Evaluation of the effect of dietary lycopene, the main carotenoid in tomato (Lycopersicon esculentum), on the in vivo renal reducing ability by a radiofrequency electron paramagnetic resonance method     

Yoshida K  Yokoyama H  Oteki T  Matsumoto G  Aizawa K  Inakuma T 《Journal of agricultural and food chemistry》2011,59(7):2966-2971

Although it has been reported that dietary lycopene, the main carotenoid in tomato, improved drug-induced nephropathy, there are no reports on the effect of orally administered lycopene on the in vivo renal reducing (i.e., antioxidant) ability. The radiofrequency electron paramagnetic resonance (EPR) method is a unique technique by which the in vivo reducing ability of an experimental animal can be studied. In this study, the in vivo changes in the renal reducing ability of rats orally administered lycopene were investigated using a 700 MHz EPR spectrometer equipped with a surface-coil-type resonator. Rats were fed either a control diet or a diet containing lycopene. After 2 weeks, in vivo EPR measurements were conducted. The renal reducing ability of lycopene-treated rats was significantly greater than that of the control. This is the first verification of in vivo antioxidant enhancement via dietary lycopene administration.  相似文献   

Prevalence and intensity of <Emphasis Type="Italic">Phrixocephalus umbellatus</Emphasis> (Copepoda: Siphonostomatoida: Pennellidae) parasitic on <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis> in the western part of the Sea of Japan     

Susumu Ohtsuka  Kazuhibo Harada  Kazutaka Miyahara  Tatsuaki Nagahama  Kazuo Ogawa  Taro Ohta 《Fisheries Science》2007,73(1):214-216

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Rupture of the Gastrocnemius Muscle in Neonatal Thoroughbred Foals: A Report of Three Cases     

Fumio SATO  Ryo SHIBATA  Mitsumori SHIKICHI  Katsumi ITO  Harutaka MURASE  Takanori UENO  Hidehumi FURUOKA  Kazutaka YAMADA 《Journal of Equine Science》2014,25(3):61-64

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Effect of Gelatin Hydrogel Sheet Containing Basic Fibroblast Growth Factor on Proximal Sesamoid Bone Transverse Fracture Healing in the Horse     

Naoki Sasaki  Satoru Nishii  Kazutaka Yamada  Hidehumi Huruoka  Yasuhiko Tabata 《Journal of Equine Veterinary Science》2013

Our objective in this study was to examine the effect of gelatin hydrogel (GH) sheets containing basic fibroblast growth factor (bFGF) on healing of proximal sesamoid bone transverse fractures in the horse. Ten healthy adult Thoroughbreds were used. The lateral proximal sesamoid bone of the left forelimb and the medial proximal sesamoid bone of the right forelimb were osteotomized, while the horses were under general anesthesia, and subsequently repaired by lag screw fixation using a single 4.5-mm cortical screw. A GH sheet containing 100 μg of bFGF was then sutured to the synovial membrane adjacent to the osteotomized proximal sesamoid bone. In the control group, the fracture was fixed with a lag screw, and the articular capsule was sutured. Fracture healing was assessed by radiographic examination once a week for 16 weeks after the operation. Radiographic examination of bone healing revealed significantly lower demineralization of the fracture line in the GH sheet-treated group than in the control group. The rate of demineralization of the fracture line in the GH sheet-treated group was significantly lower than that in the control group at 2, 4, and 8 weeks after the operation. In this study, we demonstrated that the use of a GH sheet containing bFGF promotes healing of proximal sesamoid bone fracture in the horse. Therefore, it is believed that this treatment strategy would be useful for quick recovery from bone fracture in the horse.  相似文献   

Hyperferritinemia in Dogs with Splenic Hemangiosarcoma     

Seishiro CHIKAZAWA  Yasutomo HORI  Fumio HOSHI  Kazutaka KANAI  Naoyuki ITO  Seiichi HIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(11):1515-1518

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The effect of a gelatin β-tricalcium phosphate sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2, and platelet-rich plasma (PRP) on equine articular cartilage defect     

Nao Tsuzuki  Jong-pil Seo  Kazutaka Yamada  Shingo Haneda  Hidefumi Furuoka  Yasuhiko Tabata  Naoki Sasaki 《The Canadian veterinary journal. La revue veterinaire canadienne》2013,54(6):573-580

We evaluated the curative efficacy of a gelatin β-tricalcium phosphate (β-TCP) sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2 (BMP-2), and platelet-rich plasma (PRP) by insertion into an experimentally induced osteochondral defect. A hole of 10 mm diameter and depth was drilled in the bilateral medial femoral condyles of 7 thoroughbred horses, and into each either a loaded sponge (treatment) or a saline-infused β-TCP sponge (control) was inserted. After 16 weeks, defects were examined by computed tomography, macroscopic analyses, and histological analyses. The median subchondral bone density and macroscopic subscores for joint healing were significantly higher in the treatment legs (P < 0.05). Although there was no significant difference in total histological scores between groups, hyaline cartilaginous tissue was observed across a wider area in the treatment group. Equine joint healing can be enhanced by inserting a BMP-2-, MSC-, and PRP-impregnated β-TCP sponge at the lesion site.  相似文献   

Low-dose recombinant canine interferon-γ for treatment of canine atopic dermatitis: An open randomized comparative trial of two doses     

