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171.
Yuko TOISHI Nobuo TSUNODA Masaaki TAGAMI Hiromitsu HASHIMOTO Fumiki KATO Tsukasa SUZUKI Kentaro NAGAOKA Gen Watanabe Shota TOKUYAMA Kiyoshi OKUDA Kazuyoshi TAYA 《Journal of Equine Science》2013,24(3):47-51
Evaluation of a new chemiluminescent enzyme immunoassay, the PATHFAST assay system
(PATHFAST), for measurement of circulating progesterone in mares was performed. Five mares
at the mid-luteal stage were administrated a single i.m. injection of prostaglandin F2α
analog (PGF2α; cloprostenol 250 μg/ml), and then blood samples were collected from the
jugular vein at 0, 15, 30 and 45 min, at one-hour intervals until 24 and at 48 hr via a
catheter in the jugular vein. To monitor the physiological changes in circulating
progesterone in mares after induced luteolysis, concentrations of progesterone in whole
blood and serum samples were measured by PATHFAST. In addition, concentrations of
progesterone in serum samples measured by PATHFAST were compared with those measured by
radioimmunoassay (RIA) and enzyme immunoassay (EIA). Using PATHFAST, the serum
concentrations of progesterone in mares correlated highly with those of whole blood
samples (r=0.9672, n=88). The serum concentrations of progesterone as measured by PATHFAST
correlated well with RIA (r=0.9654, n=88) and EIA (r=0.9323, n=112). An abrupt decline in
circulating progesterone in whole blood samples was observed within 2 hr (50%), followed
by a gradual decline until 48 hr later. The results for progesterone in whole blood
samples correlated highly with those in serum samples, and the declining pattern
paralleled that of the serum samples. These results demonstrated that PATHFAST is useful
in the equine clinic as an accurate diagnostic tool for rapid assay of progesterone within
26 min, using unextracted whole blood. 相似文献
172.
Kiyoshi Namai Yuta Matsushima Masaji Morishima Masayuki Amagai Tomohide Natsuaki 《Journal of General Plant Pathology》2013,79(6):402-411
Inoculation of tissue-cultured plants of strawberry cultivar Ichigochukanbohon-Nou2gou (Nou-2) with the anthracnose pathogen (Glomerella cingulata) results in wilting and plant death, whereas inoculation of strawberry runners grown in greenhouses results in leaf spots, not wilting or death. When tissue-cultured Nou-2 plants were acclimated for 3, 9 or 15 days, plant resistance to anthracnose increased as the acclimation period increased, suggesting that the resistance of Nou-2 may be induced by external factors. To clarify the mechanisms of resistance, we used cDNA microarray analyses to compare gene expressions among tissue-cultured and acclimated plants of the resistant cultivar Nou-2 and the susceptible cultivar Tochiotome. As a result, we identified 18 cDNA clones that were upregulated during acclimation in Nou-2 but not in Tochiotome. In a real-time RT-PCR of the 18 clones, the expression levels of three were significantly higher in acclimated plants of Nou-2. Two of the three clones showed close homology to enzymes related to flavonoid biosynthesis, i.e., leucoanthocyanidin dioxygenase (LDOX, ANS) and UDP-glucosyltransferase, putative (3-GT). The clones spotted onto the cDNA microarrays were rechecked, and 23 nonredundant cDNA clones of 13 enzymes were estimated to be flavonoid biosynthetic enzymes. Most of these clones were upregulated by acclimation in Nou-2. 相似文献
173.
