全文获取类型
收费全文 | 263篇 |
免费 | 7篇 |
专业分类
林业 | 48篇 |
农学 | 5篇 |
18篇 | |
综合类 | 12篇 |
农作物 | 25篇 |
水产渔业 | 21篇 |
畜牧兽医 | 99篇 |
园艺 | 3篇 |
植物保护 | 39篇 |
出版年
2022年 | 5篇 |
2021年 | 17篇 |
2020年 | 3篇 |
2019年 | 3篇 |
2018年 | 10篇 |
2017年 | 4篇 |
2016年 | 7篇 |
2015年 | 26篇 |
2014年 | 15篇 |
2013年 | 19篇 |
2012年 | 16篇 |
2011年 | 27篇 |
2010年 | 16篇 |
2009年 | 14篇 |
2008年 | 21篇 |
2007年 | 12篇 |
2006年 | 11篇 |
2005年 | 11篇 |
2004年 | 8篇 |
2003年 | 7篇 |
2002年 | 6篇 |
2001年 | 5篇 |
2000年 | 4篇 |
1998年 | 2篇 |
1993年 | 1篇 |
排序方式: 共有270条查询结果,搜索用时 46 毫秒
71.
Kazuhiro TAMURA Mikihiro YOSHIE Keisuke HASHIMOTO Eiichi TACHIKAWA 《The Journal of reproduction and development》2014,60(6):447-453
Angiogenesis in the developing corpus luteum (CL) is a prerequisite for establishment and maintenance of an early pregnancy. To explore the physiological significance of insulin-like growth factor-binding protein-7 (IGFBP7) in the developing CL, the effects of IGFBP7 on vascular endothelial growth factor (VEGFA)- and luteinizing hormone (LH)-induced in vitro tube formation were tested using isolated luteal microvascular endothelial cells (LECs). Capillary-like tube formation of LECs and their proliferation were stimulated by both VEGFA and LH. IGFBP7 treatment suppressed VEGFA- or LH-induced tube formation. The proliferation and migration of LECs, and phosphorylation of mitogen-activated protein kinase kinase and extracellular signal-regulated kinase 1/2 were inhibited by IGFBP7. Furthermore, IGFBP7 attenuated VEGFA-enhanced cyclooxygenase (COX)-2 mRNA expression and prostaglandin E2 secretion. These findings suggest the possibility that
luteal IGFBP7 secretion may suppress the stimulatory effect of VEGFA on angiogenesis in the early CL. 相似文献
72.
73.
Keisuke Maeda Fumika Yamamoto Masanari Toyoshi Masakazu Irie 《Animal Science Journal》2014,85(4):427-434
This study aimed to evaluate the effects of dietary lysine/protein ratio and fat levels on the growth, carcass characteristics and meat quality of finishing pigs fed feed made from food waste, including noodles and chocolate. Four dietary treatments, 2 levels of lysine/protein ratio (0.035 and 0.046) and 2 levels of fat (3.3% and 6.0%), were adapted to a 2 × 2 factorial arrangement. Each diet for the finishing pigs contained the same levels of adequate crude protein (16%) and lysine (0.58–0.75%), and similar levels of high total digestible nutrients (90.2–92.6%). In total, 32 LWD pigs with an average body weight of 57.2 kg were assigned to 4 dietary groups. The pigs were slaughtered at about 115 kg. Growth performance was not influenced by the dietary treatments. Carcass characteristics were slightly influenced by the dietary fat level. As the dietary lysine/protein ratio decreased, the marbling score of Longissimus dorsi muscle increased and the intramuscular fat (IMF) increased from 6.82% to 9.46%. Marbling score was not significantly influenced by the dietary fat level. These results indicate that IMF increased without adverse effects on growth, carcass characteristics and meat quality, when pigs were fed a diet with low lysine/protein ratio. 相似文献
74.
