Canine cutaneous melanocytic tumours: significance of β‐catenin and survivin immunohistochemical expression          下载免费PDF全文

Laura Bongiovanni  Alessandra D'Andrea  Ilaria Porcellato  Andrea Ciccarelli  Daniela Malatesta  Mariarita Romanucci  Leonardo Della Salda  Luca Mechelli  Chiara Brachelente 《Veterinary dermatology》2015,26(4):270-e59

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Cloning and characterization of gene-resistant analogs (RGAs) involved in rust (Puccinia psidii) resistance in Eucalyptus grandis     

Marcelo Luiz Laia  Acelino Couto Alfenas  Sergio Hermnio Brommonschenkel  Shinitiro Oda  Eduardo Jose de Melo  Inae Marie de Arau jo Silva  Janana Fernandes Goncalves  Ariadne Marques 《林业研究》2015,(3)

Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. Most of the disease-resistant genes encode pro-teins with conserved leucine rich r...  相似文献   

Permeability profile estimation of flavonoids and other phenolic compounds by biopartitioning micellar capillary chromatography     

Diniz A  Escuder-Gilabert L  Lopes NP  Gobbo-Neto L  Villanueva-Camañas RM  Sagrado S  Medina-Hernández MJ 《Journal of agricultural and food chemistry》2007,55(21):8372-8379

This paper points out the usefulness of biopartitioning micellar chromatography (BMC) using capillary columns as a high-throughput primary screening tool providing key information about the oral absorption, skin permeability, and brain-blood distribution coefficients of 15 polyphenols (6 flavones, 2 flavonols, a flavanone, 2 flavan-3-ols, 3 phenolic acids, and a phloroglucinol) in a simple and economical way. For the compounds studied, except vicenin-2, rutin, chlorogenic acid, p-hydroxycinnamic acid, and 4-hydroxybenzoic acid, maximal oral absorption (>90%) can be expected, if there are not solubility problems or metabolic processes. On the other hand, the most retained compounds in BMC, that is, 5-hydroxyflavone, flavone, and flavanone, show the highest brain-blood distribution coefficients and skin permeability coefficients.  相似文献   

Influence of phosphorus fertilization on the response of pinus genotypes to glyphosate subdoses     

de Andrade  Thiago Cavalcante Gomes Ribeiro  Bacha  Allan Lopes  de Camargo  Mariane Bueno  de Carvalho  Leonardo Bianco 《New Forests》2022,53(1):143-160

New Forests - Previous studies have reported that phosphorus transporters are involved in glyphosate uptake by plants, so phosphorus fertilization might act as an attenuator of glyphosate effects...  相似文献   

Litter production and nutrient deposition from native woody species in the Brazilian semi-arid region     

Primo  Anacláudia Alves  Araújo  Maria Diana Melo  Silva  Karla da Fonseca  Silva  Ludmyla Araújo  Pereira  Graziella de Andrade Carvalho  Fernandes  Francisco Éden Paiva  Pompeu  Roberto Cláudio Fernandes Franco  Natale  William  de Souza  Henrique Antunes 《Agroforestry Systems》2021,95(8):1459-1464

Agroforestry Systems - The use of native trees in agroecosystems is a promising way to increase litter deposition and nutrient cycling and foster the recovery of degraded areas, especially in...  相似文献   

Short-wave solar radiation level willingly tolerated by lactating Holstein cows in an equatorial semi-arid environment     

Steffan Edward Octávio Oliveira  Cíntia Carol de Melo Costa  João Batista Freire de Souza Jr  João Paulo Araújo Fernandes de Queiroz  Alex Sandro Campos Maia  Leonardo Lelis de Macedo Costa 《Tropical animal health and production》2014,46(8):1413-1417

The amount of short-wave solar radiation willingly tolerated by lactating Holstein cows on pasture was determined in an equatorial semi-arid environment. The study was carried out on a dairy farm located in Limoeiro do Norte, CE, northeastern Brazil. The observed behaviours were as follows: grazing, under the sun, under the shade, standing, lying, ruminating, idling and wallowing in the water. The behaviours were recorded using instantaneous scan sampling at regular intervals of 15 min from 0600 to 1800 hours over 5 days. On all sampling days, the meteorological variables, including local short-wave solar radiation (R _S-W, W m^?2), were recorded. The R _S-W data were divided into five levels. The sun exposure was more frequent under low (100 %) and moderately low (97 %) levels, when R _S-W remained below 500 W m^?2. The grazing was more intense under low (100 %) and moderately low (93 %) levels. Above 500 W m^?2, the grazing time significantly decreased (11 %). The cows avoided grazing under high (0 %) and very high (0 %) levels, when R _S-W exceeded 700 W m^?2. The ruminating behaviour was more frequent under high (33 %) and very high (37 %) levels, in which the highest averages of R _S-W were recorded (815 and 958 W m^?2, respectively). The standing posture was more frequent under low (100 %) and moderately low (97 %) levels. Therefore, the critical R _S-W level that motivates cows to stop grazing and seek shade was in the interval between 500 and 700 W m^?2.  相似文献   

Higher feeding diets effects on age and liveweight gain at puberty in crossbred Nelore × Hereford heifers   总被引：1，自引：1，他引：0  

Júlio Otávio Jardim Barcellos  Gabriel Ribas Pereira  Eduardo Antunes Dias  Concepta McManus  Leonardo Canellas  Mari Lourdes Bernardi  Adriana Tarouco  Ênio Rosa Prates 《Tropical animal health and production》2014,46(6):953-960

