Molecular epidemiological analyses of the teratogenic Aino virus based on the sequences of a small RNA segment     

Yamakawa M  Yanase T  Kato T  Tsuda T 《Veterinary microbiology》2008,129(1-2):40-47

The sequences of a small RNA segment of Aino virus isolates were analyzed to define the molecular epidemiology and genetic relationships to other species in the genus Orthobunyavirus in the family Bunyaviridae. The nucleotide and amino acid sequences of the segment were highly conserved among strains isolated from 1964 to 2002 in Japan. These Japanese isolates were segregated into two distinct lineages, one containing the prototype strain JaNAr28 isolated in 1964 and the other containing strains isolated after 1986, by phylogenetic analysis based on the nucleocapsid gene sequences. Japanese strains isolated after 1986 were rather more closely related to Kaikalur virus isolated in India in 1971 than to strain JaNAr28. On the other hand, an Australian strain, B7974, was closely related to Peaton virus. The B7974 strain might have been generated by inter-serotype genetic reassortment between Aino and Peaton viruses in Australia during their evolution. However, recent Aino virus strains isolated in Japan appear to be genetically stable.  相似文献   

In vitro differentiation of canine celiac adipose tissue-derived stromal cells into neuronal cells   总被引：2，自引：0，他引：2  

Sago K  Tamahara S  Tomihari M  Matsuki N  Asahara Y  Takei A  Bonkobara M  Washizu T  Ono K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(4):353-357

To investigate in vitro differentiation of canine adipose tissue-derived stromal cells (ATSCs) into neuronal cells, ATSCs from celiac adipose tissue in clinically healthy beagle dogs were treated with 100 muM dibutyryl cyclic adenosine monophosphate (dbcAMP) and 125 muM isobuthylmethylxanthine (IBMX). ATSCs were morphologically changed into differentiated ATSCs from spindle-shaped cells to neuron-like cells with numerous processes after the treatment. Expression of neuron-specific enolase (NSE) as an early neuron specific marker protein was detected in both ATSCs and differentiated ATSCs, however diachronic increase of NSE expression was observed in differentiated ATSCs after the treatment with dbcAMP/IBMX. In addition, neurofilament-68 (NF-68) as an early to mature neuron specific marker protein was weakly expressed in differentiated ATSCs. Neuron specific glutamate and glucose transporter (EAAC1 and GLUT-3, respectively) mRNAs were strongly expressed in differentiated ATSCs compared with those in ATSCs, although glia specific glutamate transporter mRNA (GLT-1) was also detected in differentiated ATSCs. ATSCs can differentiate into early to mature neuronal cells and are candidate cells for autologous nerve regeneration therapy, although additional research is needed to examine functional characteristics of differentiated ATSCs.  相似文献   

A case report of a renal mixed epithelial and stromal tumor in a heterozygous S1P2 receptor deficient mouse     

Takabatake M  Takuwa Y  Takuwa N  Yasuno H  Matsumoto S  Shibutani M  Mitsumori K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):483-485

We report a case of mixed epithelial and stromal tumor of the kidney (MESTK) in a 32-week-old heterozygous sphingosine 1-phosphate-2 (S1P2) receptor deficient female mouse. A white solid mass replacing the left kidney was observed at the left retroperitoneal wall. Histologically, the tumor mass consisted of dimorphic cellular components of epithelial and stromal cells. Epithelial cells formed various sized irregular-shaped tubular structures resembling renal tubules surrounded by stromal cells. Immunohistochemically, epithelial cells were positive for cytokeratin, while stromal cells showed positive immunoreactivity with alpha-smooth muscle actin as well as vimentin. Based on the morphological and immunohistochemical findings, this tumor was diagnosed as a MESTK.  相似文献   

Crop rotation with broccoli suppresses Verticillium wilt of eggplant     

Kentaro Ikeda  Shinpei Banno  Akiko Furusawa  Satoshi Shibata  Kazuhiro Nakaho  Makoto Fujimura 《Journal of General Plant Pathology》2015,81(1):77-82

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吉林省白杨锈菌鉴定     

谢昀烨  纪景欣  李壮  王琦  李玉  柿嶌眞 《植物病理学报》2016,46(2):280-284

 银白杨,杨属植物,广泛种植于中国东北部地区,其锈病发生特别严重。在本研究中,根据形态特征和生活史将白杨锈菌鉴定为Melampsora laricis。使用杨树锈病叶片上的担孢子对日本落叶松及白山落叶松进行接种试验,结果证实了其精子器和锈子器世代发生在落叶松植物上。  相似文献   

Resistance to <Emphasis Type="Italic">Phytophthora infestans</Emphasis>: exploring genes required for disease resistance in Solanaceae plants     

Daigo Takemoto  Yusuke Shibata  Makoto Ojika  Yuri Mizuno  Sayaka Imano  Mina Ohtsu  Ikuo Sato  Sotaro Chiba  Kazuhito Kawakita  Soriya Rin  Maurizio Camagna 《Journal of General Plant Pathology》2018,84(5):312-320

