Random amplified polymorphic DNA analyses of cytoplasmic male sterile and male fertile Pigeonpea (Cajanus cajan (L.) Millsp.)     

J. Souframanien  J.G. Manjaya  T.G. Krishna  S.E. Pawar 《Euphytica》2003,129(3):293-299

Randomly amplified polymorphic DNA (RAPD) markers were used for the identification of two pigeonpea cytoplasmic genic male sterile (CMS) lines derived from crosses between the wild (Cajanus scarabaeoides & C. sericeus) and the cultivated species of Cajanus cajan. The male sterile (A) line and its maintainer (B) line could be easily differentiated with certain random primers. The two male sterile (288 A and 67 A)systems are based on C. scarabaeoidesand the other is based on C. sericeus could also be differentiated. Amplification product of 600bp amplified by primer OPC-11 was observed in both the cytoplasmic male sterile lines (288 A and 67A), which was absent in the maintainer lines (288 B and 67 B) and the putative R-line (TRR 5 and TRR 6). CMS lines, putative R lines, other cultivars and wild species under the study could be easily distinguished with the help of different primers. Dendrogram constructed based on the similarity index showed that considerable genetic variation exist sbetween CMS lines, two putative R line and wild species studied. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Species affinities between Cajanus cajan and some Atylosia species based on esterase isoenzymes     

T. G. Krishna  L. J. Reddy 《Euphytica》1982,31(3):709-713

Summary Esterase isozymes were studied in seed extracts of Cajanus cajan and six Atylosia species by polyacrylamide gel electrophoresis and isoelectrofocusing. The isozyme patterns were stable and accession specific. Within the accessions of the Atylosia species, A. albicans and A. scarabaeoides showed three common bands indicating that they are more closely related to each other than to the other species. Of the accessions of Atylosia only A. cajanifolia shares the esterase isozyme of C. cajan and hence seems to be the closest wild relative of C. cajan.  相似文献   

Initial screening of chili and sweet pepper germplasm for resistance to chili thrips, Scirtothrips dorsalis Hood     

N. K. Krishna Kumar  M. Aradya  A. A. Deshpande  N. Anand  P. R. Ramachandar 《Euphytica》1996,89(3):319-324

Summary A preliminary evaluation for resistance to chili thrips, Scirtothrips dorsalis Hood of 41 and 194 pepper (Capsicum annuum L.) germplasms during 1987 and 1988, respectively, indicated chili accessions may be a promising source of resistance. In contrast, all sweet pepper accessions tested were highly susceptible. A number of chili accessions produced a moderate yield, in spite of a high thrips infestation indicating tolerance to S. dorsalis. Rating for thrips damage was more reliable and efficient than estimating thrips numbers in screening pepper accessions for resistance to thrips. Highly significant, positive correlation between ratings at the seedling stage and final rating for thrips damage indicated the feasibility of screening pepper accessions at the seedling stage. Comparing the similarities in rating among accessions resulted in 40 distinct groups. The variance-covariance matrix of the data from these 40 groups was subjected to principal component analysis. This accounted for 56 and 18 per cent of the variation across the two principal axes, respectively. Projection of chili and sweet pepper accessions along these two axes revealed three distinct clusters. About 80 per cent of chili accessions formed the first cluster, 58 per cent of the sweet pepper accessions formed the second cluster and a third cluster exhibited intermediate ratings for thrips damage. The significance of these findings in relation to geographical divergence and resistance to thrips among pepper accessions is discussed.  相似文献   

Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids     

H. C. Prasanna  Sarvesh P. Kashyap  Ram Krishna  D. P. Sinha  Suresh Reddy  V. G. Malathi 《Euphytica》2015,204(2):407-418

  相似文献   

Book presentation     

Krishna R. Reddy 《Journal of Soils and Sediments》2003,3(1):28-28

  相似文献   

Inhibition of peroxynitrite-mediated reactions by vanillin     

Kumar SS  Priyadarsini KI  Sainis KB 《Journal of agricultural and food chemistry》2004,52(1):139-145

Several neurodegenerative diseases such as Alzeimer's and Parkinson's as well as septic shock and inflammation involve formation of reactive oxygen and nitrogen species that include peroxynitrite (PON). PON can also react with endogenous antioxidants. Therefore, dietary supplementation with antioxidants may help in these diseases. An exogenous antioxidant, vanillin (4-hydroxy-3-methoxybenzaldehyde), used widely as a food flavoring agent, was evaluated for its ability to scavenge PON and inhibit PON-mediated reactions. Nitration of tyrosine by PON was assessed by high-performance liquid chromatography (HPLC). This reaction was inhibited by vanillin. The oxidation of dihydrorhodamine 123 to fluorescent rhodamine 123 was also inhibited by vanillin. The kinetics of reaction between PON and vanillin was studied by stopped-flow technique. The products of this reaction were analyzed by HPLC, and hydroxyvanillin was identified as one of the five products with absorption at 350 nm. These data demonstrate that vanillin effectively scavenges PON in cell-free systems.  相似文献   

