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101.
New Forests - Hevea brasiliensis natural rubber production is strategic for the world economy. Meloidogyne exigua causes the main root disease in this crop in Brazil. Its early diagnosis allows...  相似文献   
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Precision Agriculture - The advance of digital agriculture combined with computational tools and Unmanned Aerial Vehicles (UAVs) has enabled the collection of data for reliably extracting...  相似文献   
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The objective was to evaluate the intake, digestibility, and ingestive sheep behavior with feeding phytogenic additives derived from plant extracts. Five non-emasculated sheep without defined breed at 28?±?1.81 kg initial body weight and 6 months age were used. Treatments consisted of administering four phytogenic additives from the garlic extracts, coriander seed, oregano, and pods of mesquite, plus a control treatment (without additive). The ration was composed of Tifton 85 hay grass, corn, soybean meal, and mineral salt. As experimental design, we used a 5?×?5 Latin square design (five treatments and five periods). The data were analyzed through the mixed model through the procedure PROC MIXED of software Systems Statistical Analysis version 9.1, with comparation analysis between the treatment without additive (control) with phytogenic additives produced from vegetable extracts of mesquite pod, of coriander seed, the bulb of garlic, and the oregano leaves. There were no significant differences for the nutrient intake and ingestive behavior patterns. However, the additive intake derived from mesquite pods and coriander extracts provided an increase in digestibility. Extracts from garlic, coriander, and mesquite pods can be used as phytogenic additives in feeding sheep.  相似文献   
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The effect of the replacement of corn silage with spineless cactus associated with Tifton 85 hay or sugarcane bagasse was evaluated on the performance, body weight components and sheep meat quality. Twenty-one Santa Inês male sheep with initial body weight of 22.9 ± 2.9 kg were used. They were confined in individual stalls for 74 days receiving the following diets: corn silage + concentrate (CS); spineless cactus + Tifton 85 hay + concentrate (TH); spineless cactus + sugarcane bagasse + concentrate (SB). After the feedlot period, the animals were slaughtered and their body components were weighed and sampled. Data were submitted to Tukey’s test at 5% probability. The average daily gain (0.25 vs 0.14 kg), cold carcass weight (13.3 vs 13.7 kg), loin muscularity (0.6 vs 0.4 kg) and liver weight (0.7 vs. 0.5 kg) were higher (P < 0.05) for the TH diet when compared to that for the CS. The physical-chemical parameters of meat were not influenced (P > 0.05) by the treatments and presented about 20.1% of crude protein, 6.1% of crude fat and shear force of 2.0 kgf/cm2. In complete diets for sheep, Tifton 85 hay associated with spineless cactus provides greater weight gain, cold carcass muscularity and organ weight when compared to those for animals fed with corn silage as exclusive roughage. Sugarcane bagasse associated with spineless cactus may replace corn silage in complete diets for sheep.  相似文献   
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The Rickettsiales Ehrlichia ruminantium (ER) is the causative agent of heartwater, a fatal tick-borne disease of livestock in sub-Saharan Africa and in the Caribbean, posing strong economical constraints to livestock production. In an attempt to identify the most prominent proteins expressed by this bacterium, especially those encoded by the major antigenic protein 1 (map1) multigene family, a proteome map of ER cultivated in endothelial cells was constructed by using two dimensional gel electrophoresis combined with mass spectrometry. Among the sixty-four spots detected, we could identify only four proteins from the MAP1-family; the other proteins detected were mainly related to energy, amino acid and general metabolism (26%), to protein turnover, chaperones and survival (21%) and to information processes (14%) or classified as hypothetical proteins (23%). Additional studies on MAP1-family protein using immunochemical labeling also revealed that these proteins are differentially expressed along the bacterium life cycle, presenting different structural organization. Interestingly, when infectious elementary bodies (EBs) are released from host cells, MAP1 appears to be organized in SDS and heat-resistant dimers and trimers stabilized by disulfide bridges. Overall, the results presented herein not only reveal the first partial proteome map of ER but provide new insights on the expression ER MAP1-family proteins in host endothelial cells.  相似文献   
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ABSTRACT: Susceptibility of sheep to scrapie, a transmissible spongiform encephalopathy of small ruminants, is strongly influenced by polymorphisms of the prion protein gene (PRNP). Breeding programs have been implemented to increase scrapie resistance in sheep populations; though desirable, a similar approach has not yet been applied in goats. European studies have now suggested that several polymorphisms can modulate scrapie susceptibility in goats: in particular, PRNP variant K222 has been associated with resistance in case-control studies in Italy, France and Greece. In this study we investigated the resistance conferred by this variant using a natural Italian goat scrapie isolate to intracerebrally challenge five goats carrying genotype Q/Q 222 (wild type) and five goats carrying genotype Q/K 222. By the end of the study, all five Q/Q 222 goats had died of scrapie after a mean incubation period of 19 months; one of the five Q/K 222 goats died after 24 months, while the other four were alive and apparently healthy up to the end of the study at 4.5 years post-challenge. All five of these animals were found to be scrapie negative. Statistical analysis showed that the probability of survival of the Q/K 222 goats versus the Q/Q 222 goats was significantly higher (p = 0.002). Our study shows that PRNP gene mutation K222 is strongly associated with resistance to classical scrapie also in experimental conditions, making it a potentially positive target for selection in the frame of breeding programs for resistance to classical scrapie in goats.  相似文献   
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Melatonin has been reported to play a fundamental role in T-cell immunoregulation. Control of Trypanosoma cruzi parasitism during the acute phase of infection is considered to be critically dependent on direct macrophage activation by cytokines. The aim of this work was to evaluate the influence of exogenous melatonin treatment and the influences exerted by sexual hormones during the acute phase of the experimental Chagas' disease in rats. With melatonin treatment, orchiectomized animals (CMOR and IMOR) displayed the highest concentrations of IFN-γ and TNF-α. On the 7th day post-infection, untreated and treated orchiectomized animals (IOR and IMOR) showed an enhanced number of peritoneal macrophages. Nitric oxide levels were also increased in untreated and treated orchiectomized (IOR and IMOR) when compared to the other groups, with or without LPS. Our data suggest that melatonin therapy associated with orchiectomy induced a stimulating effect on the immune response to the parasite.  相似文献   
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