Molecular characterization of Canis familiaris oral papillomavirus 1 identified in naturally infected dogs from Northeast Brazil     

Jordana D. R. Reis  Luana B. Oliveira  Lucas A. B. O. Santos  Rosilene C. Soares  Marcus V. A. Batista 《Veterinary dermatology》2019,30(5):424-e128

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Evaluation of cardiorespiratory and biochemical effects of ketamine‐propofol and guaifenesin‐ketamine‐xylazine anesthesia in donkeys (Equus asinus)     

Cássia M Molinaro Coelho  Juan C Duque Moreno  Daniel da S Goulart  Leandro B Caetano  Lorena K Soares  Gustavo H Coutinho  Geraldo ES Alves  Luiz Antonio F da Silva 《Veterinary anaesthesia and analgesia》2014,41(6):602-612

ObjectivesTo evaluate the cardiorespiratory and biochemical effects of ketamine-propofol (KP) or guaifenesin-ketamine-xylazine (GKX) anesthesia in donkeys.Study designProspective crossover trial.AnimalsEight healthy, standard donkeys, aged 10 ± 5 years and weighing 153 ± 23 kg.MethodsDonkeys were premedicated with 1.0 mg kg^?1 of xylazine (IV) in both treatments. Eight donkeys were administered ketamine (1.5 mg kg^?1) and propofol (0.5 mg kg^?1) for induction, and anesthesia was maintained by constant rate infusion (CRI) of ketamine (0.05 mg kg^?1 minute^?1) and propofol (0.15 mg kg^?1 minute^?1) in the KP treatment. After 10 days, diazepam (0.05 mg kg^?1) and ketamine (2.2 mg kg^?1) were administered for induction, and anesthesia was maintained by a CRI (2.0 mL kg^?1 hour^?1) of ketamine (2.0 mg mL^?1), xylazine (0.5 mg mL^?1) and guaifenesin (50 mg mL^?1) solution. Quality of anesthesia was assessed along with cardiorespiratory and biochemical measurements.ResultsAnesthetic induction took longer in GKX than in KP. The induction was considered good in 7/8 with KP and in 6/8 in GKX. Anesthetic recovery was classified as good in 7/8 animals in both treatments. Xylazine administration decreased heart rate (HR) in both treatments, but in KP the HR increased and was higher than GKX throughout the anesthetic period. Respiratory rate was higher in GKX than in KP. PaO₂ decreased significantly in both groups during the anesthetic period. Glucose concentrations [GLU] increased and rectal temperature and PCV decreased in both treatments. Arterial lactate [LAC] increased at recovery compared with all time points in KP. [GLU] and calcium were higher in GKX than in KP at recovery.Conclusion and clinical relevanceThese protocols induced significant hypoxemia but no other cardiorespiratory or metabolic changes. These protocols could be used to maintain anesthesia in donkeys, however, they were not tested in animals undergoing surgery.  相似文献   

Performance of dual and triple fungicide premixes for managing soybean rust across years and regions in Brazil: A meta-analysis     

Jhonatan P. Barro  Kaique S. Alves  Cláudia V. Godoy  Alfredo R. Dias  Carlos A. Forcelini  Carlos M. Utiamada  Edson R. de Andrade Júnior  Fernando C. Juliatti  Fernando J. Grigolli  Heraldo R. Feksa  Hercules D. Campos  Ione C. P. V. Chaves  Ivan P. Araújo Júnior  João Mauricio T. Roy  José Nunes Júnior  Luana M. R. Belufi  Luciana C. Carneiro  Luís H. C. P. Silva  Marcelo G. Canteri  Marcio M. Goussain Júnior  Marina Senger  Maurício C. Meyer  Moab D. Dias  Mônica A. Müller  Mônica C. Martins  Mônica P. Debortoli  Nédio R. Tormen  Silvânia H. Furlan  Tiago F. Konageski  Valtemir J. Carlin  Wilson S. Venâncio  Emerson M. Del Ponte 《Plant pathology》2021,70(8):1920-1935

Soybean rust in Brazil is currently controlled with several commercial fungicide premixes composed of demethylation inhibitors (EPOXiconazole, CYPRoconazole, PROThioconazole, TEBUconazole), quinone-outside inhibitors (AZOXystrobin, TriFLoXystrobin, PYRAclostrobin, PICOxystrobin), and succinate demethylation inhibitors (BENZovindiflupyr, BIXaFen, FLUXapyroxad). Here, we summarize the performance of eight premixes evaluated in 177 cooperative trials conducted in 46 locations across 10 states from 2015 to 2020. All fungicide treatments were sprayed three times starting at R1/R2. Percentage control (, %), from back-transforming meta-analytic estimates of the log of the ratio, ranged from 56.2% (PICO + CYPR) to 76.8% (BIXF + TFLX + PROT). Estimates of mean yield difference (, kg/ha) between fungicide-treated and untreated plots were greatest for BIXF + TFLX + PROT (1,080) followed by PICO + BENZ (1,010), PYRA + EPOX + FLUX (981.5), AZOX + BENZ (910), TFLX + PROT (891), PICO +TEBU (682), TFLX + CYPR (646), and PICO + CYPR (600). Significant declines in both and in as little as 4 years were detected for AZOX + BENZ (35.3%; 550 kg/ha) and PICO + BENZ (15.5%; 359.8 kg/ha). Variance in was reduced by the inclusion of baseline severity as covariate. In trials where baseline disease was ≥70%, yield was 250 kg/ha greater compared to areas with low baseline disease. Disease control and yield response were generally greater in the south-east, where the frequency of profitable scenarios was 30% higher on average than in the north-west. Results of this meta-analysis are critical for supporting decisions during planning of fungicide programmes.  相似文献   