Diofenolan: a novel insect growth regulator in common citrus butterfly, <Emphasis Type="Italic">Papilio demoleus</Emphasis>     

Satya Singh  Krishna Kumar 《Phytoparasitica》2011,39(3):205-213

Penultimate and last instar larvae of the common citrus swallowtail butterfly, Papilio demoleus L. (Papilionidae: Lepidoptera), were treated with different doses of a novel juvenoid, diofenolan. Several deformities were observed as a result of topical administration of diofenolan which include delay in larval–larval and larval–pupal ecdysis, ecdysial failure, mortality, severe reduction in pupation, deformed pupae and complete inhibition of adult emergence. The juvenoid diofenolan severely hampers the normal growth, development and metamorphosis of P. demoleus and can be incorporated into integrated pest management programs for successful control of this important pest of citrus all over the world.  相似文献   

A qualitative assessment of nutrition knowledge levels and dietary intake of schoolchildren in Hyderabad     

Vijayapushpam T  Menon KK  Raghunatha Rao D  Maria Antony G 《Public health nutrition》2003,6(7):683-688

  相似文献   

Elevated CO2 Improves Growth and Phosphorus Utilization Efficiency in Cereal Species Under Sub-Optimal Phosphorus Supply     

Renu Pandey  Krishna Kant Dubey  Altaf Ahmad  Rakshanda Nilofar  Rachana Verma  Vanita Jain 《Journal of plant nutrition》2015,38(8):1196-1217

Cultivars of Triticum aestivum, T. durum, and Secale cereale were grown at low (2 μM) and sufficient (500 μM) phosphorus (P) under ambient carbon dioxide (380 μmol mol^?1; aCO₂) and elevated CO₂ (700 μmol mol^?1, eCO₂) to study responses of cereal species in terms of growth and P utilization efficiency (PUE) under P x CO₂ interaction. Dry matter accumulation increased under eCO₂ with sufficient P. Nevertheless, dry matter accumulated at eCO₂ with low-P was similar to that obtained at aCO₂ with sufficient P. Leaf area was 43% higher under eCO₂ with sufficient P. Significant increase in lateral root density, length and surface area were noted at low-P under eCO₂. Phosphorus use efficience (PUE) increased by 59% in response to eCO_2­ in low-P plants. Thus, eCO₂ can partly compensate effect of low-P supply because of improved utilization efficiency. Among cereals, durum wheat was more suitable in terms of PUE under high CO₂ and limiting P supply.  相似文献   

Biological Control of Late Leaf Spot of Peanut (Arachis hypogaea) with Chitinolytic Bacteria     

Kishore GK  Pande S  Podile AR 《Phytopathology》2005,95(10):1157-1165

ABSTRACT Late leaf spot (LLS), caused by Phaeoisariopsis personata, is a foliar disease of groundnut or peanut (Arachis hypogaea) with high economic and global importance. Antifungal and chitinolytic Bacillus circulans GRS 243 and Serratia marcescens GPS 5, selected among a collection of 393 peanut-associated bacteria, were applied as a prophylactic foliar spray and tested for control of LLS. Chitin-supplemented application of B. circulans GRS 243 and S. marcescens GPS 5 resulted in improved biological control of LLS disease. Supplementation of bacterial cells with 1% (wt/vol) colloidal chitin reduced lesion frequency by 60% compared with application of bacterial cells alone, in the greenhouse. Chitinsupplemented application of GRS 243 and GPS 5 also resulted in improved and stable control of LLS in a repeated field experiment and increased the pod yields by 62 and 75%, respectively, compared with the control. Chitin-supplemented application of GPS 5 was tested in six onfarm trials, and the increase in pod yields was up to 48% in kharif (rainy season). A 55-kDa chitinase was purified from the cell-free culture filtrate of GPS 5 by affinity chromatography and gel filtration. Purified chitinase of S. marcescens GPS 5 (specific activity 120 units) inhibited the in vitro germination of P. personata conidia, lysed the conidia, and effectively controlled LLS in greenhouse tests, indicating the importance of chitinolysis in biological control of LLS disease by GPS 5.  相似文献