分离纯化得到两种黄酮类化合物,经核磁共振和紫外分光光度计吸收光谱等分析技术,鉴定出其结构为异荭草甙和异荭草甙-7-O-阿拉伯糖—葡萄糖苷。将不同浓度的两种黄酮类化合物加入麦长管蚜全纯人工饲料中饲养麦长管蚜,结果表明,分离得到的两种黄酮类化合物对麦长管蚜的生长、发育和繁殖均有明显的抑制作用。其中异荭草甙对该蚜有明显的抗生性,其抗性阈值浓度为0.02%左右。 相似文献
513.
The use of selected plasma enzyme activities for the diagnosis of fatty liver-hemorrhagic syndrome in laying hens 总被引:8,自引:0,他引:8
Profiles of plasma enzymes were compared in two strains of single comb white leghorn laying hens, a normal commercial strain and strain UCD-003, which is highly susceptible to fatty liver-hemorrhagic syndrome. Plasma activity of lactate dehydrogenase (LDH), glutamate dehydrogenase (GDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatine kinase (CK) averaged 194 +/- 27, 4.0 +/- 2.8, 146 +/- 20, 1.0 +/- 1.0, and 1041 +/- 268 U/liter, respectively in normal birds. Activities of LDH, GDH, AST, and ALT, but not CK, were significantly higher in UCD-003 than in normal hens. A bimodal distribution of activities of all enzymes was found in the UCD-003 hens, with some birds showing activities comparable with those of the normal hens and others with values that were 2-10 times greater than those found in normal hens. These results are consistent with the extensive hepatic lesions observed in the UCD-003 strain of birds. Average gross hemorrhagic scores from visual inspection (scale of 0-3) were 0.28 +/- 0.45 in normal birds and 1.63 +/- 0.94 in the UCD-003 birds. Even though no clear relationship was found between plasma enzyme activities and the extent of liver hemorrhage in individual birds, the UCD-003 hens consistently had average values significantly higher for plasma enzymes that indicate liver damage. The results suggest that measurement of enzyme activities indicative of liver damage in birds, particularly AST, LDH, and GDH, is a valuable tool in the diagnosis of fatty liver-hemorrhagic syndrome in a flock of layers. 相似文献
514.
Saline drinking water in broiler and Leghorn chicks and the effect in broilers of increasing levels and age at time of exposure 下载免费PDF全文
Mirsalimi SM Julian RJ 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(7):413-417
The purpose of this research was to assess the effect of increasing levels of salt and 5 g/L of salt (0.20% extra sodium) in the drinking water in broiler chickens at various ages up to five weeks of age and to compare the response of broiler chickens and White Leghorns to saline water (0.20% sodium). The effect was measured by the response of the right ventricle to pulmonary hypertension. The results indicated that broiler chickens under three weeks are more susceptible to saline water containing 0.20% sodium than those over three weeks of age, and that broilers given increasing levels of dietary salt may be more resistant to excess dietary salt than those that have had no previous exposure. The results also demonstrated that broiler chickens are more susceptible than White Leghorns to 0.20% extra dietary sodium in drinking water. We conclude that Leghorn chicks are more resistant to excess dietary sodium than broilers and that broilers become more resistant to saline water containing 0.20% sodium after three weeks of age. 相似文献
515.
de Lisle GW Collins DM Loveday AS Young WA Julian AF 《New Zealand veterinary journal》1990,38(1):10-13
Mycobacterium bovis was isolated from diagnostic samples from 57 cats submitted to New Zealand Animal Health Laboratories from 1974 to 1986. With six exceptions, these cats came from suburban and rural areas of New Zealand where M.bovis was also present in feral and wild animals, especially the brush-tailed possum. Tuberculous skin lesions were seen in 33 (58%) of the cats. Histologically, these lesions had some similarities to those of cat leprosy. Included in the 57 cats was a group of 12 tuberculous animals which were diagnosed in a suburban veterinary practice over a 3 month period. When these 12 M. bovis isolates were examined by DNA restriction endonuclease analysis, they were found to be identical. This evidence, together with the relatively short period during which the cases occurred, suggested that these cats were exposed to a single source of infection. 相似文献
516.