This study was conducted to determine the age and liveweight at puberty of 120 crossbred beef heifers submitted to four diets to achieve predetermined weight gains (kg/day): 0.5 (G500; n?=?32), 0.75 (G750; n?=?32), 1.0 (G1000; n?=?29), and 1.25 (G1250; n?=?27). Animals were classified depending on their level of crossing between Nelore (N) and Hereford (H): 25%N-75%H, 37.5%N-63.5%H, 43.7%N-56.7%H, 50%N-50%H, and 75%N-25%H. Reproductive evaluation was performed at the beginning and at the end of the experimental period by ultrasonography and hormone analysis. The average age and liveweight at puberty were 388.0?±?1.9 days and 331.4?±?1.3 kg, respectively. Animals from the 25%N-75%H group reached puberty earlier than heifers from other genetic groups supplemented with G1250 diet (P?<?0.05). Heifers with higher degree of Nelore (75%N-25%H) fed with G1000 diet showed estrus 42 days prior to mating, but only 57 % reached puberty at mating (P?<?0.05). Heifers with follicles of higher diameter reached puberty (P?<?0.05) earlier. Higher average daily weight gain showed a positive effect on follicular diameter and IGF-I level at puberty (P?<?0.01). Concentrations of GH were lower in heifers fed G1250 compared to G1000 diet (P?<?0.05). There was a significant interaction between nutritional level and insulin levels at puberty (P?<?0.01). We demonstrated the relationship between IGF-I and average daily gain on the onset of puberty in heifers. In conclusion, heifers submitted to the higher feeding level showed a higher follicular diameter and were younger at puberty.  相似文献   

First report of Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) in pig herds of Brazil     

Daiane Güllich Donin  Raquel de Arruda Leme  Alice Fernandes Alfieri  Geraldo Camilo Alberton  Amauri Alcindo Alfieri 《Tropical animal health and production》2014,46(3):523-528

Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) have been associated with enteric, respiratory, reproductive and neurological disorders. Although Brazil is the world’s fourth largest producer and exporter of pork, no information on the occurrence of PTV, PSV and EV-G infections is available for Brazilian pig herds. This study aimed to investigate the occurrence of Porcine enteric picornavirus infections in pig farms located in three distinct geographical regions of Brazil. Forty randomly selected diarrhoeic and normal consistency faeces of suckling (n?=?22) and nursery (n?=?18) pigs from farms located in 21 distinct cities of the Southern, Southeast, and Midwest regions of Brazil were evaluated by nested-RT-PCR assays. Suckling piglets presented the expected amplicon size for PTV (158 bp) and EV-G (313 bp) in single and mixed infections in 40.9 % (9/22) of the faecal samples. PSV amplicon (212 bp) was not detected in this age group. For nursery pigs, Porcine enteric picornaviruses amplicons were present in 77.8 % (14/18) of the faecal samples. PTV and EV-G were detected in single and mixed infections, while PSV was detected only in two samples in co-infection with PTV and EV-G in this age group. The Brazilian regions evaluated presented at least two of the tested viruses. Sequencing analysis revealed high similarities to the related viruses (95.3 to 99.2 % for PTV, 94.2 to 98.5 % for PSV and 86 to 100 % for EV-G). For the first time PTV, PSV and EV-G have been molecularly detected and characterised in pig faecal samples in Brazil.  相似文献   

Identification and quantification of benzimidazole resistance polymorphisms in Haemonchus contortus isolated in Northeastern Brazil     

Jessica Maria Leite dos Santos  Jomar Patrício Monteiro  Wesley Lyeverton Correia Ribeiro  Iara Tersia Freitas Macedo  Ana Lourdes Fernandes Camurça-Vasconcelos  Luiz da Silva Vieira  Claudia Maria Leal Bevilaqua 《Veterinary parasitology》2014,199(3-4):160-164

Haemonchus contortus is the most prevalent nematode in Brazil. The objective of this study was to select 6 populations of H. contortus of known or suspected benzimidazole resistance status and characterize these using quantitative real-time polymerase chain reaction (qPCR) for single nucleotide polymorphisms (SNPs) F200Y, F167Y and E198A in the β-tubulin isotype 1 gene. qPCR was performed using DNA from a pool of 10 adult male H. contortus from a single animal per farm. Faecal egg count reduction test (FECRT) and egg hatch test (EHT) were used to determine the resistance status. Samples were obtained from 6 farms located in 5 counties in the Ceará State: Tauá, Boa Viagem, Quixadá, Santa Quitéria and Solonópole. The inbred-susceptible-Edinburgh (ISE) isolate was used as reference for comparative purposes in the qPCR. Benzimidazole resistance was detected by FECRT on all farms with efficacy values ranging from 0 to 51%. EC50 values as determined by EHT were all above 1.49 μg/ml. High frequencies of the resistant SNPs F200Y and F167Y alleles were detected but no resistance was detected at SNP E198A. Our results suggest that the SNPs F167Y and F200Y are both important for benzimidazole resistance in the studied populations.  相似文献   

Use of Thermography Techniques in Equines: Principles and Applications     

Veronica Redaelli  Domenico Bergero  Enrica Zucca  Francesco Ferrucci  Leonardo Nanni Costa  Lorenzo Crosta  Fabio Luzi 《Journal of Equine Veterinary Science》2014

This review of the use of thermographic technique in equines introduces the principles upon which infrared radiation and thermoregulatory physiology are based and describes the instrumentation used and its practical use. The advantage of this imaging technique is that it is a noninvasive thermographic examination, both from an operational (the animal and the operator) and health (no penetrating radiation is used) standpoint. Advantages and disadvantages of this technique, equine applications, and physiological assessments are discussed.  相似文献