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71.
A thymic precursor to the NK T cell lineage 总被引:1,自引:0,他引:1
CD1d-restricted autoreactive natural killer (NK1.1+) T cells function as regulatory cells in various disease conditions. Using improved tetramer tracking methodology, we identified a NK1.1- thymic precursor and followed its differentiation and emigration to tissues by direct cell transfer and in situ cell labeling studies. A major lineage expansion occurred within the thymus after positive selection and before NK receptor expression. Surprisingly, cytokine analysis of the developmental intermediates between NK and NK+ stages showed a T helper cell TH2 to TH1 conversion, suggesting that the regulatory functions of NK T cells may be developmentally controlled. These findings characterize novel thymic and postthymic developmental pathways that expand autoreactive cells and differentiate them into regulatory cells. 相似文献
72.
Le Crouéour G Thépenier P Richard B Petermann C Ghédira K Zèches-Hanrot M 《Fitoterapia》2002,73(1):63-68
In addition to six cyclopeptide alkaloids lotusines A-F, a new compound lotusine G, was isolated from the root bark of Zizyphus lotus by centrifugal partition chromatography. 相似文献
73.
The chloroform extract of the aerial parts of Centaurea musimomum exhibited significant activity against Plasmodium falciparum. The phytochemical study of this extract gave seven native and four acetylated sesquiterpene lactones. 相似文献
74.
Kamel Hessini Mokhtar Lachaâl Cristina Cruz Abdelaziz Soltani 《Journal of plant nutrition》2013,36(5):821-836
ABSTRACT The present study was performed to characterize the interaction between nitrogen (N) form and availability with respect to growth, water relations, and mineral nutrition of wild swiss chard (Beta macrocarpa Guss). Plants were cultured hydroponically with two levels of N concentrations, high-N (2.5 mM) or low-N (0.5 mM), added as nitrate (NO? 3) or ammonium (NH+ 4). At high N, growth was affected significantly by N form. If the NO? 3 medium was considered as control, the use of NH+ 4 decreased dry matter production and leaf area by ca. 35%. Use of NH+ 4 led to water economy and did not affect the nutrient content of the plant tissues. Compared to growth with high N, plants growth fell in either low- NO? 3 or low- NH+ 4 medium. In this case, the difference between the two N sources was not significant. Our results showed that the replacement of NO? 3 by NH+ 4 as the N source decreased the NO? 3 concentration in consumable leaves and increased the water use efficiency. 相似文献
75.
Sarra Ouertatani Kamel Regaya John Ryan Azaiez Gharbi 《Journal of plant nutrition》2013,36(6):850-860
Faba beans (Vicia faba L) are grown in northern Tunisia where annual rainfall approaches 1200 mm and where the soil acidity is the most limiting factor for plant growth. Aluminum (Al) and manganese (Mn) toxicities provide a hostile environment to root growth. To alleviate such a problem, farmers use selected acid-tolerant species. However, crop yields remain far below their optimal levels. Liming, a practice to eliminate acidity, has never been tried in this area before. This research aimed to evaluate the impact of lime and mineral fertilizers on root nodulation, plant uptake and yield of faba beans using a pot experiment. Treatments were combinations of two rates of calcium carbonate with three rates of superphosphate and three rates of potassium sulfate. Liming produced significantly higher number and weight of nodules on roots as well as higher nitrogen, phosphorus, and potassium contents in plant tissue. Liming also increased shoot growth and bean yield. 相似文献
76.
Ajmi Larbi Kamel Gargouri Mohamed Ayadi Ali Ben Dhiab Monji Msallem 《Journal of plant nutrition》2013,36(14):2083-2094
Boron (B) foliar treatments (300 mg L?1 as Solubor DF) were applied at two different dates in 2006 and 2007, prior to flowering and just after fruit set, on olive (Olea europaea L.) trees with no visual symptoms of B deficiency. Leaf B level increased after the first application as compared to control (?B). After July treatment, leaf B levels in ?B and +B treated trees increased when compared to the first sampling date. Foliar B application did not significantly affect vegetative growth in either year. During the first year of study (considered as an “on year”), B application had no significant effect on several phenological characteristics including fruit set, yield oil contents and oil quality. In the second year (“off year”), B sprays improved blooming rate, which increased from 20% in ?B to 30% in + B treated trees, and olive yield, which increased by 27% in response to B. 相似文献
77.
