Arthroderma benhamiae infection in a rabbit   总被引：3，自引：0，他引：3  

Saito K  Kano R  Nakamura Y  Watanabe S  Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(8):929-931

The isolate from the rabbit with dermatophytosis which was transmitted to the owners was proved to be Arthroderma benhamiae (-) by mating experiments as well as by chitin synthase 1 (CHSI) gene analysis.  相似文献   

Identification of novel inhibitors of calling and in vitro [14C]acetate incorporation by pheromone glands of Plodia interpunctella     

Hirashima A  Eiraku T  Watanabe Y  Kuwano E  Taniguchi E  Eto M 《Pest management science》2001,57(8):713-720

Some octopamine agonists were found to suppress in vitro biosynthesis of the calling pheromone of the Indian meal moth, Plodia interpunctella. Isolated pheromone-gland preparations incorporated sodium [14C]acetate at a linear rate for 3 h when incubated with the pheromone biosynthesis activating neuropeptide (PBAN). This incorporation was dependent on the dose of PBAN (up to 0.5 microM). Thin-layer chromatography of a pheromone-gland extract revealed quantitative incorporation of radioactivity into a product exhibiting the same mobility as (Z,E)-9,12-tetradecadienyl acetate, the main component of the calling pheromone of P interpunctella. Twenty-seven octopamine agonists were initially screened using a calling behaviour bioassay of female P interpunctella. Four derivatives with activity in the nanomolar range were identified which were, in order of decreasing pheromonostatic activity: 2-(2,6-diethylphenylimino)thiazolidine > 2-(2,6-diethylphenylimino)oxazolidine > 2-(2,6-dimethylphenylimino)thiazolidine > 2-(2-ethylphenylimino)oxazolidine. These compounds also showed in vitro inhibitory activity in intracellular de novo pheromone biosynthesis. The results of the present study indicate that these derivatives could provide useful information in the characterization and differentiation of octopaminergic receptor types and subtypes.  相似文献   

Identification of Trichoderma SKT-1, a biological control agent against seedborne pathogens of rice     

Satoshi Watanabe  Kazuo Kumakura  Hideki Kato  Hiroyuki Iyozumi  Masayuki Togawa  Kozo Nagayama 《Journal of General Plant Pathology》2005,71(5):351-356

Trichoderma SKT-1 was previously reported as a powerful biological control agent against seedborne pathogens of rice, but the taxonomic disposition of the fungal isolate was not clear. Trichoderma SKT-1 produced irregular pyramidal warts on conidia and had an optimum growth temperature of 30°C. Morphological characteristics and colony growth were identical to those of known species of Trichoderma, including the newly recognized species T. asperellum. The 5.8S rDNA with the internal transcribed spacer (ITS) region (ca. 514 bp) of the fungus was compared with those of known species to determine the phylogenetic placement of the fungus. The length and sequence of the regions from Trichoderma SKT-1 were completely identical to those of an isolate of T. asperellum NRRL 5242 (AJ230669). On the basis of these results, we concluded that Trichoderma SKT-1 was T. asperellum.  相似文献   

Antimicrobial activity of some N-(arylalkyl)maleimides     

S. Watanabe  Y. Igarashi  K. Yagami 《Pest management science》1992,34(2):99-104

A series of N-(arylalkyl)maleimides was prepared for the reaction of maleic anhydride and N-(arylalkyl) amines, and their antimicrobial activities were examined. All compounds exhibited antibacterial activity against gram-positive bacteria such as Bacillus subtilis and Staphylococcus aureus. Almost all compounds exhibited antibacterial activity against the gram-negative bacterium, Escherichia coli, but were inactive against Pseudomonas aeruginosa. Activities against gram-positive bacteria were independent of the nature of the substituent on the benzene ring or the length of alkyl group, but that against gram-negative bacteria was influenced by these parameters. All N-(arylalkyl)maleimides showed activity against yeasts and mycelial fungi.  相似文献   

