Microbiological and chemical characterization of a typical Italian cheese: Robiola di Roccaverano     

Bonetta S  Coïsson JD  Barile D  Bonetta S  Travaglia F  Piana G  Carraro E  Arlorio M 《Journal of agricultural and food chemistry》2008,56(16):7223-7230

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Enrichment and diversity of plant-probiotic microorganisms in the rhizosphere of hybrid maize during four growth cycles     

Christine Picard  Elisa Baruffa  Marco Bosco 《Soil biology & biochemistry》2008,40(1):106-115

The population dynamics of naturally-occurring antibiotic producing and nitrogen fixing rhizobacteria, as well as of arbuscular mycorrhizal fungi (AMF) was investigated for a hybrid of maize (Lo964×Lo1016) in comparison to its two parental lines (Lo964 and Lo1016), during four successive 5-week-long growth cycles in the same pot. Beneficial rhizobacteria, such as nitrogen fixers and 2,4-diacetylphloroglucinol (DAPG) and pyrrolnitrin (PRN) producers, as well as AMF were stimulated in the hybrid rhizosphere earlier than in those of the two parental lines. In fact, they were molecularly detected in all rhizospheric samples of the hybrid, independently of the cycle, whereas for the parental lines positive detections occurred only for samples collected after at least two growth cycles. Interestingly, a MPN-PCR approach on rhizospheric DNA samples indicated that, when detected, beneficial rhizobacteria reached similar density in all maize genotypes (2×10³ to 2.2×10⁴ target DNA sequences/g^?1 of root). Concerning the AMF, even if the three maize genotypes were cultivated in the same soil, it appears that each maize genotype stimulates the AMF population differently. Both the hybrid and the Lo964 line were able to select, in the observed time period, their own adapted phylogenetic AMF subgroups (Glomus A for the hybrid, Archeospora for Lo964), whereas the Lo1016 line was not.  相似文献   

Oral cavity evaluation and dental chart registration of coati (Nasua nasua) in captivity     

Freitas EP  Rahal SC  Teixeira CR  Teixeira RH  Mendes GM  Gioso MA 《Journal of veterinary dentistry》2008,25(2):110-117

The aims of this study were to develop a dental evaluate any oral cavity disease, develop gypsum models of the dental arches, and to register the occlusions found in coatis (Nasua nasua) in captivity Formulation of the dental chart was assisted by intraoral radiographs from the head of an adult coati cadaver of the same species with the following dental formula.: I 3/3, C 1/1, P 4/3, M 2/2. Seven live coatis of the Nasua nasua species were evaluated. Five of the seven coatis presented with various dental abnormalities as follows: dental plaque (71.4 0%), gingivitis (71.4 %), periodontitis (57.1 %), dental stain (42.9 %), dental abrasion (57.1 %), dental fracture (57.1 %), pulp exposure (42.9 %), malocclusion (57.1 %) and supernumerary teeth (14.2 %).  相似文献   

Evaluation of EDTA hematology tubes for collection of blood samples for tests of secondary hemostasis in dogs     

Cerón JJ  Carli E  Tasca S  Martinez-Subiela S  Caldin M 《American journal of veterinary research》2008,69(9):1141-1147

OBJECTIVE: To evaluate the use of EDTA tubes for collection of blood samples for assays of secondary hemostasis in dogs. ANIMALS: 108 dogs of various ages, breeds, and sexes (19 healthy and 89 with abnormalities of secondary hemostasis). PROCEDURES: Blood samples were collected via cephalic venipuncture and transferred to sodium citrate tubes and EDTA tubes. Plasma was harvested from each type of tube for assays of concentrations of fibrinogen and D-dimer as well as prothrombin time, activated partial thromboplastin time, and antithrombin activity. Intra-assay and interassay precision and correlation coefficients for all hemostatic tests were calculated for each type of plasma sample. The effect of storage conditions on assay results for the 2 types of plasma samples was also evaluated. RESULTS: Results of hemostatic tests were highly correlated between citrated and EDTA-treated plasma samples. Intra-assay imprecision for all hemostatic tests with the exception of D-dimer concentration was < 10% for both citrated and EDTA-treated plasma samples; interassay imprecision was higher for EDTA-treated versus citrated plasma samples. Storage of plasma samples for 1 hour did not result in significantly different assay results for either type of plasma sample, but storage for 2 hours significantly affected values for EDTA-treated plasma samples. CONCLUSIONS AND CLINICAL RELEVANCE: Although evaluation of the sensitivity and specificity of hemostatic tests that use EDTA-treated plasma samples is required, EDTA may be a suitable alternative to sodium citrate as an anticoagulant for use in hemostatic testing in conditions in which tests could be performed within 1 hour after sample collection.  相似文献   

