Characterization of canine caspase-3   总被引：1，自引：0，他引：1  

Sano J  Oguma K  Kano R  Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):563-567

The canine caspase-3 gene was cloned and sequenced. The canine caspase-3 cDNA clone was 1473 base pairs in length and encoded 277 amino acids. The predicted amino acid sequence showed 88.4%, 88.0%, 85.9%, 65.9% and 60.6% homology with that of human, pig, mouse, chicken and zebra fish caspase-3, respectively. The caspase-3 mRNA was confirmed to express in skin, lymph node, bone marrow, small intestine and lung from a healthy dog by RT-PCR analysis.  相似文献   

Expression analyses of sex steroid-regulated genes in neonatal rat hypothalamus     

Yonehara K  Suzuki M  Yamanouchi K  Nishihara M 《The Journal of reproduction and development》2003,49(6):547-552

Estrogen plays an important role in sexual differentiation of the brain in rats during the perinatal period. To elucidate molecular mechanisms underlying sexual differentiation of the brain, in this study we investigated genes differentially expressed between sexes or induced to express by estrogen in neonatal rat hypothalamus using DNA microarray analysis in combination with real-time RT-PCR. It was found that the levels of expression of the genes encoding glutamic acid decarboxylase 65 and coronin 1b were higher in male than female hypothalamus on postnatal day (PN) 5 and those of collagen type 3 alpha1 and thioredoxin reductase 2 genes in female hypothalamus on PN5 were decreased and increased, respectively, by treatment with estradiol on PN2. Then the developmental changes in the expression of these 4 genes were examined from 1 day before the parturition to PN9, and they all showed sexual dimorphic patterns. In addition, dependence of the expression of these genes on either estradiol, testosterone or dihydrotestosterone during the neonatal period was confirmed. These results suggest that these four genes are involved in sexual differentiation of the rat brain, and that androgen per se as well as estrogen may take part in the processes.  相似文献   

Plectosporium blight of pumpkin and ranunculus caused by Plectosporium tabacinum     

Toyozo Sato  Tadaoki Inaba  Mitsutaka Mori  Ken Watanabe  Keisuke Tomioka  Etsuji Hamaya 《Journal of General Plant Pathology》2005,71(2):127-132

Based on inoculation experiments and morphological studies on the pathogenic isolates of Plectosporium spp., Microdochium blight (Hakuhan-byo in Japanese) of pumpkin (Cucurbita maxima Duch.) occurring in Japan was reconfirmed to be caused by Plectosporium tabacinum, and seedling blight (Kabugare-byo in Japanese) of ranunculus (Ranunculus asiaticus L.) was demonstrated to be caused by P. tabacinum. Both diseases are renamed Plectosporium blight in this article. Some isolates of the fungus appeared to have host specificity, whereas the others had either weak or no pathogenicity to pumpkin and ranunculus.  相似文献   

Permeability of mouse oocytes and embryos at various developmental stages to five cryoprotectants     

Pedro PB  Yokoyama E  Zhu SE  Yoshida N  Valdez DM  Tanaka M  Edashige K  Kasai M 《The Journal of reproduction and development》2005,51(2):235-246

To assess the permeability of mouse oocytes and embryos, matured oocytes and embryos at various stages of development were placed in five cryoprotectant solutions at 25 C for 25 min. From the cross-sectional areas of the oocytes/embryos, the relative change in volume was analyzed. In oocytes, shrinkage was least extensive and recovery was quickest in the propylene glycol solution, showing that propylene glycol permeates the oocytes most rapidly. Dimethyl sulfoxide, acetamide, and ethylene glycol permeated the oocytes slightly more slowly than propylene glycol. The oocytes in glycerol shrunk extensively and then expanded marginally, indicating slow permeation. The volume changes of 1-cell and 2-cell embryos were similar to those of oocytes, showing little change in permeability. In 8-cell embryos, the volume recovered much faster than in the earlier stages especially in glycerol and acetamide. In morulae, the volume recovery was much faster in glycerol and in ethylene glycol; in ethylene glycol, the extent of shrinkage was small and the recovery was fast, indicating an extremely rapid permeation. Although the permeability of oocytes/embryos generally increased as embryo development proceeded, the degree of increase varied greatly among the cryoprotectants. Interestingly, the volume change in propylene glycol was virtually unaffected by the stage of development. Such information will be valuable for determining a suitable protocol for the cryopreservation of oocytes/embryos at different stages of development.  相似文献   

