Prevalence of antimicrobial resistance in enteric Escherichia coli from domestic pets and assessment of associated risk markers using a generalized linear mixed model     

Liliana R. Leite-Martins  Maria I.M. Mahú  Ana L. Costa  Ângelo Mendes  Elisabete Lopes  Denisa M.V. Mendonça  João J.R. Niza-Ribeiro  Augusto J.F. de Matos  Paulo Martins da Costa 《Preventive veterinary medicine》2014

Antimicrobial resistance (AMR) is a growing global public health problem, which is caused by the use of antimicrobials in both human and animal medical practice. The objectives of the present cross-sectional study were as follows: (1) to determine the prevalence of resistance in Escherichia coli isolated from the feces of pets from the Porto region of Portugal against 19 antimicrobial agents and (2) to assess the individual, clinical and environmental characteristics associated with each pet as risk markers for the AMR of the E. coli isolates.  相似文献   

A male-fertile Lycopersicon esculentum × Solanum lycopersicoides hybrid enables direct backcrossing to tomato at the diploid level     

Roger T. Chetelat  Pedro Cisneros  Liliana Stamova  Charles M. Rick 《Euphytica》1997,95(1):99-108

Solanum lycopersicoides Dun. is a wild nightshade native to Chile and Peru that possesses many traits of potential interest to tomato breeders, including environmental stress tolerance, resistances to disease and insect pests, and certain fruit quality characteristics. Sexual and somatic hybrids with the cultivated tomato (Lycopersicon esculentum Mill.) are readily obtained, yet have a strong tendency towards male-sterility combined with unilateral incompatibility, barriers that have deterred breeding efforts in the past. We report herein the synthesis of a partially male-fertile F₁ hybrid by sexual crosses between tomato and a recently collected population of S. lycopersicoides. Over 280 BC₁ plants were obtained by directed backcrossing to tomato at the diploid level, of which 58 were sufficiently fertile to permit selfing or additional backcrosses. The transmission of S. lycopersicoides genetic markers into a uniform L. esculentum background was confirmed with 24 isozyme or morphological loci on 11 of the 12 alien chromosomes. Potentially useful tolerance to gray mold (Botrytis cinerea) was demonstrated by inoculation of stem cuttings with mycelial plugs: at 6 days post-inoculation, the intergeneric hybrid showed little evidence of disease progression and the length of stem lesions averaged only one third that of susceptible controls. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Multi-input RNAi-based logic circuit for identification of specific cancer cells     

Xie Z  Wroblewska L  Prochazka L  Weiss R  Benenson Y 《Science (New York, N.Y.)》2011,333(6047):1307-1311

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Pericarp total protein profiles as molecular markers of tomato fruit quality traits in two segregating populations     

Gustavo R. Rodríguez  Javier H. Pereira da Costa  Damian D. Tomat  Guillermo R. Pratta  Roxana Zorzoli  Liliana A. Picardi 《Scientia Horticulturae》2011

The tomato fruit quality results of biochemical and physiological changes that occur during the ripening process. Although, the pericarp total protein profiles are less polymorphic than DNA-based markers the polymorphism in those could be directly associated with fruit quality traits. The aim of this work was to identify associations between polymorphic polypeptides from fruit pericarp at two ripening stages and fruit quality traits evaluated in two segregating populations of tomato. A cross between a normal ripening cultivar of Solanum lycopersicum (C, Caimanta) and a genotype carrying the nor (non ripening) gene (N) as well as a cross between Caimanta and a cherry type tomato of S. lycopersicum var. cerasiforme (Ce) showed genetic variance for several fruit quality traits such as fruit weight, shape, solids soluble content, acidity, color and fruit shelf life. The quantitative variations observed at phenotypic level had correspondence with the polymorphism detected in the protein profiles. Indeed, the polymophic polypeptides associated with quality fruit traits and fruit shelf life would be useful to assist tomato breeding programs as protein molecular markers.  相似文献   

Usefulness of Stress Indicators in Forecasting Population Trends in Microtus arvalis (Pallas)1     