Damage by Homalinotus depressus in commercial coconut palm crops in the Amazonian region of Brazil     

Rafael Coelho Ribeiro  Walkymário de Paulo Lemos  Paulo Manoel Pontes Lins  Roberta de Melo Valente  Antonio Agostinho Müller  Alexandre Mehl Lunz  José Cola Zanuncio 《Phytoparasitica》2012,40(3):239-241

The coconut palm (Cocos nucifera L., Arecaceae) has great economic and social importance for many industrial and non-industrial products. Pests can reduce its productivity and cause its death. In 2005, larvae and adults of a Curculionidae were observed damaging the floral stalk of coconut palms in commercial plantations in the municipality of Moju, Pará State, in the Brazilian Amazon. Insects were identified as the black coconut bunch weevil, Homalinotus depressus (L.) (Coleoptera: Curculionidae: Cholini). This is the first report of this pest damaging coconut palms in Brazil.  相似文献   

Feline Immunodeficiency Virus Subtype B in Domestic Cats in Minas Gerais, Brazil     

Caxito FA  Coelho FM  Oliveira ME  Resende M 《Veterinary research communications》2006,30(8):953-956

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Effects of Topical Application of Sunflower-Seed Oil on Experimentally Induced Wounds in Horses     

Luiz Antonio T. Oliveira Jr.  Vinicius R.C. Souza  Denise C. Endringer  Dean A. Hendrickson  Clarisse S. Coelho 《Journal of Equine Veterinary Science》2012

The objectives were to evaluate clinical and histopathological aspects of topical application of sunflower-seed oil (Helianthus annuus) on the healing process of experimentally induced wounds in lumbar and metacarpal areas of healthy horses. Six adult horses were used. Eight wounds were surgically produced on each horse: two 6.25-cm² wounds cranial to the sacrum on each side of the lumbar region and two 2.89-cm² wounds close to the proximal epiphysis of the metacarpus on the lateral aspect of each forelimb. Left side was used for macroscopic observations and right side for histopathological analysis. The experimental lesions were treated daily with sunflower-seed oil, whereas saline solution was used in control lesions. Macroscopic and histopathological analyses were performed on tissue harvested at 3, 7, 14, and 21 days. Complete healing time for all wounds was recorded. For lumbar region’s wounds, a contraction of 90.78% was recorded for those treated with oil and of 79.27% for control wounds after 21 days of treatment. For metacarpal region’s wounds, a contraction of 47.63% was recorded for wounds treated with oil and of 30.21% for control wounds. Wounds in the sunflower-seed oil treatment group had an elevation of polymorphonuclear cells, a newly formed vascular bed during the inflammatory phase, and a better alignment of collagen fibers during the remodeling phase. In conclusion, topical application of sunflower-seed oil was beneficial in the healing process of experimentally induced skin wounds in horses, with best results for treatment of lumbar wounds, making it a therapeutic option in equine wound healing.  相似文献   

Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli isolates in faecal samples of broilers     

Daniela Costa  Laura Vinu  Patricia Poeta  Ana Cludia Coelho  Manuela Matos  Yolanda Senz  Sergio Somalo  Myriam Zarazaga  Jorge Rodrigues  Carmen Torres 《Veterinary microbiology》2009,138(3-4):339-344

Seventy-six faecal samples were obtained from broilers at slaughterhouse level in Portugal. Samples were inoculated on cefotaxime-supplemented Levine agar plates. Cefotaxime-resistant Escherichia coli isolates were recovered from 32 samples (42.1%), obtaining a total of 34 E. coli isolates (one or two isolates per sample). Susceptibility to 16 antibiotics was studied by disk diffusion method, and 85% of the isolates presented a phenotype of multi-resistance that included antimicrobial agents of at least four different families. Extended-spectrum-beta-lactamases (ESBL) of the TEM and CTX-M groups were detected in 31 ESBL-positive E. coli isolates. Twenty-six isolates harboured the bla_TEM-52 gene and two of them also harboured bla_TEM-1b. The bla_CTX-M-14 gene was identified in three isolates (in association with bla_TEM-1b in one of them), and bla_CTX-M-32 was demonstrated in two additional isolates. Three of the 34 cefotaxime-resistant isolates (9%) did not produce ESBLs, and two of them presented mutations at positions −42 (C → T), −18 (G → A), −1 (C → T), and +58(C → T) of the promoter/attenuator region of ampC gene. tet(A) and/or tet(B) genes were detected in all 34 tetracycline-resistant isolates, aadA in all 26 streptomycin-resistant isolates; cmlA in 3 of 6 chloramphenicol-resistant isolates, and aac(3)-II or aac(3)-I + aac(3)-IV genes in all 4 gentamicin-resistant isolates. Different combinations of sul1, sul2 and sul3 genes were demonstrated among the 22 trimethoprim–sulfamethoxazole-resistant isolates. Amino acid changes in GyrA and ParC proteins were identified in all 18 ciprofloxacin-resistant isolates. The results of this study indicate that the intestinal tract of healthy poultry is a reservoir of ESBL-positive E. coli isolates.  相似文献   