A case study of infection of rice roots by deleterious fungi and minor pathogenic<Emphasis Type="Italic"> Pythium</Emphasis> species in a paddy field     

Hiromitsu Furuya  Keisuke Tubaki  Tsutomu Matsumoto  Shin-ichi Fuji  Hideki Naito 《Journal of General Plant Pathology》2007,73(1):8-14

Roots of rice plants grown in paddy fields in a transplant culture system were collected seven times between 8 and 22 weeks after transplanting, for two crop seasons. Rice seedlings grown in a potting medium amended with the collected roots were significantly shorter than those grown in the same medium either without the addition of the roots or amended with pasteurized, collected roots indicating that seedlings were inhibited by heat-labile microorganisms on the collected rice roots. Ninety-five and 172 pure cultures of Pythium spp. and fungi, respectively, were isolated from the rice roots collected 5 or 7.5 weeks after transplanting in the fields. Among these microorganisms, Pythium aristosporum inhibited seedling growth in greenhouse experiments, and Acremonium sp., Alternaria sp., Epicoccum nigrum, Fusarium sp., Massarina sp., Penicillium spp., Rhinocladiella sp., Stemphylium sp., Trichocladium sp., and several unidentified fungi inhibited seminal root growth in in vitro experiments. These microorganisms might be involved in the inhibition of seedling growth in soils amended with the rice roots collected from the paddy field. Thus, roots of rice plants at the middle stage of growth transplanted into paddy fields can harbor pathogenic or deleterious fungi or Pythium sp(p). The effects of these microorganisms on rice growth in paddy fields are discussed.  相似文献   

Idiopathic thrombocytopenia in Japanese black cattle     

Yasuda J  Okada K  Sato J  Sato R  Tachibana Y  Takashima K  Naito Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(1):87-89

A female Japanese Black cow showed hemorrhage under the mucous membrane of the eye conjunctiva and the vagina, and thrombocytopenia in peripheral blood. The clinical manifestations repeated spontaneously during long-term observation. The clinical findings were stable except for severe periodic bleeding. Prothrombin time, activated partial thromboplastin time and fibrinogen levels were normal. The anti-platelet antibody in the serum was analyzed by indirect platelet coagulation tests. Because the platelets in the micro-plate wells of the subject series showed coagulation up to 1:128, it was judged that anti-platelet antibody was present in the serum. A number of megakaryocytes were recognized in smear samples obtained from bone marrow of the scapula immediately after euthanasia. Therefore, this case was diagnosed as idiopathic thrombocytopenia.  相似文献   

Rhizoctonia Blight of Yacon Caused by Rhizoctonia solani AG-1 (IB)     

Keisuke TOMIOKA  Jun TAKEUCHI  Toyozo SATO  Tateo NAKANISHI 《Journal of General Plant Pathology》2002,68(1):103-104

Severe blight of stems and leaves caused by Rhizoctonia solani AG-1 (IB) was found on yacon (Smallanthus sonchifolius), a Compositae tuber crop, grown in Ehime Prefecture, Japan, from August to September 1996 and 1999. We named it “Rhizoctonia blight of yacon” as a new disease. Received 15 October 2001/ Accepted in revised form 25 November 2001  相似文献   

Genetic Analysis of Pathogenicity and Host-specific Toxin Production of Alternaria alternata Tomato Pathotype by Protoplast Fusion     

SALAMIAH  Yukitaka FUKUMASA-NAKAI  Hajime AKAMATSU  Hiroshi OTANI  Keisuke KOHMOTO  Motoichiro KODAMA 《Journal of General Plant Pathology》2001,67(1):7-14