家禽养殖中的水质控制     

Marco Quiroz 《中国家禽》2008,30(12):49-50

虽然鸡群中推荐使用水冲洗去除污染物质,但是冲洗不能去除黏液层或者细菌生物膜或者藻类.因此,更多的家禽养殖者添加一些有机酸到饮水和冲洗程序中.这除了抑制饮水系中生物膜形成,还会减少有害细菌,改善动物生长性能和食品安全.  相似文献   

KIT-positive gastrointestinal stromal tumours in two Spanish ibex (Capra pyrenaica hispanica)     

Velarde R  Mentaberre G  Sánchez J  Marco I  Lavín S 《Veterinary journal (London, England : 1997)》2008,177(3):445-447

Gastrointestinal mesenchymal tumours from two Spanish ibex (Capra pyrenaica hispanica) were examined grossly, histologically and immunohistochemically. One neoplasm was a 1.5 kg tan multinodular cavitated mass in the forestomach. The other tumour was a firm mural mass 1.2 cm in diameter in the colon. Microscopically, both tumours were formed mainly by spindle shaped cells arranged in closely packed interlacing fascicles. Neoplastic cells in both tumours labelled positively for KIT (CD117), vimentin and alpha-smooth muscle actin. These findings suggest that both neoplasms were gastrointestinal stromal tumours and most likely to be derived from the interstitial cells of Cajal or their progenitor cells.  相似文献   

Preliminary results on the association with feeding and recovery length in equine colic patients after laparotomy     

Emanuela Valle  Gessica Giusto  Livio Penazzi  Marzia Giribaldi  Domenico Bergero  Maria Joo Fradinho  Luís Ressano Garcia P. Lamas  Marco Gandini 《Journal of animal physiology and animal nutrition》2019,103(4):1233-1241

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Comparison of the intravenous and intravaginal route of oxytocin administration for cervical dilation protocol and non‐surgical embryo recovery in oestrous‐induced Santa Inês ewes     

Lucia Prellwitz  Fabiana Nunes Zambrini  Jos Domingos Guimares  Marco Antonio Paula de Sousa  Maria Emília Franco Oliveira  Alexandre Rosetto Garcia  Srgio Novita Esteves  Pawel Mieczyslaw Bartlewski  Joanna Maria Gonalves Souza‐Fabjan  Jeferson Ferreira Fonseca 《Reproduction in domestic animals》2019,54(9):1230-1235

This study compared the effects of intravaginal and intravenous routes of oxytocin (OT) administration in 46 oestrous‐induced Santa Inês ewes (6‐day treatment with progestin‐releasing intravaginal sponges and a single injection of 200 IU of eCG at the time of sponge removal) that underwent transcervical embryo recovery 6–7 days after oestrous onset and mating. All ewes received 37.5 μg of d‐cloprostenol via latero‐vulvar route, and 1 mg of oestradiol benzoate i.m. 16 hr before and 50 IU of OT 20 min before non‐surgical embryo recovery (NSER), with OT being administered intravenously (n = 21) or intravaginally (n = 21). An overall oestrous response was 95.6% (44/46), and adequate cervical retraction could be accomplished in 78.6% (33/42) of ewes. The percentage of successful NSER procedures was 57% (24/42) or 72.7% (24/33) of animals with sufficient cervical retraction. The duration of NSER procedure averaged 28 min (range: 17–40 min) and ~96% of flushing fluid could be recovered (range: 85%–100%). Out of 18 ewes that could not undergo NSER, 12 (66.6%) presented various anatomical barriers, whilst the other 33.4% did not present these barriers and still could not be traversed. Excluding the ewes with those anatomical features, the overall success rate of NSER was 80% (24/30). The route of OT administration had no effect on NSER efficiency or the ease with which transcervical embryo flushing was performed. Both routes of OT administration can be used for cervical dilation protocol. Discarding ewes with anatomical features precluding cervical penetration is highly recommended to increase the efficacy of NSER in sheep.  相似文献   

Listeria associated mural and valvular endocarditis in an alpaca     

Megan J. McLane DVM  John W. Schlipf Jr. DVM  MS  Marco L. Margiocco DMV  MS  Howard Gelberg DVM  PhD 《Journal of Veterinary Cardiology》2008,10(2):141-145

An alpaca presented for emergency evaluation of respiratory distress. Physical examination revealed weakness, pyrexia, and a grade V/VI left apical holosystolic murmur. Initial thoracic examination and radiographs were consistent with left sided congestive heart failure. Blood cultures, echocardiographic evaluation, and necropsy findings established a diagnosis of bacterial endocarditis associated with a Listeria monocytogenes infection. Left ventricular endocarditis is uncommon in ruminants, and to the authors' knowledge there are no other reports of L. monocytogenes being the causative agent in an alpaca.  相似文献   

Whelping and Dystocia: Maximizing Success of Medical Management     

Erin E. Runcan  Marco A. Coutinho da Silva 《Topics in companion animal medicine》2018,33(1):12-16

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