Morley P Mathes M Guth A Dow S 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2008,22(4):886-892
BACKGROUND: Flow cytometry has been used to detect anti-red blood cell (RBC) antibodies in dogs with immune-mediated hemolytic anemia (IMHA), but the prevalence of anti-RBC antibodies in anemic and nonanemic dogs with a variety of different diseases has not been assessed previously. HYPOTHESIS: We hypothesized that anti-RBC antibodies would be more common in anemic dogs and in dogs with immune-mediated disorders and cancer. ANIMALS: Blood samples from 292 dogs were analyzed prospectively by flow cytometry for anti-RBC antibodies. METHODS: Blood samples from 147 anemic and 145 nonanemic dogs were evaluated by flow cytometry to detect surface-bound immunoglobulin (Ig) G and IgM antibodies on RBC. Disease associations with RBC antibodies were determined, as was the correlation between disease status and the percentage of Ig(+) RBC. The specificity and sensitivity of flow cytometry and clinical variables for the diagnosis of IMHA were compared by Bayesian analysis. RESULTS: Anemic dogs were significantly more likely to be positive for anti-RBC antibodies (IgG, IgM, or both) than nonanemic dogs. Anemic dogs also had significantly higher percentages of Ig(+) RBC than nonanemic dogs, whereas dogs with IMHA had significantly higher percentages of Ig(+) RBC than dogs with all other diseases. Dogs with IMHA, infectious diseases, and immune-mediated thrombocytopenia were significantly more likely to have anti-RBC antibodies than dogs with other medical or surgical diseases. CONCLUSIONS: Anemic dogs with immune-mediated diseases and infectious diseases were at the highest risk for the development of anti-RBC antibodies, and flow cytometry for the detection of IgG on RBC was highly sensitive and specific for the diagnosis of IMHA. 相似文献
517.
Elmslie RE Glawe P Dow SW 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2008,22(6):1373-1379
Background: Continuous administration of low doses of cyclophosphamide and standard doses of cyclooxygenase‐inhibiting drugs has been shown to suppress tumor angiogenesis, reverse immunosuppression, and deplete regulatory T cells in cancer models. Hypothesis: We hypothesized that continuous treatment with low‐dose cyclophosphamide and full‐dose piroxicam would delay tumor recurrence in dogs with soft tissue sarcomas (STS). Animals: Eighty‐five dogs with incompletely resected STS, 30 treated dogs, and 55 contemporary control dogs. Methods: Treatment outcomes in 85 dogs with incompletely resected STS were evaluated in a retrospective study. Dogs in the treatment group received continuously administered low‐dose cyclophosphamide (10 mg/m2) and standard dose piroxicam (0.3 mg/kg) therapy. Time to local tumor recurrence (disease‐free interval; DFI) was compared between the 30 treated dogs and 55 untreated control dogs matched for age and tumor site and grade. Results: DFI was significantly (P < .0001) prolonged for STS of all sites (trunk and extremity) in treated dogs compared with untreated control dogs. The DFI also was significantly longer in treated dogs when tumor site (trunk and extremity) was compared. Twelve treated dogs (40%) experienced mild toxicity (grade 1 and 2) at some point during treatment and 1 dog developed grade 4 cystitis. Every other day dosing was tolerated better than daily dosing. Conclusions: Metronomic therapy with cyclophosphamide and piroxicam was very effective in preventing tumor recurrence in dogs with incompletely resected STS. These findings suggest that further evaluation of this approach is warranted as adjuvant therapy in dogs with highly metastatic tumors such as osteosarcoma and melanoma. 相似文献
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