SCAR and CAPS markers flanking the Brassica oleracea L. Pp523 downy mildew resistance locus demarcate a genomic region syntenic to the top arm end of Arabidopsis thaliana L. chromosome 1     

Mário Farinhó  Paula Coelho  António Monteiro  José Leitão 《Euphytica》2007,157(1-2):215-221

We recently mapped the Pp523 locus that includes a single, dominant gene conferring resistance to downy mildew expressed in adult plants to a 75.1 cm long linkage group on a genetic linkage map of Brassica oleracea L. More recently, we identified a new AFLP marker 2.8 cm downstream from the resistance gene. The five DNA markers within an 8.5 cm region encompassing the Pp523 gene were cloned and sequenced. Three of these markers were transformed into SCARs (sequence characterised amplified regions), however, two among them were monomorphic and were analysed as CAPS (cleaved amplified polymorphic sequence) markers among the mapping population. Searched against genomic databases, the five B. oleracea DNA-marker sequences matched Arabidopsis thaliana L. gene sequences that delimit a conserved syntenic region in the top arm end of chromosome 1 of this last species. Considering the close genetic relatedness between both species, the information on this specific genomic region in A. thaliana is particularly useful for the construction of a fine-scale map of the corresponding genomic region in B. oleracea. The identified SCAR and CAPS markers can be used for marker assisted selection (MAS) in breeding programs aimed at the introgression of the Pp523 resistance locus, allowing the reliable indirect identification of plants harbouring the resistance gene with a margin of error of approximately six in ten-thousand selected plants.  相似文献   

Impact of urban development on streamflow regime of a Portuguese peri-urban Mediterranean catchment     

Carla Sofia Santos Ferreira  Rory Peter Dominic Walsh  João Pedro Carvalho Nunes  Tammo S. Steenhuis  Manuel Nunes  João Luís Mendes Pedroso de Lima  Celeste Oliveira Alves Coelho  António José Dinis Ferreira 《Journal of Soils and Sediments》2016,16(11):2580-2593

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Novel protein from Labramia bojeri A. DC. seeds homologue to Kunitz-type trypsin inhibitor with lectin-like properties     

Macedo ML  Freire Md  Martins LT  Martinez DS  Gomes VM  Smolka MB  Toyama MH  Marangoni S  Coelho LC 《Journal of agricultural and food chemistry》2004,52(25):7548-7554

This study starts by isolating and characterizing the first protein from Labramia bojeri seeds, which belong to the Sapotaceae family. The purified lectin analyzed by SDS-PAGE with and without beta-mercaptoethanol shows two protein bands (M(r) = 19 and 20 kDa), which cannot be resolved. Protein bands have shown similar characteristics as molecular masses, determined by gel filtration and native gel; N-terminal sequences presented a difference in their isoelectric points. We have suggested that those protein bands might be variants of the protein named Labramin. The sequence database search has shown that the N-terminal sequence of Labramin presented a high degree of homology to Kunitz-type trypsin inhibitor (82-52%) despite no trypsin inhibition activity detection. The lectin-like form from Labramin was better inhibited by glycoproteins and has also presented growth inhibition of the fungus Colletotrichum lindemuthianum and the yeast Saccharomyces cerevisiae, but it has not presented an apparent effect on Fusarium oxysporum.  相似文献   

增加维生素饲喂量有利于瘦肉生长     

沈建仁  华为  Mike Coelho 《国外畜牧学(猪与禽)》2001,(6):13-15

当前猪的日粮维生素需要量估计值根据的都是2 0～ 40年前所作研究的结果。然而 ,与 2 0年前相比 ,瘦肉型快速生长基因型的培育以及新颖管理措施的采用 ,使猪的蛋白质沉积率提高了 80 %。低免疫状态的猪可以每天沉积蛋白质 2 0 0克 ,这一沉积率是常规饲养猪的二倍。根据每单位体蛋白沉积对维生素需要量的估计来看 ,具有高瘦肉阻止生长能力的猪 ,对Ｂ族维生素的需要量可能是当前ＮＲＣ推荐量的 4＆ 8倍。美国学者Ｓｔａｈｌｙ和Ｃｏｏｋ(1 996)研究了免疫状态的影响 ,试验采用两种不同的管理体系 ,分别创造一种中等水平和高水平的抗原接触环境…  相似文献   

早熟蛋鸡的管理需要改变     

刘建辉  洪成斌  Michael Coelho 《国外畜牧学(猪与禽)》2001,(6):32-36

商品新母鸡的成熟日龄日益提前 ,产蛋量和蛋重也不断增加 ,因此对其营养管理也需要加以改变以适应新品系的遗传潜力  相似文献   

Implications of carbohydrate sources and rate of body weight gain on puberty in ewe lambs in tropical climate conditions     

Ferreira  Evandro Maia  de Castro Ferraz  Marcos Vinicius  Biava  Janaina Socoloviski  de Assis  Rhaíssa Garcia  Barroso  José Paulo Roman  Polizel  Daniel Montanher  de Araujo  Leandro Coelho  Pires  Alexandre Vaz 《Tropical animal health and production》2020,52(1):373-378

