Fish anesthesia: effects of the essential oils of Hesperozygis ringens and Lippia alba on the biochemistry and physiology of silver catfish (Rhamdia quelen)     

Cândida Toni  Alexssandro Geferson Becker  Larissa Novaes Simões  Carlos Garrido Pinheiro  Lenise de Lima Silva  Berta Maria Heinzmann  Braulio Otomar Caron  Bernardo Baldisserotto 《Fish physiology and biochemistry》2014,40(3):701-714

The anesthetic activities of the essential oils (EOs) of Hesperozygis ringens (EOHR) and Lippia alba (EOLA) and their effects in silver catfish (Rhamdia quelen) after anesthesia and recovery were investigated. Fish (32.19 ± 1.24 g) were submitted to one of the following treatments for each EO: basal group, control, or anesthesia (150, 300, or 450 μL L^?1 EO). After that the anesthesia was induced or simulated and the biometric measurements were completed, fish were transferred to anesthetic-free aquaria to allow for recovery. Fish were sampled at 0, 15, 30, 60, and 240 min after recovery. At time 0 of recovery, the ventilatory rate was lower in the groups anesthetized with either EO. In comparison with the basal group, control fish showed an increase in plasma glucose, aspartate aminotransferase (AST), and Na⁺ levels and a reduction in Na⁺/K⁺-ATPase activity at 0 min of recovery. Plasma levels of ammonia and Na⁺ were lower in the fish anesthetized with EOLA (450 μL L^?1) and EOHR (all concentrations), respectively, than in the control fish. Additionally, lactate, AST, alanine aminotransferase, K⁺ plasma levels, and gill Na⁺/K⁺-ATPase and H⁺-ATPase activities were higher in the fish anesthetized with either EOHR or EOLA than in the control fish. The EOs promoted slight changes in silver catfish that enabled both an adaptive response and the recovery of most of the measured parameters after 240 min regardless of concentration or EO that was used. These findings support the use of EOHR and EOLA as anesthetics for fish.  相似文献   

Monitoring of eucalyptus sprouts control using digital images obtained by unmanned aerial vehicle     

Caique Carvalho Medauar  Samuel de Assis Silva  Luis Carlos Cirilo Carvalho  Rafael Augusto Soares Tibúrcio  Julião Soares de Souza Lima  Paullo Augusto Silva Medauar 《Journal of Sustainable Forestry》2018,37(7):739-752

This study evaluated the effectiveness of chemical control for eradicating eucalyptus sprouts using images obtained with Unmanned Aerial Vehicle (UAV). The study was carried out in eucalyptus plantations in Itabela, BA, in replanting areas during pre-planting application of herbicide. Aerial images obtained by a UAV were used to evaluate the effectiveness of the herbicide application for sprouts control. After the images were acquired, they were processed to calculate the normalized difference vegetation index (NDVI) and submitted to a supervised classification to quantify the percentage reduction of sprout green matter. The percentage data were submitted to analysis of variance and the means were compared by Tukey test at 5%. The differences, both visual and average percentages were observed only during the evaluation period with no evident effect of the treatments on sprout control. The images provided by the UAV allowed to monitor and identify visually the plots where biomass reduced or increased and to evaluate the effectiveness of chemical control of eucalyptus sprouts, indicating areas where it was nonexistent, partial or total.  相似文献   

The Impact of Organic Biofertilizer Application in Dairy Cattle Manure on the Chemical Properties of the Soil and the Growth and Nutritional Status of Urochroa Grass     

C. V. Soares Filho  J. F. Cavazzana  R. Heinrichs  J. M. B. Vendramini  G. C. Lima 《Communications in Soil Science and Plant Analysis》2018,49(3):358-370

The management of nitrogen (N) fertilization in pasture has great importance for yield and maintenance of Brazilian livestock. The objective of this study was to evaluate soil chemical attributes, shoot dry weight yield (SDWY) and roots dry weight yield (RDWY), nutritional status and nutritive value of Mulato II Urochloa grass that received organic biofertilizer rates as N source. The treatments were arranged in completely randomized blocks, were fertilized with six organic biofertilizer rates (0, 25, 50, 100, 200, and 400 m³ ha^?1) and five replicates during four harvest cycles. There was a quadratic response in the accumulated SDWY for up to a rate of 400 m³ ha^?1 and RDWY with 330 m³ ha^?1 of organic biofertilizer. The organic biofertilizer influenced the soil chemical attributes and foliar nutrient concentration. However, only the crude protein content presented a quadratic response, and neutral detergent fiber and acid detergent fiber levels were not affected by the influence of organic biofertilizer applied to the soil.  相似文献   

Effect of provision of apiaceous flowers associated to foods on the biology of <Emphasis Type="Italic">Coleomegilla maculata</Emphasis>     