Tanaka T Kassai A Ohmoto M Morito K Kashiwada Y Takaishi Y Urikura M Morishige J Satouchi K Tokumura A 《Journal of agricultural and food chemistry》2012,60(16):4156-4161
Apical application of lysophosphatidic acid (LPA), a growth-factor-like phospholipid, was shown to prevent or restore gastrointestinal (GI) disorders, such as diarrhea and stomach ulcer, in experimental animals. Because LPA is formed from phosphatidic acid (PA) by the activity of digestive phospholipase A(2), PA is a potential component for dietary treatment of such GI disorders. Here, we quantified PA contained in 38 foodstuffs and 3 herbs by a thin-layer-chromatography-imaging technique. Vegetables belonging to Brassicaceae, such as cabbage leaves (700 nmol/g of wet weight) and Japanese radish leaves (570 nmol/g), contained higher amounts of PA than other foodstuffs. Amounts of PA in fruits, cereals, and starchy root vegetables were below 300 nmol/g. Animal foodstuffs contained low amounts of PA (<60 nmol/g). Interestingly, leaves of Mallotus japonicas, a Japanese edible herb used for treatment of stomach ulcer, had the highest PA (1410 nmol/g) among those examined. The data shown here will be useful for the development of dietary treatment for a damaged GI tract. 相似文献
174.
Go Suzuki Seiji Arakaki Kiyoshi Suzuki Yukihiro Iehisa Takeshi Hayashibara 《Fisheries Science》2012,78(4):801-808
In situ larval seeding is a low-cost technique that is currently under development for the large-scale restoration of coral populations. One problem that still needs to be solved is the preparation of coral larvae for seeding, i.e., how many larvae are required to restore a certain area? In this study, we focused on the relationship between the numbers of larvae, settlers, and survivors for three?months post-settlement to determine the optimal larval seeding density. A comparison of three different larval densities (low, middle, and high) indicated that the number of settlers was proportional to the larval density, suggesting that settler density is determined by the number of larvae supplied. However, the survival rate of settlers on high-density plates was much lower than the corresponding rates on low- or middle-density plates during the first month after settlement. Moreover, most of the seeded corals had not survived on the low-density plates at three?months after settlement. Therefore, the middle larval density (i.e., 5000?larvae?m?2) appears to be optimal for seeding on grid plates. 相似文献
175.
Xiao Liang Asami Yoshida Kiyoshi Osatomi Yajun Wang Min-Jie Cao Kenji Hara 《Fisheries Science》2012,78(1):147-153
“Burnt meat” is a term used to describe the white (pale, grainy, exudative) muscle of yellowtail or tuna. It (with lightness
parameter, L* ≥ 55) was observed after 2 h storage in the suffocate in air (SA) 29°C group and after 4 h storage in the spinal cord destruction
(SCD) 29°C group. In the SA 17°C group, burnt meat was also observed after 4 h storage. In contrast, the meat in the SCD
17°C group was normal until after 12 h storage. The myosin heavy chain (MHC) was more degraded than the other myofibrillar
proteins, and some protein bands increased in the burnt meat. The protease that leads to the degradation of MHC was investigated
using myofibrils from the meat. EDTA completely suppressed the degradation, indicating that a myofibril-bound EDTA-sensitive
protease (MBESP) may exist in yellowtail muscle and this caused the degradation of MHC. The optimum pH and temperature of
MBESP in yellowtail were 5.0 and 50–60°C, respectively. 相似文献
176.
Tatsushi Kimura Tsutomu Inamizu Kiyokazu Sekikawa Masayuki Kakehashi Kiyoshi Onari 《Journal of circadian rhythms》2009,7(1):7-6
Background
Numerous processes in the living body exhibit daily rhythmicity. In this study, we characterized a daily rhythm of blood fluidity and identified its determinants. 相似文献177.
Takayoshi?Matsunaga Risa?Ieda Sho?Hosoya Miwa?Kuroyanagi Shigenori?Suzuki Hiroaki?Suetake Satoshi?Tasumi Yuzuru?Suzuki Toshiaki?Miyadai Kiyoshi?KikuchiEmail author 《Fisheries Science》2014,80(5):933-942
The tiger pufferfish (fugu) is one of the most important food fishes in East Asia. Since its testes are regarded as a delicacy, sex determination is economically relevant. Previous studies have identified a missense single-nucleotide polymorphism (SNP) in the Amhr2 (anti-Müllerian hormone receptor type II) gene as a strong candidate for a master sex-determining polymorphism. To distinguish genotypic sex efficiently, we developed a high-resolution melting (HRM) assay for this SNP site. By screening 396 fish from two independent crosses reared under controlled conditions, we observed perfect concordance between the SNP genotype and phenotypic sex. Thus, this method holds great potential for use in high-throughput sexing. When analyzing 293 progeny from a third cross reared under unknown conditions, we unexpectedly found that 25 % of phenotypic males exhibited female genotype. These results suggest that environmental factors such as rearing conditions could influence the sex-determination pathway in pufferfish. Alternatively, genetic modifiers might override the signals from Amhr2. This finding raises a concern regarding enhanced stock management of this species, because sex-reversed fish could compromise the sex ratio in subsequent generations. The HRM assay will also be useful for monitoring the degree of sex reversal before release. 相似文献
178.
