A rapid technique for screening banana cultivars for resistance to Xanthomonas wilt     

Leena Tripathi  John Odipio  Jaindra Nath Tripathi  Geoffrey Tusiime 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(1):9-19

The banana Xanthomonas wilt disease (BXW) has threatened the livelihood of millions of farmers in East Africa. Use of resistant varieties is the most cost-effective method of managing this bacterial disease. A reliable and rapid screening method is needed to select resistant banana varieties. An in vitro screening method was developed for early evaluation of Xanthomonas wilt resistance using small tissue culture-grown plantlets. Eight cultivars of banana were screened with sixteen isolates of Xanthomonas campestris pv. musacearum using this method. There were significant differences (P < 0.0001) in susceptibility among the various banana cultivars tested, whereas no significant difference (P = 0.92) in pathogenicity was observed between the pathogen isolates. The cv. Pisang Awak (Kayinja) was found to be highly susceptible and Musa balbisiana resistant. Nakitembe was found to be moderately resistant while cvs Mpologoma, Mbwazirume, Sukali Ndiizi, FHIA-17 and FHIA-25 were susceptible. The susceptibility of these cultivars was further tested in vivo by artificial inoculation of potted plants with similar results. This study shows that an in vitro screening test can serve as a convenient, cheap and rapid screening technique to discriminate BXW-resistant from BXW-susceptible banana cultivars.  相似文献   

Acclimation of brackish water pearl spot (Etroplus suratensis) to various salinities: relative changes in abundance of branchial Na+/K+-ATPase and Na+/K+/2Cl− co-transporter in relation to osmoregulatory parameters     

S. Chandrasekar  T. Nich  G. Tripathi  N. P. Sahu  A. K. Pal  S. Dasgupta 《Fish physiology and biochemistry》2014,40(3):983-996

The present study was conducted to elucidate the osmoregulatory ability of the fish pearl spot (Etroplus suratensis) to know the scope of this species for aquaculture under various salinities. Juvenile pearl spot were divided into three groups and acclimated to freshwater (FW), brackish water (BW) or seawater (SW) for 15 days. The fish exhibited effective salinity tolerance under osmotic challenges. Although the plasma osmolality and Na⁺, K⁺ and Cl^? levels increased with the increasing salinities, the parameters remained within the physiological range. The muscle water contents were constant among FW-, BW- and SW-acclimated fish. Two Na+/K+-ATPase α-isoforms (NKA α) were expressed in gills during acclimation in FW, BW and SW. Abundance of one isoform was up-regulated in response to seawater acclimation, suggesting its role in ion secretion similar to NKA α1b, while expression of another isoform was simultaneously up-regulated in response to both FW and SW acclimation, suggesting the presence of isoforms switching phenomenon during acclimation to different salinities. Nevertheless, NKA enzyme activities in the gills of the SW and FW individuals were higher (p < 0.05) than in BW counterparts. Immunohistochemistry revealed that Na⁺/K⁺-ATPase immunoreactive (NKA-IR) cells were mainly distributed in the interlamellar region of the gill filaments in FW groups and in the apical portion of the filaments in BW and SW groups. The number of NKA-IR cells in the gills of the FW-acclimated fish was almost similar to that of SW individuals, which exceeded that of the BW individuals. The NKA-IR cells of BW and SW were bigger in size than their FW counterparts. Besides, the relative abundance of branchial Na⁺/K⁺/2Cl^? co-transporter showed stronger evidence in favor of involvement of this protein in hypo-osmoregulation, requiring ion secretion by the chloride cells. To the best of our knowledge, this is the first study reporting the wide salinity tolerance of E. suratensis involving differential activation of ion transporters and thereby suggesting its potential as candidate for fish farming under different external salinities.  相似文献   

Release of Carbon in Different Molecule Size Fractions from Decomposing Boreal Mor and Peat as Affected by Enchytraeid Worms     

Mari Lappalainen  Marjo Palviainen  Jussi V.K. Kukkonen  Heikki Setälä  Sirpa Piirainen  Tytti Sarjala  Harri Koivusalo  Leena Finér  Samuli Launiainen  Ari Laurén 《Water, air, and soil pollution》2018,229(7):240

