Ebstein's anomaly in a beagle dog     

Takemura N  Machida N  Nakagawa K  Amasaki H  Washizu M  Hirose H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(4):531-533

An intact male beagle dog aged 1 year was referred because of shortness of breath, exercise intolerance and cardiac murmur. Based on the results from electrocardiography, thoracic radiography and echocardiography, the dog was diagnosed as Ebstein's anomaly. Although the orally administered digoxin, vasodilators and diuretics partially improved congestive signs, the dog became to be refractory and died 20 months after the diagnosis. Necropsy confirmed malformation and apical displacement of the basal attachment of tricuspid valve leaflets.  相似文献   

Lineage classification of canine inheritable disorders using mitochondrial DNA haplotypes     

Takahasi S  Miyahara K  Ishikawa H  Ishiguro N  Suzuki M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(3):255-259

To estimate the maternal effects of dog breeds using mitochondrial DNA(mtDNA) haplotypes in the dogs with several clinical disorders, 600 base pairs of mtDNA D-loop region were amplified from 365 dogs and were determined for mtDNA sequences. The diversity of the 600-bp sequences was classified into 64 haplotypes, including 46 newly discovered haplotypes, and the haplotypes were grouped into four clusters I to IV. Lineage analysis using the mtDNA haplotype indicated that each dog breed genetically comprises one or a few mtDNA haplotypes. When the relationship between genetic background and occurrences of clinical diseases was estimated, canine lineage analysis using mtDNA haplotype revealed that the disorders distributed in the dominant mtDNA haplotypes of each dog breed, but no disorder closely associated with mtDNA haplotypes was detected.  相似文献   

Effects of LH and PGF2alpha in equine dominant follicles observed by MDS     

Kojima AY  Kobayashi S  Acosta TJ  Kudo M  Miyamoto A  Takagi M  Miyazawa K  Sato K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(2):119-122

Microdialysis System (MDS) is a novel technique used for investigation of molecule secretion between different cell populations. Local hormonal secretion at follicular wall has been still unclear. This MDS study was used to determine progesterone (P4), androstenedione (A4), estradiol-17beta (E2) and Prostaglandin F2alpha (PGF2alpha) release in mare pre-ovulatory follicles. Follicles larger than 30 mm were isolated from the ovary and follicular fluid aspirated for hormone assay. Follicular fluid collected from small, middle and large follicles were analyzed by EIA. The concentrations of P4 and PGF2alpha were similar among the different sizes of follicles. The release of A4 was observed in middle and large follicles. E2 concentration was observed in middle follicles and was higher in large follicles compared with middle follicles. Follicular wall was cut and incubated for MDS and when LH was infused, there was an increase in P4 and A4 release. PGF2alpha release was considerably high after LH infusion compared to the control group. Infusion of PGF2alpha increased P4 and A4 release but there was no change in E2 release. This results suggest that in pre-ovulatory follicles, LH stimulates theca interna cells and also PGF2alpha seemed to have a mediator role to induce steroid hormone production and luteinization of follicular cells. The nature of the mechanisms involved in selection of large follicles is still a perplexing research problem in reproduction.  相似文献   

Femtomolar bradykinin-induced relaxation of isolated bovine coronary arteries, mediated by endothelium-derived nitric oxide     

Nakamura  Kawagoe  Obi  Miyamoto  Ishiguro  & Nishio 《Journal of veterinary pharmacology and therapeutics》1998,21(4):304-308

We reported previously that bradykinin induces endothelium-dependent relaxation at nanomolar (n M ) concentrations in isolated bovine coronary arteries with an intact endothelium. Recently we have found that in the presence of 10 μ m indomethacin, femtomolar (f M ) concentrations of bradykinin induce endothelium-dependent relaxation in some bovine coronary arteries (≈ 10% of the coronary arteries examined). The present study was designed to characterize the relaxation induced by f M bradykinin. Relaxation was completely abolished by repeated application of f M bradykinin, by 100 μ m N^ω- nitro- l - arginine methyl ester and by 10 μ m methylene blue. Relaxation induced by n M bradykinin was partly affected by these treatments. Relaxation induced by both concentrations of bradykinin was inhibited by a B₂-kinin receptor antagonist, [Thi^5,8, D-Phe⁷]-bradykinin, in a concentration-dependent manner, but not by a B₁-kinin receptor antagonist, des-Arg⁹, [Leu⁸]-bradykinin. In the presence of 10 μ m captopril, an angiotensin-converting enzyme (ACE) inhibitor, all coronary arteries examined in this experiment showed endothelium-dependent relaxation to f M bradykinin. These results show that some bovine coronary arteries relax in response to f M bradykinin, and this response is mediated predominantly by the release of nitric oxide via stimulation of endothelial B₂-kinin receptors. The relaxation may be dependent on ACE activity.  相似文献   

