Visceral leishmaniasis in captive wild canids in Brazil   总被引：1，自引：1，他引：0  

Luppi MM  Malta MC  Silva TM  Silva FL  Motta RO  Miranda I  Ecco R  Santos RL 《Veterinary parasitology》2008,155(1-2):146-151

Visceral leishmaniasis (VL) is endemic in Belo Horizonte (State of Minas Gerais, Brazil). Leishmania sp. can naturally infect several species of mammals, and the domestic dog is the most important reservoir of the disease in South America. This report describes five cases of visceral leishmaniasis in Brazilian canids. Among 15 animals kept in captivity in a zoo in Belo Horizonte (State of Minas Gerais, Brazil), two animals, a bush dog (Spheotos venaticos) and a hoary zorro (Lycalopex vetulus) were serologically positive and developed clinical signs of VL, whereas three other canids, including a crab-eating fox (Cerdocyon thous), a maned wolf (Chrysocyon brachyurus), and a hoary zorro (Lycalopex vetulus) had positive serological results without clinical signs.  相似文献   

Interactions of Haemophilus parasuis and its LOS with porcine brain microvascular endothelial cells     

Bouchet B  Vanier G  Jacques M  Gottschalk M 《Veterinary research》2008,39(5):42

Haemophilus parasuis is a swine pathogen that causes Gl?sser's disease, which is characterized by polyserositis and meningitis. The pathogenesis of the H. parasuis infection is poorly understood. To cause meningitis, H. parasuis has to cross the blood-brain barrier (BBB) to gain access to the central nervous system (CNS). We recently showed that H. parasuis adheres to and invades porcine brain microvascular endothelial cells (PBMEC). The aim of this study was to evaluate the role of H. parasuis lipooligosaccharide (LOS) in the adhesion to PBMEC and to determine if H. parasuis (and/or its LOS) is able to induce apoptosis and activation of PBMEC. Results showed that adhesion of H. parasuis to PBMEC was partially mediated by LOS. Moreover, H. parasuis induces caspase-3-mediated apoptosis of PBMEC in a time--and dose--dependent manner, but its LOS did not seem to be involved in such a process. Furthermore, H. parasuis and, to a lesser extent, its LOS, was able to induce the release of IL-8 and IL-6 by PBMEC. Field strains of H. parasuis serotypes 4 and 5 induced similar levels of these inflammatory mediators. Our data suggest that H. parasuis uses cellular adhesion, induction of apoptosis and up-regulation of inflammatory mediators as mechanisms to invade the CNS via the BBB, and that LOS would play a certain but limited role in such pathological process.  相似文献   

Effect of Chemical or Electrical Activation of Bovine Oocytes on Blastocyst Development and Quality   总被引：2，自引：0，他引：2  

MP Milazzotto  WB Feitosa  ARS Coutinho  MD Goissis  VP Oliveira  MEOA Assumpção  JA Visintin 《Reproduction in domestic animals》2008,43(3):319-322

Activation of in vitro‐matured (IVM) oocytes is essential for successful embryo production following nuclear transfer (NT) or intracytoplasmic sperm injection (ICSI). This study was designed to compare the rates of blastocyst production and embryo quality (as measured by numbers of viable cells) following parthenogenetic activation with electrical pulse or the use of two different calcium ionophores, A23187 (CA) or ionomycin (IO), with or without the addition of bovine serum albumin (BSA). IVM oocytes with a first polar body were randomly allocated to five treatment groups: CA (5 μm CA, 5 min; n = 88), CA + BSA (5 μm CA, 5 min; BSA, 5 min; n = 90), IO (5 μm IO, 5 min; n = 91), IO + BSA (5 μm IO, 5 min; BSA, 5 min; n = 86) and EL (two pulses of 1.5 kV/cm, 20 μs; n = 120). Blastocyst rates were higher (p < 0.05) for CA (54.4%), IO (51.4%) and EL (54.5%) than for IO + BSA (18.3%). Treatment CA + BSA (39.8%) did not differ from the others. There was no difference (p > 0.05) among treatments in total number of cells. However, the percentage of viable cells was reduced in CA (49.9%), CA + BSA (45.8%), IO (64.9%), IO + BSA (50.9%) compared with EL (82.7%). In summary, the addition of BSA to the IO treatment had an adverse effect on blastocyst production rates. Although there was no difference between electrical stimulation and chemical activation on blastocyst production rates, electrical activation resulted in blastocysts with a higher percentage of viable cells.  相似文献   