Vinicius de Abreu D’Ávila  Elen de Lima Aguiar-Menezes  Renato Nunes Pereira  Vânia Gonçalves-Esteves  Cláudia Barbieri Ferreira Mendonça  Saulo José de Melo  Tamires Medeiros dos Santos 《Phytoparasitica》2017,45(4):471-484

Coleomegilla maculata DeGeer is an omnivorous lady beetle that feeds on natural or alternative prey and artificial foods, which allows its laboratory rearing for use on augmentative biocontrol. In addition, C. maculata may supplement its diet with pollen and nectar, which helps in its conservation in agroecosystems. This study aimed to evaluate if Apiaceous flowers (Anethum graveolens L. and Coriadrum sativum L.), with and without alternative prey [eggs of Ephestia kuehniella (Zeller) or larvae of Drosophila melanogaster Meigen] or an artificial food (aqueous solution of honey), may guarantee the survival and complete development of the immature and adult stages of C. maculata in the laboratory. The immature stages developed only when Apiaceous flowers were offered with E. kuehniella eggs. The food with only one of the alternative prey (moth eggs or fly larvae) or moth eggs + honey solution resulted in fertile adults; however, the number of eggs/cluster was greater for the foods with E. kuehniella eggs + honey solution, A. graveolens flowers, or only D. melanogaster. Foods comprising only the two Apiaceous species, only the honey solution, or only water resulted in larval development up to a specific instar. Adults of C. maculata also survived on these foods, but there was no oviposition. The foods of the two Apiaceous species produced heavier adults only when associated with E. kuehniella eggs. The results indicate that the zoophytophagous habit of C. maculata should be considered in conservation biocontrol programs aimed at using this lady beetle to control crop pests.  相似文献   

Nitrate Reductase,Micronutrients and Upland Rice Development as Influenced by Soil pH and Nitrogen Sources     

Edemar Moro  Carlos Alexandre Costa Crusciol  Heitor Cantarella  Fernando Broetto  Adriana Lima Moro 《Communications in Soil Science and Plant Analysis》2017,48(22):2642-2651

The average yield of upland rice under no-tillage system (NTS), a sustainable soil management, is lower than in conventional tillage (one plowing and two disking). One of the reasons given for this drop in crop grain yield would be the low-nitrate assimilation capacity of rice seedlings, due to the low activity of the nitrate reductase (NR) enzyme in the early development phase. A greenhouse experiment was conducted to evaluate the effects of the soil acidic and nitrogen source in the micronutrient concentrations, NR activity and grain yield of upland rice growing under NTS. The soil used in the experiment was an Oxisol. The experimental design was completely randomized in a factorial 3 × 4. Treatments consisted of three levels of soil acidity (high, medium, and low) combined with four nitrogen sources (nitrate, ammonium, ammonium + nitrification inhibitor, and control – without N fertilization). The reduction of soil acidity reduced the concentration of zinc and manganese in rice plants. Generally, the activity of the NR enzyme was higher in plants grown in soils with low acidity and fertilized with calcium nitrate. There was a greater response in growth and yield in rice plants grown in soils with high acidity. Under medium acidity, rice plants grown with ammonium sulfate were more productive (no differences were detected with the addition of the nitrification inhibitor).  相似文献   

Removal of Phenolic Compounds from Aqueous Solutions Using Sludge-Based Activated Carbons Prepared by Conventional Heating and Microwave-Assisted Pyrolysis     

Glaydson S. dos Reis  Matthew A. Adebayo  Carlos H. Sampaio  Eder C. Lima  Pascal S. Thue  Irineu A. S. de Brum  Silvio L. P. Dias  Flavio A. Pavan 《Water, air, and soil pollution》2017,228(1):33

Sludge-derived activated carbons (ACs) were prepared by conventional heating and microwave pyrolysis. The ACs were characterized using several analytical and functional techniques and used for removal of six phenolic compounds from aqueous solutions. The adsorbents exhibited similar features and possessed hydrophobic surfaces. The ACs were assigned mesoporous materials, with specific surface areas of up to 641 and 540 m² g^?1 for CAC-500 and MAC-980, respectively. The preliminary results indicated that phenol removal onto the ACs increased in the order: m-cresol?<?phenol?<?o-cresol?<?2-chrorophenol?<?2-nitrophenol?<?hydroquinone. Hydroquinone exhibited the highest adsorption capacity and was chosen to continue the remaining part of the experimental work—kinetic and isothermal studies. The adsorption kinetic and isotherm data were well described by the Avrami fractionary order and Redlich–Peterson models, respectively. The maximum amounts (Q _max) of hydroquinone adsorbed at 25 °C were too high, reaching 1218.3 and 1202.1 mg g^?1 for CAC-500 and MAC-980, respectively. The mechanism of adsorption was proposed in this work, and it was suggested that donor–acceptor complex and π–π interactions play major roles in the adsorption process. The adsorbents were also tested on simulated effluents. The two ACs displayed good efficiency for the treatment of industrial simulated effluents.  相似文献   

