Cytotoxicity for porcine islet cells by complement of six animal species     

Itakura S  Kato H  Wang PC  Matsunaga A  Jitsukawa T  Edamura K  Ohgawara H  Mochizuki M  Nishimura R  Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1107-1109

Complement-mediated cytotoxicity for porcine islet cells (PICs) was evaluated using sera of six animal species. Then soluble complement receptor type-1 (sCR1) as an anti-complement agent was added to those sera, and the changes in 50% hemolytic unit of complement serum (CH50) and cytotoxic effect of those sera on PICs were examined. All the sera except for that of pig showed cytotoxicity. However, the extent of toxicity was considerably different between species. In the rat and human serum, sCR1 significantly reduced CH50 and cytotoxicity, however in the dog serum, sCR1 had no suppressive effects. These results may suggest that complement contribute to humoral cytotoxicity for PICs as a main factor, and the compatibility of complement with PICs differs between animal species.  相似文献   

Molecular cloning of the feline telomerase reverse transcriptase (TERT) gene and its expression in cell lines and normal tissues     

Yazawa M  Okuda M  Uyama R  Nakagawa T  Kanaya N  Nishimura R  Sasaki N  Masuda K  Ohno K  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(5):573-577

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Effect of natural and synthetic retinoids on the proliferation and differentiation of three canine melanoma cell lines     

Ohashi E  Inoue K  Kagechika H  Hong SH  NakagawaTakayuki  Takahashi T  Mochizuki M  Nishimura R  Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(2):169-172

The effect of two natural retinoids and synthetic retinoids with or without retinoid synergists on the proliferation and differentiation of 3 melanoma cell lines were investigated in vitro. No retinoids showed significant growth inhibitory effect on these cell lines when used alone, however, cell differentiation and significant growth inhibition were observed when treated with a combination of retinoids and a retinoid synergist. This study may suggest that, though the cells showed low susceptibilities when retinoids were treated alone, the combination of retinoids and a retinoid synergist may be effective to control the growth of canine melanoma cell lines.  相似文献   

Structural effects of dinotefuran and analogues in insecticidal and neural activities     

Kiriyama K  Nishimura K 《Pest management science》2002,58(7):669-676

The insecticidal potencies of dinotefuran and analogues against the adult male American cockroach, Periplaneta americana (L) were measured by injection with or without metabolic inhibitors. The potency of dinotefuran was close to those of clothianidin and imidacloprid under the conditions used. The nerve-excitatory and nerve-blocking activities were measured with central nerve cords of P americana. The nerve-excitatory activity of dinotefuran was lower than that of imidacloprid, but was comparable with that of clothianidin. The nerve-blocking activity of dinotefuran was comparable with that of imidacloprid and slightly higher than that of clothianidin. Quantitative analyses showed that variations in the insecticidal activity were better correlated with variations in the nerve-blocking activity than with those in the nerve-excitatory activity when the contribution of the hydrophobic factor was allowed for.  相似文献   

Inhibitory effect of stromal cell derived factor-1 on the replication of divergent strains of feline immunodeficiency virus in a feline T-lymphoid cell line     

Endo Y  Goto Y  Nishimura Y  Mizuno T  Watari T  Hasegawa A  Hohdatsu T  Koyama H  Tsujimoto H 《Veterinary immunology and immunopathology》2000,74(3-4):303-314

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Diazepam potentiates the positive inotropic effects of histamine and forskolin in guinea-pig papillary muscles     

Hara  Kaigo  Minami  Watanabe  Gomi  Nishimura  Chugun  & Kondo 《Journal of veterinary pharmacology and therapeutics》1998,21(5):375-379

