Seasonal variation in the morphology of a bovine nasal granuloma     

De Bosschere H  Audenaerde P  Ducatelle R 《The Veterinary record》2000,146(11):322-323

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Effects of a porcine reproductive and respiratory syndrome virus infection on the development of the immune response against pseudorabies virus   总被引：5，自引：0，他引：5  

De Bruin MG  Samsom JN  Voermans JJ  van Rooij EM  De Visser YE  Bianchi AT 《Veterinary immunology and immunopathology》2000,76(1-2):125-135

The aim of this study was to investigate the effects of a porcine reproductive and respiratory syndrome virus (PRRSV) infection on the development of the immune response after pseudorabies virus (PRV) vaccination in pigs. Pigs were intranasally inoculated with the European PRRSV strain, Lelystad virus ter Huurne, and were vaccinated intramuscularly with PRV 2 weeks later (LV-PRV group). Control pigs were vaccinated with PRV only (PRV group). Eight weeks after PRV vaccination, pigs from both groups were challenged intranasally with wild-type PRV. We measured the lymphoproliferative, and the cytolytic responses to PRV of peripheral blood mononuclear cells (PBMC), isolated from blood samples. In addition, serum samples were examined for antibodies against PRV and LV. One week after PRV vaccination, PBMC proliferated abundantly to PRV in both groups. However, in the LV-PRV group the lymphoproliferative response declined after 1 week, whereas, in the PRV group, the lymphoproliferative response was high for 3 weeks and declined thereafter (P<0.05). After challenge, the lymphoproliferative response was 1 week earlier and was consistently and significantly higher in the PRV group than in the LV-PRV group. The PRV-specific killing was higher at 3 weeks after PRV vaccination and 5 weeks after PRV challenge 19+/-3 and 24+/-6%, respectively, in the PRV group, compared to 7+/-4 and 6+/-9%, respectively, in the LV-PRV group (P<0.05). However, later after vaccination and challenge the cytolytic response was identical in both groups. The antibody titre against PRV developed equally in both groups. After challenge, no PRV virus was isolated from both groups. From these results we conclude that, although PRRSV infection did cause changes in the time course of the T-lymphocyte response after PRV vaccination, PRRSV infection did not inhibit the development of vaccine-induced protection after PRV.  相似文献   

Cross-reactivity of anti-Taenia crassiceps cysticerci immune antibodies with Taenia solium antigens     

Espíndola NM  De Gaspari EN  Nakamura PM  Vaz AJ 《Veterinary parasitology》2000,89(4):321-326

A comparative study of antibody production was carried out using BALB/c mice immunized with 20 or 50microg vesicular fluid (VF)-Tcra (Taenia crassiceps) antigens, and gel of <30kD or eluate from <30kD peptides. Good IgM, IgA and IgG levels were detected by ELISA-Tcra and the antibodies presented reactivity with the <20kD peptides when tested by immunoblotting-Tcra. The antibodies from animals immunized with 20 and 50microg presented high anti-Tso cross-reactivity in ELISA (IgG>IgM and IgA). All groups presented IgG antibodies identifying the 12kD Tso-peptide.  相似文献   

Panama Disease: Cell Wall Reinforcement in Banana Roots in Response to Elicitors from Fusarium oxysporum f. sp. cubense Race Four     

De Ascensao AR  Dubery IA 《Phytopathology》2000,90(10):1173-1180

ABSTRACT The biochemical basis of tolerance in banana to Fusarium wilt, caused by the pathogen Fusarium oxysporum f. sp. cubense race four, was investigated. Tissue culture banana plants from tolerant cv. Goldfinger and susceptible cv. Williams were maintained in a hydroponic system and inoculated with conidial suspensions to evaluate the degree of tolerance to susceptibility between the two clones and to investigate the effectiveness of this technique as a potential tool for early screening for resistance in breeding programs. Similarly, defense responses were induced by treatment of the plants with an elicitor preparation from the mycelial cell walls of the pathogen. Differences in the induction of lignin and callose deposition, phenolics, and the enzymes involved in cell wall strengthening; phenylalanine ammonia lyase, cinnamyl alcohol dehydrogenase, peroxidase, and polyphenol oxidase were determined. Root tissue of the tolerant cv. Goldfinger responded to the fungal elicitor through the strong deposition of lignin, preceded by the induction or activation of the enzyme activities involved in the synthesis and polymerization thereof, whereas only slight increases were observed for the susceptible cv. Williams. No increase in callose content was observed for either clone. These results indicate an important role for cell wall strengthening due to the deposition of lignin as an inducible defense mechanism of banana roots against F. oxysporum f. sp. cubense race four.  相似文献   

以市场需求为导向学生成才为目标的教学改革     

唐德修 《四川畜牧兽医学院学报》2006,4(4):276-279

解决学校培养出的人才不是企业急需人才这个矛盾的方法。就是要以市场需求为导向，以学生成才为目标进行教学改革，确定教学体系，实行以岗位定课程的订单式教学法，企业与学校紧密结合。学校根据企业所需要的员工应具备的知识为框架组织教学，同绕思想教育体系、技能技巧体系、专业能力体系进行教学设计，对学校、教师及学生都提出了更高的要求。  相似文献   

A Urethrorectal Fistula due to Prostatic Abscess Associated with Urolithiasis in a Dog     

A Agut  X Lucas  A Castro  F De Membiela  M Soler  E Belda 《Reproduction in domestic animals》2006,41(3):247-250

An 8-year-old, entire male crossbreed dog had a 2-day history of abdominal pain and straining to urinate and defecate. A diagnosis of urolithiasis with urinary retention, and probable prostatitis was made. The dog was treated with fluids and enrofloxacin. Five days later, ultrasonography of the prostate showed two hypoechoic areas compatible with abscesses. Twelve hours later, the patient started to urinate from the rectum during micturition and urethrorectal fistula was diagnosed. The omentalization of the prostatic abscesses and castration were performed and calculi were removed. The urethrorectal fistula was treated conservatively with an indwelling urinary catheter, a low residue diet and antibiotics. The dog recovered uneventfully without recurrence of the fistula.  相似文献   

Post-thaw viability of european bison (Bison bonasus) semen frozen with extenders containing egg yolk or lipids of plant origin and examined with a heterologous in vitro fertilization assay.     