Ratio of rice reflectance for estimating leaf blast severity with a multispectral radiometer     

Takashi Kobayashi  Eiji Kanda  Shigeo Naito  Toshihiko Nakajima  Ichiro Arakawa  Kazutoshi Nemoto  Masanao Honma  Hiroyuki Toujyou  Kiyoshi Ishiguro  Katsuki Kitada  Yoichi Torigoe 《Journal of General Plant Pathology》2003,69(1):17-22

 Rice reflectance was measured to determine the spectral regions most sensitive to leaf blast infection with a multispectral radiometer. As disease severity increased, reflectance also increased in the 400–500 nm (blue), 570–700 nm (red), and 900–2000 nm regions but decreased in the 500–570 nm and 700–900 nm regions. The increased reflectance in the blue and red regions may be attributed to decreased chlorophyll and carotenoid contents in response to the blast infection. The maximum and minimum reflectance differences occurred at 680 nm and 760 nm for the nondiseased and diseased rice, respectively. The spectral location of maximum sensitivity was 675 nm regardless of disease severity. Rice reflectance ratios were evaluated as indicators of leaf blast severity. Two ratios, R550/R675 (reflectance at 550 nm divided by reflectance at 675 nm), and R570/R675 quantified the significant disease severity. These wavelengths were selected based on the sensitivity minima and maxima. The ratios of nondiseased rice plants varied depending on growth stage. The variation in ratios must be considered when they are used to estimate leaf blast severity. Received: April 2, 2002 / Accepted: August 12, 2002  相似文献   

Leydig cell hyperplasia in an ENU-induced mutant mouse with germ cell depletion     

Khalaj M  Abbasi AR  Nishimura R  Akiyama K  Tsuji T  Noguchi J  Okuda K  Kunieda T 《The Journal of reproduction and development》2008,54(3):225-228

Repro22 is an N-ethyl-N-nitrosourea (ENU)-induced mutation in mice showing depletion of both male and female germ cells. In the present study, we investigated the male phenotypes of the mutant mouse at the adult stage. The repro22/repro22 homozygous mice showed reduced body weights as well as markedly reduced testis weights. Histological examination of the testes at 4 and 10 months of age showed no germ cells in the seminiferous tubules of the affected testis while a number of Sertoli cells were observed in the tubules. In addition to the germ cell depletion, the testes of the affected mouse contained expanded intertubular spaces that were filled by Leydig cell-like interstitial cells. These interstitial cells were confirmed to be Leydig cells by immunohistochmical staining using anti-3beta-HSD antibody. The estimated number of Leydig cells in the affected testes at 10 months of age increased approximately 2 fold compared with those of normal testes. Furthermore, the plasma testosterone levels of the affected mice at 10 months of age were significantly higher than those of the normal mice. These findings indicated that the repro22/repro22 mouse developed hyperplasia of Leydig cells that was presumably caused by the absence of germ cells in the seminiferous tubules.  相似文献   

Prototheca zopfii genotypes isolated from cow barns and bovine mastitis in Japan     

Osumi T  Kishimoto Y  Kano R  Maruyama H  Onozaki M  Makimura K  Ito T  Matsubara K  Hasegawa A 《Veterinary microbiology》2008,131(3-4):419-423

