Leukotrienes Affect Secretory Function of Ovarian Cells In Vitro*     

AJ Korzekwa  TJ Acosta  M Miklewicz  K Okuda  SH Lee  DJ Skarzynski 《Reproduction in domestic animals》2010,45(6):e288-e296

The aim of this study was to determine which cells are the source of production and target for leukotriene (LTs) action within the bovine ovary. Luteal (CL, days 14–16 of the oestrous cycle), steroidogenic cells (LSC) and endothelial cells (LEC) of the bovine corpus luteum (CL), and granulosa cells (GC) were isolated enzymatically, cultured in a monolayer and incubated with LTC₄, LTB₄, Azelastine (an antagonist of LTC₄) or Dapsone (an antagonist of LTB₄). Then cells were collected for determination of mRNA expression for LT receptors (LTRs) and 5‐lipoxygenase (5‐LO) by real time RT‐PCR, and media were collected for determination of prostaglandin (PG)E₂, F_2α, progesterone (P4; LSC only), endothelin‐1 (ET‐1; LEC only) and 17‐β oestradiol (E2; GC only). The greatest mRNA expression for LTR‐II and 5‐LO were found in LEC, whereas LTR‐I mRNA expression did not differ among cell types. The level of PGE₂ increased after LTs treatment in each type of ovarian cell, excluding LTC₄ treatment in LEC. The secretion of PGF_2α was also increased by LTs, but decreased after LTB₄ treatment of LSC. In GC cultures, both LTs stimulated E2 secretion; in LEC cultures, LTB₄ stimulated whereas LTC₄ inhibited P4 secretion; in LEC cultures, LTC₄ stimulated but LTB₄ inhibited ET‐1 secretion. The results show that LTs are produced locally and are involved in PGs production/secretion in all examined cells (LSC, LEC and GC) of bovine ovary. Leukotriene treatment modulate secretion of E2, by GC, P4 by LSC and ET‐1 by LEC, which indicates that LTs are involved in regulation of ovarian secretory functions.  相似文献   

Otic pathology of caprine beta-mannosidosis     

J A Render  K L Lovell  M Z Jones 《Veterinary pathology》1988,25(6):437-442

Caprine beta-mannosidosis is an autosomal recessive defect of glycoprotein catabolism with a deficiency of tissue and plasma beta-mannosidase activity and tissue accumulation of oligosaccharides within lysosomes. This rapidly fatal genetic disorder of Nubian goats is expressed at birth by a variety of clinical signs including deafness. Affected goats had folded pinnas, and the tympanic cavity was decreased due to multiple, polypoid projections of bone covered by middle ear mucosa which obstructed the view of the cochlear promontory. Numerous cells of the cochlear duct including mesothelial and epithelial cells of Reissner's membrane, mesothelial cells lining the scala tympani, cells of the stria vascularis, numerous supportive cells of the organ of Corti, cochlear hair cells, endothelial cells, perithelial cells, fibroblasts, macrophages, and neurons of the spiral ganglion contained numerous nonstaining intracytoplasmic vacuoles which resulted in distention of affected cells and caused thickening of involved structures. Ultrastructurally, the vacuoles were membrane-bound and consistent with lysosomes. Vacuolated cells were desquamated into the scala vestibuli and scala tympani. This is one of few reports describing light and electron microscopic otic alterations of a storage disease. Goats with beta-mannosidosis appear to be good models of hearing loss in patients with storage disease.  相似文献   

Toxicity of microcystin LR, a cyclic heptapeptide hepatotoxin from Microcystis aeruginosa, to rats and mice   总被引：2，自引：0，他引：2  

S B Hooser  V R Beasley  R A Lovell  W W Carmichael  W M Haschek 《Veterinary pathology》1989,26(3):246-252