S S Pérez-Garnelo  M Oter  C Borque  C Talavera  M Delclaux  E Martínez-Nevado  A T Palasz  J De la Fuente 《Journal of zoo and wildlife medicine》2006,37(2):116-125

Basic characteristics of European bison (Bison bonasus) semen were described and the efficacies of two extenders-Triladyl, containing egg yolk, and a synthetic extender, containing soybean lipids-were tested for semen cryopreservation. Seven ejaculates were collected by electroejaculation from a 10-yr-old, European bison bull. Each ejaculate was diluted at 37 degrees C to a final concentration of 200 x 10(6) sperm/ml with Triladyl or the synthetic extender. Extended semen samples were frozen according to a standard bull semen freezing protocol. After 2 wk of storage, one straw from each extender and ejaculate was thawed, and postthaw quality was evaluated by individual sperm motility and movement rate, numbers of sperm morphologic abnormalities and intact acrosomes, functional integrity of the sperm membranes determined by hypoosmotic swelling test (HOST), viability (live-dead, eosin-nigrosin stain), and a heterologous in vitro sperm penetration assay (SPA). A total of 600 in vitro-matured bovine oocytes were inseminated with 1 X 10(6) spermatozoa of Holstein semen frozen-thawed in Triladyl (control) or of European bison semen frozen in Triladyl or the synthetic extender. Nuclear status of the oocytes was determined after 18 h of sperm-oocyte coincubation. Extender had no effect on any evaluated parameters of semen after dilution and cooling (4 hr at 5 degrees C) or in postthaw individual motility, quality of movement, and sperm morphology. However, significantly (P < 0.05) higher numbers of spermatozoa with intact acrosomes, intact membranes (HOST), and viable sperm (P < 0.01) were in semen frozen in Triladyl than in the synthetic extender. Mean values for heterologous SPA for bull (control) and for bison semen frozen in the synthetic extender were very much alike-63.3+/-10.6% and 63.1 +/- 15.9%, respectively; bison semen frozen in Triladyl was lower, 43.0+/-24.2% but not significantly different. Cumulative results from a variety of viability assays of diluted/cooled and frozen-thawed semen, including the heterologous SPA, suggest that European bison semen can be successfully frozen in both extenders tested in this study.  相似文献   

新公共管理理念与政府财务报告的完善     

王德新  吴江 《四川畜牧兽医学院学报》2006,4(1):67-69

自20世纪70年代以后，在全球掀起的一场针对公共部门治理的变革中，以绩效为导向、信息透明为保障、引进商业管理的技术与方法为主要特征的新公共管理运动成了推动这一变革的主导力。在此变革中，最基础的则是政府会计和财务报告体系的改革。论述了新公共管理的基本特征及其对政府财务报告改革提出的要求，并探讨了对我国政府会计和财务报告体系改革的相关问题。  相似文献   

国外土地整理实践对我国土地整理的启示   总被引：1，自引：0，他引：1  

丁恩俊周维禄  谢德体 《四川畜牧兽医学院学报》2006,4(2):11-15

土地整理在我国起步较晚，很多理论还不成熟。采用文献参阅法，从土地整理的发展阶段、内容和特点等方面介绍了德国、俄国和荷兰等国外土地整理的基本情况，并由此得出其对我国土地整理的启示：建立健全土地整理法律政策，重视土地整理的权属调整，拓宽土地整理融资渠道，重视土地整理中的生态环境保护，积极推动土地整理公众参与和高度重视土地整理规划等。  相似文献   

In vitro dose–response of carvacrol, thymol, eugenol and trans-cinnamaldehyde and interaction of combinations for the antimicrobial activity against the pig gut flora     

J. Michiels  J. Missotten  D. Fremaut  S. De Smet  N. Dierick 《Livestock Science》2007,109(1-3):157-160

In vitro simulations of the gastric and small intestinal fermentation with varying doses of carvacrol, thymol, eugenol and trans-cinnamaldehyde and binary combinations thereof were carried out to calculate dose–response equations for the antimicrobial effect against the main pig gut flora components. Interaction effects were evaluated following the isobole method. In gastric simulations eugenol and trans-cinnamaldehyde showed clearly less inhibitory activity towards lactobacilli than carvacrol and thymol, which could also be observed in the small intestinal simulations. The minimum concentration for carvacrol, thymol, eugenol and trans-cinnamaldehyde in small intestinal simulations to reduce the number of total anaerobic bacteria compared to control with a probability of 99.7% was 255, 258, 223 and 56 mg/l respectively. This strong activity of trans-cinnamaldehyde was due to its effect against coliform bacteria; a dose of 104 mg/l gave a reduction of 1 log CFU/ml vs. 371, 400 and 565 mg/l for carvacrol, thymol and eugenol respectively. Few combinations demonstrated synergism; most mixtures showed zero interaction or antagonism. Only carvacrol + thymol was slightly synergistic.  相似文献