Pharmacokinetics and pharmacodynamics of chlorpyrifos in male and female cattle after topical administration     

Picco EJ  Rubio MR  David DC  Rodríguez C  Boggio JC 《Veterinary research communications》2008,32(5):401-410

The aim of this work was to study the pharmacokinetic behaviour and the inhibitory effect on acetylcholinesterase and butyrylcholinesterase activities of chlorpyrifos in male and female cattle after pour-on administration. Determination of cholinesterase activity in plasma and erythrocyte was carried out according to Ellman kinetic method. The mean baseline activities were 9338.39 ± 1331.61 and 13220.69 ± 2274.18 to acetylcholinesterase and 624.65 ± 39.32 and 641.68 ± 88.08 IU/L to butyrylcholinesterase in females and males, respectively. Acetylcholinesterase was the predominant form of cholinesterase analyzed, with low levels of butyrylcholinesterase. The basal acetylcholinesterase activities of the bulls were significantly greater than those of cows. The inhibitory effect of topical chlorpyrifos administration was lower on butyrylcholinesterase than on acetylcholinesterase. Chlorpyrifos peak plasma concentration (male:10.920 ± 4.18; female:12.12 ± 3.88 μg/L) were reached at 11.92 ± 9.19 and 8.17 ± 7.67 h in male and female, respectively. The values of area under curve were 185.96 ± 168.45 and 278.89 ± 270.00 μg·h/L and mean residence time were 13.95 ± 8.10 and 14.90 ± 9.80 h in male and female, respectively.  相似文献   

Dairy science and health in the tropics: challenges and opportunities for the next decades     

Hernández-Castellano  Lorenzo E.  Nally  Jarlath E.  Lindahl  Johanna  Wanapat  Metha  Alhidary  Ibrahim A.  Fangueiro  David  Grace  Delia  Ratto  Marcelo  Bambou  Jean Christophe  de Almeida  André M. 《Tropical animal health and production》2019,51(5):1009-1017

In the next two decades, the world population will increase significantly; the majority in the developing countries located in the tropics of Africa, Asia, Latin America, and the Caribbean. To feed such a population, it is necessary to increase the availability of food, particularly high-value animal protein foods produced locally, namely meat and dairy products. Dairy production in tropical regions has a lot of growth potential, but also poses a series of problems, particularly as dairy production systems were developed in temperate countries and in most cases are difficult to implement in the tropics. Drawbacks include hot weather and heat stress, the lack of availability of adequate feeds, poor infrastructure, and cold chain and the competition with cheap imports from temperate countries. This position paper reviews the major drawbacks in dairy production for the five major dairy species: cattle, water buffalo, sheep, goat, and camel, as well as the future trends in research and development. It also concerns the major trends in reproduction and production systems and health issues as well as environmental concerns, particularly those related to greenhouse gas emissions. Tropical Animal Health and Production now launches a topical collection on Tropical Dairy Science. We aim to publish interesting and significant papers in tropical dairy science. On behalf of the editorial board of the Tropical Animal Health and Production, we would like to invite all authors working in this field to submit their works on this topic to this topical collection in our journal.