Molecular heterogeneity in clinical isolates of Malassezia pachydermatis from dogs     

Aizawa T  Kano R  Nakamura Y  Watanabe S  Hasegawa A 《Veterinary microbiology》1999,70(1-2):67-75

Molecular investigation of 16 strains, conventionally identified to be Malassezia pachydermatis, isolated from dogs in Japan was carried out by random amplification of polymorphic DNA (RAPD) and chitin synthase 2 (CHS2) gene sequence analyses. The RAPD band patterns of 13 clinical isolates were identical to that of standard strain of M. pachydermatis (CBS-1879). The other three clinical isolates were different from the standard strain of M. pachydermatis in RAPD patterns, and two of the three isolates were identical. About 620 bp genomic DNA fragments of the CHS2 gene were amplified from the same 16 clinical isolates of M. pachydermatis by polymerase chain reaction (PCR) and sequenced. The phylogenetic analysis of the nucleotide sequences of CHS2 gene fragments of the 16 clinical isolates revealed that the 13 strains were genetically very close to the standard strain of M. pachydermatis and the other two isolates were genetically close to the standard strain of M. furfur rather than M. pachydermatis. The remaining one isolate was phylogenetically distinct from all the seven Malassezia species reported so far.  相似文献   

Ultrastructural Changes in Calvarial Osteoblast Cytoskeleton after Prostaglandin E₂ Administration in Rats     

K. Yamakura  H. Watanabe  S. Abe  & T. Yanagisawa 《Anatomia, histologia, embryologia》2001,30(5):301-307

Recent studies on the cytoskeleton of osteoblasts have been made mainly using cultured cells. However, the morphology of cultured cells may be altered during subculture. Therefore, cytoskeletal changes of calvarial osteoblasts were investigated in situ by electron microscopy using the detergent perfusion method to preserve cell morphology as well as selectively observing the cytoskeleton in the presence of a high concentration of prostaglandin E2 (PGE2) in the calvarial periphery in rats. Rats were perfused with a mixture of Triton X-100 and glutaraldehyde, then the cytoskeleton was observed by transmission electron microscopy. In osteoblasts of the control group, thick bundles of microfilaments ran parallel to the long axis of the cells immediately below the cell membrane adjacent to the osteoid layer. In contrast, in the osteoblasts of the PGE2-administered group, the external morphology was changed to an asteroid or cubic shape, and thick bundles of microfilaments immediately below the cell membrane adjacent to the osteoid were not observed, although microfilament fibres, with a diameter of 5-6 nm, were observed in the cytoplasm.  相似文献   

Outbreak of canine parvovirus infection and its elimination in a closed beagle dog colony     

T Hirasawa  S Iwaki  K Watanabe  K Mikazuki  S Makino  Y Hayashi 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1987,34(8):598-606

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Early changes of the M-wave of the quadriceps femoris muscle due to denervation in dogs.     

K Haruta  M Sasaki  Y Miyazono  S Inada  S Watanabe 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1979,41(3):319-323

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Comparative and topographical anatomy of the fowl. 51. Peripheral course of the olfactory nerve in the fowl     

T Watanabe  M Yasuda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1968,30(5):275-279

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Efficacy of enamel matrix protein applied to spontaneous periodontal disease in two dogs     

Watanabe K  Kikuchi M  Okumura M  Kadosawa T  Fujinaga T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):1007-1010

Enamel matrix protein (EMP) was applied for regeneration of periodontal tissue in 2 dogs with spontaneous periodontal disease. Case 1 had bony resorption around the root and root apex of the maxillary fourth premolars. Case 2 had vertical resorption of bone between the mandibular first and second molars. A flap was formed in the buccal gingiva, and EMP was applied onto the surface of the exposed root. One or 4 months postoperatively, increased bone level and clinical attachment were recognized. EMP was therefore suggested to be effective to induce regeneration of periodontal tissues in the cases with periodontal disease.  相似文献