Yamaguchi A Clayton RN Mayeda TK Ebihara M Oura Y Miura YN Haramura H Misawa K Kojima H Nagao K 《Science (New York, N.Y.)》2002,296(5566):334-336
Eucrites are a class of basaltic meteorites that share common mineralogical, isotopic, and chemical properties and are thought to have been derived from the same parent body, possibly asteroid 4 Vesta. The texture, mineralogy, and noble gas data of the recently recovered meteorite, Northwest Africa (NWA) 011, are similar to those of basaltic eucrites. However, the oxygen isotopic composition of NWA011 is different from that of other eucrites, indicating that NWA011 may be derived from a different parent body. The presence of basaltic meteorites with variable oxygen isotopic composition suggests the occurrence of multiple basaltic meteorite parent bodies, perhaps similar to 4 Vesta, in the early solar system. 相似文献
75.
How nitrogen (N) cycling is regulated and how environmental change affects it are major study questions in forest ecology,
because N availability often limits the primary production of plants in many forest ecosystems. These are being extensively
highlighted because of growing concerns regarding chronic and elevated N deposition in forest ecosystems on a global scale.
Until now, N cycling has been mainly documented in association with various environmental factors other than microbial communities.
However, with the recent rapid development in culture-independent molecular-based techniques, microbial ecologists have discovered
that alterations in N cycling are highly associated with alternations in microbial communities through changes in either resource
supplies or processing rates. In this review, we describe nitrification as a key N cycling process and present general approaches
to associate the nitrification process with the nitrifying community in forest soils. Furthermore, we briefly summarize currently
available information about the relationship between the process and nitrifying community dynamics in soil. We suppose that
linking N cycling processes with microbial community dynamics provides a deeper insight into the mechanisms regulating N cycling
in forest ecosystems. 相似文献
76.
Keisuke Tomioka Junji Nishikawa Jouji Moriwaki Toyozo Sato 《Journal of General Plant Pathology》2011,77(1):60-63
Severe spotting and blight of leaves caused by Colletotrichum destructivum were found on snapdragon (Antirrhinum majus L.), a scrophulariaceous ornamental, in open fields in Shizuoka Prefecture, Japan, from June through September 2004. The
fungus is added to the group of the pathogens causing anthracnose of snapdragon. 相似文献
77.
Hafez E Takahashi T Ogawa H Sato M Nakai T Takasu C Uehara H Izumi K 《Journal of toxicologic pathology》2011,24(4):187-193
Diabetes mellitus (DM) and obesity are believed to be risk factors for colorectal cancer
in humans. In experiment 1, male nondiabetic Long-Evans Tokushima Otsuka (LETO) rats and
Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a model animal of type 2 DM, were
whole-body X-irradiated (4 Gy) at 6 and 8 weeks of age and euthanized at 78 weeks of age
(n=15, respectively). The incidences of small intestine adenocarcinoma in LETO and OLETF
rats were 0% and 30%, respectively. In experiment 2, male LETO and OLETF rats (n=24,
respectively) were given s.c. injections of 15 mg/kg azoxymethane (AOM) once weekly for 3
weeks and euthanized at 36 weeks of age. The incidences of Zymbal gland tumors in LETO and
OLETF rats were 0% and 67%, respectively (P<0.001), whereas those of
small intestine adenocarcinoma were 0% and 43% (P<0.001) and those of
cecum/colon adenocarcinoma were 46% and 79% (P<0.05), respectively.
Fatty change of hepatocytes was common in OLETF rats (63%) but not in LETO rats. Serum
triglyceride and free fatty acid levels in OLETF rats were significantly higher than in
LETO rats at sacrifice, whereas serum insulin levels in OLETF rats were very diverse.
These data suggest that hyperlipidemia plays a significant role in high susceptibility to
lower intestinal tract carcinogenesis in OLETF rats; this strain is susceptible to
AOM-induced Zymbal gland carcinogenesis. 相似文献
78.