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Carcass characteristics and meat quality of sheep fed alfalfa hay to replace Bermuda grass hay     

da Silva  José Ricardo Coelho  de Carvalho  Francisco Fernando Ramos  de Andrade Fereira  Marcelo  de Souza  Evaristo Jorge Oliveira  Maciel  Maria Inês Sucupira  Barreto  Lígia Maria Gomes  Lopes  Levi Auto  Cordeiro  Eduardo Henrique Araújo  Véras  Antonia Sherlânea Chaves 《Tropical animal health and production》2019,51(8):2455-2463

Tropical Animal Health and Production - The objective of this study was to evaluate carcass characteristics, meat quality, and organ and viscera weight of forty male sheep, not castrated, initially...  相似文献   

Intranasal Osteosarcoma in a Dog—A Case Report     

Gabriela Noronha de Toledo  Pamela Rodrigues Reina Moreira  Daniele Santos Rolemberg  Andréia Coutinho Facin  Julio Edward Hough Monteiro  Mareliza Possa de Menezes  Maria Eduarda Bastos Andrade Moutinho da Conceição  Felipe Augusto Ruiz Sueiro  Rosemeri de Oliveira Vasconcelos  Paola de Castro Moraes  Mirela Tinucci Costa  Andrigo Barboza de Nardi 《Topics in companion animal medicine》2018,33(2):35-39

Osteosarcoma is the most commonly reported bone tumor in dogs, typically affecting the axial and appendicular skeleton. Involvement of the paranasal sinuses and the nasal cavity is extremely rare. This report describes a 6-year-old, female, cross-breed dog presented with signs of 90 days duration and diagnosed with a mass located in the right nasal cavity. Radiography, rhinoscopy, and computed tomography findings suggested the presence of an intranasal tumor. Osteosarcoma in the nasal cavity was diagnosed based on histopathological examination and clinical and imaging findings. Survival time was 12 months from diagnosis.  相似文献   

Whelping and Dystocia: Maximizing Success of Medical Management     

Erin E. Runcan  Marco A. Coutinho da Silva 《Topics in companion animal medicine》2018,33(1):12-16

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A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil     

Labruna MB  McBride JW  Camargo LM  Aguiar DM  Yabsley MJ  Davidson WR  Stromdahl EY  Williamson PC  Stich RW  Long SW  Camargo EP  Walker DH 《Veterinary parasitology》2007,143(2):189-195

A molecular epidemiologic investigation in two Brazilian states (Rondônia and São Paulo) was undertaken to determine if Ehrlichia species responsible for human and animal ehrlichioses in North America could be found in Brazilian vectors, potential natural mammalian reservoirs and febrile human patients with a tick bite history. Samples, including 376 ticks comprising 9 Amblyomma species, 29 capybara (Hydrochaeris hydrochaeris) spleens, 5 canine blood, and 75 human blood samples from febrile patients with history of tick bites were tested by a real-time PCR assay targeting a fragment of the Ehrlichia dsb gene. Ehrlichia DNA was not detected in any tick, capybara or human samples. In contrast, 4 out of 5 dogs contained Ehrlichia canis DNA in their blood, which were sequenced, representing the first report of E. canis infecting dogs in the Amazon region of Brazil. Further studies are needed to evaluate the presence of other agents of human and animal ehrlichioses in Brazil.  相似文献   

Effects of Sewage on the Chemical Composition of Piracicaba River,Brazil     

Martinelli  Luiz A.  Krusche  Alex V.  Vicgoria  Reynaldo L.  De Camargo  Plinio B.  Bernardes  Marcelo  Ferraz  Epaminondas S.  De Moraes  Jorge M.  Ballester  Maria Victoria 《Water, air, and soil pollution》1999,110(1-2):67-79

Water samples were collected from 7 locations along major rivers of Piracicaba River basin for 22 months. The 4 upstream points represent non-polluted sites and the 3 downriver points represent polluted sites. Due to sewage input, concentrations of dissolved organic carbon (DOC), dissolved inorganic carbon (DIC), major conservative cations and anions increased significantly in the polluted sites. The major increases were observed for sodium, chloride and sulfate. Nitrate was an exception for this pattern, with similar concentrations between polluted and non-polluted sites. The probable cause was reduction of nitrate to ammonium in the polluted sites, where anoxic conditions prevail. Most of the variables had an inverse correlation with water discharge, especially in the polluted points. The sewage load was diluted by precipitation and surface waters.  相似文献