Rats (Sprague-Dawley) and mice (Balb/c) were given microcystin LR intraperitoneally and were killed at intervals up to 24 hr (rats) or 90 min (mice) and necropsied. The lowest consistently lethal dose was 160 micrograms/kg in rats and 100 micrograms/kg in mice. Rats that were clinically unaffected had no lesion. All clinically affected rats in all dose groups died (from 20 to 32 hr after toxin) and had similar hepatic lesions. Livers were enlarged and dark red beginning 40 to 60 min after toxin. Mild disassociation and rounding of centrilobular hepatocytes developed within 20 min. By 60 min after toxin, degeneration and necrosis of hepatocytes involved most of the lobules except for small periportal zones. Weights of livers and kidneys were significantly increased. Eosinophilic fibrillar material filled renal glomerular capillaries as early as 9 hr after toxin. At 18 to 24 hr there was moderate vacuolation of proximal tubular epithelium with mild tubular dilatation. Beginning at 1 hr, intact hepatocytes and hepatic debris were present in pulmonary vessels. Analysis of serum revealed an increase in alanine aminotransferase 40 min after toxin; at 6 to 12 hr there were significant increases in alkaline phosphatase, total bilirubin, blood urea nitrogen, and creatinine. Mice survived only 60 to 90 min after toxin. Hepatic lesions were similar to those in rats, but renal and pulmonary lesions were not seen.  相似文献   

Rings versus knife for docking and castration of lambs     

DJ MELLOR  RJ HOLMES † 《Australian veterinary journal》1988,65(12):403-404

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A comparison of steady-state land-drainage equations     

C.J. Lovell  E.G. Youngs 《Agricultural Water Management》1984,9(1):1-21

The physical assumptions and mathematical approximations leading to ten steady-state drainage equations for installations of parallel cylindrical drains laid above a horizontal impermeable barrier, are critically examined. Water-table heights were calculated from the equations for a range of depths of impermeable barrier and of rainfall rates, using the drain's radius obtained by the hodograph analysis for infinite depth of soil. A consideration of the available solutions show that the water-table height is known with sufficient accuracy for small depths of impermeable barrier and for large depths, but at intermediate depths the solutions lead to an unsatisfactorily large uncertainty in its position. Of the drainage equations Houghoudt's equivalent depth equation, when used with the optimum drain radius given by the hodograph analysis for infinite soil depth, is the only one that gives results contained mainly within the known bounds that result from a consideration of the combination of equations.  相似文献   

Diagnostic and clinically important aspects of cyanobacterial (blue-green algae) toxicoses   总被引：2，自引：0，他引：2  

V R Beasley  A M Dahlem  W O Cook  W M Valentine  R A Lovell  S B Hooser  K Harada  M Suzuki  W W Carmichael 《Journal of veterinary diagnostic investigation》1989,1(4):359-365

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A serological survey to determine the prevalence of infection with Treponema hyodysenteriae in Western Australia     

JRL MHOMA  DJ HAMPSON  ID ROBERTSON 《Australian veterinary journal》1992,69(4):81-84

A serological survey to detect antibody titres against Treponema hyodysenteriae was conducted on pigs from 106 herds in Western Australia. Titres indicating a positive result in the tests were determined by examining 400 sera from 4 herds known to be free of swine dysentery, and sera from immunised or experimentally infected pigs. Samples of serum from 40 bacon-weight pigs from each of the 106 herds were then collected at 2 abattoirs. Each serum was tested in enzyme-linked immunosorbent assays (ELISA) against the lipopolysaccharide of T hyodysenteriae of serogroups A, B and E, respectively. To assist in evaluating the test, 19 herds were resampled and retested, and faecal samples from 17 herds were cultured for T hyodysenteriae. Thirty-five of the 106 herds (33%) had serological evidence of infection when only one batch of sera from each herd was tested. The ELISA to detect T hyodysenteriae infection in herds using 40 sera was estimated as having a sensitivity of 77.3% and a specificity of 81.8% based on the owners' opinion of their herds disease status. Prevalence of infection within herds ranged from 2.5% to 47.5%, with a mean of 18%.  相似文献   

Chronic renal failure and urolithlasis in a 2-years-old colt     

EC SADLER  DJ WITT 《Australian veterinary journal》1992,69(10):262-262

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Growth promotants in cattle in Australia   总被引：2，自引：0，他引：2  

GJ SAWYER  DJ BARKER 《Australian veterinary journal》1988,65(4):101-108

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The prevalence of intestinal spirochaetes in poultry flocks in Western Australia     

AJ McLAREN  DJ HAMPSON  SL WYLIE 《Australian veterinary journal》1996,74(4):319-321

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