Terrestrial export of dissolved organic carbon (DOC) to watercourses has increased in boreal zone. Effect of decomposing material and soil food webs on the release rate and quality of DOC are poorly known. We quantified carbon (C) release in CO₂, and DOC in different molecular weights from the most common organic soils in boreal zone; and explored the effect of soil type and enchytraeid worms on the release rates. Two types of mor and four types of peat were incubated in laboratory with and without enchytraeid worms for 154 days at +?15 °C. Carbon was mostly released as CO₂; DOC contributed to 2–9% of C release. The share of DOC was higher in peat than in mor. The release rate of CO₂ was three times higher in mor than in highly decomposed peat. Enchytraeids enhanced the release of CO₂ by 31–43% and of DOC by 46–77% in mor. High molecular weight fraction dominated the DOC release. Upscaling the laboratory results into catchment level allowed us to conclude that peatlands are the main source of DOC, low molecular weight DOC originates close to watercourse, and that enchytraeids substantially influence DOC leaching to watercourse and ultimately to aquatic CO₂ emissions.  相似文献   

Prevalence of house dust mites in the homes of atopic dogs in Finland          下载免费PDF全文

Kirsti J. M. Schildt  Riitta T. K. Seppänen  Anna K. Hielm‐Björkman  Leena E. Saijonmaa‐Koulumies  Svetlana Belova 《Veterinary dermatology》2017,28(2):225-e54

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Response of transplanted rice to nitrogen fertilization under different weed management practices     

H. L. Sharma  C. M. Singh  B. Tripathi 《国际虫害防治杂志》2013,59(2):108-110

Abstract

Rice yield increased significantly and consistently up to 120 kg N/ha when weeds were not controlled. But when weeds were effectively controlled by two hand weedings (20 and 40 days after transplanting) or by use of thiobencarb at 1–0 kg a.i./ha rice yield increased significantly up to 80 kg N/ha. The optimum dose of N for transplanted rice under mid‐hill conditions of Himachal Pradesh (Palampur) was 82 kg/ha when two hand weedings were done and 86 kg/ha when weeds were managed using thiobencarb. The yield response per kg of N in the respective treatments was 20 and 24 kg of grain. The weed dry weight was significantly lower in thiobencarb and hand weeded plots compared with unweeded check though the difference in weed dry weight due to the former two treatments was not significant.  相似文献   

Insect pests of stored ergot sclerotia†     

D. Singh  A. K. Tripathi 《国际虫害防治杂志》2013,59(4)

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Numerical Modelling of Waste Stabilization Ponds: Where Do We Stand?     

Leena Sah  Diederik P. L. Rousseau  Christine M. Hooijmans 《Water, air, and soil pollution》2012,223(6):3155-3171

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Allergenicity assessment of the papaya ringspot virus coat protein expressed in transgenic rainbow papaya     

Fermín G  Keith RC  Suzuki JY  Ferreira SA  Gaskill DA  Pitz KY  Manshardt RM  Gonsalves D  Tripathi S 《Journal of agricultural and food chemistry》2011,59(18):10006-10012

The virus-resistant, transgenic commercial papaya Rainbow and SunUp (Carica papaya L.) have been consumed locally in Hawaii and elsewhere in the mainland United States and Canada since their release to planters in Hawaii in 1998. These papaya are derived from transgenic papaya line 55-1 and carry the coat protein (CP) gene of papaya ringspot virus (PRSV). The PRSV CP was evaluated for potential allergenicity, an important component in assessing the safety of food derived from transgenic plants. The transgene PRSV CP sequence of Rainbow papaya did not exhibit greater than 35% amino acid sequence homology to known allergens, nor did it have a stretch of eight amino acids found in known allergens which are known common bioinformatic methods used for assessing similarity to allergen proteins. PRSV CP was also tested for stability in simulated gastric fluid and simulated intestinal fluid and under various heat treatments. The results showed that PRSV CP was degraded under conditions for which allergenic proteins relative to nonallergens are purported to be stable. The potential human intake of transgene-derived PRSV CP was assessed by measuring CP levels in Rainbow and SunUp along with estimating the fruit consumption rates and was compared to potential intake estimates of PRSV CP from naturally infected nontransgenic papaya. Following accepted allergenicity assessment criteria, our results show that the transgene-derived PRSV CP does not pose a risk of food allergy.  相似文献   