Several pathotypes of Alternaria alternata are known to produce host-specific toxins (HSTs) as agents of pathogenicity or virulence. However, investigations into the genetic controls of HST biosynthesis and pathogenicity of Alternaria pathogens have been limited by the lack of a sexual stage in the life cycle of these pathogens. We report here the development of a protoplast fusion system and its use for genetic analysis of HST production and specific pathogenicity of the tomato pathotype of A. alternata that produces AAL-toxin as a HST. Drug-resistant transformants have been isolated by genetic transformation of nonpathogenic A. alternata (strain O-94) and A. alternata tomato pathotype (strain As-27) with vectors conferring resistance to hygromycin B and geneticin, respectively. Protoplasts of the respective transformants were fused by polyethylene glycol treatment or electrofusion. Fusion products were selected by culturing in the presence of both hygromycin B and geneticin, then confirmed by amplification using a polymerase chain reaction with specific primers to the transforming drug-resistance genes. Stable fusants were purified by successive subcultures on selective medium and single-spore isolation. The resultant stable fusants, probably inter-strain hybrids, had the same pathogenicity and toxin production as the wild-type strain As-27. These results suggest that protoplast fusion has potential applications for genetic analysis of A. alternata pathogens. Received 8 September 2000/ Accepted in revised form 27 October 2000  相似文献   

Assessment of left ventricular longitudinal function in cats with subclinical hypertrophic cardiomyopathy using tissue Doppler imaging and speckle tracking echocardiography     

Keisuke SUGIMOTO  Yoko FUJII  Hiroshi SUNAHARA  Takuma AOKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1101-1108

Hypertrophic cardiomyopathy (HCM) in cats is characterized by concentric left ventricular (LV) hypertrophy and both diastolic and systolic dysfunction. Although impaired cardiac function detected by tissue Doppler imaging (TDI) in cats with HCM was previously reported, reference ranges of TDI in normal cats and cats with HCM have been reported as widely variable. Two-dimensional speckle tracking echocardiography (STE) was useful for assessment of cardiac function in human patients with HCM, but clinical utility was not known in cats. The aim of this study was to assess global and segmental LV myocardial function using STE in cats with HCM whose TDI variables were within the reference range. A total of 35 cats of different breeds were enrolled in this study. The HCM group (n=22) was cats diagnosed as HCM without left atrial enlargement and with normal TDI measurements. HCM cats were further divided into a segmental hypertrophy (S-HCM) group and a diffuse hypertrophy (D-HCM) group. The control group consisted of 13 clinically healthy cats. No cats in any group showed any clinical symptoms. Conventional echocardiography, TDI, and global and segmental STE indices were evaluated and compared between groups. Only the longitudinal strain rate during early diastole was significantly decreased in both HCM groups, even in all segments including those without hypertrophy in S-HCM group. This study suggests that STE parameters are the more sensitive variables compared with conventional TDI parameters to detect early myocardial diastolic dysfunction in cats with HCM.  相似文献   

Addition of D-penicillamine,hypotaurine, and epinephrine (PHE) mixture to IVF medium maintains motility and longevity of bovine sperm and enhances stable production of blastocysts in vitro     

Sung-Sik KANG  Keisuke KOYAMA  Weiping HUANG  Yinghua YANG  Yojiro YANAGAWA  Yoshiyuki TAKAHASHI  Masashi NAGANO 《The Journal of reproduction and development》2015,61(2):99-105

The present study aimed to establish an efficient system for bovine embryo production by in vitro fertilization (IVF) that can achieve stable normal fertilization and blastocyst developmental rates in any bull without optimization of the sperm concentration in IVF medium. We examined the effects of a PHE mixture (20 μM D-penicillamine, 10 μM hypotaurine and 1 μM epinephrine), theophylline (2.5 mM), and sperm concentration (1, 2 or 5 × 10⁶ cells/ml) on fertilization and blastocyst developmental rates. High cleavage rates (78.3 to 92.4%) and blastocyst developmental rates (31.9 to 62.0%) at day 7 were obtained in the presence of PHE and theophylline in IVF medium with a sperm concentration of 2 × 10⁶ cells/ml using sperm from 9 bulls. In addition, the synergistic effect of PHE and theophylline on normal fertilization (2 pronuclei) was clarified at 12 h after IVF with a sperm concentration of 1 × 10⁶ cells/ml. Moreover, high linearity, high flagellar beat cross frequency, and low amplitude of lateral head of motile sperm were found by computer-assisted sperm analysis. In conclusion, the combination of the PHE mixture and theophylline synergistically accelerates sperm motility and sperm penetration of bovine oocytes. Theophylline activates sperm motility with increasing intracellular cAMP. However, PHE prevents an excessive increase of cAMP and maintains sperm motility without hyperactivation. When the combination of PHE and theophylline is added to IVF medium at a sperm concentration of 2 × 10⁶ cells/ml, we can achieve stable normal fertilization and blastocyst development in any bull.  相似文献