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61.
Three virus isolates (WG-3, -4, and -5) from chicks affected by baby chick nephropathy were orally inoculated into 1-day-old specific-pathogen-free chicks of lines PDL-1 and 15I. Additional chicks were orally inoculated with avian nephritis virus (ANV) strain G-4260. Chicks inoculated with isolates WG-3, -4, and -5 died between 2 and 6 days postinoculation (PI), with mortality ranging from 0% to 53.3%. Pathological findings in the dead chicks included nephrosis in chicks inoculated with WG-3, -4, and -5, and nephritis and visceral urate deposition in chicks inoculated with G-4260. The stability of the WG-5 isolate, as well as the size of the particles and the nucleic acid type, were also similar to those of the G-4260 strain. All of the examined chicks inoculated with WG-3, -4, and -5 had interstitial nephritis at 14 days PI. Therefore, the three virus isolates were considered to be ANV. However, there was no serological relationship between the isolates and ANV (G-4260 and M-8 strains). 相似文献
62.
Kondo H Kimoto H Shibuya H Shirai W Matsuo K Sato T 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2007,54(1):27-29
Uterine leiomyosarcoma occurring spontaneously in a domestic golden hamster was examined histologically and immunohistochemically. The histological findings for this tumour were similar to those for leiomyosarcomas described in other species. Immunohistochemical examination demonstrated the positivity of neoplastic cells with alpha-smooth muscle actin and desmin. From the results mentioned above, the tumour of this case was revealed to be of smooth muscle origin. To our knowledge, this is the first report of a uterine leiomyosarcoma in domestic golden hamsters. 相似文献
63.
The intra-abdominal tumor developing in the uterus and lung of a domestic Shorthair cat was examined histopathologically and immunohistochemically. The tumor showed a proliferation of both endometrial stromal and smooth muscle cells accompanied by prominent vasculature. There were well-differentiated endometrial glands, and tubuli made up a monolayer of eosinophilic cuboidal epithelium. Immunohistochemically, the spindle-shaped cells and half of the stromal-like cells reacted to caldesmon and desmin antibodies. The neoplastic epithelium expressed AE1/AE3 cytokeratin. Feline endometrial stromal tumor has, to the best of our knowledge, not been reported previously and has smooth muscle and glandular components that are a unique variant to the human counterpart. 相似文献
64.
Comparison of the diuretic effect of furosemide by different methods of administration in healthy dogs
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65.
Nagao K Okazaki R Nakamura T Miura YN Osawa T Bajo K Matsuda S Ebihara M Ireland TR Kitajima F Naraoka H Noguchi T Tsuchiyama A Yurimoto H Zolensky ME Uesugi M Shirai K Abe M Yada T Ishibashi Y Fujimura A Mukai T Ueno M Okada T Yoshikawa M Kawaguchi J 《Science (New York, N.Y.)》2011,333(6046):1128-1131
Noble gas isotopes were measured in three rocky grains from asteroid Itokawa to elucidate a history of irradiation from cosmic rays and solar wind on its surface. Large amounts of solar helium (He), neon (Ne), and argon (Ar) trapped in various depths in the grains were observed, which can be explained by multiple implantations of solar wind particles into the grains, combined with preferential He loss caused by frictional wear of space-weathered rims on the grains. Short residence time of less than 8 million years was implied for the grains by an estimate on cosmic-ray-produced (21)Ne. Our results suggest that Itokawa is continuously losing its surface materials into space at a rate of tens of centimeters per million years. The lifetime of Itokawa should be much shorter than the age of our solar system. 相似文献
66.
Fibril reconstruction process, that is, the nucleation and growth of mixed type I collagen fibril of shark and pig, progressed faster than that of the individual collagen species of shark or pig. The reconstructed mixed collagen fibril had a greater resistance to return to the solution or to melt into gelatin in comparison with the counterpart consisting solely of shark collagen. The denaturation temperature of the mixed collagen gel was about 10 degrees C higher than that of shark, and about 5 degrees C lower than that of pig. By scanning electron microscopy, the diameter of mixed collagen fibril showed an intermediate range between shark and pig collagen fibril. The breaking strength of the mixed collagen gel was tougher than that of pig, but weaker than that of shark. Other physicochemical properties of the mixed type I collagen gel were observed to be at intermediate positions between those of shark and pig type I collagen gels. 相似文献
67.