M. Hamar  Al. Tuta  Anca Petrescu  Liliana Babes 《EPPO Bulletin》1977,7(2):349-357

The appearance of a stress condition in Microtus arvalis (Pallas) populations was established on the basis of histochemical analyses and correlated with population dynamics. It was found that stress appears only during invasions, i.e. when crop damage has already occurred. Commonly, the population crash occurs 30 to 50 days after the appearance of stress. It is concluded that stress indices can neither be used for warnings nor for long-term forecasts of outbreaks.  相似文献   

Dermal microdialysis in the dog: in vivo assessment of the effect of cyclosporin A on cutaneous histamine and prostaglandin D2 release     

Brazís P  Barandica L  García F  Clough GF  Church MK  Puigdemont A 《Veterinary dermatology》2006,17(3):169-174

Dermal microdialysis, a relatively noninvasive technique, allows investigation of the changes in cellular mediators released during cutaneous allergic responses. This technique was used to evaluate the effect of cyclosporin A, an immunosuppressive drug used for treatment of canine atopic dermatitis, on the cutaneous release of two pro-inflammatory mediators following intradermal allergen challenge. Four beagle dogs spontaneously sensitized to Ascaris suum were treated for 1 month with oral cyclosporin A. At days 0, 15 and 30 of the treatment, dialysis probes were inserted into the skin of the back, and 20 microL of A. suum antigen was injected intradermally at each site. At timed intervals, dialysate was collected and assayed for histamine and prostaglandin D(2) and the wheal area was measured. Mean histamine concentration and wheal area were significantly lower at days 15 and 30 of treatment, compared with day 0. However, prostaglandin D(2) concentration was not significantly reduced. The inhibition in histamine release after intradermal challenge, by cyclosporin, confirms its anti-inflammatory action in the dog. Dermal microdialysis provides a useful tool for investigating canine allergic reactions and their modulation by drugs.  相似文献   

Experimental evaluation of ophthalmic devices and solutions using rabbit models     

Werner L  Chew J  Mamalis N 《Veterinary ophthalmology》2006,9(5):281-291

OBJECTIVE: To analyze and compare the geometry of the anterior segment of rabbit and human eyes, with relevance for the evaluation of intraocular lenses, and to review rabbit models used in our laboratory for the evaluation of different ophthalmic devices and solutions. PROCEDURES: Fifteen rabbit and 15 human eyes (10 phakic and 5 pseudophakic/group) obtained postmortem were used. Anterior-posterior length, equatorial diameter, and white-to-white (corneal diameter) were measured with calipers. The eyes were then analyzed with a very high-frequency ultrasound (Artemis, Ultralink) for measurements of the anterior chamber depth, and anterior chamber and ciliary sulcus diameters. The capsular bag diameter was measured with calipers from a posterior view, and the diameter and thickness of the crystalline lenses were measured after their excision from the phakic eyes. RESULTS: Although the size of the rabbit eye is overall smaller than the size of the human eye, the dimensions of the anterior segment of rabbit eyes are generally larger. The differences between rabbit and human eyes were statistically significant (Wilcoxon rank sum test) in terms of anterior-posterior length, equatorial diameter, white-to-white measurements (P < 0.0001), anterior chamber diameter (P = 0.0004), ciliary sulcus diameter (P = 0.0012), and crystalline lens diameter and thickness (P = 0.0003). CONCLUSIONS: Experimental evaluation of design features of new phakic intraocular lenses in rabbit eyes may be inconclusive without adaptation of their size/design, contrary to the evaluation of new pseudophakic lenses by implantation in the capsular bag. The rabbit is a very valuable model for the experimental evaluation of different ophthalmic devices and solutions.  相似文献   

Seasonal changes in feed intake,diet digestibility and diet composition by lactating and non‐lactating goats browsing in a semi‐arid rangeland of Argentina     

ngela V. Egea  María L. Bakker  Liliana I. Allegretti  Sebastin A. Paez  Diego J. Grilli  Juan C. Guevara  Juan J. Villalba 《Grass and Forage Science》2019,74(1):115-128

