Giardia duodenalis in colony stray cats from Italy     

Isabel Guadano Procesi  Azzurra Carnio  Federica Berrilli  Margherita Montalbano Di Filippo  Alessia Scarito  Cristina Amoruso  Marco Barni  Marco Ruffini  Giulia Barlozzari  Manuela Scarpulla  Claudio De Liberato 《Zoonoses and public health》2022,69(1):46-54

Giardia duodenalis is the most common intestinal protozoan in humans and animals worldwide, including eight morphologically identical assemblages, infecting pets, livestock, wildlife and human beings. Assemblages A and B are those with the higher zoonotic potential, and they have been detected in several mammals other than humans; the others (C to H) show a higher host specificity. Cats can harbour both the specific Assemblage F and the zoonotic ones A and B. Several studies have been carried out on G. duodenalis genotypes in cats; however, the role of this species in the epidemiology of giardiasis is still poorly understood. In this scenario, the present study carried out the detection and genetic characterization at sub-assemblage level of G. duodenalis from colony stray cats in central Italy. In the period 2018–2019, 133 cat faecal samples were analysed for the presence of G. duodenalis cysts by a direct immunofluorescence assay. Positive samples were subsequently subjected to molecular analyses for assemblage/sub-assemblage identification. Forty-seven samples (35.3%) were positive for G. duodenalis cysts by immunofluorescence. G. duodenalis DNA was amplified at SSU-rDNA locus from 39 isolates: 37 were positive for zoonotic Assemblage A and 2 showed a mixed infection (A + B). Positive results for the β-giardin gene were achieved for 25 isolates. Sequence analysis revealed 16 isolates belonging to Sub-assemblage AII and 8 to Sub-assemblage AIII. One isolate resulted as ambiguous AI/AIII. Large sequence variability at the sub-assemblage level was detected, with several double peaks and mutations, making complex a proper isolate allocation. When compared with previous studies, the 35.3% prevalence of G. duodenalis in cats reported in the present article was surprisingly high. Moreover, all positive cats resulted to be infected with zoonotic assemblages/sub-assemblages, thus indicating stray cats as a possible source of human giardiasis and highlighting the sanitary relevance of cat colonies in the study area.  相似文献   

Forest soils research and changing societal needs and values     

W.W. McFee  J.M. Kelly 《Forest Ecology and Management》2005,220(1-3):326-330

The 10th North American Forest Soils Conference was held in Sault Ste. Marie, Canada, 20–24 July 2003. These conferences, held at 5-year intervals since 1958, are intended to provide a forum to discuss and summarize the state of knowledge on selected topics related to forest soils. Approximately 60 papers were presented on a variety of topics related to understanding and managing soils that support forests with particular emphasis given to field research, soil carbon, and site management/classification. We discuss the topics of this conference in relation to earlier ones and conclude that advances are made unevenly and that there is a need for new approaches to investigating many of the topics. We note the change in forest management that has occurred since the first of these conferences and the resulting changes needed in our approach to research and management. The need for interaction of scientists and clientele groups is noted as necessary for development of sound management and research policy. The interaction of scientific disciplines is needed to attack problems that have been difficult to elucidate. The challenge to forest soil scientists is to mesh their scientific interests with the needs of society to obtain the resources needed to advance science and provide information that will improve forest management.  相似文献   

专业化的中国木业国际盛会——2005中国木制品进出口贸易洽谈会     

闫蕊  Kelly  Yan 《国际木业》2005,35(12):2-6

2005年10月30日至11月2日，由中国木业国际公司、中国木业国际网和加拿大R.E.Taylor ＆ Associates Ltd.共同主办的第四届《中国木制品进出口贸易洽谈会》在风景秀丽，气候宜人的广东东莞隆重举行，  相似文献   

First report on Meloidogyne chitwoodi hatching inhibition activity of essential oils and essential oils fractions     

Jorge Miguel Silva Faria  Inês Sena  Bruno Ribeiro  Ana Margarida Rodrigues  Carla Maria Nobre Maleita  Isabel Abrantes  Richard Bennett  Manuel Mota  Ana Cristina da Silva Figueiredo 《Journal of pest science》2016,89(1):207-217

The Columbia root-knot nematode (CRKN), Meloidogyne chitwoodi, is an EPPO A2 type quarantine pest since 1998. This nematode causes severe damage in economically important crops such as potato and tomato, making agricultural products unacceptable for the fresh market and food processing. Commonly used nematicidal synthetic chemicals are often environmentally unsafe. Essential oils (EOs) may constitute safer alternatives against RKN. EOs, isolated from 56 plant samples, were tested against CRKN hatching, in direct contact bioassays. Some of the most successful EOs were fractionated and the hydrocarbon molecules (HM) and oxygen-containing molecules (OCM) fractions tested separately. 24 EOs displayed very strong hatching inhibitions (≥90 %) at 2 µL mL^?1 and were further tested at lower concentrations. Dysphaniaambrosioides, Filipendula ulmaria, Ruta graveolens, Satureja montana and Thymbra capitata EOs revealed the lowest EC₅₀ values (<0.15 µL mL^?1). The main compounds of these EOs, namely 2-undecanone, ascaridol, carvacrol, isoascaridol, methyl salicylate, p-cymene and/or γ-terpinene, were putatively considered responsible for CRKN hatching inhibition. S. montana and T. capitata OCM fractions showed hatching inhibitions higher than HM fractions. The comparison of EO and corresponding fractions EC₅₀ values suggests interactions between OCM and HM fractions against CRKN hatching. These species EOs showed to be potential environmentally friendly CRKN hatching inhibitors; nonetheless, bioactivity should be considered globally, since its HM and OCM fractions may contribute, diversely, to the full anti-hatching activity.  相似文献   