Assessing genetic variability in germplasm of Phaseolus vulgaris L. collected in Northern Portugal     

Rita C. Coelho  Miguel A. Faria  Joana Rocha  Aida Reis  Maria Beatriz P.P. Oliveira  Eugénia Nunes 《Scientia Horticulturae》2009

The common bean (Phaseolus vulgaris L.) is the most consumed legume in the world. In Portugal, the geographic isolation of the region surrounded by the mountainous barrier of Peneda-Gerês, Barroso and Marão is thought to have the safeguard of a large number of adapted bean populations. In order to assess the value of this germplasm to breeding programs, a study of 20 Portuguese landraces collected in this Northern region were evaluated for agronomical (days to flowering, plant height, days to harvest, weight of seeds per plant and 100 seeds weight), chemical (crude protein) and genetic diversity (microsatellite DNA).  相似文献   

Prevalence of Ehrlichia canis infection in thrombocytopenic dogs from Rio de Janeiro, Brazil     

Macieira Dde B  Messick JB  Cerqueira Ade M  Freire IM  Linhares GF  Almeida NK  Almosny NR 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2005,34(1):44-48

BACKGROUND: Infection with Ehrlichia canis causes a highly variable, multisystemic disease in dogs. Nevertheless, many clinicians in Rio de Janeiro, Brazil, use the presence of only thrombocytopenia to make a presumptive diagnosis of E canis infection. OBJECTIVE: The objective of this study was to determine the prevalence of E canis in thrombocytopenic dogs from Rio de Janeiro, Brazil, using polymerase chain reaction (PCR). METHODS: Following DNA extraction of whole blood samples from 226 dogs, PCR assays were done using primers for rickettsial DNA (including Ehrlichia spp, Anaplasma platys and A phagocytophilum) and using E canis-specific primers (16S rRNA gene). Dogs were grouped as thrombocytopenic and nonthrombocytopenic based on platelet counts. The null hypothesis that there was no difference in the prevalence of E canis in these groups was rejected at P<.05. RESULTS: Thirty-six (32.1%) of the thrombocytopenic dogs and 4 (3.5%) of the nonthrombocytopenic dogs were positive for rickettsial gene sequences (P<.0001). Further, 30 (26.8%) of thrombocytopenic dogs and 4 (3.5%) nonthrombocytopenic dogs were positive for E canis-specific gene sequences (P<.0001). CONCLUSIONS: Although the prevalence of E canis infection was higher in thrombocytopenic dogs, less than one third of these dogs had demonstrable E canis infection. Thus, thrombocytopenia is not specific for the detection of E canis infection and should not be used solely to establish a diagnosis of canine ehrlichiosis, even in a geographic area with relatively high disease prevalence.  相似文献   

Relationships Between Angular Leaf Spot, Healthy Leaf Area, Effective Leaf Area and Yield of Phaseolus vulgaris     

W.C. Jesus Junior  F.X.R. Vale  R.R. Coelho  P.A. Paul  B. Hau  A. Bergamin Filho  L. Zambolim  R.D. Berger 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(6):625-632

Three field experiments were carried out with the bean cultivar Carioca Comum to investigate the relationships among visual and virtual severity of angular leaf spot (caused by Phaeoisariopsis griseola), area under visual and virtual disease progress curves (AUDPC), healthy leaf area index on any given day (HLAI), healthy leaf area duration (HAD), healthy leaf area absorption (HAA), effective leaf area duration (ELAD), effective leaf area absorption (ELAA) and yield of Phaseolus vulgaris. To obtain a wide range of disease severities, the plots were sprayed with fungicide at different stages of plant growth (before, during and after flowering). Visual and virtual severity and AUDPC showed no significant correlation with yield. However, HAD, HAA, ELAD and ELAA were significantly correlated with yield. Variables that considered the effective leaf area (ELAD and ELAA) provided similar or better coefficients of determination (R²) than those that considered the remaining green leaf area only (HAD and HAA). Single-point models with HLAI, effective leaf area index (ELAI), intercepted radiation by healthy leaf area (HRI) and intercepted radiation by effective leaf area (EHRI) to estimate yield at various times during the crop season were developed. The slope of the relationship between yield and HLAI, ELAI, HRI and EHRI proved to be stable, regardless of planting date and bean growth stage (from R6 to R8).  相似文献