Tatsuro Suzuki Toshikazu Morishita Yuji Mukasa Shigenobu Takigawa Satoshi Yokota Koji Ishiguro Takahiro Noda 《Breeding Science》2014,64(4):339-343
In a screening of about 500 lines of Tartary buckwheat, we identified lines that contained no detectable rutinosidase isozymes using an in-gel detection assay. We confirmed that seeds of these individuals had only a trace level of in-vitro rutinosidase activity. To investigate the heritability of the trace-rutinosidase characteristic, we analyzed the progeny of crosses between rutinosidase trace-lines, ‘f3g-162’, and the ‘Hokkai T8’. The F2 progeny clearly divided into two groups: those with rutinosidase activity under 1.5 nkat/g seed (trace-rutinosidase) and those with activity over 400 nkat/g seed (normal rutinosidase). The segregation pattern of this trait in F2 progeny exhibited 1 : 3 ratio (trace-rutinosidase : normal rutinosidase), suggesting that the trace-rutinosidase trait is conferred by a single recessive gene; rutinosidase-trace A (rutA). In addition, sensory panelists evaluated the bitterness of flour from trace-rutinosidase individuals and did not detect bitterness, whereas flour from normal rutinosidase individuals was found to have strong bitterness. Although at least three bitter compounds have been reported in Tartary buckwheat seeds, our present findings indicate that rutin hydrolysis is the major contributing factor to bitterness. In addition, the trace-rutinosidase line identified here, ‘f3g-162’, is a promising material for generating a non-bitter Tartary buckwheat variety. 相似文献
179.
Ado Shigihara Yuiko Matsumura Mayuko Kashiwagi Kiyoshi Matsumoto Manabu Igawa 《Journal of Forest Research》2009,14(6):394-399
The effects of simulated acid fog (SAF) and ozone (O3) stress on the growth and physiology of beech (Fagus crenata) saplings were investigated. Three-year-old beech saplings were exposed to SAFs of pH 3 and pH 5 (control) during May 2007
to July 2008. In each SAF treatment group, half of the saplings were exposed to 60 ppb of O3 during September 2007 to July 2008. In comparison to the control saplings, those from the pH 3 treatment had lower total
plant biomasses, epicuticular wax amounts, Ca2+ concentrations in their leaves, and lower starch concentrations in their leaves and roots. The effect of O3 was significant only for the starch concentration in the roots, but the O3 exposure also negatively affected the growth and physiology of beech saplings. Results show that acid fog exerts various
severe effects, and that both chronic acid fog and O3 exposure suppressed the physiological functions of beech saplings. 相似文献
180.
Noriyuki Miyake Hirofumi Nagai Shinro Kato Masashi Matsusaki Shiro Fukuta Reiko Takahashi Ryoji Suzuki Yasushi Ishiguro 《Journal of General Plant Pathology》2017,83(1):1-6
Two detection methods combining loop-mediated isothermal amplification (LAMP) and a bait trap were developed to detect Pythium helicoides in greenhouses containing roses, miniature roses, and poinsettias in hydroponic culture systems. In “Bait-LAMP”, a crude extract derived from perilla seeds as the bait was used in the LAMP reaction, whereas in the “Bait culture-LAMP”, a crude extract of mycelia grown out from perilla seeds onto Pythium-selective medium served as the bait. The two methods are simple and rapid for practical monitoring of P. helicoides in hydroponic culture systems. 相似文献