In vivo morphological and antigenic characteristics of Photobacterium damselae subsp. piscicida     

Tae S. Jung  Kim D. Thompson  Donatella Volpatti  Marco Galeotti  A. Adams 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(2):169-175

The present study was conducted to examine the morphology and antigenicity of Photobacterium damselae subsp. piscicida by culturing the bacterium in vivo in the peritoneal cavity of sea bass (Dicentrarchus labrax) within dialysis bags with either a low molecular weight (LMW) cut-off of 25 kDa or a high molecular weight (HMW) cut-off of 300 kDa. Differences were observed in the growth rate between the bacteria cultured in vivo or in vitro. Bacteria cultured in vivo were smaller and produced a capsular layer, which was more prominent in bacteria cultured in the HMW bag. Antigenicity was examined by Western blot analysis using sera from sea bass injected with live Ph. d. subsp. piscicida. The sera recognised bands at 45 and 20 kDa in bacteria cultured in vivo in the LMW bag. Bacteria cultured in vivo in the HMW bag did not express the 45 kDa band when whole cell extracts were examined, although the antigen was present in their extracellular products. In addition, these bacteria had a band at 18 kDa rather than 20 kDa. Differences in glycoprotein were also evident between bacteria cultured in vitro and in vivo. Bacteria cultured in vitro in LMW and HMW bags displayed a single 26 kDa band. Bacteria cultured in the LMW bag in vivo displayed bands at 26 and 27 kDa, while bacteria cultured in vivo in the HMW bag possessed only the 27 kDa band. These bands may represent sialic acid. The significance of the changes observed in the bacterium''s structure and antigenicity when cultured in vivo is discussed.  相似文献   

Determining optimal therapy of dogs with chronic enteropathy by measurement of serum citrulline          下载免费PDF全文

Magda Gerou‐Ferriani  Rhiannon Allen  Peter‐John M. Noble  Alexander J. German  Marco Caldin  Daniel J. Batchelor 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):993-998

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PET AND CAPTIVE BIRDS AS POTENTIAL RESERVOIRS OF ZOONOTIC BACTERIA     

Carlotta De Luca  Giulia Niero  Diego Cattarossi  Marco Bedin  Alessandra Piccirillo 《Journal of Exotic Pet Medicine》2018,27(1):17-20

Thermophilic Campylobacter spp. and Salmonella spp. are zoonotic bacteria, commonly harbored in the enteric tract of avian species. This survey aimed at verifying the presence of these microorganisms in a heterogeneous Italian population of pet birds and captive birds of prey (e.g., Passeriformes, Psittaciformes, Accipitriformes, Falconiformes, and Strigiformes) that live in close contact with humans. A total of 151 individuals were tested for thermophilic Campylobacter spp. and 444 for Salmonella spp. Six C. jejuni and one S. Livingstone were isolated from birds of prey: 4 Accipitriformes (2 Buteo regalis, 2 Parabuteo unicinctus), 1 Falconiformes (Falco peregrinus) and 1 Strigiformes (Strix aluco) were positive for C. jejuni, and 1 Falconiformes (Falco peregrinus) for S. Livingstone. Five C. jejuni strains were genotyped by Multilocus Sequence Typing (MLST) and identified as ST 2116, assigned to ST-353 CC. Results of this study suggest that these microorganisms are not common inhabitants of the digestive tract of pet birds and captive birds of prey, but occasional findings. However, as potential reservoirs of zoonotic bacteria, pet birds should be monitored in order to preserve human health.  相似文献   

MESENTERIC HETEROTOPIC OSSIFICANS IN A FERRET (MUSTELA PUTORIUS FURO): A RARE CAUSE OF SOFT TISSUE OSSIFICATION     

Marco Tecilla  Mattia Bielli  Paolo Savarino  Paola Roccabianca 《Journal of Exotic Pet Medicine》2018,27(2):108-112

Heterotopic ossification (HO) is a rare condition characterized by abnormal bone production in soft tissue. Very few cases of HO have been reported in animals (e.g., dogs and horses) and this disease process is often a consequence of a traumatic incident. A 5-year-old, male European ferret (Mustela putorius furo) was referred for anorexia, generalized weakness, and weight loss. Clinical examination revealed multiple abdominal masses. An explorative laparotomy identified omental, white/tan, nodular to coalescing hard lesions. The omentum and spleen were surgically removed during the surgical procedure, with both subsequently submitted for histopathologic evaluation. Microscopically, the omental masses were composed of a meshwork of lamellar and woven bone admixed with islands of cartilage and lacunae containing either blood, fat, and/or bone marrow. Multifocal mineralization and bone lamellae were also present in the grossly normal omentum. The finding of multiple bone nodules containing bone marrow and/or adipose tissue, with no Harversian system, and minimal intratrabecular connective tissue favored a diagnosis of HO.  相似文献   

Erythrocyte osmotic fragility and select hematologic variables in postparturient mares and their foals          下载免费PDF全文

Francesca Arfuso  Marco Quartuccio  Marilena Bazzano  Francesco Fazio  Giuseppe Piccione 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(2):260-270

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Thrombocytopenia in Japanese Akita dogs          下载免费PDF全文

Marco Caldin  Andrea Zoia  Silvia Tasca  Tommaso Furlanello 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(3):397-397

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Correction of neonatal stifle luxation in a 35‐day‐old cockatiel (Nymphicus hollandicus)          下载免费PDF全文

Marco Luparello  Laura Faraci  Marco Di Giuseppe  Lorenzo Crosta 《The Journal of small animal practice》2016,57(11):653-654

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Degradation of dicarboximidic fungicides in wine. Part II: Isolation and identification of the major breakdown products of chlozolinate,vinclozolin and procymidone     

Filippo M. Pirisi  Marco Meloni  Paolo Cabras  Maria R. Bionducci  Adriana Serra 《Pest management science》1986,17(2):109-118

The main degradation products formed from the dicarboximidic fungicides chlozolinate, vinclozolin and procymidone in wine have been isolated and identified using spectroscopic and chromatographic methods. The fungicides were added to wine after fermentation. Chlozolinate underwent a rapid hydrolytic loss of the ethoxy-carbonyl substituent, to give an oxazolidine that underwent hydrolytic cleavage to give 3′,5′-dichloro-2-hydroxypropanilide. The oxazolidine ring of vinclozolin underwent a similar hydrolysis to give the corresponding anilide 3′,5′-dichloro-2-hydroxy-2-methylbut-3-enanilide. Both these anilides were stable in wine for 150 days. A different degradation behaviour was observed with procymidone and led to the formation of 3,5-dichloroaniline (3,5-DCA), which, in turn, broke down but the derivatives could not be isolated. After consideration of the different behaviours of the fungicides on degradation in wine and in aqueous ethanol at pH4, together with their kinetic data, breakdown pathways are proposed.  相似文献   

Lambing rate and prolificacy in inseminated hair sheep treated with bovine somatotropin     

José Maria Carrera-Chávez  Joel Hernández-Cerón  Carlos Fernando Aréchiga-Flores  Marco Antonio López-Carlos  Raúl Renato Lozano-Domínguez  Andrés Quezada-Casasola  Francisco Guadalupe Echavarría-Cháirez 《Tropical animal health and production》2016,48(4):699-703

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Hospitalization disrupts the daily rhythm of tear production in cats     

Simona Di Pietro  Marco Tabbì  Annastella Falcone  Francesco Macrì  Giuseppe Piccione  Elisabetta Giudice  Claudia Giannetto 《Veterinary ophthalmology》2023,26(Z1):119-124

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