This study is the first investigation on Japanese isolates of Prototheca zopfii from bovine mastitis and the cow-barn surroundings by molecular characterization to clarify routes of infection for bovine protothecal mastitis. We performed isolation of Prototheca from cow-barn surroundings (drinking water, sewage and feces) and milk samples from cases of bovine mastitis. Genotypes of the 32 isolates of P. zopfii from cow-barn surroundings and 67 isolates from mastitis were analyzed by genotype-specific PCR assays and restriction fragment length polymorphism (RFLP) assays. All mastitis isolates were identified as P. zopfii genotype 2. Conversely, 29 isolates from cow-barn surroundings were identified as P. zopfii genotypes 1 and 3 isolates as genotype 2, respectively. Given these results, both genotypes of P. zopfii could exist in cow-barn surroundings, but no sites were identified as frequent sources of P. zopfii genotype 2. P. zopfii isolates should thus be further explored with regard to genotype to clarify the reservoir of etiological agents in bovine Prototheca mastitis.  相似文献   

Class II chitinase accumulated in the bark tissue involves with the cold hardiness of shoot stems in highbush blueberry (Vaccinium corymbosum L.)     

Takashi Kikuchi  Kiyoshi Masuda 《Scientia Horticulturae》2009

To understand the development of cold hardiness in highbush blueberry plants (Vaccinium corymbosum L.), we investigated seasonal changes in the protein compositions of current-year and overwintered shoots. Electrophoresis using SDS-polyacrylamide gels indicated that the amounts of a few proteins increased in autumn, in association with an enhancement of cold hardiness. Of these proteins, 65- and 60-kDa proteins were confirmed to be dehydrins by western blotting. While the levels of most of the accumulated proteins decreased in April, a 27-kDa protein maintained its level in the overwintered stem during spring. The amino acid sequence deduced from a cDNA for this protein showed significant similarities with known chitinases. The stem segments from overwintered shoots sampled in July showed higher cold hardiness against sub-zero temperature than those from current-year shoots, and the bark tissue of overwintered shoots had higher cold resistance than that of current-year shoots. The 27-kDa protein (BC27) was mostly found in the bark tissue of stems. Appreciable accumulation of this protein in floral buds was not detected. The purified BC27 protein exerted a cryoprotective effect on protoplasts subjected to a freeze–thaw treatment. These results suggest that the stems of highbush blueberry develop a system of cold hardiness different from that of the buds and apices, and that the accumulation of chitinase is involved in tolerance to low temperature in winter or unseasonably low temperature in spring.  相似文献   

Characterization of gelatinolytic enzymes in the skeletal muscle of red sea bream Pagrus major     

Asami Yoshida  Inwoo Bae  Hiroko Sonoda  Ryuichi Masuo  Sachie Oda  Min-Jie Cao  Kiyoshi Osatomi  Kenji Hara 《Fisheries Science》2009,75(5):1317-1322

Gelatinolytic enzymes were partially purified from the skeletal muscle of red sea bream Pagrus major and characterized to obtain information on post mortem tenderization of fish muscle. Four gelatinolytic activities, G1 (90 kDa), G2 (65 kDa), G3 (60 kDa), and G4 (100 kDa), were detected in the Q Sepharose column. G1, the major gelatinolytic enzyme, and G4 were identified as serine proteinases from results of inhibitor spectrum and substrate specificity. By contrast, G2 and G3 were found to be metalloproteinases since these were inhibited by ethylenediamine tetraacetic acid and o-phenanthroline, and activated by 4-aminophenylmercuric acetate. The optimum pH and temperature of these enzymes were in the ranges of 7–9 and 20–40°C, respectively.  相似文献   

日本对虾杆状核病毒性传染病诊断技术     

Kazuo Momoyama  李常勇 《河北渔业》1997,(2):5-10

本文提出了分别用暗视野显微技术和透射电子显微技术快速确诊日本对虾杆状核病毒性传染病的方法。在暗视野显微镜下观察到的血淋巴中的病毒粒子大小约为0.5μm;胃表层上皮被病毒感染的细胞核明显地呈白色均质小体,直径10～15μm,球形或椭圆形,坏死细胞被囊化为棕色小块(约20～50μm)。病虾血淋巴和胃经负染制样在透射电子显微镜下观察病毒颗粒大小为400×150nm;核衣壳大小为390×85nm。  相似文献