In order to determine the role of follicle-stimulating hormone (FSH) on the resumption of ovarian function in cows early postpartum (PP), bovine follicular fluid (FF) was used to selectively suppress concentrations of FSH. Calves were removed from all cows within 24 hr of birth. Follicular fluid that was treated with charcoal to remove steroids (15 ml; n = 14) or serum (S) from an ovariectomized cow (15 ml, n = 14) was injected i.m. twice daily from days 1 to 10 PP. Blood samples were collected before each injection and frequent samples (every 15 min for 6 hr) taken on days 5 and 10 PP. Eight cows from each group (FF and S) were slaughtered on the morning of day 11 PP and pituitaries and ovaries collected. The remaining cows (n = 6) were observed for estrus. Treatment with FF delayed follicular growth (P less than 0.01), as evidenced by the largest follicle per cow observed at time of slaughter (3.6 +/- 0.42 vs 11.5 +/- 1.77 mm dia; FF vs S). The intervals from parturition to first estrus (P less than 0.11) and to first progesterone rise (25.3 +/- 1.97 vs 18.0 +/- 3.62 d; P less than 0.06) tended to be delayed by treatment with FF vs S. Many of the cows treated with S ovulated by day 10 PP, they were divided retrospectively into those that had ovulated by (n = 9) or after (n = 5) day 10 PP for analysis. Cows treated with FF had lower (P less than 0.05) and less variable (P less than 0.01) serum FSH concentrations while levels of luteinizing hormone (LH) tended (P less than 0.08) to be greater on days 5 and 10 PP. Follicular fluid decreased levels of FSH (P less than 0.001), but not LH (P less than 0.15), in the samples obtained twice daily compared to S-treated cows that did not ovulate by day 10 PP. Anterior pituitaries were dissociated, and cells from each cow were cultured in order to ascertain whether treatment with FF in vivo would affect gonadotropin secretion in vitro. Estradiol-17 beta (E) was incubated with pituitary cells to determine the effect of E on gonadotropin secretion from cells of PP cows, and to ascertain whether treatment with FF in vivo and with E in vitro would interact to affect secretion of FSH and LH in culture. After 2 d of incubation, cells were treated with 10(-9) M E or vehicle (1% ethanol).(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
78.
Mofeida Nassif J. Peter Brooke David B. A. Hutchinson Osman M. Kamel E. Anthony Savage 《Pest management science》1980,11(6):679-684
Approximately 350 people, the inhabitants of two villages in the Fayum district of Egypt, were individually dusted with 50 g of powder containing 2.5 or 5.0 g permethrin kg?1. The inhabitants of a third village were left untreated as a control. Before treatment, approximately two-thirds of the population of all three villages were infested with bodylice. Fourteen days after treatment, the permethrin dust at the lower strength reduced the infestation by 98.8% and at the higher strength by 100%. The dust containing 5 g permethrin kg?1 maintained a high level of residual control (80.5%) for at least 91 days. The other gave a lower level of control at this time. Urine samples, taken from subjects in each of the treated villages before and after dusting, were analysed for permethrin metabolites. Results indicated that the maximum amount of permethrin absorbed, orally, through the skin, or by inhalation, was 39 μg kg?1 body weight, 24 h after treatment. No residue was found 30 days and 60 days after treatment. It was concluded that there was a very substantial safety margin when permethrin dusts were used on man for bodylice control. 相似文献
79.
Populations of bodylice, Pediculus humanus humanus L., in Egypt have become resistant to DDT and BHC, but not to malathion. A single dust application of permethrin (1 % a.i.; cis: trans-ratio 25:75) rendered 98.9% of the treated population louse-free. Thirty-seven days after dusting 91.3% and at the end of the 98-day observation period 64.6% of the population were free of infestation; during the same period the proportion of infested individuals rose by 12.3% in a similar untreated village. Permethrin is ovicidal against bodylice, affects the population of the human flea, PulexirritansL., and ishighly effective against the rat flea, Xenopsyllacheopis(Rothsch.). 相似文献
80.
Shymaa A. El Badawy Aziza M. M. Amer Gehan M. Kamel Kamal M. Eldeib Peter D. Constable 《Journal of veterinary pharmacology and therapeutics》2019,42(4):452-460
Values for pharmacokinetic variables are usually obtained in healthy animals, whereas drugs are frequently administered to diseased animals. This study investigated cefquinome pharmacokinetics in healthy goats and goats with experimentally induced mastitis. Five adult lactating goats received 75 mg of cefquinome intramammary infusion using a commercially available product into one udder half in healthy goats and goats with clinical mastitis that was induced by intracisternal infusion of 100 cfu of Staphylococcus aureus ATCC 29213 suspended in 5 ml of sterile culture broth. Cefquinome concentrations were determined in plasma and skimmed milk samples using high‐performance liquid chromatography (HPLC). Pharmacodynamics was investigated using the California Mastitis Test and pH of milk. Experimentally induced mastitis significantly increased the California Mastitis Test score and pH, and decreased the maximal cefquinome concentration and shortened the half‐life in milk when compared to healthy goats. In conclusion, mastitis facilitated the absorption of cefquinome from the mammary gland of lactating goats and induced marked changes in milk pH, emphasizing the importance of performing pharmacokinetic studies of antimicrobial agents in infected animals. 相似文献