Mineral nutrition and adventitious rooting in microcuttings of Eucalyptus globulus     

Schwambach J  Fadanelli C  Fett-Neto AG 《Tree physiology》2005,25(4):487-494

We characterized the adventitious rooting response of Eucalyptus globulus Labill. to various concentrations of calcium, nitrogen, phosphorus, iron, manganese, zinc, boron and copper. The parameters analyzed were percent rooting, root number, root length and mean rooting time. Root number and root length were significantly affected by mineral nutrition, whereas mean rooting time and rooting percentage seemed to be closely related to auxin availability. Root number was affected by calcium, nitrogen source and zinc, whereas root length was influenced by concentrations of phosphorus, iron and manganese, and by nitrogen source. Based on these results, we evaluated various combinations of several concentrations of these minerals in each rooting phase. Cuttings that were rooted in an optimized mineral nutrient medium and acclimatized to ex-vitro conditions for two months showed significantly higher survival after transplanting and drought stress than cuttings rooted in basal medium and treated in the same way.  相似文献   

Rheological Studies on the Reaction of Zein with Polyethylenemaleic Anhydride     

Gordon W. Selling  Jeffrey A. Byars  Kelly D. Utt 《Cereal Chemistry》2016,93(2):145-149

There continues to be interest in developing solvent‐resistant articles from biobased renewable materials to successfully compete with petrochemical products. It was previously shown that reaction of zein with polyethylenemaleic anhydride (PEMA) provides articles that are solvent resistant. The gelation kinetics for the reaction of PEMA with zein was investigated rheologically to better understand this chemistry. The reaction of the nucleophilic groups on zein with the anhydrides on PEMA is the main cause for the gelation reaction. The gelation time was defined as being the point when the elastic modulus (G′) and viscous modulus (G″) cross. In this work, the rate of reaction, in terms of time to gelation, was studied in N,N‐dimethylformamide solution for which the amount of PEMA, the reaction temperature, and the overall reaction concentration were varied. Exponential relationships were found between the gelation time and % PEMA, temperature, and % solids, as well as between elastic modulus with either % PEMA or % solids. The concentration of PEMA had the largest impact on gelation time, for which going from 2.5% PEMA to 6% PEMA reduced the gelation time from 63,114 to 1,576 s. The temperature dependence of this gelation reaction was well described by an Arrhenius plot with an apparent activation energy of 50.5 kJ/mol.  相似文献   

Indications,outcome and complications with axial pattern skin flaps in dogs and cats: 73 cases          下载免费PDF全文

E. J. Field  G. Kelly  D. Pleuvry  J. Demetriou  S. J. Baines 《The Journal of small animal practice》2015,56(12):698-706

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Reproductive and productive performances of Santa Inês ewes submitted to breeding in different periods of the Amazonian humid tropical climate     

Felipe Nogueira Soares  Maria Emilia Franco Oliveira  Luciana Cristina Padilha-Nakaghi  Luís Guilherme de Oliveira  Marcus Antônio Rossi Feliciano  Felipe Brener Bezerra de Oliveira  Pedro Paulo Maia Teixeira  Wilter Ricardo Russiano Vicente  Cristian Faturi  Luiz Fernando de Souza Rodrigues 《Tropical animal health and production》2015,47(8):1465-1471

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Hemorrhage in the central canal of the cervical spinal cord in a coonhound diagnosed with canine juvenile polyarteritis (steroid responsive meningitis-arteritis)     

Kelly L. Hughes  Susanne M. Stieger-Vanegas  Beth A. Valentine 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(6):567-570

Patchy meningeal and parenchymal contrast enhancement of the spinal cord with multifocal central canal dilations was noted in a computed tomography myelogram of the cervical spine of a 6-month-old intact female coonhound with a confirmed diagnosis of canine juvenile polyarteritis and associated hemorrhage within the central canal.  相似文献   

Plasma N‐terminal pro‐B‐type natriuretic peptide concentration in healthy retired racing Greyhounds          下载免费PDF全文

Kristen M. Couto  M. Cristina Iazbik  Liliana M. Marín  Sara Zaldivar‐López  Melissa J. Beal  P. Gómez Ochoa  C. Guillermo Couto 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2015,44(3):405-409

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