Makoto Shirai Shingo Arakawa Hiroaki Miida Takuya Matsuyama Junzo Kinoshita Toshihiko Makino Kiyonori Kai Munehiro Teranishi 《Journal of toxicologic pathology》2013,26(2):175-186
To assess modification of thioacetamide-induced hepatotoxicity in mice fed a high-fat
diet, male C57BL/6J mice were fed a normal rodent diet or a high-fat diet for 8 weeks and
then treated once intraperitoneally with thioacetamide at 50 mg/kg body weight. At 24 and
48 hours after administration, massive centrilobular hepatocellular necrosis was observed
in mice fed the normal rodent diet, while the necrosis was less severe in mice fed the
high-fat diet. In contrast, severe swelling of hepatocytes was observed in mice fed the
high-fat diet. In addition, mice fed the high-fat diet displayed more than a 4-fold higher
number of BrdU-positive hepatocytes compared with mice fed the normal rodent diet at 48
hours after thioacetamide treatment. To clarify the mechanisms by which the hepatic
necrosis was attenuated, we investigated exposure to thioacetamide and one of its
metabolites, the expression of CYP2E1, which converts thioacetamide to reactive
metabolites, and the content of glutathione S-transferases in the liver. However, the
reduced hepatocellular necrosis noted in mice fed the high-fat diet could not be explained
by the differences in exposure to thioacetamide or thioacetamide sulfoxide or by
differences in the expression of drug-metabolizing enzymes. On the other hand, at 8 hours
after thioacetamide administration, hepatic total glutathione in mice fed the high-fat
diet was significantly lower than that in mice fed the normal diet. Hence, decreased
hepatic glutathione amount is a candidate for the mechanism of the attenuated necrosis. In
conclusion, this study revealed that thioacetamide-induced hepatic necrosis was attenuated
in mice fed the high-fat diet. 相似文献
68.
Quang Dung Le Kotaro Shirai Duc Cu Nguyen Nobuyuki Miyazaki Takaomi Arai 《Water, air, and soil pollution》2009,204(1-4):69-78
In order to understand the present status of heavy metal contamination and the risk associated with human consumption, commercial freshwater eels in Vietnam were examined. The concentration of eight elements (Cr, Mn, Co, Cu, Zn, Sr, Cd, and Pb) was determined in muscle tissue of tropical eel Anguilla marmorata collected in four provinces, Quang Tri, Quang Ngai, Binh Dinh, and Phu Yen, from the central part of Vietnam. Concentrations of Cu, Zn, Cd, and Sr were found to be highest, whereas Co concentration showed the lowest at Quang Tri. Cr was recorded to be lowest in Quang Ngai, while the amounts of Mn and Pb did not vary among locations. Although the metal concentrations in the muscle of A. marmorata did not represent a risk for human consumption, elevated Cd concentrations in Quang Tri related to agriculture activities suggest that further investigations should be undertaken to clarify the potential risk to the eel population. 相似文献
69.
Higuchi T Shirai N Suzuki H 《饲料博览》2008,(9):38-38
先前报道从盐渍的鲱鱼卵产品(干鲱鱼卵)中提取的脂质含大量胆固醇,磷脂酰胆碱和n-3多不饱和脂肪酸(PUFAs),如二十碳五烯酸(EPA,20:5n-3)和二十二碳六烯酸(DHA,22:6n-3);该脂质可降低鼠血浆脂质和葡萄糖浓度。本试验旨在评价含大量蛋白的干鲱鱼卵对鼠脂质和葡萄糖代谢的影响。雄性Crij-CD-1(ICR)鼠分别采食3种含冻千鲱鱼卵的试验饲粮。12周。试验饲粮如下:无干鲱鱼卵(对照饲粮)、1%干鲱鱼卵(含1%干鲱鱼卵饲粮)和4%干鲱鱼卵(含4%干鲱鱼卵饲粮)。与对照饲粮组相比,1%和4%干鲱鱼卵饲粮组,血浆总胆固醇、磷脂和葡萄糖浓度趋向于降低。对照饲粮组和4%干鲱鱼卵饲粮组,血浆葡萄糖浓度有显著差异(P〈0.05)。4%干鲱鱼卵饲粮组血浆脂联素浓度显著高于对照饲粮组(P〈0.05),但是血浆胰岛素浓度在三组间没有显著差异。与对照饲粮组相比,4%干鲱鱼卵饲粮组肝脏总胆固醇和磷脂浓度趋于降低。与对照饲粮组相比,1%和4%干鲱鱼卵饲粮组血浆和肝脏n-3,n-6值极显著升高(分别为P〈O.005,P〈0.0005)。结果显示摄食干鲱鱼卵影响鼠脂质和葡萄糖代谢。 相似文献
70.
Su Myo Thwe Takeshi Kobayashi Tianyao Luan Takaaki Shirai Munenaka Onodera Naoko Hamada-Sato Chiaki Imada 《Fisheries Science》2011,77(2):279-288
γ-Aminobutyric acid (GABA)-producing lactic acid bacteria (LAB) were isolated from four types of Myanmar traditional fermented
fishery products with boiled rice. All of them belonged to the genus Lactobacillus, and comparison of the effects of these representatives on GABA accumulation in fermented fishery products with boiled rice
revealed that Lactobacillus farciminis D323 is the most effective strain as a starter culture. These results may contribute to the development of traditional fermented
fishery products with functional properties. In addition, this study is the first to show in detail the distribution of GABA-producing
LAB in Southeast Asian fermented fishery products. 相似文献