We determined the seasonal nutrient and secondary compound intake, in vivo diet digestibility (DD) and diet species composition of goats in different physiological states in a desert rangeland of Argentina. During the wet and the dry seasons, 24 mature female Creole goats (six non‐lactating, non‐pregnant and six lactating in each season) were used in a 2 × 2 factorial design. Diet botanical composition, DD and dry‐matter intake (DMI) were estimated by the combined use of visual observation, microhistological analyses of faecal samples and n‐alkane technique. Browse species dominated the goats’ diets (>80%) in both seasons and physiological states, with a twofold increase in the proportion of forbs during the wet season (p < 0.10). Lactating goats showed greater DMI than non‐lactating animals, and they also displayed greater intakes of some tanniferous (Mimosa ephedroides) and high‐protein (Atriplex lampa) shrubs (p < 0.10). Lactating and non‐lactating goats selected diets with adequate crude protein (CP) and fibre content but insufficient energy, although lactating goats tended to increase energy and tannin content in their diets (p < 0.20). Physiological state did not influence DD, but digestibility was greater (p < 0.10) in the wet season, attributed to the greater protein content and lower tannin content of the diet. These results have important implications for managerial interventions on traditional goat production systems in the study area aiming at enhancing goat nutrition and welfare, as well as the sustainability of rangeland resources in the region.  相似文献   

Response of an agricultural soil to pentachlorophenol (PCP) contamination and the addition of compost or dissolved organic matter   总被引：1，自引：0，他引：1  

Rosalia Scelza  Maria Antonietta Rao  Liliana Gianfreda   《Soil biology & biochemistry》2008,40(9):2162-2169

The response of a fresh, agricultural soil when contaminated with pentachlorophenol (PCP) and supplemented with compost (C) or dissolved organic matter (DOM) was studied in the laboratory. The concentration of PCP and the changes in various functionally related properties (i.e. microbial biomass, basal respiration, soil hydrolase and oxidoreductase activity) were measured over 150 d. Variations in the main physical and chemical properties of the soils were also monitored. Two different doses of compost (C1 = 0.27% and C2 = 0.83%, corresponding to 10 and 30 t ha⁻¹, respectively) or DOM (D1 = 0.07% and D2 = 0.2%) equivalent to the carbon content of the two compost doses C1 and C2 were used and the following five systems were investigated: soil (S), soil–compost (S-C1 and S-C2) and soil–DOM (S-D1 and S-D2). PCP concentrations declined progressively and significantly with time. This effect was most pronounced for the soils amended with the lower compost dose C1 (S-C1) and with the two DOM (S-D1 and S-D2) amounts. Significantly reduced amounts of PCP were extracted after its 500-d residence in the various systems. Higher amounts of the residual PCP were extracted from the humic acids (HA), fulvic acids (FA) and humin–mineral (HU) fractions of the 500 d aged samples than from the same unfractionated samples, indicating that the residual PCP preferentially accumulated in the organic fractions of soil. The soil showed an endogenous microbial activity as indicated by basal respiration, microbial biomass and all the enzymatic activities tested (dehydrogenase, glucosidase, phosphatase, arylsulphatase and urease). Addition of the PCP severely depressed some of the tested biochemical properties suggesting an inhibitory effect on microbial activity. Conversely, higher basal respiration, and similar β-glucosidase and phosphatase activities were measured in comparison with the controls. No significant effects were observed following the addition of two doses of the compost or the DOM. Fungal colonies belonging to the taxonomic group of Ascomycetes and identified as Byssochlamys fulva developed with time in all the PCP-contaminated samples. Growth of B. fulva in vitro in the presence of PCP showed that the isolate was tolerant to 12.5 and 25 mg l⁻¹ PCP and degraded 20% of its initial concentration in 8 d. Overall, the results indicate that many complex processes occurred in the contaminated soil and combinations of these determined the response to PCP contamination. The sorption of PCP to the soil matrix (which increased with time) and its degradation/transformation by indigenous soil microbial activity were likely involved. Both the processes appeared to be favoured by the presence of dissolved organic matter.  相似文献   

Characterisation of resistance to potato leafroll virus accumulation in Solanum phureja     

Liliana Franco-Lara  Hugh Barker 《Euphytica》1999,108(2):137-144

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