Nitrogen doses on physiological attributes and yield of sugarcane grown under subsurface drip fertigation     

Andressa Freitas de Lima Rhein 《Journal of plant nutrition》2017,40(2):227-238

Soil hydric availability and nitrogen fertilization are important environmental factors that influence sugarcane production. In the present study, the physiological attributes SPAD index, maximum photochemical efficiency of photosystem II (F_v/F_m), leaf area index (LAI), chlorophyll and carotenoid content, and sugarcane productivity were assessed under different nitrogen doses (0, 50, 100, 150, and 200 kg N ha^?1) applied in the form of urea via subsurface drip fertigation. The physiological attributes were determined 38, 121, 208, 291, and 381 days after the third harvest (DAH), and stalk and sugar productivity at 381 DAH. The 100 kg N ha^?1 dose has produced better results for the sugarcane physiological attributes. Increasing doses of nitrogen applied via subsurface drip fertigation increased the productivity of stalks and sugar considerably.  相似文献   

Losses of 15N-nitrogen by plant–soil system after herbicide application on black oat     

V. Damin  P. C. O. Trivelin  L. A. C. Moraes  I. P. Bruno  L. A. Figueiredo  A. B. Lima 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2017,67(3):202-207

Recent studies have demonstrated that the use of glyphosate or glufosinate-ammonium herbicides for some cover crop desiccation in conservationist systems could favor nitrogen (N) losses from the soil–plant system. In this context, the objective of this study was to evaluate the losses of N by the plant–soil system after the desiccation of black oat (Avena strigosa Schreb.) with the application of herbicides glyphosate, glufosinate-ammonium, or paraquat. Two greenhouse experiments were implemented using black oat plants fertilized with labeled (¹⁵N) ammonium nitrogen, and the N loss of the plant–soil system was quantified. The desiccation of black oat with glyphosate caused a reduction in root dry mass by approximately 60% in both experiments. The glyphosate and glufosinate-ammonium reduced the amount of N present in the aboveground portion; however, the paraquat herbicide did not modify it. None of the herbicides applied affected N losses in the black oat plant–soil system. However, 5–15% of the N applied as fertilizer was lost up to harvest. The results suggest that black oat can be used as a cover crop in direct sowing in order to avoid nitrogen losses in the soil–plant system.  相似文献   

Detection of <Emphasis Type="Italic">Brucella</Emphasis> sp. infection through serological,microbiological, and molecular methods applied to buffaloes in Maranhão State,Brazil     

Larissa Sarmento dos Santos  Joicy Cortez Sá  Diego Luiz dos Santos Ribeiro  Nancyleni Pinto Chaves  Juliana Pinto da Silva Mol  Renato Lima Santos  Tatiane Alves da Paixão  Alcina Vieira de Carvalho Neta 《Tropical animal health and production》2017,49(4):675-679

The aim of the current study is to diagnose Brucella spp. infection using methods such as serology, bacterial isolation, and molecular analysis in buffaloes bred in Maranhão State. In order to do so, 390 samples of buffalo serum were subjected to serological tests, to Rose Bengal Plate Test (RBPT) and to 2-mercaptoethanol (2-ME) combined with slow agglutination test (SAT). Vaginal swabs were collected from seropositive animals and subjected to bacterial isolation and to generic PCR. According to the serological test, 16 animals had a positive reaction to the confirmatory test (2-ME/SAT). As for bacterial isolation, three samples resulted in the isolation of Brucella spp.-characteristic colonies, which were confirmed through PCR. These results confirmed Brucella spp. infection in the buffalo herd from Maranhão State.  相似文献   

Effect of hot-iron disbudding on behaviour and plasma cortisol of calves sedated with xylazine     

G. Stilwell  R.C. Carvalho  M.S. Lima 《Research in veterinary science》2010,88(1):188-231

We investigated cortisol and behaviour for the first hour after hot-iron disbudding of calves aged 37 ± 4 days: disbudded after i/m xylazine (n = 10); disbudded after i/m xylazine and regional anaesthesia with lidocaine (n = 10); sham-disbudded after xylazine and lidocaine (n = 11); sham-disbudded after i/m saline and lidocaine (n = 10). Xylazine-treated groups had higher cortisol than saline-treated animals and showed no differences among them at any time. Sham-disbudded calves with xylazine had lower cortisol at 60 min compared with all other times. Xylazine-alone disbudded calves struggled more during the procedure than all other groups. Xylazine-alone disbudded calves showed more ear-flicks at 10, 25 and 40 min and head-shakes at 40 min than all other groups. We conclude that cortisol should not be used as an indicator of pain in disbudded calves while under the sedative effect of xylazine and that some behaviours during and after the procedure are useful in showing that xylazine alone does not control hot-iron disbudding pain.  相似文献