Kuniyoshi Yasukawa  Shiori Saito  Takuya Kubo  Yuya Shibasaki  Kayo Yamaoka  Hisae Hachimura  Tomoko Kuyama  Akiteru Amimoto  Tsuyoshi Kumata  Yuko Kitahara  Masahiko Takenaka  Hitoshi Matsumura  Takehiro Uno  Tomiya Uchino  Kazutaka Takehara  Kouji Nishida  Michiyo Kadoya  Masafumi Sato  Kaoru Kato  Kanako Matsumoto  Satoshi Saito  Tetsuya Shimoda 《Veterinary dermatology》2010,21(1):42-49

The purpose of this study was to investigate the minimum effective dose of recombinant canine interferon-γ (rCaIFN-γ) for the treatment of dogs with atopic dermatitis (AD). Thirty-four dogs with AD from 17 animal hospitals in Japan were administered half or one-fifth of the approved rCaIFN-γ dose of 10 000 units/kg, three times a week for 4 weeks, followed by once weekly for an additional 4 weeks. Pruritus, excoriation, erythema and alopecia were evaluated and scored by the investigators on weeks 2, 4, 6, 8 and 12. The efficacy rate (number of excellent cases + number of good cases/total number of cases) at week 8 in the 2000 units/kg group was 36.4% for pruritus, 36.4% for excoriation, 45.5% for erythema and 36.4% for alopecia. In contrast, in the 5000 units/kg group, the efficacy rate was 64.3% for pruritus, 57.1% for excoriation, 78.6% for erythema and 78.6% for alopecia. The efficacy rate of the 5000 units/kg group was high for all signs evaluated and comparable to that of the 10 000 units/kg group reported in a previous study. The results of this study showed that 2000 units/kg of rCaIFN-γ is less effective than 5000 units/kg to treat dogs with AD, and the efficacy of the 5000 units/kg dose is comparable to that of 10 000 units/kg at week 8.  相似文献   

Mobility of the forearm skeleton in the Asiatic black (Ursus thibetanus), brown (U. arctos) and polar (U. maritimus) bears     

Hayato AMAIKE  Motoki SASAKI  Nao TSUZUKI  Mitsunori KAYANO  Motoharu OISHI  Kazutaka YAMADA  Hideki ENDO  Tomoko ANEZAKI  Naoya MATSUMOTO  Rumiko NAKASHITA  Misako KUROE  Hajime TARU  Gen BANDO  Yuko IKETANI  Ryohei NAKAMURA  Nobutaka SATO  Daisuke FUKUI  Nobuo KITAMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1284

In several primates and carnivores, pronation/supination angles of the forearm skeleton were examined, and it is thought that a larger angle is useful to acquire dexterous behaviors in feeding and/or life style, including climbing. In this study, the pronation/supination angles in Asiatic black, brown and polar bears were nondestructively examined. These specimens were classified as adult or non-adult. Three or four carcasses of each group of Asiatic black and brown bears were used for CT analysis, whereas only one adult polar bear was used. The forearms were positioned within the gantry of a CT scanner in both maximally supinated and pronated states. Extracted cross-sectional CT images of two positions were superimposed by overlapping the outlines of each ulna. The centroids of the radii were detected, and then the centroid of each radius and the midpoint of a line which connects between both ends of the surface of each radius facing the ulna, were connected by lines to measure the angle of rotation as an index of pronation/supination. In adult brown and polar bears, the angles were smaller as compared with the other groups (Asiatic black and non-adult brown bears). Asiatic black and non-adult brown bears can climb trees, whereas adult brown bears and polar bears cannot. This suggests that the pronation/supination angle is related to arboreal activity in Ursidae.  相似文献   

Effect of compound of gelatin hydrogel microsphere incorporated with platelet-rich-plasma and alginate on sole defect in cattle     

Tsuzuki N  Seo JP  Yamada K  Haneda S  Tabata Y  Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(8):1041-1044

Platelet-rich plasma (PRP) was administered to cattle with hoof defects (10-mm drill hole) to promote sole defect healing. Two types of gels were prepared. One was a mixed gel [compound of gelatin microspheres (GM) incorporated with PRP and alginate] and was used for the test group, while the other was an alginate gel used for the control group. Each hole was filled with one of these gels. The drill hole depth, pressure pain, and hardness of the regenerated tissue were measured for 3 weeks after treatment. These measurements improved significantly in the test group than in the control group. The results suggest that a compound of GM incorporated with PRP and alginate had a hoof-regenerating effect in cattle.  相似文献   

A new behavioral test for detecting decline of age-related cognitive ability in dogs     

Miho Nagasawa  Aiko Yatsuzuka  Kazutaka Mogi  Takefumi Kikusui 《Journal of Veterinary Behavior: Clinical Applications and Research》2012,7(4):220-224

A simple test for detecting decline of cognitive ability in aged dogs is needed in modern society. We used a basic place-selection test to develop a new test to assess cognitive ability in dogs, using 25 house dogs. Three covered bowls were placed in front of the subject. One of them was the correct bowl, from which the subject was rewarded with a food treat when he/she chose it. If the subject made an incorrect selection, he/she was returned to the starting point and the trial was repeated. Cognitive ability was assessed by the number of errors needed to fulfill the criterion. We found a significantly high correlation between the dogs’ ages and the number of error trials, suggesting that age-related cognitive dysfunction may reliably be assessed by the documented place-learning test.  相似文献