There have been diverse reports on the effects of diazepam on cardiac contractility. The purpose of this study was to examine whether diazepam modifies the inotropic response elicited by histamine on an isolated guinea-pig papillary muscle. The responses of electrically driven papillary muscle to histamine and cyclic AMP-related inotropic agents were recorded in the absence and in the presence of diazepam. Histamine and forskolin, which directly stimulate adenylate cyclase, significantly increased the contractile force in the papillary muscle in a concentration-dependent manner. A histaminergic H₂-receptor antagonist, cimetidine, but not a H₁-receptor antagonist, diphenhydramine, at 10 μ M produced a rightward shift in the concentration-response curve for histamine. Diazepam (10 μ M ) shifted the concentration-response curve for histamine and forskolin to the left by 1.8 and 1.6 times, respectively. Neither a central type (fulmazenil) nor a peripheral type (PK11195) of benzodiazepine receptor antagonist modified the effect of diazepam on the histaminergic-evoked contraction. Phosphodiesterase blockade by 3-isobutyl-1-methylxanthine shifted the concentration-dependent curve for histamine to the left. A combination of 3-isobutyl-1-methylxanthine also produced a leftward shift of the curve. However, there was no significant difference between the 3-isobutyl-1-methylxanthine only group and the combination group. These results indicate that diazepam potentiates the positive inotropic effect produced by histamine, probably mediated via an increase in cyclic AMP levels induced by histamine.  相似文献   

Quantitative structure-activity studies of substituted benzyl chrysanthemates: 7. Relationship between induction of repetitive discharges and effects on the action potential     

Masato Omatsu  Keiichiro Nishimura  Toshio Fujita 《Pesticide biochemistry and physiology》1985,24(2):192-199

The effects of substituted benzyl (1R)-trans-chrysanthemates and related compounds on the action potential of the crayfish giant axon were investigated using an intracellular microelectrode. The effects are broadly classified into three types: deceleration of the falling-phase of the action potential (type A), elevation of the depolarizing after-potential (type B), and the combination type (type C). The potency of the type A compounds to decelerate the rate of the falling-phase was determined in terms of the concentration required for reducing the rate to a specified degree. This potency was shown to correlate with the potency to induce repetitive discharges in the cockroach central nerve cord in terms of minimum effective concentration, which was determined previously. The potency of the types B and C compounds to elevate the after-potential was, however, not related directly with the cockroach nerve repetitive activity. The elevation of the after-potential is one of the critical factors but other effects such as a depolarization of the resting potential may be involved in determining the repetitive activity.  相似文献   

Comparison of the global gene expression profiles in the bovine endometrium between summer and autumn     

Ryosuke SAKUMOTO  Ken-Go HAYASHI  Shiori SAITO  Hiroko KANAHARA  Keiichiro KIZAKI  Kosuke IGA 《The Journal of reproduction and development》2015,61(4):297-303

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Comparison of the canine corneal epithelial cell sheets cultivated from limbal stem cells on canine amniotic membrane,atelocollagen gel,and temperature‐responsive culture dish          下载免费PDF全文

Eunryel Nam  Naoki Fujita  Maresuke Morita  Keiko Tsuzuki  Hsing Yi Lin  Cheng Shu Chung  Takayuki Nakagawa  Ryohei Nishimura 《Veterinary ophthalmology》2015,18(4):317-325

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Insecticidal and neuroblocking potencies of variants of the imidazolidine moiety of imidacloprid-related neonicotinoids and the relationship to partition coefficient and charge density on the pharmacophore     

Kagabu S  Ishihara R  Hieda Y  Nishimura K  Naruse Y 《Journal of agricultural and food chemistry》2007,55(3):812-818

The pharmacophore of the neonicotinoid insecticide imidacloprid, nitroiminoimidazolidine, was modified to heterocycles such as thiazolidine, pyrrolidine, dihydroimidazole, dihydrothiazole, and pyridone conjugated to nitroimine (=NNO2) or nitromethylene (=CHNO2). Their 6-chloro-3-pyridylmethyl or 5-chloro-3-thiazolylmethyl derivatives were examined for insecticidal activity against the American cockroach by injection and for neuroblocking activity using the cockroach ganglion. Most of the compounds having the neonicotinoidal pharmacophore exhibited insecticidal activity at the nanomolar level, which was enhanced in the presence of synergists, and high neuroblocking activity at the micromolar level. Quantitative analysis for the compounds showed that the neuroblocking potency is proportional both to the Mulliken charge on the nitro oxygen atom and to the partition coefficient log P value. The equation for the insecticidal versus neuroblocking potencies indicated that both potencies are related proportionally with each other when the other factors are the same.  相似文献