The oomycete Phytophthora infestans is the causal agent of potato late blight, one of the most destructive and historically significant pathogens in agricultural production. A virus-induced gene silencing-based screening of the solanaceous model plant N. benthamiana resulted in revealing a wide range of resistance mechanisms of solanaceous plants against this pathogen. In this article, we present an overview of the various pathways involved in the N. benthamiana–P. infestans pathosystem, including some of the follow-up work that was triggered by these findings. The purpose of this review is to assemble these findings and integrate them into our current understanding of plant pathogen defense mechanisms and discuss their potential application for the development of potato resistance to P. infestans.  相似文献   

Development of the musculature and muscular abnormalities in larval seven-band grouper Epinephelus septemfasciatus     

Susumu Uji  Tohru Suzuki  Takashi Iwasaki  Kazuhisa Teruya  Keiichi Hirasawa  Makoto Shirakashi  Shizumasa Onoue  Yoichi Yamashita  Koichi Okuzawa 《Fisheries Science》2013,79(2):277-284

There are a number of reports documenting skeletal development in groupers, but surprisingly little is known about muscle development. We have documented muscle development in the seven-band grouper Epinephelus septemfasciatus (Thunberg), focusing primarily on the cranial muscles from 0 days post-hatching (dph) to 28 dph, using a modified whole mount immunohistochemical staining method. The eye muscles developed first followed by the cheek muscles, which completed development prior to first feeding. The muscles associated with the opercle and the levator internus 1 developed gradually and were not linked to first feeding. Later, between 12 and 16 dph, the muscle composition in the dorsal branchial arches changed to the adult form. We observed a number of cultured larvae that exhibited abnormal musculature in the body and jaw. Our results provide the first documentation of muscle development in larval seven-band grouper. This information can be used to detect abnormalities during development and provide a marker to assess the effect of changes in larval rearing techniques.  相似文献   

Effect of High β-Glucan Barley on Serum Cholesterol Concentrations and Visceral Fat Area in Japanese Men—A Randomized,Double-blinded,Placebo-controlled Trial     

Shimizu C  Kihara M  Aoe S  Araki S  Ito K  Hayashi K  Watari J  Sakata Y  Ikegami S 《Plant foods for human nutrition (Dordrecht, Netherlands)》2008,63(1):21-25

This study investigated whether the consumption of a diet in which high-β-glucan barley replaced rice would reduce the visceral fat area as well as the serum low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) in hypercholesterolemic Japanese men. A randomized, double-blinded, placebo-controlled intervention study was conducted in 44 hypercholesterolemic Japanese men with a body mass index (BMI) >22 kg/m². The subjects were randomly assigned to groups consuming either rice (placebo group) or a mixture of rice and pearl barley with a high β-glucan content (test group, 7.0 g β-glucan per day) for 12 weeks. Blood samples were taken, and CT scan obtained before the trial and every four weeks during the trial. The pearl barley intake significantly reduced serum concentrations of LDL-C (P = 0.041) and TC (P = 0.037) during the trial. Significant differences between the test and placebo groups were found for the visceral fat (P = 0.039), BMI (P = 0.015), and waist circumference (P = 0.011) at the end point. The consumption of pearl barley with a high β-glucan content reduces not only LDL-C but also visceral fat area.  相似文献   

Molecular structures of gellan gum imaged with atomic force microscopy in relation to the rheological behavior in aqueous systems in the presence or absence of various cations     

Funami T  Noda S  Nakauma M  Ishihara S  Takahashi R  Al-Assaf S  Ikeda S  Nishinari K  Phillips GO 《Journal of agricultural and food chemistry》2008,56(18):8609-8618

Aqueous solutions of gellan gum with comparable molecular masses but with different acyl contents were investigated by atomic force microscopy and rheological measurements in the presence or absence of various cations. For a high-acyl sample, no continuous network structures were identified microscopically, except in the presence of Ca (2+), where structural inhomogeneity was the highest in terms of the height distribution of molecular assemblies. Rheological thermal hysteresis between sol-gel transitions was detected in the presence of K (+) and Ca (2+), particularly Ca (2+). The storage modulus at 20 degrees C was larger in the order Na (+) < Ca (2+) < K (+). For a low-acyl sample, continuous network structures were identified in the presence of each cation, involving greater thermal hysteresis than the corresponding data for the high-acyl sample. Structural homogeneity was the highest in the presence of K (+). Thermal hysteresis and elasticity of the system were discussed in terms of continuousness and homogeneity of network structures.  相似文献   

Transfer and accumulation of persistent organochlorine compounds from bovine dams to newborn and suckling calves     

Hirako M 《Journal of agricultural and food chemistry》2008,56(15):6768-6774

The maternal-fetal and neonatal transfers of polychlorinated dibenzo- p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and dioxin-like polychlorinated biphenyls (PCBs) were measured in the blood and milk of dams and in the blood of newborn and suckling calves. Calf blood toxic equivalent quantities (TEQs) were drastically increased by suckling. Blood concentrations of individual congeners were greater in suckling calves than in newborn calves, excluding octachlorodibenzo- p-dioxin (O 8CDD); O 8CDD did not readily transfer to milk but was readily absorbed by the gastrointestinal tract and remained in systemic circulation longer than other congeners. Congener concentrations in milk were correlated with maternal blood levels, and those in suckling calf blood were dependent on their concentrations in milk. These results suggest that neonatal calves absorb more lipophilic organochlorine compounds than prenatal fetuses, that those compounds that are transferred from dams to calves are associated not only with lipid transport but also with other carriers, and that the distribution of congeners is structure-dependent.  相似文献