Tropical Animal Health and Production - The objectives were to evaluate the effects of two non-fibrous carbohydrate sources and the rate of body weight gain on puberty status in ewe lambs. Sixty...  相似文献   

New data about home range and movements of Oligoryzomys flavescens (Rodentia: Cricetidae) help to understand the spread and transmission of Andes virus that causes Hantavirus Pulmonary Syndrome     

Malena Maroli  Beln Crosignani  Carlos I. Pia  Rocío Coelho  Valeria P. Martínez  Isabel Elisa Gmez Villafae 《Zoonoses and public health》2020,67(3):308-317

Hantavirus pulmonary syndrome is an emerging infectious disease caused by viruses of the genus Orthohantavirus. The rodent Oligoryzomys flavescens is distributed along four countries of South America. In Argentina, O. flavescens acts as a reservoir of three genotypes of ANDV orthohantavirus. The aims of this work were to estimate home range size and movements—with spool‐and‐line and radiotelemetry—of infected and non‐infected O. flavescens in order to understand the spread and transmission of the virus. O. flavescens use a wide area to satisfice its requirements, reaching a home range of 1.82 ha during spring. Orthohantavirus infection did not change the behaviour of individuals. We observed a great overlapping in the home range of infected and non‐infected individuals resulting in a high probability of virus dispersion on rodent population. These results show that human health risks could be high on island environments and knowledge about the movement ecology of O. flavescens provides useful information on prevention.  相似文献   

Incidence-severity relationships in non-treated and fungicide-treated wheat head blast epidemics in Brazil     

dos Santos  Gustavo Bilibio  de Oliveira Coelho  Maurício Antonio  Del Ponte  Emerson Medeiros 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(4):1003-1010

European Journal of Plant Pathology - The relationship between wheat head blast incidence (I; proportion of diseased heads in a sample) and severity (S; the average diseased area in a sample of...  相似文献   

Assessment of Mercury in Water, Sediments and Biota of a Southern European Estuary (Sado Estuary, Portugal)     

Ana I. Lilleb?  Pedro J. Coelho  Pedro Pato  M??nica V??lega  Rui Margalho  M??rio Reis  Jos?? Raposo  Eduarda Pereira  Armando C. Duarte  Miguel A. Pardal 《Water, air, and soil pollution》2011,214(1-4):667-680

The aim if this study was to assess the distribution of mercury in water, suspended particulate matter, surface sediments and biota from the Sado estuary, which, for the most part, is classified as a natural reserve, so as to evaluate its environmental quality status in terms of mercury contamination. Besides the diffuse sources of mercury coming into the Sado estuary, there are also additional contributions from the northern industrial zone and from the urban areas within the system. Applying national and international guidelines to different environmental matrices, the results obtained show that the system does not seem to be under environmental risk as far as mercury contamination is concerned. These quality guidelines can be used to rank and prioritise sites of concern. Hence, the area at the northern industrialised area deserves particular attention. The concentration of mercury in sediments of this area (0.54?mg kg^?1) simultaneously succeeded the European Union Water Framework Directive (EU-WFD) reference value, the OSPAR Convention Ecotoxicological Assessment Criteria (EACs) and is classified as class 2 in degree of contamination by the National legislation, which implies some legal restrictive rules. Considering the stations close to urbanised areas, one exceeded the OSPAR EACs for dissolved mercury, whilst the other exceeded the EU-WFD reference value for mercury concentrations in sediments. No statistical significant relations were found between mercury concentrations in biota (Ulva sp, Hediste diversicolor, Scrobicularia plana, Cerstoderma edule and Carcinus maenas) and in the abiotic matrices (sediment and water column, including mercury in its dissolved form and associated to suspended particulate matter). This paper provides an overview of the guidelines for Hg proposed for a considerable number of coastal systems of the northern hemisphere and highlights the complex interactions of Hg in the different environmental compartments in low contaminated systems.  相似文献   

Anti-inflammatory Properties of Orange Juice: Possible Favorable Molecular and Metabolic Effects   总被引：1，自引：1，他引：0  

Raquel Cristina Lopes Assis Coelho  Helen Hermana M. Hermsdorff  Josefina Bressan 《Plant foods for human nutrition (Dordrecht, Netherlands)》2013,68(1):1-10

The low-grade inflammation has been recognized as the link between adiposity and the risk of chronic metabolic disorders. Thus, increased concentrations of inflammatory markers, such as interleukins and tumor necrosis factor alpha have been found in obese individuals. In turn, diet can positively or negatively influence on the risk of chronic metabolic diseases by modulating the inflammatory status. In this context, orange juice consumption can play a role in modulation of inflammatory markers through bioactive compounds, such as the flavonoids (hesperidin, naringenin). According to this review, orange juice appears to mediate the inflammatory response in plasma level and gene expression, and in postprandial and chronic (≥7 consecutive days) periods. The current findings suggest that orange juice could be a dietary feature for prevention and treatment of chronic diseases, although more studies are necessary to evaluate the physiological and molecular mechanisms involved.  相似文献