Aoshima K Sunden Y Ishida S Ochiai K Umemura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1303-1308
Intrathecal (IT) immunization involves injecting antigens directly into the intraventricular or subarachnoid spaces, or brain, to induce antigen-specific antibodies (Ab) in the cerebrospinal fluid (CSF). In the present study, rabbits were immunized IT with inactivated rabies virus to investigate the origins of CSF Ab. The time course of Ab induction and tumor necrosis factor-alpha expression suggested the possibility that the CSF Ab originated in the serum. In addition, Ab-producing cells infiltrated around the blood vessels of the brain, suggesting local production of Ab within the central nervous system (CNS). Furthermore, subcutaneous (SC) immunization prior to IT immunization induced a rapid and magnified Ab response in the CSF compared with IT immunization alone. These results were confirmed by the fact that mice immunized SC prior to IT were more resistant to intracerebral challenge with rabies virus than mice immunized via the IT route alone. Taken together, these results suggest that combined SC and IT immunization is a more effective vaccination protocol for prophylaxis and treatment of rabies. 相似文献
79.
Wataru FUJII Asuka ONUMA Shin YOSHIOKA Keisuke NAGASHIMA Koji SUGIURA Kunihiko NAITO 《The Journal of reproduction and development》2015,61(6):589-593
The generation efficiencies of mutation-induced mice when using engineered zinc-finger nucleases (ZFNs) have
been generally 10 to 20% of obtained pups in previous studies. The discovery of high-affinity DNA-binding
modules can contribute to the generation of various kinds of novel artificial chromatin-targeting tools, such
as zinc-finger acetyltransferases, zinc-finger histone kinases and so on, as well as improvement of reported
zinc-finger recombinases and zinc-finger methyltransferases. Here, we report a novel ZFN pair that has a
highly efficient mutation-induction ability in murine zygotes. The ZFN pair induced mutations in all obtained
mice in the target locus, exon 17 of aminopeptidase Q gene, and almost all of the pups had biallelic
mutations. This high efficiency was also shown in the plasmid DNA transfected in a cultured human cell line.
The induced mutations were inherited normally in the next generation. The zinc-finger modules of this ZFN pair
are expected to contribute to the development of novel ZF-attached chromatin-targeting tools. 相似文献
80.
Takuya HIROSE Takayuki OGURA Keisuke TANAKA Jun MINAGUCHI Takeshi YAMAUCHI Toshiyuki FUKADA Yoh-ichi KOYAMA Kazushige TAKEHANA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1385-1389
Ehlers-Danlos syndrome (EDS) is a group of disorders caused by abnormalities that are
identified in the extracellular matrix. Transforming growth factor-β1 (TGF-β1) plays a
crucial role in formation of the extracellular matrix. It has been reported that the loss
of function of zinc transporter ZRT/IRT-like protein 13 (ZIP13) causes the spondylocheiro
dysplastic form of EDS (SCD-EDS: OMIM 612350), in which dysregulation of the TGF-β1
signaling pathway is observed, although the relationship between the dermis abnormalities
and peripheral TGF-β1 level has been unclear. We investigated the characteristics of the
dermis of the Zip13-knockout (KO) mouse, an animal model for SCD-EDS.
Both the ratio of dermatan sulfate (DS) in glycosaminoglycan (GAG) components and the
amount of collagen were decreased, and there were very few collagen fibrils with diameters
of more than 150 nm in Zip13-KO mice dermis. We also found that the
TGF-β1 level was significantly higher in Zip13-KO mice serum. These
results suggest that collagen synthesis and collagen fibril fusion might be impaired in
Zip13-KO mice and that the possible decrease of decorin level by
reduction of the DS ratio probably caused an increase of free TGF-β1 in
Zip13-KO mice. In conclusion, skin fragility due to defective
ZIP13 protein may be attributable to impaired extracellular matrix
synthesis accompanied by abnormal peripheral TGF-β homeostasis. 相似文献