Agro-morphological variation in “Jhinuwa” rice landraces (Oryza sativa L.) of Nepal     

Mahendra P. Tripathi  Bhuwon R. Sthapit  Lakshmi P. Subedi  Srawan K. Sah  Sanjaya Gyawali 《Genetic Resources and Crop Evolution》2013,60(8):2261-2271

Jhinuwa is an aromatic rice (Oryza sativa L.) landrace from the Pokhara Valley of Nepal. A total of 210 accessions comprising seven types of Jhinuwa rice landraces were randomly collected from the rice fields to evaluate inter- and intra-population variability based on agro-morphological traits. The experiment was conducted in factorial randomized complete block design with three replicates in 2005. The first six principal components (PCs) accounted for 76.6 % variation for agro-morphological traits. Major traits that accounted for the variation by six PCs includes days to heading, days to maturity, total grain panicle^?1, fertile grain panicle^?1, culm length, panicle length, milling recovery, head rice recovery, aroma, 1,000 grain weight, sterile grain panicle^?1, grain sterility %, and leaf characteristics. Both principal coordinate analysis and cluster analyses revealed four phenotypic groups, two of which represent Bayarni, Jhinuwa, and Biramphul while the other two account for Tunde and Pakho Tunde. Tunde, Pakho Tunde, Kalo Bayarni, and Seto Bayarni showed higher intra- as well as inter-population variation compared to other populations. The phenotypic and genotypic coefficients of variation, broad sense heritability (h ²B) and genetic advance (GA) as a percent of the mean assessed for 210 accessions revealed high h ²B and GA estimates for leaf width, leaf length breadth ratio, ligule length, sterile grain panicle^?1, grain sterility % and 1,000 grain weight. The current study demonstrates that improvement in Jhinuwa rice landrace is possible by selecting superior accessions from existing natural populations while selection should be focussed to market traits with higher h ²B and GA estimates.  相似文献   

Enhanced production of dihaploid lines via anther culture of tetraploid potato (Solanum tuberosum L. ssp.tuberosum) clones     

Veli-Matti Rokka  Leena Pietilä  Eija Pehu 《American Journal of Potato Research》1996,73(1):1-12

A total of 1000 anther-derived plants was regenerated from tetraploid potato (Solanum tuberosum L.) genotypes. Capacity to undergo androgenesis was analysed in 41 potato cultivars and 7 clones grown either in the greenhouse or in the field. Of the 48 genotypes, 33 produced embryos and 23 regenerated shoots from embryos. Anther-derived plantlet production was determined in genotypes 86110, Agria, Calgary, Escort, Helios, Idole, JO 0982 JO 1432, Kainuun Musta, Kardal, KE48, Matilda, Nicola, Petra, Pito, Rustica, Stirling, Torridon, Ute, Van Gogh, Vebeca, Vento and White Lady. The highest number of shoots (24 shoots/100 anthers) was obtained from cv. Calgary, when anthers were isolated from field-grown donor plants. Incubating anthers at 28 C, rather than at 20 C or 24 C, enhanced embryo production in four genotypes tested. However, shoot production was improved only in cv. Pito cultured at 28 C. When anthers of cv. Petra were cultured at 28 C for four weeks, followed by reduction of culture temperature to 24 C, a high rate of shoot production was recorded (14 shoots/100 anthers). The ratio between dihaploids and tetraploids varied among the anther-derived plants of the different genotypes. The number of dihaploids was highest in potato clone JO1432 (100%) and in cv. Calgary (93%) and lowest in cvs. Pito (21%) and Torridon (6%).  相似文献